Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integ...Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integrate public data analysis with experimental validation to identify novel prognostic biomarkers and therapeutic targets for lung adenocarcinoma(LUAD).Methods:We analyzed RNA and protein databases to assess the expression levels of cytochrome C oxidase 5B(COX5B)in LUAD.Several computational algorithms were employed to investigate the relationship between COX5B and immune infiltration in LUAD.To further elucidate the role of COX5B in LUAD,we utilized multiple experimental approaches,including quantitative reverse transcription PCR assays,western blot,immunohistochemistry,electron microscopy,flow cytometry,and EdU proliferation assays.Results:We revealed that COX5B was significantly elevated in LUAD and positively correlated with poor prognosis of LUAD patients.Analysis of co-expression network indicated that COX5B may take part in the intracellular adenosine triphosphate(ATP)synthesis through the oxidative phosphorylation pathway.There was a negative correlation between COX5B expression and immune infiltration in LUAD.Furthermore,we validated that COX5B levels were significantly elevated in both LUAD tissues and cell lines.Specifically,immunohistochemistry(IHC)assays revealed a 2.32-fold increase of COX5B in tumor tissues compared to that in adjacent normal tissues(p=0.0044).Additionally,COX5B knockdown disrupted the redox homeostasis,ultimately suppressed the proliferation of LUAD cells.Subsequent investigations demonstrated that berberine effectively targeted COX5B,diminishing its protein expression and consequently inhibiting cell proliferation and tumor growth in LUAD.Conclusions:This study established that upregulated COX5B was positive associated with poor patient prognosis in LUAD,elucidating the mechanisms by which berberine targets COX5B to inhibit tumor growth,thereby providing a novel therapeutic target and strategy for the clinical management of LUAD.展开更多
针对目前矿石检测技术准确性不高、效率低、实时性差以及环境适应性弱等问题,提出了一种基于香橙派的矿石识别系统。该系统利用高清摄像头实时捕获矿石图像,并通过香橙派Orange Pi 5B开发板搭载YOLOv4-tiny-tf2模型处理图像数据,实现矿...针对目前矿石检测技术准确性不高、效率低、实时性差以及环境适应性弱等问题,提出了一种基于香橙派的矿石识别系统。该系统利用高清摄像头实时捕获矿石图像,并通过香橙派Orange Pi 5B开发板搭载YOLOv4-tiny-tf2模型处理图像数据,实现矿石种类的高效准确识别。结果表明:轻量化的YOLOv4-tiny-tf2模型在不牺牲模型精度的情况下简化了其结构,更适合在配置较低的设备上部署,具备了较高的便携性;相较于传统的人工视觉检测和化学分析方法,基于香橙派Orange Pi 5B的矿石识别系统能够实时识别矿石,识别准确率达到93.75%,具有较高的可靠性和稳定性。展开更多
基金supported by grants from the Guangxi Natural Science Foundation(2024GXNSFAA010150)the Guangdong Basic and Applied Basic Research Foundation(2022A1515111167).
文摘Background:A significant proportion of patients still cannot benefit from existing targeted therapies and immunotherapies,making the search for new treatment strategies extremely urgent.In this study,we combined integrate public data analysis with experimental validation to identify novel prognostic biomarkers and therapeutic targets for lung adenocarcinoma(LUAD).Methods:We analyzed RNA and protein databases to assess the expression levels of cytochrome C oxidase 5B(COX5B)in LUAD.Several computational algorithms were employed to investigate the relationship between COX5B and immune infiltration in LUAD.To further elucidate the role of COX5B in LUAD,we utilized multiple experimental approaches,including quantitative reverse transcription PCR assays,western blot,immunohistochemistry,electron microscopy,flow cytometry,and EdU proliferation assays.Results:We revealed that COX5B was significantly elevated in LUAD and positively correlated with poor prognosis of LUAD patients.Analysis of co-expression network indicated that COX5B may take part in the intracellular adenosine triphosphate(ATP)synthesis through the oxidative phosphorylation pathway.There was a negative correlation between COX5B expression and immune infiltration in LUAD.Furthermore,we validated that COX5B levels were significantly elevated in both LUAD tissues and cell lines.Specifically,immunohistochemistry(IHC)assays revealed a 2.32-fold increase of COX5B in tumor tissues compared to that in adjacent normal tissues(p=0.0044).Additionally,COX5B knockdown disrupted the redox homeostasis,ultimately suppressed the proliferation of LUAD cells.Subsequent investigations demonstrated that berberine effectively targeted COX5B,diminishing its protein expression and consequently inhibiting cell proliferation and tumor growth in LUAD.Conclusions:This study established that upregulated COX5B was positive associated with poor patient prognosis in LUAD,elucidating the mechanisms by which berberine targets COX5B to inhibit tumor growth,thereby providing a novel therapeutic target and strategy for the clinical management of LUAD.
文摘针对目前矿石检测技术准确性不高、效率低、实时性差以及环境适应性弱等问题,提出了一种基于香橙派的矿石识别系统。该系统利用高清摄像头实时捕获矿石图像,并通过香橙派Orange Pi 5B开发板搭载YOLOv4-tiny-tf2模型处理图像数据,实现矿石种类的高效准确识别。结果表明:轻量化的YOLOv4-tiny-tf2模型在不牺牲模型精度的情况下简化了其结构,更适合在配置较低的设备上部署,具备了较高的便携性;相较于传统的人工视觉检测和化学分析方法,基于香橙派Orange Pi 5B的矿石识别系统能够实时识别矿石,识别准确率达到93.75%,具有较高的可靠性和稳定性。
