鱼类的性腺分化与配子发生受到多种因子的调控。蛋白磷酸酶(protein phosphatase,PP)具有催化生物体中蛋白去磷酸化的功能。蛋白磷酸酶6(protein phosphatase type 6,PP6)作为蛋白磷酸酶家族的一员,在性腺发育及配子发生过程中发挥重要...鱼类的性腺分化与配子发生受到多种因子的调控。蛋白磷酸酶(protein phosphatase,PP)具有催化生物体中蛋白去磷酸化的功能。蛋白磷酸酶6(protein phosphatase type 6,PP6)作为蛋白磷酸酶家族的一员,在性腺发育及配子发生过程中发挥重要功能。ppp6r3编码蛋白磷酸酶6调节亚基3,其在性腺分化和配子发生方面的作用还不清楚。本研究通过CRISPR/Cas9技术构建了两个斑马鱼ppp6r3突变品系,发现ppp6r3缺失后导致斑马鱼仅发育为雄鱼,且该突变体不能使野生型卵子受精。进一步研究发现,ppp6r3突变体精巢中精母细胞向成熟精子转换失败,精子发生受阻。综上所述,本研究构建了ppp6r3突变的斑马鱼模型,表现出性腺分化和配子发生的缺陷,为后续研究Ppp6r3调控生殖细胞增殖、分化及性别决定的机制提供了模型和参考。展开更多
To investigate the effect of structural variation of rye 6RL arms on their meiotic behavior and to locate powdery mildew resistance gene(s),we developed a wheat–rye T6BS.6RL^(Ar)translocation chromosome and its delet...To investigate the effect of structural variation of rye 6RL arms on their meiotic behavior and to locate powdery mildew resistance gene(s),we developed a wheat–rye T6BS.6RL^(Ar)translocation chromosome and its deleted translocations,T6BS.6RL^(Ar)-2 and T6BS.6RL^(Ar)-4.Some 6RL-specific markers were used to determine that the segments from 733.91 to 849.52 Mb and from 832.72 Mb to the telomere of 6RL^(Ar)arms were deleted from T6BS.6RL^(Ar)-2 and T6BS.6RL^(Ar)-4,respectively.Translocations T6BS.6RL^(Ar)and T6BS.6RL^(Ar)-4 were resistant to powdery mildew and T6BS.6RL^(Ar)-2 was susceptible.The segment of 6RL^(Ar)with powdery mildew resistance was about 100 Mb.Deletion of the 6RL^(Ar)telomeric region inhibited the pairing and recombination of T6BS.6RL^(Ar)-4.Compared with T6BS.6RL^(Ar)and T6BS.6RL^(Ar)-4,T6BS.6RL^(Ar)-2 showed a normal meiotic behavior.Immunolocalization using anti-ZYP1,anti-DMC1 and anti-MLH1 proteins indicated that more DSBs(DNA double-strand breaks)and crossovers formed on the 6RL^(Ar)arm in T6BS.6RL^(Ar),and this might be related to the formation of anaphase I bridges of 6RL^(Ar).Although the 6RL^(Ar)deletions were used to physically locate powdery mildew resistance gene(s),more accurate location through meiotic recombination was needed.The results in this study indicated that altering the structure of the 6RL^(Ar)arm promoted normal meiotic behavior,and this might facilitate the localization of resistance genes through meiotic homologous recombination.展开更多
为了提高工业机器人的交互性,进行了构建具有三维可视化环境的人机交互系统研究。本文以6R机器人为对象建立了机器人运动学模型,利用矢量积方法推导了雅克比矩阵,为仿真和监控数据的可视化奠定了基础。给出了可视化环境的实现方案,设计...为了提高工业机器人的交互性,进行了构建具有三维可视化环境的人机交互系统研究。本文以6R机器人为对象建立了机器人运动学模型,利用矢量积方法推导了雅克比矩阵,为仿真和监控数据的可视化奠定了基础。给出了可视化环境的实现方案,设计了编译控制指令和仿真的算法流程,并以JOpen Show Var作为网络通信开发包设计了监控的通信结构。开发实现了机器人的仿真和监控系统,并进行了实验验证。实验结果表明该系统具有形象直观的人机交互环境,而且具有良好的监控实时性。展开更多
文摘鱼类的性腺分化与配子发生受到多种因子的调控。蛋白磷酸酶(protein phosphatase,PP)具有催化生物体中蛋白去磷酸化的功能。蛋白磷酸酶6(protein phosphatase type 6,PP6)作为蛋白磷酸酶家族的一员,在性腺发育及配子发生过程中发挥重要功能。ppp6r3编码蛋白磷酸酶6调节亚基3,其在性腺分化和配子发生方面的作用还不清楚。本研究通过CRISPR/Cas9技术构建了两个斑马鱼ppp6r3突变品系,发现ppp6r3缺失后导致斑马鱼仅发育为雄鱼,且该突变体不能使野生型卵子受精。进一步研究发现,ppp6r3突变体精巢中精母细胞向成熟精子转换失败,精子发生受阻。综上所述,本研究构建了ppp6r3突变的斑马鱼模型,表现出性腺分化和配子发生的缺陷,为后续研究Ppp6r3调控生殖细胞增殖、分化及性别决定的机制提供了模型和参考。
基金supported by the Sichuan Science and Technology Program(2022ZDZX0014)the National Natural Science Foundation of China(31770373).
文摘To investigate the effect of structural variation of rye 6RL arms on their meiotic behavior and to locate powdery mildew resistance gene(s),we developed a wheat–rye T6BS.6RL^(Ar)translocation chromosome and its deleted translocations,T6BS.6RL^(Ar)-2 and T6BS.6RL^(Ar)-4.Some 6RL-specific markers were used to determine that the segments from 733.91 to 849.52 Mb and from 832.72 Mb to the telomere of 6RL^(Ar)arms were deleted from T6BS.6RL^(Ar)-2 and T6BS.6RL^(Ar)-4,respectively.Translocations T6BS.6RL^(Ar)and T6BS.6RL^(Ar)-4 were resistant to powdery mildew and T6BS.6RL^(Ar)-2 was susceptible.The segment of 6RL^(Ar)with powdery mildew resistance was about 100 Mb.Deletion of the 6RL^(Ar)telomeric region inhibited the pairing and recombination of T6BS.6RL^(Ar)-4.Compared with T6BS.6RL^(Ar)and T6BS.6RL^(Ar)-4,T6BS.6RL^(Ar)-2 showed a normal meiotic behavior.Immunolocalization using anti-ZYP1,anti-DMC1 and anti-MLH1 proteins indicated that more DSBs(DNA double-strand breaks)and crossovers formed on the 6RL^(Ar)arm in T6BS.6RL^(Ar),and this might be related to the formation of anaphase I bridges of 6RL^(Ar).Although the 6RL^(Ar)deletions were used to physically locate powdery mildew resistance gene(s),more accurate location through meiotic recombination was needed.The results in this study indicated that altering the structure of the 6RL^(Ar)arm promoted normal meiotic behavior,and this might facilitate the localization of resistance genes through meiotic homologous recombination.
文摘为了提高工业机器人的交互性,进行了构建具有三维可视化环境的人机交互系统研究。本文以6R机器人为对象建立了机器人运动学模型,利用矢量积方法推导了雅克比矩阵,为仿真和监控数据的可视化奠定了基础。给出了可视化环境的实现方案,设计了编译控制指令和仿真的算法流程,并以JOpen Show Var作为网络通信开发包设计了监控的通信结构。开发实现了机器人的仿真和监控系统,并进行了实验验证。实验结果表明该系统具有形象直观的人机交互环境,而且具有良好的监控实时性。
