To explore the molecular mechanisms by which autophagy contributes to pepper's heat tolerance, we previously identified the zinc-finger protein B-BOX 9/CONSTANS-LIKE 13(CaBBX9/CaCOL13) as an interaction partner of...To explore the molecular mechanisms by which autophagy contributes to pepper's heat tolerance, we previously identified the zinc-finger protein B-BOX 9/CONSTANS-LIKE 13(CaBBX9/CaCOL13) as an interaction partner of the autophagy related protein(ATG) CaATG8c, a core component in autophagy. However, the involvement of CaBBX9 in both autophagy and heat tolerance remains unclear. In this study, we further confirmed the interaction between CaBBX9 and CaATG8c and defined the interaction regions of CaBBX9 as CONSTANS, CONSTANS-Like, and TOC1(CCT) domain and the fragment region. The expression of CaBBX9 can be induced by heat treatment. CaBBX9 is co-localized with CaATG8c in the nucleus and exhibits a transcriptional activity. When the expression of CaBBX9 is silenced, the heat tolerance of pepper is enhanced, shown by the decrement of MDA content, H2O2and dead cells accumulation, and relative electrolyte leakage, along with the increment of chlorophyll content and expression level of heat-tolerance-related genes. Overexpression of CaBBX9 in tomatoes displays the opposite effects. Taken together, we demonstrate that CaBBX9 negatively regulates the heat tolerance of peppers by exacerbating oxidative damage and inhibiting the expression of heat-related genes. Our findings provide a new clue for guiding crop breeding for pepper tolerance to heat stress.展开更多
大豆是一种高需氮素作物,其籽粒发育所需氮素在很大程度上来源于"源"器官中氮素的再动员,而细胞自噬是植物进行氮素再动员的主要形式之一。为了研究大豆细胞自噬关键基因GmATG8c的功能,本文利用35S启动子驱动GmATG8c基因在大...大豆是一种高需氮素作物,其籽粒发育所需氮素在很大程度上来源于"源"器官中氮素的再动员,而细胞自噬是植物进行氮素再动员的主要形式之一。为了研究大豆细胞自噬关键基因GmATG8c的功能,本文利用35S启动子驱动GmATG8c基因在大豆中过表达。通过农杆菌介导的大豆子叶节转化法获得了3个独立的单拷贝35S:GmATG8c转基因大豆株系,半定量RT-PCR结果显示,所有转基因株系中目的基因均得到了高效表达。进一步对转基因大豆株系进行的低氮耐受性和产量相关性状分析结果表明,在低氮培养条件下,35S:GmATG8c转基因大豆的生长速率加快,二级分枝数和单株粒数增多,百粒重增加,单株产量显著提高。说明GmATG8c是一个具有提高NUE(nitrogen use effi ciency)育种潜力的基因。展开更多
基金supported by a grant from the National Natural Science Foundation of China (32172552, 31572114)the earmarked fund for the China Agriculture Research System (CARS23-G22)。
文摘To explore the molecular mechanisms by which autophagy contributes to pepper's heat tolerance, we previously identified the zinc-finger protein B-BOX 9/CONSTANS-LIKE 13(CaBBX9/CaCOL13) as an interaction partner of the autophagy related protein(ATG) CaATG8c, a core component in autophagy. However, the involvement of CaBBX9 in both autophagy and heat tolerance remains unclear. In this study, we further confirmed the interaction between CaBBX9 and CaATG8c and defined the interaction regions of CaBBX9 as CONSTANS, CONSTANS-Like, and TOC1(CCT) domain and the fragment region. The expression of CaBBX9 can be induced by heat treatment. CaBBX9 is co-localized with CaATG8c in the nucleus and exhibits a transcriptional activity. When the expression of CaBBX9 is silenced, the heat tolerance of pepper is enhanced, shown by the decrement of MDA content, H2O2and dead cells accumulation, and relative electrolyte leakage, along with the increment of chlorophyll content and expression level of heat-tolerance-related genes. Overexpression of CaBBX9 in tomatoes displays the opposite effects. Taken together, we demonstrate that CaBBX9 negatively regulates the heat tolerance of peppers by exacerbating oxidative damage and inhibiting the expression of heat-related genes. Our findings provide a new clue for guiding crop breeding for pepper tolerance to heat stress.
文摘大豆是一种高需氮素作物,其籽粒发育所需氮素在很大程度上来源于"源"器官中氮素的再动员,而细胞自噬是植物进行氮素再动员的主要形式之一。为了研究大豆细胞自噬关键基因GmATG8c的功能,本文利用35S启动子驱动GmATG8c基因在大豆中过表达。通过农杆菌介导的大豆子叶节转化法获得了3个独立的单拷贝35S:GmATG8c转基因大豆株系,半定量RT-PCR结果显示,所有转基因株系中目的基因均得到了高效表达。进一步对转基因大豆株系进行的低氮耐受性和产量相关性状分析结果表明,在低氮培养条件下,35S:GmATG8c转基因大豆的生长速率加快,二级分枝数和单株粒数增多,百粒重增加,单株产量显著提高。说明GmATG8c是一个具有提高NUE(nitrogen use effi ciency)育种潜力的基因。
