Objective:To confirm the therapeutic efficacy of the ginkgo biloba extract EGb761 on ischemic stroke and elucidate its underlying mechanism.Methods:Male Sprague-Dawley rats were divided into three groups:sham,model,an...Objective:To confirm the therapeutic efficacy of the ginkgo biloba extract EGb761 on ischemic stroke and elucidate its underlying mechanism.Methods:Male Sprague-Dawley rats were divided into three groups:sham,model,and EGb761(ginkgo biloba extract).Ischemic stroke was then simulated in rats via embolic middle cerebral artery occlusion surgery,with the extract administered half an hour before surgery.Neurological deficit scores,infarct volume,cerebral edema rate,and inflammatory factors served as the primary metrics for drug efficacy.Serum metabolites were analyzed using 1H-nuclear magnetic resonance to elucidate the operative mechanism.Results:Treatment with the ginkgo biloba extract EGb761 significantly ameliorated the neurological deficit scores(P=.0343),diminished the cerebral infarct volume(P=.0001)and cerebral edema rate(P=.0030),and alleviated neuroinflammation(all P<.05)in middle cerebral artery occlusion rats.In addition,it significantly altered the contents of various metabolites,such as 2-hydroxybutyrate,isoleucine,isopropanol,isobutyric acid,N6-acetyllysine,glutamate,glutamine,methionine,and N,Ndimethylglycine(all P<.05).Enrichment analysis of the differential metabolites indicated that EGb761 may be involved in the regulation of amino acid metabolism,betaine metabolism,glucose-alanine cycle,Warburg effect,and urea cycle.Conclusion:The ginkgo biloba extract EGb761 demonstrates anti-ischemic stroke effect on ischemic stroke model rats by regulating amino acids and amino acid derivatives,such as isoleucine,N6-acetyllysine,glutamate,methionine,and N,N-dimethylglycine.展开更多
761S是用Y58S作母本、陆两优996作父本进行杂交,后代不育材料利用人工低温冷灌和海南自然低温对育性进行筛选,经4 a 8代的定向选择培育而成的光温敏核不育系。该不育系具有农艺性状优良、不育起点温度低、柱头外露率高、异交性好、繁殖...761S是用Y58S作母本、陆两优996作父本进行杂交,后代不育材料利用人工低温冷灌和海南自然低温对育性进行筛选,经4 a 8代的定向选择培育而成的光温敏核不育系。该不育系具有农艺性状优良、不育起点温度低、柱头外露率高、异交性好、繁殖产量高及米质优等特性。2023年8月761S及所配组合谷两优99(761S/R2099)均通过湖北省农作物品种审定委员会审定。展开更多
基金supported by the Youth Science Fund Project of the National Natural Science Foundation of China(82104440).
文摘Objective:To confirm the therapeutic efficacy of the ginkgo biloba extract EGb761 on ischemic stroke and elucidate its underlying mechanism.Methods:Male Sprague-Dawley rats were divided into three groups:sham,model,and EGb761(ginkgo biloba extract).Ischemic stroke was then simulated in rats via embolic middle cerebral artery occlusion surgery,with the extract administered half an hour before surgery.Neurological deficit scores,infarct volume,cerebral edema rate,and inflammatory factors served as the primary metrics for drug efficacy.Serum metabolites were analyzed using 1H-nuclear magnetic resonance to elucidate the operative mechanism.Results:Treatment with the ginkgo biloba extract EGb761 significantly ameliorated the neurological deficit scores(P=.0343),diminished the cerebral infarct volume(P=.0001)and cerebral edema rate(P=.0030),and alleviated neuroinflammation(all P<.05)in middle cerebral artery occlusion rats.In addition,it significantly altered the contents of various metabolites,such as 2-hydroxybutyrate,isoleucine,isopropanol,isobutyric acid,N6-acetyllysine,glutamate,glutamine,methionine,and N,Ndimethylglycine(all P<.05).Enrichment analysis of the differential metabolites indicated that EGb761 may be involved in the regulation of amino acid metabolism,betaine metabolism,glucose-alanine cycle,Warburg effect,and urea cycle.Conclusion:The ginkgo biloba extract EGb761 demonstrates anti-ischemic stroke effect on ischemic stroke model rats by regulating amino acids and amino acid derivatives,such as isoleucine,N6-acetyllysine,glutamate,methionine,and N,N-dimethylglycine.
文摘761S是用Y58S作母本、陆两优996作父本进行杂交,后代不育材料利用人工低温冷灌和海南自然低温对育性进行筛选,经4 a 8代的定向选择培育而成的光温敏核不育系。该不育系具有农艺性状优良、不育起点温度低、柱头外露率高、异交性好、繁殖产量高及米质优等特性。2023年8月761S及所配组合谷两优99(761S/R2099)均通过湖北省农作物品种审定委员会审定。
