目的了解鼠疫疫苗株EV76免疫后中国恒河猴血清代谢的改变,为评价其免疫效果提供科学依据。方法将6只恒河猴随机分为2组:EV76免疫组和对照组,每组3只(2雌1雄),免疫组每只动物经腹股沟皮下注射EV761.57×1010CFU,对照组经皮下注射等体...目的了解鼠疫疫苗株EV76免疫后中国恒河猴血清代谢的改变,为评价其免疫效果提供科学依据。方法将6只恒河猴随机分为2组:EV76免疫组和对照组,每组3只(2雌1雄),免疫组每只动物经腹股沟皮下注射EV761.57×1010CFU,对照组经皮下注射等体积PBS。4周后经股静脉采血,采用超高液相色谱-质谱联用技术(ultraperformance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)进行血清非靶向代谢组学检测,按照代谢物的变量重要性投影(variable importance in projection,VIP)>1.0,差异倍数(fold change,FC)>1.5或<0.667,且P<0.05的标准进行差异代谢物筛选,并进行差异代谢物KEGG富集分析。结果共鉴定到正离子模式代谢物460个,负离子模式代谢物440个。经数据库比对,共发现24个差异代谢物;经KEGG通路富集分析,差异代谢物主要涉及甘氨酸、丝氨酸和苏氨酸代谢,苯丙氨酸、酪氨酸和色氨酸的生物合成,缬氨酸、亮氨酸和异亮氨酸的生物合成,苯丙氨酸、半胱氨酸和蛋氨酸、酪氨酸代谢。结论EV76通过增加乙酰左旋肉碱、D-谷氨酰胺、次黄嘌呤核苷、皮质醇等含量,发挥其免疫增强作用,同时存在多种氨基酸代谢通路的异常改变。展开更多
在^(76)Ge同位素生产工艺中,电动调节阀是通过DCS(Distributed Control System)的PID模块来控制,从而实现级联管线内部压力的自动调节;但当工艺系统出现较大压力波动时,现有的电动调节阀控制方法,无法达到最优的控制效果,导致被控工艺...在^(76)Ge同位素生产工艺中,电动调节阀是通过DCS(Distributed Control System)的PID模块来控制,从而实现级联管线内部压力的自动调节;但当工艺系统出现较大压力波动时,现有的电动调节阀控制方法,无法达到最优的控制效果,导致被控工艺压力超出运行安全限值,对工艺系统的安全稳定运行产生了严重不良影响。本研究针对目前电动调节阀在压力异常情况下控制性能较弱问题,提出了对电动调节阀的DCS控制组态程序进行优化的控制方案。实际运行表明,优化后的控制程序避免了异常情况下工艺压力出现较大波动的现象,有力地保障了^(76)Ge同位素生产线的安全平稳运行。展开更多
Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(...Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(CRC).Methods We constructed a protein-protein interaction network of C8orf76-related genes and analyzed the prognostic signatures of C8orf76 and CDK4.Clinicopathological features of C8orf76 and CDK4 were visualized using a nomogram.Results C8orf76 and CDK4 levels were positively correlated in two independent human CRC cohorts(n=83 and n=597).A consistent positive correlation was observed between C8orf76 and CDK4 expression in the CRC cell lines.The nomogram included prognostic genes(C8orf76 and CDK4)and pathological N and M stages.The concordance index(C-index)in our cohort was 0.776,which suggests that the ability of the indicators to predict the overall survival of patients with CRC in our cohort was strong.Conclusion We found that C8orf76 was positively correlated with CDK4 in both the cohorts as well as in CRC cell lines.Therefore,C8orf76 and CDK4 can be used as potential biomarkers to predict the prognosis of CRC.展开更多
文摘目的了解鼠疫疫苗株EV76免疫后中国恒河猴血清代谢的改变,为评价其免疫效果提供科学依据。方法将6只恒河猴随机分为2组:EV76免疫组和对照组,每组3只(2雌1雄),免疫组每只动物经腹股沟皮下注射EV761.57×1010CFU,对照组经皮下注射等体积PBS。4周后经股静脉采血,采用超高液相色谱-质谱联用技术(ultraperformance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)进行血清非靶向代谢组学检测,按照代谢物的变量重要性投影(variable importance in projection,VIP)>1.0,差异倍数(fold change,FC)>1.5或<0.667,且P<0.05的标准进行差异代谢物筛选,并进行差异代谢物KEGG富集分析。结果共鉴定到正离子模式代谢物460个,负离子模式代谢物440个。经数据库比对,共发现24个差异代谢物;经KEGG通路富集分析,差异代谢物主要涉及甘氨酸、丝氨酸和苏氨酸代谢,苯丙氨酸、酪氨酸和色氨酸的生物合成,缬氨酸、亮氨酸和异亮氨酸的生物合成,苯丙氨酸、半胱氨酸和蛋氨酸、酪氨酸代谢。结论EV76通过增加乙酰左旋肉碱、D-谷氨酰胺、次黄嘌呤核苷、皮质醇等含量,发挥其免疫增强作用,同时存在多种氨基酸代谢通路的异常改变。
文摘在^(76)Ge同位素生产工艺中,电动调节阀是通过DCS(Distributed Control System)的PID模块来控制,从而实现级联管线内部压力的自动调节;但当工艺系统出现较大压力波动时,现有的电动调节阀控制方法,无法达到最优的控制效果,导致被控工艺压力超出运行安全限值,对工艺系统的安全稳定运行产生了严重不良影响。本研究针对目前电动调节阀在压力异常情况下控制性能较弱问题,提出了对电动调节阀的DCS控制组态程序进行优化的控制方案。实际运行表明,优化后的控制程序避免了异常情况下工艺压力出现较大波动的现象,有力地保障了^(76)Ge同位素生产线的安全平稳运行。
基金supported by a grant from National Natural Science Foundation of China(No.82203623)2022&2023 Hebei introduction of foreign expert intelligence projects(Nos.YZ202201&YZ202305)+1 种基金Hebei Natural Science Foundation(Nos.H2020206374 and H2021206306)Hebei Clinical Medicine Excellent Talents Project of Province(No.LS202001).
文摘Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(CRC).Methods We constructed a protein-protein interaction network of C8orf76-related genes and analyzed the prognostic signatures of C8orf76 and CDK4.Clinicopathological features of C8orf76 and CDK4 were visualized using a nomogram.Results C8orf76 and CDK4 levels were positively correlated in two independent human CRC cohorts(n=83 and n=597).A consistent positive correlation was observed between C8orf76 and CDK4 expression in the CRC cell lines.The nomogram included prognostic genes(C8orf76 and CDK4)and pathological N and M stages.The concordance index(C-index)in our cohort was 0.776,which suggests that the ability of the indicators to predict the overall survival of patients with CRC in our cohort was strong.Conclusion We found that C8orf76 was positively correlated with CDK4 in both the cohorts as well as in CRC cell lines.Therefore,C8orf76 and CDK4 can be used as potential biomarkers to predict the prognosis of CRC.
