热休克蛋白A6(heat shock 70-kDa protein 6,HSPA6)属于HSP70家族,在哺乳动物谱系中部分保守的可诱导的70-kD蛋白。HSPA6基因位于人类染色体1q23.3上,包含两个重要结构领域:N末端的核苷酸结合域(NBD)和C末端的底物结合域(SBD)。目前研...热休克蛋白A6(heat shock 70-kDa protein 6,HSPA6)属于HSP70家族,在哺乳动物谱系中部分保守的可诱导的70-kD蛋白。HSPA6基因位于人类染色体1q23.3上,包含两个重要结构领域:N末端的核苷酸结合域(NBD)和C末端的底物结合域(SBD)。目前研究发现HSPA6对肿瘤的发生、发展既有抑制作用,也有促进作用,在非肿瘤疾病的罹患中也扮演着多种重要角色。HSPA6已日渐成为研究的热点,但其作用机制仍未明确。本文对HSPA6蛋白的结构、表达、功能及其在肿瘤中的研究进展、可能的作用机制及未来展望作一概述。展开更多
目的探讨核糖体蛋白激酶A6(RSK4)在甲状腺乳头状癌(PTC)中的表达及其与临床病理因素的关系。方法 收集200例PTC(A组)和对应的癌旁组织(B组),40例甲状腺良性结节(C组)及其结节旁组织(D组)新鲜标本,提取相应RNA,并逆转录成cDNA,采用实时...目的探讨核糖体蛋白激酶A6(RSK4)在甲状腺乳头状癌(PTC)中的表达及其与临床病理因素的关系。方法 收集200例PTC(A组)和对应的癌旁组织(B组),40例甲状腺良性结节(C组)及其结节旁组织(D组)新鲜标本,提取相应RNA,并逆转录成cDNA,采用实时荧光定量PCR(RT-qPCR)检测RSK4的表达量,并分析其与PTC患者临床病理因素的关系。结果 A组中RSK4表达量显著低于B组( Z =-9.658, P <0.01)和C组( Z =-5.648, P <0.01),但C组RSK4表达量与D组比较差异无显著性( P >0.05)。肿瘤直径>1 cm的PTC组织中RSK4表达量显著低于≤1 cm组( Z =-3.528, P <0.01),有包膜侵犯的PTC组织中RSK4表达量显著低于无包膜侵犯组( Z =-2.822, P <0.01)。结论 RSK4在PTC组织中低表达,且与肿瘤大小、包膜侵犯相关,可能成为PTC诊断和预后评估的潜在生物标志物。展开更多
Objective:There are limited data on the use of the creation tuberculin skin test(C-TST)for detecting tuberculosis(TB)infection(TBI)in individuals under 18 years of age.We conducted a study to assess the diagnostic acc...Objective:There are limited data on the use of the creation tuberculin skin test(C-TST)for detecting tuberculosis(TB)infection(TBI)in individuals under 18 years of age.We conducted a study to assess the diagnostic accuracy of C-TST in this population.Methods:A double-blind,randomized controlled trial was conducted across 4 tertiary hospitals in China to evaluate the diagnostic accuracy of the C-TST in detecting TBI in individuals under 18 years of age.Participants with suspected pulmonary TB,extrapulmonary TB,or non-TB pulmonary disease were enrolled.The primary outcome was the diagnostic accuracy of the C-TST.Secondary outcomes included the consistency among C-TST,the traditional tuberculin skin test(TST),and T-SPOT.TB assays in different subgroups,as well as the safety of C-TST.Each participant underwent all 3 tests simultaneously:T-SPOT.TB assay,TST,and C-TST.Results:C-TST showed a sensitivity of 83.0%(95%CI,68.7%-91.9%),while TST and T-SPOT.TB demonstrated sensitivities of 80.9%(95%CI,66.3%-90.4%)and 76.6%(95%CI,61.6%-87.2%),respectively.The specificities of C-TST,TST,and T-SPOT.TB were 100%(95%CI,91.9%-100%),98.0%(95%CI,87.8%-99.9%),and 100%(95%CI,90.9%-100%),respectively.The consistency between C-TST and T-SPOT.TB was high(kappa=0.847).No serious adverse events(AEs)were reported.展开更多
文摘热休克蛋白A6(heat shock 70-kDa protein 6,HSPA6)属于HSP70家族,在哺乳动物谱系中部分保守的可诱导的70-kD蛋白。HSPA6基因位于人类染色体1q23.3上,包含两个重要结构领域:N末端的核苷酸结合域(NBD)和C末端的底物结合域(SBD)。目前研究发现HSPA6对肿瘤的发生、发展既有抑制作用,也有促进作用,在非肿瘤疾病的罹患中也扮演着多种重要角色。HSPA6已日渐成为研究的热点,但其作用机制仍未明确。本文对HSPA6蛋白的结构、表达、功能及其在肿瘤中的研究进展、可能的作用机制及未来展望作一概述。
文摘目的探讨核糖体蛋白激酶A6(RSK4)在甲状腺乳头状癌(PTC)中的表达及其与临床病理因素的关系。方法 收集200例PTC(A组)和对应的癌旁组织(B组),40例甲状腺良性结节(C组)及其结节旁组织(D组)新鲜标本,提取相应RNA,并逆转录成cDNA,采用实时荧光定量PCR(RT-qPCR)检测RSK4的表达量,并分析其与PTC患者临床病理因素的关系。结果 A组中RSK4表达量显著低于B组( Z =-9.658, P <0.01)和C组( Z =-5.648, P <0.01),但C组RSK4表达量与D组比较差异无显著性( P >0.05)。肿瘤直径>1 cm的PTC组织中RSK4表达量显著低于≤1 cm组( Z =-3.528, P <0.01),有包膜侵犯的PTC组织中RSK4表达量显著低于无包膜侵犯组( Z =-2.822, P <0.01)。结论 RSK4在PTC组织中低表达,且与肿瘤大小、包膜侵犯相关,可能成为PTC诊断和预后评估的潜在生物标志物。
基金supported by Anhui Longcom Biologic Pharmacy Co.Ltd.,ChinaThis study received grant support from the Clinical Research Project of Shanghai Public Health Clinical Center of China(No.KY-GW-2024-01)research start-up funds for introduced talents of Shanghai Public Health Clinical Center of China(No.RCJJ2025-08).
文摘Objective:There are limited data on the use of the creation tuberculin skin test(C-TST)for detecting tuberculosis(TB)infection(TBI)in individuals under 18 years of age.We conducted a study to assess the diagnostic accuracy of C-TST in this population.Methods:A double-blind,randomized controlled trial was conducted across 4 tertiary hospitals in China to evaluate the diagnostic accuracy of the C-TST in detecting TBI in individuals under 18 years of age.Participants with suspected pulmonary TB,extrapulmonary TB,or non-TB pulmonary disease were enrolled.The primary outcome was the diagnostic accuracy of the C-TST.Secondary outcomes included the consistency among C-TST,the traditional tuberculin skin test(TST),and T-SPOT.TB assays in different subgroups,as well as the safety of C-TST.Each participant underwent all 3 tests simultaneously:T-SPOT.TB assay,TST,and C-TST.Results:C-TST showed a sensitivity of 83.0%(95%CI,68.7%-91.9%),while TST and T-SPOT.TB demonstrated sensitivities of 80.9%(95%CI,66.3%-90.4%)and 76.6%(95%CI,61.6%-87.2%),respectively.The specificities of C-TST,TST,and T-SPOT.TB were 100%(95%CI,91.9%-100%),98.0%(95%CI,87.8%-99.9%),and 100%(95%CI,90.9%-100%),respectively.The consistency between C-TST and T-SPOT.TB was high(kappa=0.847).No serious adverse events(AEs)were reported.
