Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offs...Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offspring from mating B6C3Fe Sox10 ^(Dom/+)male mice with C57BL/6J female mice,and name the progeny B6C3Fe-g.Further,conduct separate self-breeding for both the B6C3Fe and B6C3Fe-g strains,adhering to the principle of pairing mutants with non-mutants.By comparing the number of offspring,survival rates,and the phenotype of aganglionosis in the colon,a comprehensive evaluation of their breeding capacity and phenotypic stability is conducted.Results:Sanger sequencing results show that the mutation sites of B6C3Fe and B6C3Fe-g mice are consistent.After fluorescent staining of intestinal nerves,it was found that the heterozygous mice of the two strains had neuronal deletion in the distal colon,and this pathological phenotype was consistent with the pathological features of the diseased colon of Hirschsprung disease(HSCR).However,compared with the B6C3Fe strain,the B6C3Fe-g strain has a higher number of offspring and greater survival rates.Conclusions:The breeding strategy of the B6C3Fe-g strain ensures genetic and phenotypic stability,while improving reproductive efficiency,and is an ideal scheme for breeding Sox10 ^(Dom/+)mice.展开更多
基金Basic and Applied Basic Research Foundation of Guangxi Province of China,Grant/Award Number:2025GXNSFBA069072National Natural Science Foundation of China,Grant/Award Number:81970450,82070528,82170528,82200561,82201893,82301955,82370526 and 82560108+2 种基金Medical Scientific Research Foundation of Guangdong Province of China,Grant/Award Number:A2024374The Guangdong Basic and Applied Basic Research Foundation,Grant/Award Number:2024A03J1171,2024A03J1238 and 2024A1515013190Science and Technology Projects in Guangzhou,Grant/Award Number:202201020006。
文摘Background:The traditional Sox10 ^(Dom/+)mouse breeding strategy is costly and timeconsuming,so this study aims to optimize the breeding method and improve the scientific research efficiency.Methods:We select the offspring from mating B6C3Fe Sox10 ^(Dom/+)male mice with C57BL/6J female mice,and name the progeny B6C3Fe-g.Further,conduct separate self-breeding for both the B6C3Fe and B6C3Fe-g strains,adhering to the principle of pairing mutants with non-mutants.By comparing the number of offspring,survival rates,and the phenotype of aganglionosis in the colon,a comprehensive evaluation of their breeding capacity and phenotypic stability is conducted.Results:Sanger sequencing results show that the mutation sites of B6C3Fe and B6C3Fe-g mice are consistent.After fluorescent staining of intestinal nerves,it was found that the heterozygous mice of the two strains had neuronal deletion in the distal colon,and this pathological phenotype was consistent with the pathological features of the diseased colon of Hirschsprung disease(HSCR).However,compared with the B6C3Fe strain,the B6C3Fe-g strain has a higher number of offspring and greater survival rates.Conclusions:The breeding strategy of the B6C3Fe-g strain ensures genetic and phenotypic stability,while improving reproductive efficiency,and is an ideal scheme for breeding Sox10 ^(Dom/+)mice.
