Interleukin-5 (IL-5),expressed primarily by type-2 T heler (Th2) cell,plays an essential role in the devel-opment of allergic diseases, such as allergic asthma. Histone acetyltransferase CBP/p300 remodels chromatin by...Interleukin-5 (IL-5),expressed primarily by type-2 T heler (Th2) cell,plays an essential role in the devel-opment of allergic diseases, such as allergic asthma. Histone acetyltransferase CBP/p300 remodels chromatin by acety-lating histones, resulting in open structure of chromatin and active transcription. Adenovirus protein E1A inhibits the activity of CBP/p300. In this study,we analysed the effects of E1A on IL-5 gene promoter/luciferase reporter activity. The results showed that E1A protein inhibited the activity of PMA/ionomycin-stimulated IL-5 gene promoter/luiferase reporter construct. In contrast, overexpression of the CBP/p300-binding defective E1A △2-36 protein did not inhibit IL-5 gene promoter activity. These data demonstrated for the first time that transcriptional coactivator CBP/p300 was involved in the activation of IL-5 gene promoter. E1A protein can modulate CBP/p300 function to activate the transcription of IL-5 gene promoter/luciferase reporter plasmid. Furthermore, in collaboration with transcription factor C/EBP, CBP/p300 activated IL-5 gene pro-moter/luciferase reporter expression. This study provides further insight into the mechanisms of transcriptional regu-lation of IL-5 gene.展开更多
文摘Interleukin-5 (IL-5),expressed primarily by type-2 T heler (Th2) cell,plays an essential role in the devel-opment of allergic diseases, such as allergic asthma. Histone acetyltransferase CBP/p300 remodels chromatin by acety-lating histones, resulting in open structure of chromatin and active transcription. Adenovirus protein E1A inhibits the activity of CBP/p300. In this study,we analysed the effects of E1A on IL-5 gene promoter/luciferase reporter activity. The results showed that E1A protein inhibited the activity of PMA/ionomycin-stimulated IL-5 gene promoter/luiferase reporter construct. In contrast, overexpression of the CBP/p300-binding defective E1A △2-36 protein did not inhibit IL-5 gene promoter activity. These data demonstrated for the first time that transcriptional coactivator CBP/p300 was involved in the activation of IL-5 gene promoter. E1A protein can modulate CBP/p300 function to activate the transcription of IL-5 gene promoter/luciferase reporter plasmid. Furthermore, in collaboration with transcription factor C/EBP, CBP/p300 activated IL-5 gene pro-moter/luciferase reporter expression. This study provides further insight into the mechanisms of transcriptional regu-lation of IL-5 gene.
