Phytochemical studies and antioxidant activities were carried out on n-hexane, ethyl acetate, ethanol and methanol extracts of the leaves of Lippia rugosa A. Chev (Verbenaceae), a medicinal plant used traditionally in...Phytochemical studies and antioxidant activities were carried out on n-hexane, ethyl acetate, ethanol and methanol extracts of the leaves of Lippia rugosa A. Chev (Verbenaceae), a medicinal plant used traditionally in the Cameroonian savannah’s region to protect foodstuffs and to cure degenerative diseases. Results indicated that theses extracts contain terpenoids, phenolic and flavonoid compounds. Except the n-hexane extract, all of the obtained extracts exhibit antioxidant activities with the ethanol extract being the most effective with an inhibition percentage of 85.668% ± 1.233% and an inhibition concentration (IC<sub>50</sub>) of 58 μg/ml (R<sup>2</sup> = 0.987, P < 0.01) at a concentration of 100 mg/ml. Chromatographic separation on silica gel of the ethanol extract led to the isolation of a pure organic compound which was characterized as 7-hydroxy-5,6,4'-trimethox- yflavone by extensive 1D and 2D NMR spectroscopy, a flavonoid exhibiting antioxidative activity with an inhibitory percentage of 25.506% ± 0.205% and inhibition concentration (IC<sub>50</sub>) of 221 μg/ml (R<sup>2</sup> = 0.966, P < 0.01). This is the first time that 7-hydroxy-5,6,4'-trimethoxyflavone is being isolated from L. rugosa and its antioxidant activity evaluated.展开更多
目的:探讨在脂多糖(LPS)诱导的海马神经炎症中,5z-7-氧杂烯醇(OZ)对TAK1/NF-κB信号通路的调控及对新生神经元的保护作用。方法:通过腹腔注射LPS方法制备ICR小鼠神经炎症,治疗组同时腹腔注射OZ。免疫荧光检测海马齿状回Iba-1阳性小胶质...目的:探讨在脂多糖(LPS)诱导的海马神经炎症中,5z-7-氧杂烯醇(OZ)对TAK1/NF-κB信号通路的调控及对新生神经元的保护作用。方法:通过腹腔注射LPS方法制备ICR小鼠神经炎症,治疗组同时腹腔注射OZ。免疫荧光检测海马齿状回Iba-1阳性小胶质细胞的表达和双皮质素(DCX)阳性新生神经元的数量;Western blot检测海马TAK1和NF-κB磷酸化水平和NOX2、COX2、iNOS的表达变化。结果:LPS能够增加Iba-1、p-TAK1和p-NF-κB的表达水平,与对照组比较分别增高1.45±0.12、1.43±0.11和2.07±0.25倍,而OZ处理显著下调上述蛋白的表达水平(P<0.05)。免疫荧光检测发现,LPS组海马齿状回Iba-1阳性区域面积较对照组明显增加(5.38%±0.97%vs 1.89%±1.02%,P<0.05),而OZ处理显著下调Iba-1阳性区域面积(P<0.05)。进一步检测还发现,LPS组齿状回DCX阳性神经元数量较对照组明显减少(51.67±12.10 vs 87.33±11.93),而OZ处理能够增加DCX阳性细胞的数量(P<0.05)。此外,LPS组氧化应激相关蛋白NOX2、COX2和iNOS的表达水平较对照组分别增加1.42±0.09、1.44±0.17和2.74±0.54倍,而OZ处理能够显著下调上述蛋白的表达水平(P<0.05)。结论:OZ能够通过抑制TAK1/NF-κB信号通路,缓解LPS诱导的海马神经炎症,减少新生神经元的损伤。展开更多
The two diarylheptanoids (E)-1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl) hept-4-en-3-one 1 (Gingerenone C) and (±)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(4- hydroxyphenyl)-3-heptanone 2 were synthesized...The two diarylheptanoids (E)-1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl) hept-4-en-3-one 1 (Gingerenone C) and (±)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(4- hydroxyphenyl)-3-heptanone 2 were synthesized from vanillin 3 and 4-hydroxybenzaldehyde 9.展开更多
文摘Phytochemical studies and antioxidant activities were carried out on n-hexane, ethyl acetate, ethanol and methanol extracts of the leaves of Lippia rugosa A. Chev (Verbenaceae), a medicinal plant used traditionally in the Cameroonian savannah’s region to protect foodstuffs and to cure degenerative diseases. Results indicated that theses extracts contain terpenoids, phenolic and flavonoid compounds. Except the n-hexane extract, all of the obtained extracts exhibit antioxidant activities with the ethanol extract being the most effective with an inhibition percentage of 85.668% ± 1.233% and an inhibition concentration (IC<sub>50</sub>) of 58 μg/ml (R<sup>2</sup> = 0.987, P < 0.01) at a concentration of 100 mg/ml. Chromatographic separation on silica gel of the ethanol extract led to the isolation of a pure organic compound which was characterized as 7-hydroxy-5,6,4'-trimethox- yflavone by extensive 1D and 2D NMR spectroscopy, a flavonoid exhibiting antioxidative activity with an inhibitory percentage of 25.506% ± 0.205% and inhibition concentration (IC<sub>50</sub>) of 221 μg/ml (R<sup>2</sup> = 0.966, P < 0.01). This is the first time that 7-hydroxy-5,6,4'-trimethoxyflavone is being isolated from L. rugosa and its antioxidant activity evaluated.
文摘目的:探讨在脂多糖(LPS)诱导的海马神经炎症中,5z-7-氧杂烯醇(OZ)对TAK1/NF-κB信号通路的调控及对新生神经元的保护作用。方法:通过腹腔注射LPS方法制备ICR小鼠神经炎症,治疗组同时腹腔注射OZ。免疫荧光检测海马齿状回Iba-1阳性小胶质细胞的表达和双皮质素(DCX)阳性新生神经元的数量;Western blot检测海马TAK1和NF-κB磷酸化水平和NOX2、COX2、iNOS的表达变化。结果:LPS能够增加Iba-1、p-TAK1和p-NF-κB的表达水平,与对照组比较分别增高1.45±0.12、1.43±0.11和2.07±0.25倍,而OZ处理显著下调上述蛋白的表达水平(P<0.05)。免疫荧光检测发现,LPS组海马齿状回Iba-1阳性区域面积较对照组明显增加(5.38%±0.97%vs 1.89%±1.02%,P<0.05),而OZ处理显著下调Iba-1阳性区域面积(P<0.05)。进一步检测还发现,LPS组齿状回DCX阳性神经元数量较对照组明显减少(51.67±12.10 vs 87.33±11.93),而OZ处理能够增加DCX阳性细胞的数量(P<0.05)。此外,LPS组氧化应激相关蛋白NOX2、COX2和iNOS的表达水平较对照组分别增加1.42±0.09、1.44±0.17和2.74±0.54倍,而OZ处理能够显著下调上述蛋白的表达水平(P<0.05)。结论:OZ能够通过抑制TAK1/NF-κB信号通路,缓解LPS诱导的海马神经炎症,减少新生神经元的损伤。
文摘The two diarylheptanoids (E)-1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl) hept-4-en-3-one 1 (Gingerenone C) and (±)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(4- hydroxyphenyl)-3-heptanone 2 were synthesized from vanillin 3 and 4-hydroxybenzaldehyde 9.
