鱼类的性腺分化与配子发生受到多种因子的调控。蛋白磷酸酶(protein phosphatase,PP)具有催化生物体中蛋白去磷酸化的功能。蛋白磷酸酶6(protein phosphatase type 6,PP6)作为蛋白磷酸酶家族的一员,在性腺发育及配子发生过程中发挥重要...鱼类的性腺分化与配子发生受到多种因子的调控。蛋白磷酸酶(protein phosphatase,PP)具有催化生物体中蛋白去磷酸化的功能。蛋白磷酸酶6(protein phosphatase type 6,PP6)作为蛋白磷酸酶家族的一员,在性腺发育及配子发生过程中发挥重要功能。ppp6r3编码蛋白磷酸酶6调节亚基3,其在性腺分化和配子发生方面的作用还不清楚。本研究通过CRISPR/Cas9技术构建了两个斑马鱼ppp6r3突变品系,发现ppp6r3缺失后导致斑马鱼仅发育为雄鱼,且该突变体不能使野生型卵子受精。进一步研究发现,ppp6r3突变体精巢中精母细胞向成熟精子转换失败,精子发生受阻。综上所述,本研究构建了ppp6r3突变的斑马鱼模型,表现出性腺分化和配子发生的缺陷,为后续研究Ppp6r3调控生殖细胞增殖、分化及性别决定的机制提供了模型和参考。展开更多
Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared wit...Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared with the resistance genes from common wheat,alien resistance genes can better withstand infection of this highly variable pathogen.Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding.In this study,three wheat-rye germplasm lines YT4-1,YT4-2,and YT4-3 were developed through hybridization between octoploid triticale and common wheat,out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance(APR)to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages.Using genomic in situ hybridization,multi-color fluorescence in situ hybridization,multi-color GISH,and molecular marker analysis,YT4-1,YT4-2,and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS.1BL translocation line,6RL ditelosomic addition and T1RS.1BL translocation line,and T1RS.1BL translocation line,respectively.Compared with previously reported wheat-rye derivative lines carrying chromosome 6R,YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits.Therefore,these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding.展开更多
为了提高工业机器人的交互性,进行了构建具有三维可视化环境的人机交互系统研究。本文以6R机器人为对象建立了机器人运动学模型,利用矢量积方法推导了雅克比矩阵,为仿真和监控数据的可视化奠定了基础。给出了可视化环境的实现方案,设计...为了提高工业机器人的交互性,进行了构建具有三维可视化环境的人机交互系统研究。本文以6R机器人为对象建立了机器人运动学模型,利用矢量积方法推导了雅克比矩阵,为仿真和监控数据的可视化奠定了基础。给出了可视化环境的实现方案,设计了编译控制指令和仿真的算法流程,并以JOpen Show Var作为网络通信开发包设计了监控的通信结构。开发实现了机器人的仿真和监控系统,并进行了实验验证。实验结果表明该系统具有形象直观的人机交互环境,而且具有良好的监控实时性。展开更多
文摘鱼类的性腺分化与配子发生受到多种因子的调控。蛋白磷酸酶(protein phosphatase,PP)具有催化生物体中蛋白去磷酸化的功能。蛋白磷酸酶6(protein phosphatase type 6,PP6)作为蛋白磷酸酶家族的一员,在性腺发育及配子发生过程中发挥重要功能。ppp6r3编码蛋白磷酸酶6调节亚基3,其在性腺分化和配子发生方面的作用还不清楚。本研究通过CRISPR/Cas9技术构建了两个斑马鱼ppp6r3突变品系,发现ppp6r3缺失后导致斑马鱼仅发育为雄鱼,且该突变体不能使野生型卵子受精。进一步研究发现,ppp6r3突变体精巢中精母细胞向成熟精子转换失败,精子发生受阻。综上所述,本研究构建了ppp6r3突变的斑马鱼模型,表现出性腺分化和配子发生的缺陷,为后续研究Ppp6r3调控生殖细胞增殖、分化及性别决定的机制提供了模型和参考。
基金This research was supported by the National Key Research and Development Program of China(2021YFD1200600)the National Natural Science Foundation of China(32272105).
文摘Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is a devastating disease that seriously threatens wheat yield and quality.To control this disease,host resistance is the most effective measure.Compared with the resistance genes from common wheat,alien resistance genes can better withstand infection of this highly variable pathogen.Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding.In this study,three wheat-rye germplasm lines YT4-1,YT4-2,and YT4-3 were developed through hybridization between octoploid triticale and common wheat,out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance(APR)to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages.Using genomic in situ hybridization,multi-color fluorescence in situ hybridization,multi-color GISH,and molecular marker analysis,YT4-1,YT4-2,and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS.1BL translocation line,6RL ditelosomic addition and T1RS.1BL translocation line,and T1RS.1BL translocation line,respectively.Compared with previously reported wheat-rye derivative lines carrying chromosome 6R,YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits.Therefore,these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding.
文摘为了提高工业机器人的交互性,进行了构建具有三维可视化环境的人机交互系统研究。本文以6R机器人为对象建立了机器人运动学模型,利用矢量积方法推导了雅克比矩阵,为仿真和监控数据的可视化奠定了基础。给出了可视化环境的实现方案,设计了编译控制指令和仿真的算法流程,并以JOpen Show Var作为网络通信开发包设计了监控的通信结构。开发实现了机器人的仿真和监控系统,并进行了实验验证。实验结果表明该系统具有形象直观的人机交互环境,而且具有良好的监控实时性。
