The methods of determination of 6-methyl coumarin in cosmetics by HPLC-DAD and GC-FID were compared. The positive samples were confirmed by GC-MS. Methanol was used to extract 6-methyl coumarin from cosmetics. The chr...The methods of determination of 6-methyl coumarin in cosmetics by HPLC-DAD and GC-FID were compared. The positive samples were confirmed by GC-MS. Methanol was used to extract 6-methyl coumarin from cosmetics. The chromatographic conditions of the two methods were optimized and the samples were determined. The recovery, precision, correlation coefficient, detection limit and quantitative limit of the two methods were compared. The results showed that the linear relationship between the two methods was good. The percentage recovery of standard addition by HPLC-DAD was 92.20%~102.80%, by RSD was 0.77%~2.00%;the detection limit was 0.05 mg/kg, the quantitative limit was 0.17 mg/kg. The percentage recovery of standard addition by GC-FID was 97.30%~103.28%, by RSD was 0.47%~4.87%;the detection limit was 1.3 mg/kg, the quantitative limit was 5.0 mg/kg. The detection limit by HPLC-DAD was lower and more sensitive than that by GC-FID , which also shows that HPLC-DAD has more advantages for the detection of low content samples.展开更多
Objective To observe the clinical effect of acupuncture at Neiguan （内关 PC 6） in combination with respiratory reinforcing and reducing method for treatment of cardiac neurosis. Methods Thirty-eight patients with ca...Objective To observe the clinical effect of acupuncture at Neiguan （内关 PC 6） in combination with respiratory reinforcing and reducing method for treatment of cardiac neurosis. Methods Thirty-eight patients with cardiac neurosis were selected for treatment through acupuncture at PC 6 in combination with respiratory reinforcing and reducing method. With twice per day, treated for 6 days per week, and 4 weeks as one course, the therapeutic effect was assessed after one course. Results Twenty-one cases were cured, 15 cases were remarkably improved, and 2 cases were failed. The total effective rate was 94.7%. Conclusion Acupuncture at PC 6 in combination with respiratory reinforcing and reducing method is effective in treatment of cardiac neurosis.展开更多
In this study,a high-throughput screening method was established through the 24-square deep-well microliter plate(MTP) fermentation and micro-plate detection for large-scale screening of the mutants.It was suitable fo...In this study,a high-throughput screening method was established through the 24-square deep-well microliter plate(MTP) fermentation and micro-plate detection for large-scale screening of the mutants.It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation.Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation.The filter paper activity and β-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23%separately compared with A.niger H11 by shaking flask fermentation,and it was genetically stable after being passaged to nine generations.The results indicate that the high-throughput screening method can be used for the quick breeding of A.niger with high cellulase activity.展开更多
The structure parameters of 6-degree of freedom(DOF)vibration isolation platform have a significant effect on its performance.To make the designed vibration isolation platform perform well,non-dominanted sorting genet...The structure parameters of 6-degree of freedom(DOF)vibration isolation platform have a significant effect on its performance.To make the designed vibration isolation platform perform well,non-dominanted sorting genetic algorithm version II(NSGA-II)was applied to optimize its structure based on the transfer matrix method for multibody systems.Firstly,the Jacobian matrix of 6-DOF vibration isolation platform was solved based on kinematics.Secondly,the transfer equation of 6-DOF vibration isolation system was established by the linear transfer matrix method for multibody systems.And the formula of its natural frequency was derived according to the boundary conditions of the system.Thirdly,the manipulability index was constructed based on a dimensionless Jacobian matrix.And a new performance index function was established considering the influence of dynamic isotropic and legs mass.Fourthly,genetic algorithm(GA)and NSGA-II were used to optimize the structure of the 6-DOF vibration isolation platform under the same conditions,respectively.It showed that NSGA-II had higher optimization efficiency,better calculation accuracy and shorter optimization time than that of GA.Finally,NSGA-II was adopted for multi-objective optimization design of 6-DOF vibration isolation platform based on the constraint conditions.Optimal Pareto solutions were obtained,which provides structural parameters for subsequent design work.Therefore,the proposed optimization method and the performance index in this paper provide a theoretical basis for the optimal design of relevant vibration isolation mechanism.展开更多
Ti6Al4V substrates were anodized in a 0.5 mol/L H_2SO_4 solution at applied voltages of 90-140 V.A hydroxyapatite-titanium oxide（HA-TiO2）coating was then deposited on the anodized Ti6Al4 V substrates via a hydrother...Ti6Al4V substrates were anodized in a 0.5 mol/L H_2SO_4 solution at applied voltages of 90-140 V.A hydroxyapatite-titanium oxide（HA-TiO2）coating was then deposited on the anodized Ti6Al4 V substrates via a hydrothermal-electrochemicalmethod at a constant current.The obtained films and coatings were characterized by X-ray diffraction,scanning electron microscopy,energy-dispersive X-ray spectroscopy,and Fourier-transform infrared spectrometry.The microstructures of the porous films on the Ti6Al4 V substrates were studied to investigate the effect of the anodizing voltage on the phase and morphology of the HATiO_2 coating.The results indicated that both the phase composition and the morphology of the coatings were significantly influenced by changes in the anodizing voltage.HA-TiO_2 was directly precipitated onto the surface of the substrate when the applied voltage was between 110 and 140 V.The coatings had a gradient structure and the HA exhibited both needle-like and cotton-like structures.The amount of cotton-like HA structures decreased with an increase in voltage from 90 to 120 V,and then increased slightly when the voltage was higher than 120 V.The orientation index of the（002）plane of the coating was at a minimum when the Ti6Al4 V substrate was pretreated at 120 V.展开更多
In this work, ZnSn（OH）6with a cubic structure is successfully synthesized by one-step hydrothermal method without any catalyst. The response and recovery characteristics of gas sensing were investigated against vari...In this work, ZnSn（OH）6with a cubic structure is successfully synthesized by one-step hydrothermal method without any catalyst. The response and recovery characteristics of gas sensing were investigated against various gases via quartz crystal microbalance（QCM） at room temperature. The sensor exhibited high sensitivity and good selectivity toward CO gas. Moreover, a linear dependence of log^（àDelta F）about CO concentration was obtained. It is demonstrated that the QCM sensor coated cubic ZnSn（OH）6could be a suitable candidate for detecting CO.展开更多
Pr0.6-x NdxCa0.4 FeO3-δ （ x = 0.0, 0.2, 0.3, 0.4, 0.5, 0.6） were synthesized using Pechini method. A number of studies were conducted concerning composition, specific area, crystalline structure and microstructure ...Pr0.6-x NdxCa0.4 FeO3-δ （ x = 0.0, 0.2, 0.3, 0.4, 0.5, 0.6） were synthesized using Pechini method. A number of studies were conducted concerning composition, specific area, crystalline structure and microstructure of the samples by means of FT-IR, BET, XRD TG-DTA and SEM. The results show that all the samples with different doping amounts of Pr^3＋ and Nd^3＋ on A-site are fine dispersed, and mean particle size less than 100 nm. The powders have good sinterability, and the relative density is 95% after sintered at 1200 ℃ for 2 h. It is found that all specimens are entirely single phase solid solutions with orthorhombic perovskite structure, the stable perovskitetype phase is formed completely after calcination at 900 ℃.展开更多
In this paper, we evaluate semiempirical methods (AM1, PM3, and ZINDO), HF and DFT (B3LYP) in different basis sets to determine which method best describes the sign and magnitude of the geometrical parameters of artem...In this paper, we evaluate semiempirical methods (AM1, PM3, and ZINDO), HF and DFT (B3LYP) in different basis sets to determine which method best describes the sign and magnitude of the geometrical parameters of artemisinin in the region of the endoperoxide ring compared to crystallographic data. We also classify these methods using statistical analysis. The results of PCA were based on three main components, explaining 98.0539% of the total variance, for the geometrical parameters C3O13, O1O2C3, O13C12C12a, and O2C3O13C12. The DFT method (B3LYP) corresponded well with the experimental data in the hierarchical cluster analysis (HCA). The experimental and theoretical angles were analyzed by simple linear regression, and statistical parameters (correlation coefficients, significance, and predictability) were evaluated to determine the accuracy of the calculations. The statistical analysis exhibited a good correlation and high predictive power for the DFT (B3LYP) method in the 6-31G** basis set.展开更多
Through the long time track examination and disintegration to SF6 circuit breaker, we obtain the massive monitor data and massive pictures. The criteria of resuming insulation discharge failure conforming to CSO2/CH2S...Through the long time track examination and disintegration to SF6 circuit breaker, we obtain the massive monitor data and massive pictures. The criteria of resuming insulation discharge failure conforming to CSO2/CH2S>7, is quite broad to SO2 and the H2S concentration permission. Even if it reaches 100μL/L, it will not be in danger immediately to the safe operation of equipment. We may plan, arrange, and overhaul calmly. When obtaining the bare conductor overheating failure, it has not involved the resuming insulation. We may use the resuming insulation discharge failure criterion.展开更多
In this letter, the φ^6 ＋ φ^5 model in （D ＋ 1） dimensions can be solved by a truncated series method. A series of solitary solutions of the φ^6 ＋ φ^5 model in （D ＋ 1） dimensions have be obtained.
In the presented study, the laser butt-welding of Ti 6Al 4V is investigated using 2.2 kw CO2 laser. Ti 6Al 4V alloy has widespread application in various fields of industries including the medical, nuclear and aerospa...In the presented study, the laser butt-welding of Ti 6Al 4V is investigated using 2.2 kw CO2 laser. Ti 6Al 4V alloy has widespread application in various fields of industries including the medical, nuclear and aerospace. In this study, Response Surface Methodology (RSM) is employed to establish the design of experiments and to optimize the bead geometry. The relationships between the input laser-welding parameters (i.e. laser power, welding speed and focal point position) and the process responses (i.e. welded zone width, heat affected zone width, welded zone area, heat affected zone area and penetration depth) are investigated. The multi-response optimizations are used to optimize the welding process. The optimum welding conditions are identified in order to increase the productivity and minimize the total operating cost. The validation results demonstrate that the developed models are accurate with low percentages of error (less than 12.5%).展开更多
文摘The methods of determination of 6-methyl coumarin in cosmetics by HPLC-DAD and GC-FID were compared. The positive samples were confirmed by GC-MS. Methanol was used to extract 6-methyl coumarin from cosmetics. The chromatographic conditions of the two methods were optimized and the samples were determined. The recovery, precision, correlation coefficient, detection limit and quantitative limit of the two methods were compared. The results showed that the linear relationship between the two methods was good. The percentage recovery of standard addition by HPLC-DAD was 92.20%~102.80%, by RSD was 0.77%~2.00%;the detection limit was 0.05 mg/kg, the quantitative limit was 0.17 mg/kg. The percentage recovery of standard addition by GC-FID was 97.30%~103.28%, by RSD was 0.47%~4.87%;the detection limit was 1.3 mg/kg, the quantitative limit was 5.0 mg/kg. The detection limit by HPLC-DAD was lower and more sensitive than that by GC-FID , which also shows that HPLC-DAD has more advantages for the detection of low content samples.
文摘Objective To observe the clinical effect of acupuncture at Neiguan （内关 PC 6） in combination with respiratory reinforcing and reducing method for treatment of cardiac neurosis. Methods Thirty-eight patients with cardiac neurosis were selected for treatment through acupuncture at PC 6 in combination with respiratory reinforcing and reducing method. With twice per day, treated for 6 days per week, and 4 weeks as one course, the therapeutic effect was assessed after one course. Results Twenty-one cases were cured, 15 cases were remarkably improved, and 2 cases were failed. The total effective rate was 94.7%. Conclusion Acupuncture at PC 6 in combination with respiratory reinforcing and reducing method is effective in treatment of cardiac neurosis.
基金supported by the National Natural Science Foundation of China(No.11305225)the Science and Technology Service Network Initiative(KFJ-EW-STS-086)
文摘In this study,a high-throughput screening method was established through the 24-square deep-well microliter plate(MTP) fermentation and micro-plate detection for large-scale screening of the mutants.It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation.Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation.The filter paper activity and β-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23%separately compared with A.niger H11 by shaking flask fermentation,and it was genetically stable after being passaged to nine generations.The results indicate that the high-throughput screening method can be used for the quick breeding of A.niger with high cellulase activity.
基金supported by the National Natural Science Foundation of China(Grant 51975298)the Natural Science Foundation of Jiangsu Province(Grant BK20181301)the National Science Foundation of China(Grant 11874303).
文摘The structure parameters of 6-degree of freedom(DOF)vibration isolation platform have a significant effect on its performance.To make the designed vibration isolation platform perform well,non-dominanted sorting genetic algorithm version II(NSGA-II)was applied to optimize its structure based on the transfer matrix method for multibody systems.Firstly,the Jacobian matrix of 6-DOF vibration isolation platform was solved based on kinematics.Secondly,the transfer equation of 6-DOF vibration isolation system was established by the linear transfer matrix method for multibody systems.And the formula of its natural frequency was derived according to the boundary conditions of the system.Thirdly,the manipulability index was constructed based on a dimensionless Jacobian matrix.And a new performance index function was established considering the influence of dynamic isotropic and legs mass.Fourthly,genetic algorithm(GA)and NSGA-II were used to optimize the structure of the 6-DOF vibration isolation platform under the same conditions,respectively.It showed that NSGA-II had higher optimization efficiency,better calculation accuracy and shorter optimization time than that of GA.Finally,NSGA-II was adopted for multi-objective optimization design of 6-DOF vibration isolation platform based on the constraint conditions.Optimal Pareto solutions were obtained,which provides structural parameters for subsequent design work.Therefore,the proposed optimization method and the performance index in this paper provide a theoretical basis for the optimal design of relevant vibration isolation mechanism.
基金Funded in part by the Key Laboratory of Inorginic Coating MaterialsChinese Academy of Sciences(No.KLICM-2014-11)the Shanghai Municipal Natural Science Foundation Sponsored by Shanghai Municipal Science and Technology Commissions(No.15ZR1428300)
文摘Ti6Al4V substrates were anodized in a 0.5 mol/L H_2SO_4 solution at applied voltages of 90-140 V.A hydroxyapatite-titanium oxide（HA-TiO2）coating was then deposited on the anodized Ti6Al4 V substrates via a hydrothermal-electrochemicalmethod at a constant current.The obtained films and coatings were characterized by X-ray diffraction,scanning electron microscopy,energy-dispersive X-ray spectroscopy,and Fourier-transform infrared spectrometry.The microstructures of the porous films on the Ti6Al4 V substrates were studied to investigate the effect of the anodizing voltage on the phase and morphology of the HATiO_2 coating.The results indicated that both the phase composition and the morphology of the coatings were significantly influenced by changes in the anodizing voltage.HA-TiO_2 was directly precipitated onto the surface of the substrate when the applied voltage was between 110 and 140 V.The coatings had a gradient structure and the HA exhibited both needle-like and cotton-like structures.The amount of cotton-like HA structures decreased with an increase in voltage from 90 to 120 V,and then increased slightly when the voltage was higher than 120 V.The orientation index of the（002）plane of the coating was at a minimum when the Ti6Al4 V substrate was pretreated at 120 V.
基金financially supported by the Key Project of Sichuan provincial Education office(No.13ZA0183)Applied Basic Research Programs of Sichuan Provincial Science and Technology Office(No.2014JY0059)+1 种基金Foundation of Youth Science and Technology Innovation Team of Sichuan Province(No.2015TD0007)Program for New Century Excellent Talents in University of Ministof Education of China(No.NCET-13-0983)
文摘In this work, ZnSn（OH）6with a cubic structure is successfully synthesized by one-step hydrothermal method without any catalyst. The response and recovery characteristics of gas sensing were investigated against various gases via quartz crystal microbalance（QCM） at room temperature. The sensor exhibited high sensitivity and good selectivity toward CO gas. Moreover, a linear dependence of log^（àDelta F）about CO concentration was obtained. It is demonstrated that the QCM sensor coated cubic ZnSn（OH）6could be a suitable candidate for detecting CO.
文摘Pr0.6-x NdxCa0.4 FeO3-δ （ x = 0.0, 0.2, 0.3, 0.4, 0.5, 0.6） were synthesized using Pechini method. A number of studies were conducted concerning composition, specific area, crystalline structure and microstructure of the samples by means of FT-IR, BET, XRD TG-DTA and SEM. The results show that all the samples with different doping amounts of Pr^3＋ and Nd^3＋ on A-site are fine dispersed, and mean particle size less than 100 nm. The powders have good sinterability, and the relative density is 95% after sintered at 1200 ℃ for 2 h. It is found that all specimens are entirely single phase solid solutions with orthorhombic perovskite structure, the stable perovskitetype phase is formed completely after calcination at 900 ℃.
文摘In this paper, we evaluate semiempirical methods (AM1, PM3, and ZINDO), HF and DFT (B3LYP) in different basis sets to determine which method best describes the sign and magnitude of the geometrical parameters of artemisinin in the region of the endoperoxide ring compared to crystallographic data. We also classify these methods using statistical analysis. The results of PCA were based on three main components, explaining 98.0539% of the total variance, for the geometrical parameters C3O13, O1O2C3, O13C12C12a, and O2C3O13C12. The DFT method (B3LYP) corresponded well with the experimental data in the hierarchical cluster analysis (HCA). The experimental and theoretical angles were analyzed by simple linear regression, and statistical parameters (correlation coefficients, significance, and predictability) were evaluated to determine the accuracy of the calculations. The statistical analysis exhibited a good correlation and high predictive power for the DFT (B3LYP) method in the 6-31G** basis set.
文摘Through the long time track examination and disintegration to SF6 circuit breaker, we obtain the massive monitor data and massive pictures. The criteria of resuming insulation discharge failure conforming to CSO2/CH2S>7, is quite broad to SO2 and the H2S concentration permission. Even if it reaches 100μL/L, it will not be in danger immediately to the safe operation of equipment. We may plan, arrange, and overhaul calmly. When obtaining the bare conductor overheating failure, it has not involved the resuming insulation. We may use the resuming insulation discharge failure criterion.
基金The project supported by National Natural Science Foundation of China under Grant No. 10575026.Acknowledgments The author thanks Prof. S.Y. Lou for helpful discussions.
文摘In this letter, the φ^6 ＋ φ^5 model in （D ＋ 1） dimensions can be solved by a truncated series method. A series of solitary solutions of the φ^6 ＋ φ^5 model in （D ＋ 1） dimensions have be obtained.
文摘In the presented study, the laser butt-welding of Ti 6Al 4V is investigated using 2.2 kw CO2 laser. Ti 6Al 4V alloy has widespread application in various fields of industries including the medical, nuclear and aerospace. In this study, Response Surface Methodology (RSM) is employed to establish the design of experiments and to optimize the bead geometry. The relationships between the input laser-welding parameters (i.e. laser power, welding speed and focal point position) and the process responses (i.e. welded zone width, heat affected zone width, welded zone area, heat affected zone area and penetration depth) are investigated. The multi-response optimizations are used to optimize the welding process. The optimum welding conditions are identified in order to increase the productivity and minimize the total operating cost. The validation results demonstrate that the developed models are accurate with low percentages of error (less than 12.5%).
