热休克蛋白A6(heat shock 70-kDa protein 6,HSPA6)属于HSP70家族,在哺乳动物谱系中部分保守的可诱导的70-kD蛋白。HSPA6基因位于人类染色体1q23.3上,包含两个重要结构领域:N末端的核苷酸结合域(NBD)和C末端的底物结合域(SBD)。目前研...热休克蛋白A6(heat shock 70-kDa protein 6,HSPA6)属于HSP70家族,在哺乳动物谱系中部分保守的可诱导的70-kD蛋白。HSPA6基因位于人类染色体1q23.3上,包含两个重要结构领域:N末端的核苷酸结合域(NBD)和C末端的底物结合域(SBD)。目前研究发现HSPA6对肿瘤的发生、发展既有抑制作用,也有促进作用,在非肿瘤疾病的罹患中也扮演着多种重要角色。HSPA6已日渐成为研究的热点,但其作用机制仍未明确。本文对HSPA6蛋白的结构、表达、功能及其在肿瘤中的研究进展、可能的作用机制及未来展望作一概述。展开更多
In order to create the Male Sterile Line in tobacco, the anti-sense fragment of HSP70 gene was linked to anther specific expression promoter TA29 and the reconstructed vector was transformed into tobacco by Agrobacter...In order to create the Male Sterile Line in tobacco, the anti-sense fragment of HSP70 gene was linked to anther specific expression promoter TA29 and the reconstructed vector was transformed into tobacco by Agrobacterium mediated transformation, and the transformants were then screened. Gus and spot blotting hybridization analysis of the transformants indicated that anti-sense fragment of HSF70 gene had been integrated into tobacco genome and expressed, thus the male sterile tobacco line was obtained. Microscope observation of anther and pollen showed that pistils of transgenic tobacco were normal, whereas anthers and pollens were fairly abortive in the same transgenic tobacco flower, comparing with pistils and stamens in control plants. The ratio of HSI：＇70 protein before and after heat shock in mitochondrial was found to be 1.39 in control tobacco plants and 1.01 in transgenic tobacco sterile lines. This is suggested that the anti-sense gene fragment of HSP70 can effectively inhibit the expression of HSP70 protein and lead to transgenic male sterility in tobacco flowers. The assay provided a new genetic engineering method for male sterility creation in plants.展开更多
文摘热休克蛋白A6(heat shock 70-kDa protein 6,HSPA6)属于HSP70家族,在哺乳动物谱系中部分保守的可诱导的70-kD蛋白。HSPA6基因位于人类染色体1q23.3上,包含两个重要结构领域:N末端的核苷酸结合域(NBD)和C末端的底物结合域(SBD)。目前研究发现HSPA6对肿瘤的发生、发展既有抑制作用,也有促进作用,在非肿瘤疾病的罹患中也扮演着多种重要角色。HSPA6已日渐成为研究的热点,但其作用机制仍未明确。本文对HSPA6蛋白的结构、表达、功能及其在肿瘤中的研究进展、可能的作用机制及未来展望作一概述。
基金Supported by National Natural Science Foundation of China(30970247)Hunan Provincial Science Fund for Distinguished Young Scholars(11JJ1007)~~
文摘In order to create the Male Sterile Line in tobacco, the anti-sense fragment of HSP70 gene was linked to anther specific expression promoter TA29 and the reconstructed vector was transformed into tobacco by Agrobacterium mediated transformation, and the transformants were then screened. Gus and spot blotting hybridization analysis of the transformants indicated that anti-sense fragment of HSF70 gene had been integrated into tobacco genome and expressed, thus the male sterile tobacco line was obtained. Microscope observation of anther and pollen showed that pistils of transgenic tobacco were normal, whereas anthers and pollens were fairly abortive in the same transgenic tobacco flower, comparing with pistils and stamens in control plants. The ratio of HSI：＇70 protein before and after heat shock in mitochondrial was found to be 1.39 in control tobacco plants and 1.01 in transgenic tobacco sterile lines. This is suggested that the anti-sense gene fragment of HSP70 can effectively inhibit the expression of HSP70 protein and lead to transgenic male sterility in tobacco flowers. The assay provided a new genetic engineering method for male sterility creation in plants.
