This study aimed to investigate inflammatory edema after cerebral ischemia through 7.0T MRI and proton magnetic resonance spectroscopy（MRS）. All SD rats were randomly divided into sham operated group and middle cere...This study aimed to investigate inflammatory edema after cerebral ischemia through 7.0T MRI and proton magnetic resonance spectroscopy（MRS）. All SD rats were randomly divided into sham operated group and middle cerebral artery occlusion（MCAO）-1 day,-3 day and-7 day groups. MRI scan of the brain was performed on a 7.0 Tesla MRI scanner. The volume of positive signals in the ischemic side was detected by using a T2 weighted spinecho multislice sequence; the changes in the height of water-peak were measured with point resolved spectroscopy（PRESS） sequences; cortical edema was detected by using wet-dry weight method; the degrees of nerve injury were evaluated by Bederson neurological score system; double-labeling immunofluorescence technique was used to explore the molecular mechanisms of post-ischemia cerebral edema. The results showed that high T2WI signals were observed in MCAO-1 day,-3 day and-7 day groups, and the water-peak height and water-peak area of MCAO groups were higher than those of sham operated group（P0.05）. Neurological score results were consistent with the degree of brain edema, and a large number of microglia accumulated in the ischemic cortex. Our results suggested that non-invasive MRI technology with the advantage of high spatial resolution and tissue resolution can comprehensively and dynamically observe inflammatory edema after cerebral ischemia from a three-dimensional space, and contribute to evaluation and treatments in clinic.展开更多
目的针对7.0 T磁共振成像(Magnetic Resonance Imaging,MRI)系统在科研临床双模态运行下安全管理的实际需求,构建保障其应用过程中设备、人员、信息等安全的管理体系。方法以西门子7.0 T MRI系统和机房建设为基础,分析整理7.0 T MRI系...目的针对7.0 T磁共振成像(Magnetic Resonance Imaging,MRI)系统在科研临床双模态运行下安全管理的实际需求,构建保障其应用过程中设备、人员、信息等安全的管理体系。方法以西门子7.0 T MRI系统和机房建设为基础,分析整理7.0 T MRI系统双模态应用中存在的安全影响因素,采用失效模式与效应分析法和故障树分析法对以上风险进行评估和分级,组建安全管理团队,建立针对超高强度磁场的安全管理方案,并以此为基准设计7.0 T MRI双模态应用安全管理流程。结果安全管理培训后,工作人员对7.0 T MRI的理论和操作技能显著提高(P<0.001);实施安全管理方案及流程后,7.0 T MRI系统已顺利完成182例检查,多步骤安全筛查流程排除不适合7.0 T MRI检查38人次,发现并去除金属异物26例,保障磁场安全的同时满意率达到98.9%。结论7.0 T MRI系统从纯科研走向临床的转变,为未来无创精准医学奠定了基础,也带来了新的风险和挑战。本研究构建了国内第一个7.0 T MRI双模态应用安全管理体系并实施全面规范的安全管理,期望能为未来7.0 T MRI在国内的进一步推广应用提供安全管理的依据。展开更多
基金supported by the Postdoctoral Science Foundation of China(Nos.201003482,200904-50136)the National Natural Science Foundation of China(No.30900450)
文摘This study aimed to investigate inflammatory edema after cerebral ischemia through 7.0T MRI and proton magnetic resonance spectroscopy（MRS）. All SD rats were randomly divided into sham operated group and middle cerebral artery occlusion（MCAO）-1 day,-3 day and-7 day groups. MRI scan of the brain was performed on a 7.0 Tesla MRI scanner. The volume of positive signals in the ischemic side was detected by using a T2 weighted spinecho multislice sequence; the changes in the height of water-peak were measured with point resolved spectroscopy（PRESS） sequences; cortical edema was detected by using wet-dry weight method; the degrees of nerve injury were evaluated by Bederson neurological score system; double-labeling immunofluorescence technique was used to explore the molecular mechanisms of post-ischemia cerebral edema. The results showed that high T2WI signals were observed in MCAO-1 day,-3 day and-7 day groups, and the water-peak height and water-peak area of MCAO groups were higher than those of sham operated group（P0.05）. Neurological score results were consistent with the degree of brain edema, and a large number of microglia accumulated in the ischemic cortex. Our results suggested that non-invasive MRI technology with the advantage of high spatial resolution and tissue resolution can comprehensively and dynamically observe inflammatory edema after cerebral ischemia from a three-dimensional space, and contribute to evaluation and treatments in clinic.
文摘目的针对7.0 T磁共振成像(Magnetic Resonance Imaging,MRI)系统在科研临床双模态运行下安全管理的实际需求,构建保障其应用过程中设备、人员、信息等安全的管理体系。方法以西门子7.0 T MRI系统和机房建设为基础,分析整理7.0 T MRI系统双模态应用中存在的安全影响因素,采用失效模式与效应分析法和故障树分析法对以上风险进行评估和分级,组建安全管理团队,建立针对超高强度磁场的安全管理方案,并以此为基准设计7.0 T MRI双模态应用安全管理流程。结果安全管理培训后,工作人员对7.0 T MRI的理论和操作技能显著提高(P<0.001);实施安全管理方案及流程后,7.0 T MRI系统已顺利完成182例检查,多步骤安全筛查流程排除不适合7.0 T MRI检查38人次,发现并去除金属异物26例,保障磁场安全的同时满意率达到98.9%。结论7.0 T MRI系统从纯科研走向临床的转变,为未来无创精准医学奠定了基础,也带来了新的风险和挑战。本研究构建了国内第一个7.0 T MRI双模态应用安全管理体系并实施全面规范的安全管理,期望能为未来7.0 T MRI在国内的进一步推广应用提供安全管理的依据。
