Detecting remote homology proteins is a challenging problem for both basic research and drug development. Although there are a couple of methods to deal with this problem, the benchmark datasets based on which the exi...Detecting remote homology proteins is a challenging problem for both basic research and drug development. Although there are a couple of methods to deal with this problem, the benchmark datasets based on which the existing methods were trained and tested contain many high homologous samples as reflected by the fact that the cutoff threshold was set at 95%. In this study, we reconstructed the benchmark dataset by setting the threshold at 40%, meaning none of the proteins included in the benchmark dataset has more than 40% pairwise sequence identity with any other in the same subset. Using the new benchmark dataset, we proposed a new predictor called “dRHP-GreyFun” based on the grey modeling and functional domain approach. Rigorous cross-validations have indicated that the new predictor is superior to its counterparts in both enhancing success rates and reducing computational cost. The predictor can be downloaded from https://github.com/jcilwz/dRHP-GreyFun.展开更多
Since the 5-steps rule was proposed in 2011, it has been widely used in many areas of molecular biology, both theoretical and experimental. It can be even used to deal with the commercial problems and bank systems, as...Since the 5-steps rule was proposed in 2011, it has been widely used in many areas of molecular biology, both theoretical and experimental. It can be even used to deal with the commercial problems and bank systems, as well as material science systems. Just like the machine-learning algorithms, it is the jade for nearly all the statistical systems.展开更多
In this current minireview, the cradle of the “5-steps rule” or “5-step rules”, along with its essence and advances, has been recalled. Born in 2011, its impacts on molecular biology are both substantial and rapid...In this current minireview, the cradle of the “5-steps rule” or “5-step rules”, along with its essence and advances, has been recalled. Born in 2011, its impacts on molecular biology are both substantial and rapid, fully indicating the “5-steps rule” is no double a remarkable and profound milestone in molecular biology.展开更多
In this short review paper, the significant and profound impacts of the 5-steps rule have <span style="mso-bookmark:OLE_LINK2;"><span style="mso-bookmark:OLE_LINK1;"><span lang="...In this short review paper, the significant and profound impacts of the 5-steps rule have <span style="mso-bookmark:OLE_LINK2;"><span style="mso-bookmark:OLE_LINK1;"><span lang="EN-US" style="line-height: 97%;font-family:;" capt",serif;font-size:11pt;"="" pro="" minion="">been briefly recalled with crystal clear convincingness.</span></span></span>展开更多
A novel technology of two-step fast microwave-assisted pyrolysis（f MAP） of corn stover for bio-oil production was investigated in the presence of microwave absorbent（Si C） and HZSM-5catalyst. Effects of f MAP temp...A novel technology of two-step fast microwave-assisted pyrolysis（f MAP） of corn stover for bio-oil production was investigated in the presence of microwave absorbent（Si C） and HZSM-5catalyst. Effects of f MAP temperature and catalyst-to-biomass ratio on bio-oil yield and chemical components were examined. The results showed that this technology, employing microwave, microwave absorbent and HZSM-5 catalyst, was effective and promising for biomass fast pyrolysis. The f MAP temperature of 500°C was considered the optimum condition for maximum yield and best quality of bio-oil. Besides, the bio-oil yield decreased linearly and the chemical components in bio-oil were improved sequentially with the increase of catalyst-to-biomass ratio from 1：100 to 1：20. The elemental compositions of bio-char were also determined. Additionally, compared to one-step f MAP process, two-step f MAP could promote the bio-oil quality with a smaller catalyst-to-biomass ratio.展开更多
文摘Detecting remote homology proteins is a challenging problem for both basic research and drug development. Although there are a couple of methods to deal with this problem, the benchmark datasets based on which the existing methods were trained and tested contain many high homologous samples as reflected by the fact that the cutoff threshold was set at 95%. In this study, we reconstructed the benchmark dataset by setting the threshold at 40%, meaning none of the proteins included in the benchmark dataset has more than 40% pairwise sequence identity with any other in the same subset. Using the new benchmark dataset, we proposed a new predictor called “dRHP-GreyFun” based on the grey modeling and functional domain approach. Rigorous cross-validations have indicated that the new predictor is superior to its counterparts in both enhancing success rates and reducing computational cost. The predictor can be downloaded from https://github.com/jcilwz/dRHP-GreyFun.
文摘Since the 5-steps rule was proposed in 2011, it has been widely used in many areas of molecular biology, both theoretical and experimental. It can be even used to deal with the commercial problems and bank systems, as well as material science systems. Just like the machine-learning algorithms, it is the jade for nearly all the statistical systems.
文摘In this current minireview, the cradle of the “5-steps rule” or “5-step rules”, along with its essence and advances, has been recalled. Born in 2011, its impacts on molecular biology are both substantial and rapid, fully indicating the “5-steps rule” is no double a remarkable and profound milestone in molecular biology.
文摘In this short review paper, the significant and profound impacts of the 5-steps rule have <span style="mso-bookmark:OLE_LINK2;"><span style="mso-bookmark:OLE_LINK1;"><span lang="EN-US" style="line-height: 97%;font-family:;" capt",serif;font-size:11pt;"="" pro="" minion="">been briefly recalled with crystal clear convincingness.</span></span></span>
基金supported by the National Basic Research Program (973) of China (No. 2013CB228106)the National Natural Science Fund Program of China (No. 51276040)+4 种基金the Scientific Research Foundation of Graduate School of Southeast University (No. YBJJ1430)the Fundamental Research Funds for the Central Universities, the Scientific Innovation Research Program of College Graduate in Jiangsu Province (No. KYLX_0183)China Scholarship Council, as well as Minnesota Environment and Natural Resources Trust FundNorth Central Regional Sun Grant Center at South Dakota State University through a grant provided by the US Department of Agriculture (No. 2013-38502-21424)a grant provided by the US Department of Transportation, Office of the Secretary (No. DTOS59-07-G-00054)
文摘A novel technology of two-step fast microwave-assisted pyrolysis（f MAP） of corn stover for bio-oil production was investigated in the presence of microwave absorbent（Si C） and HZSM-5catalyst. Effects of f MAP temperature and catalyst-to-biomass ratio on bio-oil yield and chemical components were examined. The results showed that this technology, employing microwave, microwave absorbent and HZSM-5 catalyst, was effective and promising for biomass fast pyrolysis. The f MAP temperature of 500°C was considered the optimum condition for maximum yield and best quality of bio-oil. Besides, the bio-oil yield decreased linearly and the chemical components in bio-oil were improved sequentially with the increase of catalyst-to-biomass ratio from 1：100 to 1：20. The elemental compositions of bio-char were also determined. Additionally, compared to one-step f MAP process, two-step f MAP could promote the bio-oil quality with a smaller catalyst-to-biomass ratio.
