Objective To explore the relationship between heat-shock protein 70-2 gene +1267A/G polymorphism(HSP70-2 gene +1267A/G polymorphism) and serum level of high sensitivity C-reactive protein(Hs-CRP) in patients with coro...Objective To explore the relationship between heat-shock protein 70-2 gene +1267A/G polymorphism(HSP70-2 gene +1267A/G polymorphism) and serum level of high sensitivity C-reactive protein(Hs-CRP) in patients with coronary artery disease(CAD).Methods Serum Hs-CRP level and HSP70-2 gene +1267A/G polymorphism were detected in 185 patients with CAD.HSP70-2 gene +1267A/G polymorphism was screened by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results The genotype distribution of the HSP70-2 gene +1267A/G polymorphism was as follows: AA was in 24 of 185 patients(12.97%),AG in 93 of 185 patients(50.27%) and GG in 68 of 185 patients(36.76%).Patients with GG、AG subtype had higher Hs-CRP levels compared with carriers of the AA subtype(4.5±1.8mg/L,3.2±1.2mg/L and 2.7±1.1mg/L,respectively,P<0.05).Among patients who had Hs-CRP >3mg/L,GG,and AG gene subtypes had higher percentage than A allele homozygotes(92.6%,74.2% and 45.8%,respectively.χ2=17.75,28.04,P<0.05).Conclusions This study indicated G allele of HSP70-2 gene +1267A/G polymorphism may be an independent risk factor in systemic inflammation with stable CAD.展开更多
文摘Objective To explore the relationship between heat-shock protein 70-2 gene +1267A/G polymorphism(HSP70-2 gene +1267A/G polymorphism) and serum level of high sensitivity C-reactive protein(Hs-CRP) in patients with coronary artery disease(CAD).Methods Serum Hs-CRP level and HSP70-2 gene +1267A/G polymorphism were detected in 185 patients with CAD.HSP70-2 gene +1267A/G polymorphism was screened by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results The genotype distribution of the HSP70-2 gene +1267A/G polymorphism was as follows: AA was in 24 of 185 patients(12.97%),AG in 93 of 185 patients(50.27%) and GG in 68 of 185 patients(36.76%).Patients with GG、AG subtype had higher Hs-CRP levels compared with carriers of the AA subtype(4.5±1.8mg/L,3.2±1.2mg/L and 2.7±1.1mg/L,respectively,P<0.05).Among patients who had Hs-CRP >3mg/L,GG,and AG gene subtypes had higher percentage than A allele homozygotes(92.6%,74.2% and 45.8%,respectively.χ2=17.75,28.04,P<0.05).Conclusions This study indicated G allele of HSP70-2 gene +1267A/G polymorphism may be an independent risk factor in systemic inflammation with stable CAD.
