Plant height(PH)is associated with lodging resistance and planting density,which is regulated by a complicated gene network.In this study,we identified a spontaneous dwarfing mutation in maize,m30,with decreased inter...Plant height(PH)is associated with lodging resistance and planting density,which is regulated by a complicated gene network.In this study,we identified a spontaneous dwarfing mutation in maize,m30,with decreased internode number and length but increased internode diameter.A candidate gene,ZmCYP90D1,which encodes a member of the cytochrome P450 family,was isolated by map-based cloning.ZmCYP90D1 was constitutively expressed and showed highest expression in basal internodes,and its protein was targeted to the nucleus.A G-to-A substitution was identified to be the causal mutation,which resulted in a truncated protein in m30.Loss of function of ZmCYP90D1 changed expression of hormoneresponsive genes,in particular brassinosteroid(BR)-responsive genes which is mainly involved in cell cycle regulation and cell wall extension and modification in plants.The concentration of typhasterol(TY),a downstream intermediate of ZmCYP90D1 in the BR pathway,was reduced.A haplotype conferring dwarfing without reducing yield was identified.ZmCYP90D1 was inferred to influence plant height and stalk diameter via hormone-mediated cell division and cell growth via the BR pathway.展开更多
目的探讨大脑中动脉急性闭塞血管内治疗(EVT)患者的血栓渗透性与临床功能预后的关系并分析血栓渗透性、侧支循环与临床功能预后之间的效应关系。方法纳入2018年1月—2024年1月延安大学附属医院心脑血管病医院大脑中动脉闭塞并接受EVT的...目的探讨大脑中动脉急性闭塞血管内治疗(EVT)患者的血栓渗透性与临床功能预后的关系并分析血栓渗透性、侧支循环与临床功能预后之间的效应关系。方法纳入2018年1月—2024年1月延安大学附属医院心脑血管病医院大脑中动脉闭塞并接受EVT的急性缺血性脑卒中(AIS)患者77例行回顾性分析,根据血栓渗透性高与低分为高渗透性组(44例)和低渗性组(33例)。研究的主要终点是90 d改良Rankin量表(mRS)良好功能结局(mRS 0~3分),次要终点为术后24 h美国国立卫生研究院卒中量表(NIHSS)评分、(7±2)d NIHSS评分、早期神经系统改善(ENI)、90 d mRS功能独立(mRS 0~2分)、90 d mRS极佳的功能恢复(mRS 0~1分)。进一步评估侧支循环,采用中介效应分析来评估侧支循环对血栓渗透性和90 d mRS功能预后之间的效应关系。结果高渗透性组较低渗透性组低密度脂蛋白低,术前NIHSS评分及ASPECTS评分高,侧支循环较好(均P<0.05)。高渗透性组患者在90 d时获得良好功能结局[mRS(0~3分)]、实现功能独立的可能性mRS[(0~2分)]及极佳的功能恢复方面的几率显著高于低渗透性组(P<0.001)。血栓渗透性和侧支循环与功能结局无显著交互作用(P=0.352)。血栓渗透性对侧支循环的效应(a=0.0190,P=0.0017),侧支循环对mRS的效应(b=0.9656,P=0.0000),血栓渗透性对90 d mRS的总效应(C=0.0303,P=0.0005),引入侧支循环的直接效应(C′=0.0119,P=0.0737),且中介效应值的bootstrap 95%置信区间(0.0251,0.0012)不包含0,均表明存在中介效应。结论大脑中动脉急性闭塞进行EVT患者中,高渗透性血栓患者比低渗透性血栓患者获得更好的90 d mRS。引入的侧支循环变量在血栓渗透性与90 d mRS良好功能结局之间存在完全中介效应。展开更多
基金This work was supported by the National Natural Science Foundation of China(U2004144,31971893,32101743)the Key Technologies R&D Program of Henan Province(232102111080)Yunnan Academician Expert Workstation(202305AF150082).
文摘Plant height(PH)is associated with lodging resistance and planting density,which is regulated by a complicated gene network.In this study,we identified a spontaneous dwarfing mutation in maize,m30,with decreased internode number and length but increased internode diameter.A candidate gene,ZmCYP90D1,which encodes a member of the cytochrome P450 family,was isolated by map-based cloning.ZmCYP90D1 was constitutively expressed and showed highest expression in basal internodes,and its protein was targeted to the nucleus.A G-to-A substitution was identified to be the causal mutation,which resulted in a truncated protein in m30.Loss of function of ZmCYP90D1 changed expression of hormoneresponsive genes,in particular brassinosteroid(BR)-responsive genes which is mainly involved in cell cycle regulation and cell wall extension and modification in plants.The concentration of typhasterol(TY),a downstream intermediate of ZmCYP90D1 in the BR pathway,was reduced.A haplotype conferring dwarfing without reducing yield was identified.ZmCYP90D1 was inferred to influence plant height and stalk diameter via hormone-mediated cell division and cell growth via the BR pathway.
文摘目的探讨大脑中动脉急性闭塞血管内治疗(EVT)患者的血栓渗透性与临床功能预后的关系并分析血栓渗透性、侧支循环与临床功能预后之间的效应关系。方法纳入2018年1月—2024年1月延安大学附属医院心脑血管病医院大脑中动脉闭塞并接受EVT的急性缺血性脑卒中(AIS)患者77例行回顾性分析,根据血栓渗透性高与低分为高渗透性组(44例)和低渗性组(33例)。研究的主要终点是90 d改良Rankin量表(mRS)良好功能结局(mRS 0~3分),次要终点为术后24 h美国国立卫生研究院卒中量表(NIHSS)评分、(7±2)d NIHSS评分、早期神经系统改善(ENI)、90 d mRS功能独立(mRS 0~2分)、90 d mRS极佳的功能恢复(mRS 0~1分)。进一步评估侧支循环,采用中介效应分析来评估侧支循环对血栓渗透性和90 d mRS功能预后之间的效应关系。结果高渗透性组较低渗透性组低密度脂蛋白低,术前NIHSS评分及ASPECTS评分高,侧支循环较好(均P<0.05)。高渗透性组患者在90 d时获得良好功能结局[mRS(0~3分)]、实现功能独立的可能性mRS[(0~2分)]及极佳的功能恢复方面的几率显著高于低渗透性组(P<0.001)。血栓渗透性和侧支循环与功能结局无显著交互作用(P=0.352)。血栓渗透性对侧支循环的效应(a=0.0190,P=0.0017),侧支循环对mRS的效应(b=0.9656,P=0.0000),血栓渗透性对90 d mRS的总效应(C=0.0303,P=0.0005),引入侧支循环的直接效应(C′=0.0119,P=0.0737),且中介效应值的bootstrap 95%置信区间(0.0251,0.0012)不包含0,均表明存在中介效应。结论大脑中动脉急性闭塞进行EVT患者中,高渗透性血栓患者比低渗透性血栓患者获得更好的90 d mRS。引入的侧支循环变量在血栓渗透性与90 d mRS良好功能结局之间存在完全中介效应。
