A highly sensitive SPE-liquid/liquid extraction RPLC method has been developed for the analysis of 6β-hydroxycortisol and cortisol in the urine of cancer patients. Methods： After SPE column purification and liquid-l...A highly sensitive SPE-liquid/liquid extraction RPLC method has been developed for the analysis of 6β-hydroxycortisol and cortisol in the urine of cancer patients. Methods： After SPE column purification and liquid-liquid extraction, the sample test solutions were analyzed with RPLC using a C18 analytical column. This improved analytical method has been validated for linearity, accuracy （recovery from urine）, repeatability （within-day and between-day precision）, specificity, sensitivity, and stability. This SPE-liquid/liquid extraction-RPLC is rapid, simple, accurate and reproducible. The technique is particularly useful for monitoring the CYP3A activity of cancer patients in clinical settings. The results are expressed as the ratio of 6β-hydroxycortisol to cortisol. Results： The CYP3A activity from a total of 153 samples was measured using this improved method. Considerable variation in the CYP3A activity of different cancer patients has been documented. Thus, personalized medical treatment based on the individual metabolic enzyme activity level is necessary. Conclusion： This new analytical method facilitates such individualized medical treatments.展开更多
Purpose:To evaluate APT(APT)for its analgesic effects and influence on serum cortisol and IL-6 levels after cesarean section.Methods:108 puerperae prepared for cesarean section were randomly divided into three treatme...Purpose:To evaluate APT(APT)for its analgesic effects and influence on serum cortisol and IL-6 levels after cesarean section.Methods:108 puerperae prepared for cesarean section were randomly divided into three treatment groups:APT,patient-controlled intravenous analgesia(PCIA),and a combination of APT and PCIA.The degrees of incision pain(including pain at rest and pain evoked by changing position in bed)and oxytocin-mediated uterine cramping pain were determined using a visual analogue scale(VAS).The serum concentrations of cortisol and IL-6 were measured preoperatively and postoperatively.Results:Uterine cramping pain was lower in the APT group than the PCIA group and lowest in the combination therapy group.Incision pain was similar between the APT group and the PCIA group but lower in the combination therapy group.On the second morning after surgery,the serum concentrations of cortisol and IL-6 were similar between the APT group and the PCIA group but lower in the combination therapy group.The extent of pain was highly related to cortisol levels and moderately related to IL-6 levels.Conclusion:APT can relieve uterine cramping pain after cesarean section,and in combination with PCIA can decrease serum levels of cortisol and IL-6.展开更多
目的探讨全身麻醉下新生儿幽门环肌切开术复合不同剂量罗哌卡因骶管阻滞对动脉血气及血浆皮质醇、白细胞介素（IL）-6的影响。方法选择医院择期行幽门环肌切开术的足月儿59例,美国麻醉医师协会（ASA）分级ⅠⅡ级,按随机数字表法分为两...目的探讨全身麻醉下新生儿幽门环肌切开术复合不同剂量罗哌卡因骶管阻滞对动脉血气及血浆皮质醇、白细胞介素（IL）-6的影响。方法选择医院择期行幽门环肌切开术的足月儿59例,美国麻醉医师协会（ASA）分级ⅠⅡ级,按随机数字表法分为两组。对照组（28例）全身麻醉下复合0.10%罗哌卡因（1.0 m L/kg）骶管阻滞麻醉,研究组（31例）在全身麻醉下复合0.10%罗哌卡因（1.2 m L/kg）骶管阻滞麻醉。结果两组患者在气管拔管时间、苏醒时间及麻醉后监测治疗室（PACU）停留时间存在显著差异（P<0.01）;两组麻醉诱导前（T1）、麻醉10 min（T2）、术后（T3）、术后8 h（T4）时心率（HR）、血氧饱和度（SpO2）无差异（P>0.05）,T2及T3时收缩压（SBP）存在差异（P<0.05）,T3时舒张压（DBP）存在差异（P<0.05）;与T1时比较,对照组T2、T3、T4时血浆皮质醇和IL-6存在显著变化（P<0.01）,研究组T2、T3、T4时血浆皮质醇和IL-6无变化（P>0.05）,组间比较显著差异（P<0.01）;组间低血压发生率存在差异（P<0.05）。结论全身麻醉下新生儿幽门环肌切开术复合1.2 m L/kg罗哌卡因骶管阻滞对血浆皮质醇、IL-6影响不大,并发症少,值得推广。展开更多
基金supported by the Shanghai Pharmaceutical Association
文摘A highly sensitive SPE-liquid/liquid extraction RPLC method has been developed for the analysis of 6β-hydroxycortisol and cortisol in the urine of cancer patients. Methods： After SPE column purification and liquid-liquid extraction, the sample test solutions were analyzed with RPLC using a C18 analytical column. This improved analytical method has been validated for linearity, accuracy （recovery from urine）, repeatability （within-day and between-day precision）, specificity, sensitivity, and stability. This SPE-liquid/liquid extraction-RPLC is rapid, simple, accurate and reproducible. The technique is particularly useful for monitoring the CYP3A activity of cancer patients in clinical settings. The results are expressed as the ratio of 6β-hydroxycortisol to cortisol. Results： The CYP3A activity from a total of 153 samples was measured using this improved method. Considerable variation in the CYP3A activity of different cancer patients has been documented. Thus, personalized medical treatment based on the individual metabolic enzyme activity level is necessary. Conclusion： This new analytical method facilitates such individualized medical treatments.
基金The study is the(Key)Item of Health Science and Technology Program in Hangzhou(Code:2013Z06).
文摘Purpose:To evaluate APT(APT)for its analgesic effects and influence on serum cortisol and IL-6 levels after cesarean section.Methods:108 puerperae prepared for cesarean section were randomly divided into three treatment groups:APT,patient-controlled intravenous analgesia(PCIA),and a combination of APT and PCIA.The degrees of incision pain(including pain at rest and pain evoked by changing position in bed)and oxytocin-mediated uterine cramping pain were determined using a visual analogue scale(VAS).The serum concentrations of cortisol and IL-6 were measured preoperatively and postoperatively.Results:Uterine cramping pain was lower in the APT group than the PCIA group and lowest in the combination therapy group.Incision pain was similar between the APT group and the PCIA group but lower in the combination therapy group.On the second morning after surgery,the serum concentrations of cortisol and IL-6 were similar between the APT group and the PCIA group but lower in the combination therapy group.The extent of pain was highly related to cortisol levels and moderately related to IL-6 levels.Conclusion:APT can relieve uterine cramping pain after cesarean section,and in combination with PCIA can decrease serum levels of cortisol and IL-6.
文摘目的探讨全身麻醉下新生儿幽门环肌切开术复合不同剂量罗哌卡因骶管阻滞对动脉血气及血浆皮质醇、白细胞介素（IL）-6的影响。方法选择医院择期行幽门环肌切开术的足月儿59例,美国麻醉医师协会（ASA）分级ⅠⅡ级,按随机数字表法分为两组。对照组（28例）全身麻醉下复合0.10%罗哌卡因（1.0 m L/kg）骶管阻滞麻醉,研究组（31例）在全身麻醉下复合0.10%罗哌卡因（1.2 m L/kg）骶管阻滞麻醉。结果两组患者在气管拔管时间、苏醒时间及麻醉后监测治疗室（PACU）停留时间存在显著差异（P<0.01）;两组麻醉诱导前（T1）、麻醉10 min（T2）、术后（T3）、术后8 h（T4）时心率（HR）、血氧饱和度（SpO2）无差异（P>0.05）,T2及T3时收缩压（SBP）存在差异（P<0.05）,T3时舒张压（DBP）存在差异（P<0.05）;与T1时比较,对照组T2、T3、T4时血浆皮质醇和IL-6存在显著变化（P<0.01）,研究组T2、T3、T4时血浆皮质醇和IL-6无变化（P>0.05）,组间比较显著差异（P<0.01）;组间低血压发生率存在差异（P<0.05）。结论全身麻醉下新生儿幽门环肌切开术复合1.2 m L/kg罗哌卡因骶管阻滞对血浆皮质醇、IL-6影响不大,并发症少,值得推广。
