近年来,探究KIFs家族成员在肿瘤发生和发展过程中的功能及作用机制已成为研究热点之一。驱动蛋白超家族包含一类保守的微管依赖性分子运动蛋白,具有腺苷三磷酸酶活性和运动特性。驱动蛋白的主动运动支持多种细胞功能,包括有丝分裂、减...近年来,探究KIFs家族成员在肿瘤发生和发展过程中的功能及作用机制已成为研究热点之一。驱动蛋白超家族包含一类保守的微管依赖性分子运动蛋白,具有腺苷三磷酸酶活性和运动特性。驱动蛋白的主动运动支持多种细胞功能,包括有丝分裂、减数分裂和大分子的转运。有丝分裂是真核细胞分裂的过程,涉及将细胞核、细胞质、细胞器和细胞膜分裂成2个子细胞,这些子细胞成分的部分大致相同。这个过程中的任何错误都可能导致细胞死亡、异常(如基因缺失、染色体易位或重复)和癌症。由于有丝分裂复杂且高度调节,驱动蛋白表达或功能的改变可能导致癌变。此外,由于人类癌症是一种涉及异常细胞生长的基因相关疾病,因此靶向驱动蛋白可能会为控制人类癌症创造一种新的策略。KIF18B属于驱动蛋白家族-8,近年来已经发现部分功能并证明其与多种恶性肿瘤有关。In recent years, investigating the functions and underlying mechanisms of KIFs family members in tumorigenesis and tumor development has emerged as a prominent research area. The kinesin superfamily consists of a group of conserved microtubule-dependent molecular motor proteins, which possess adenosine triphosphatase activity and motility properties. The active motility of kinesins is crucial for supporting diverse cellular functions, such as mitosis, meiosis, and macromolecular transport. Mitosis, the process of eukaryotic cell division, involves the partitioning of the nucleus, cytoplasm, organelles, and cell membrane into two daughter cells with approximately identical components. Any aberration during this process can give rise to cell death, genetic anomalies (e.g., gene deletions, chromosomal translocations, or duplications), and cancer. Given the complexity and highly regulated nature of mitosis, changes in kinesin expression or function may trigger carcinogenesis. Moreover, as human cancer is a gene-related disorder characterized by abnormal cell growth, targeting kinesins could potentially offer a novel strategy for cancer control. KIF18B belongs to the kinesin family-8. In recent years, certain functions of KIF18B have been identified, and it has been demonstrated to be associated with various malignant tumors.展开更多
Neuropeptide Y receptor Y8(NPY8R)is a fish-specific receptor with two subtypes,NPY8AR and NPY8BR.Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest th...Neuropeptide Y receptor Y8(NPY8R)is a fish-specific receptor with two subtypes,NPY8AR and NPY8BR.Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation;this has been found in only a few fish,at present.In order to better understand the physiological function of npy8br,especially in digestion,we used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology to generate npy8br-/-japanese medaka(Oryzias latipes).We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability,ultimately affecting their growth.Specifically,npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes(npy and agrp).npy8br-/-medaka larvae fed for 10 d(10th day of feeding)still had incompletely digested brine shrimp(Artemia nauplii)in the digestive tract 8 h after feeding,the messenger RNA(mRNA)expression levels of digestion-related genes(amy,lpl,ctra,and ctrb)were significantly decreased,and the activity of amylase,trypsin,and lipase also significantly decreased.The deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes(gh and igf1).Hematoxylin and eosin(H&E)sections of intestinal tissue showed that npy8br-/-medaka larvae had damaged intestine,thinned intestinal wall,and shortened intestinal villi.So far,this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.展开更多
基于JESD204B接口协议设计和实现了一种新型8B10B编码器。利用极性信息简化编码码表;利用3B4B与5B6B并行编码提升电路工作频率;利用人为加入一位均衡信息,减少逻辑处理层数。仿真结果表明,电路单元面积1 756 mm2、功耗1.13 m W及最大工...基于JESD204B接口协议设计和实现了一种新型8B10B编码器。利用极性信息简化编码码表;利用3B4B与5B6B并行编码提升电路工作频率;利用人为加入一位均衡信息,减少逻辑处理层数。仿真结果表明,电路单元面积1 756 mm2、功耗1.13 m W及最大工作频率342 m Hz,相较于传统方法具有一定的改进且完全符合JESD204B协议规范。可应用于基于JESD204B接口协议的高速串行接口的设计中。展开更多
文摘近年来,探究KIFs家族成员在肿瘤发生和发展过程中的功能及作用机制已成为研究热点之一。驱动蛋白超家族包含一类保守的微管依赖性分子运动蛋白,具有腺苷三磷酸酶活性和运动特性。驱动蛋白的主动运动支持多种细胞功能,包括有丝分裂、减数分裂和大分子的转运。有丝分裂是真核细胞分裂的过程,涉及将细胞核、细胞质、细胞器和细胞膜分裂成2个子细胞,这些子细胞成分的部分大致相同。这个过程中的任何错误都可能导致细胞死亡、异常(如基因缺失、染色体易位或重复)和癌症。由于有丝分裂复杂且高度调节,驱动蛋白表达或功能的改变可能导致癌变。此外,由于人类癌症是一种涉及异常细胞生长的基因相关疾病,因此靶向驱动蛋白可能会为控制人类癌症创造一种新的策略。KIF18B属于驱动蛋白家族-8,近年来已经发现部分功能并证明其与多种恶性肿瘤有关。In recent years, investigating the functions and underlying mechanisms of KIFs family members in tumorigenesis and tumor development has emerged as a prominent research area. The kinesin superfamily consists of a group of conserved microtubule-dependent molecular motor proteins, which possess adenosine triphosphatase activity and motility properties. The active motility of kinesins is crucial for supporting diverse cellular functions, such as mitosis, meiosis, and macromolecular transport. Mitosis, the process of eukaryotic cell division, involves the partitioning of the nucleus, cytoplasm, organelles, and cell membrane into two daughter cells with approximately identical components. Any aberration during this process can give rise to cell death, genetic anomalies (e.g., gene deletions, chromosomal translocations, or duplications), and cancer. Given the complexity and highly regulated nature of mitosis, changes in kinesin expression or function may trigger carcinogenesis. Moreover, as human cancer is a gene-related disorder characterized by abnormal cell growth, targeting kinesins could potentially offer a novel strategy for cancer control. KIF18B belongs to the kinesin family-8. In recent years, certain functions of KIF18B have been identified, and it has been demonstrated to be associated with various malignant tumors.
基金supported by the National Natural Science Foundation of China(No.31972809)the Key Research&Development Program of Hubei Province(No.2022BBA0051),China.
文摘Neuropeptide Y receptor Y8(NPY8R)is a fish-specific receptor with two subtypes,NPY8AR and NPY8BR.Changes in expression levels during physiological processes or in vivo regulation after ventricular injection suggest that NPY8BR plays an important role in feeding regulation;this has been found in only a few fish,at present.In order to better understand the physiological function of npy8br,especially in digestion,we used clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)technology to generate npy8br-/-japanese medaka(Oryzias latipes).We found that the deletion of npy8br in medaka larvae affected their feeding and digestion ability,ultimately affecting their growth.Specifically,npy8br deficiency in medaka larvae resulted in decreased feed intake and decreased expression levels of orexigenic genes(npy and agrp).npy8br-/-medaka larvae fed for 10 d(10th day of feeding)still had incompletely digested brine shrimp(Artemia nauplii)in the digestive tract 8 h after feeding,the messenger RNA(mRNA)expression levels of digestion-related genes(amy,lpl,ctra,and ctrb)were significantly decreased,and the activity of amylase,trypsin,and lipase also significantly decreased.The deletion of npy8br in medaka larvae inhibited the growth and significantly decreased the expression of growth-related genes(gh and igf1).Hematoxylin and eosin(H&E)sections of intestinal tissue showed that npy8br-/-medaka larvae had damaged intestine,thinned intestinal wall,and shortened intestinal villi.So far,this is the first npy8br gene knockout model established in fish and the first demonstration that npy8br plays an important role in digestion.
文摘基于JESD204B接口协议设计和实现了一种新型8B10B编码器。利用极性信息简化编码码表;利用3B4B与5B6B并行编码提升电路工作频率;利用人为加入一位均衡信息,减少逻辑处理层数。仿真结果表明,电路单元面积1 756 mm2、功耗1.13 m W及最大工作频率342 m Hz,相较于传统方法具有一定的改进且完全符合JESD204B协议规范。可应用于基于JESD204B接口协议的高速串行接口的设计中。
