Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan...Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan 96-3(ZY96-3),an elite germplasm known for faster grain filling and superior grain size.A genotyping-by-sequencing(GBS)approach was applied to an F_(2)population of 110 individuals derived from GZ1×ZY96-3,resulting in the identification of 23,134 high-quality SNPs.Most of the SNPs associated with GL and GW were clustered on chromosomes 2B,3A,and 3B.QTL mapping for GL revealed two major loci,GL1 on chromosome 2B and GL2 on chromosome 3B,and eight candidate genes were identified within their corresponding intervals(2B:63.6–70.4 Mb;3B:631.5–633.3 Mb).These genes encode proteins potentially involved in grain size regulation,including a TOR2 regulation-associated protein,erect spike 2(EP2),fibroblast growth factor 6(FGF6),cellulose synthase-like(CSLD),RelA/pot homologue three family protein,and three GDSL esterase/lipase(GLIP)proteins.Additionally,we detected a QTL associated with GW on chromosome 3A and identified two candidate genes,TOR2 regulation and starch synthase within the 61.4–68.5 Mb interval.Overall,this study provides a strong theoretical and technical basis for wheat genetic improvement and offers valuable resources for precise QTL mapping and candidate gene discovery.展开更多
基金Funding for this project was provided by the National Natural Science Foundation of China(Grants No.32160456,32360474,32360486,32260496)the Key Laboratory of Functional Agriculture of Guizhou Provincial Higher Education Institutions(Grant No.Qianjiaoji(2023)007).
文摘Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan 96-3(ZY96-3),an elite germplasm known for faster grain filling and superior grain size.A genotyping-by-sequencing(GBS)approach was applied to an F_(2)population of 110 individuals derived from GZ1×ZY96-3,resulting in the identification of 23,134 high-quality SNPs.Most of the SNPs associated with GL and GW were clustered on chromosomes 2B,3A,and 3B.QTL mapping for GL revealed two major loci,GL1 on chromosome 2B and GL2 on chromosome 3B,and eight candidate genes were identified within their corresponding intervals(2B:63.6–70.4 Mb;3B:631.5–633.3 Mb).These genes encode proteins potentially involved in grain size regulation,including a TOR2 regulation-associated protein,erect spike 2(EP2),fibroblast growth factor 6(FGF6),cellulose synthase-like(CSLD),RelA/pot homologue three family protein,and three GDSL esterase/lipase(GLIP)proteins.Additionally,we detected a QTL associated with GW on chromosome 3A and identified two candidate genes,TOR2 regulation and starch synthase within the 61.4–68.5 Mb interval.Overall,this study provides a strong theoretical and technical basis for wheat genetic improvement and offers valuable resources for precise QTL mapping and candidate gene discovery.
