High spatiotemporal resolution infrared radiances from FY-4A/AGRI(Advanced Geostationary Radiation Imager)can provide crucial information for rapidly developing severe convective weather.This study established a symme...High spatiotemporal resolution infrared radiances from FY-4A/AGRI(Advanced Geostationary Radiation Imager)can provide crucial information for rapidly developing severe convective weather.This study established a symmetric observation error model that differentiates between land and sea for FY-4A/AGRI all-sky assimilation,developed an all-sky assimilation scheme for FY-4A/AGRI based on hydrometeor control variables,and investigated the impacts of all-sky FY-4A/AGRI water vapor channels at different altitudes and rapid-update assimilation at different frequencies on the assimilation and forecasting of a severe convective weather event.Results show that simultaneous assimilation of two water vapor channels can enhance precipitation forecasts compared to single-channel assimilation,which is mainly attributable to a more accurate analysis of water vapor and hydrometeor information.Experiments with different assimilation frequencies demonstrate that the hourly assimilation frequency,compared to other frequencies,incorporates the high-frequency information from AGRI while reducing the impact of spurious oscillations caused by excessively high-frequency assimilation.This hourly assimilation frequency reduces the incoordination among thermal,dynamical,and water vapor conditions caused by excessively fast or slow assimilation frequencies,thus improving the forecast accuracy compared to other frequencies.展开更多
Contrary to the adult central nervous system,the peripheral nervous system has an intrinsic ability to regenerate that relies on the expression of regenerationassociated genes,such as some kinesin family members.Kines...Contrary to the adult central nervous system,the peripheral nervous system has an intrinsic ability to regenerate that relies on the expression of regenerationassociated genes,such as some kinesin family members.Kinesins contribute to nerve regeneration through the transport of specific cargo,such as proteins and membrane components,from the cell body towards the axon periphery.We show here that KIF4A,associated with neurodevelopmental disorders and previously believed to be only expressed during development,is also expressed in the adult vertebrate nervous system and up-regulated in injured peripheral nervous system cells.KIF4A is detected both in the cell bodies and regrowing axons of injured neurons,consistent with its function as an axonal transporter of cargoes such asβ1-integrin and L1CAM.Our study further demonstrates that KIF4A levels are greatly increased in Schwann cells from injured distal nerve stumps,particularly at a time when they are reprogrammed into an essential proliferative repair phenotype.Moreover,Kif4a m RNA levels were approximately~6-fold higher in proliferative cultured Schwann cells compared with non-proliferative ones.A hypothesized function for Kif4a in Schwann cell proliferation was further confirmed by Kif4a knockdown,as this significantly reduced Schwann cell proliferation in vitro.Our findings show that KIF4A is expressed in adult vertebrate nervous systems and is up-regulated following peripheral injury.The timing of KIF4A up-regulation,its location during regeneration,and its proliferative role,all suggest a dual role for this protein in neuroregeneration that is worth exploring in the future.展开更多
目的:分析子宫内膜癌组织中驱动蛋白家族4A(kinesin family 4A,KIF4A)、三结构域蛋白44(tripartite motif-containing protein 44,TRIM44)表达水平及其与临床病理特征的关系。方法:选择2022年3月—2025年2月徐州市妇幼保健院收治的79例...目的:分析子宫内膜癌组织中驱动蛋白家族4A(kinesin family 4A,KIF4A)、三结构域蛋白44(tripartite motif-containing protein 44,TRIM44)表达水平及其与临床病理特征的关系。方法:选择2022年3月—2025年2月徐州市妇幼保健院收治的79例子宫内膜癌患者。本研究对癌组织与癌旁组织中KIF4A、TRIM44的表达差异进行了比较分析,并进一步探讨其表达水平与患者临床病理特征之间的关系。结果:癌组织中KIF4A、TRIM44阳性表达率均高于癌旁组织(P<0.05);在国际妇产科联盟(FIGO)分期为Ⅲ期、存在淋巴结转移及肌层浸润深度≥1/2的患者中,其癌组织中KIF4A与TRIM44的阳性表达比例明显高于FIGOⅠ~Ⅱ期、无淋巴结转移且肌层浸润深度<1/2的患者(P<0.05)。结论:在子宫内膜癌组织中,KIF4A与TRIM44水平均呈高表达,其表达情况与淋巴结转移、肌层浸润程度及FIGO分期紧密相关。展开更多
基金supported by the National Key R&D Program of China(Grant No.2022YFC3080500)the National Natural Science Foundation of China(Grant Nos.U2142208,42475158,and 42105149)the High-Performance Computing Center of Nanjing University of Information Science&Technology for supporting this work。
文摘High spatiotemporal resolution infrared radiances from FY-4A/AGRI(Advanced Geostationary Radiation Imager)can provide crucial information for rapidly developing severe convective weather.This study established a symmetric observation error model that differentiates between land and sea for FY-4A/AGRI all-sky assimilation,developed an all-sky assimilation scheme for FY-4A/AGRI based on hydrometeor control variables,and investigated the impacts of all-sky FY-4A/AGRI water vapor channels at different altitudes and rapid-update assimilation at different frequencies on the assimilation and forecasting of a severe convective weather event.Results show that simultaneous assimilation of two water vapor channels can enhance precipitation forecasts compared to single-channel assimilation,which is mainly attributable to a more accurate analysis of water vapor and hydrometeor information.Experiments with different assimilation frequencies demonstrate that the hourly assimilation frequency,compared to other frequencies,incorporates the high-frequency information from AGRI while reducing the impact of spurious oscillations caused by excessively high-frequency assimilation.This hourly assimilation frequency reduces the incoordination among thermal,dynamical,and water vapor conditions caused by excessively fast or slow assimilation frequencies,thus improving the forecast accuracy compared to other frequencies.
基金supported by the Portuguese Foundation for Science and Technology(FCT),Centro 2020 and Portugol2020 and the EU FEDER program,via the project GoBack to SIV(PTDC/CVT-CVT/32261/2017,CENTRO-01-0145-FEDER-032261)the doctoral grants of PDC(SFRH/BD/139974/2018)and BMS(2020.06525.BD and DOI 10.54499/2020.06525.BD)+5 种基金the post-doctoral grant to JPF(SFRH/BPD/113359/2015-program-contract described in paragraphs 4,5,6 of art.23 of Law no.100157/2016,of August 29,as amended by Law no.57/2017 of July 2019),the project PTDC/MED-NEU/1677/2021 to JBRthe Institute of Biomedicine iBiMED(UIDB/04501/2020 and DOI 10.54499/UIDB/04501/2020,UIDP/04501/2020 and DOI 10.54499/UIDP/04501/2020)its LiM Bioimaging Facility-a PPBI node(POCI-01-0145-FEDER-022122)supported by the Research Commission of the Medical Faculty of the Heinrich-Heine-University(HHU)Düsseldorf,of the Biologisch-Medizinisches Forschungszentrum(BMFZ)of HHUfinanced by the Spanish"Plan Nacional de Investigacion Cientifica,Desarrollo e Innovacion Tecnologica,Ministerio de Economia y Competitividad(Instituto de Salud CarlosⅢ)",co-financed by the European Union(FEDER program),(grant FIS P/20/00318 and FIS P23/00337 to VC)grant CPP2021-009070 to VC by the"Proyectos de colaboracion publico-privada,Plan de Investigacion Cientifica,Tecnica y de inovacion 2021-2023,Ministerio de Ciencia e Innovacion,Union Europea,Agencia Estatal de Investigacion,Espana"。
文摘Contrary to the adult central nervous system,the peripheral nervous system has an intrinsic ability to regenerate that relies on the expression of regenerationassociated genes,such as some kinesin family members.Kinesins contribute to nerve regeneration through the transport of specific cargo,such as proteins and membrane components,from the cell body towards the axon periphery.We show here that KIF4A,associated with neurodevelopmental disorders and previously believed to be only expressed during development,is also expressed in the adult vertebrate nervous system and up-regulated in injured peripheral nervous system cells.KIF4A is detected both in the cell bodies and regrowing axons of injured neurons,consistent with its function as an axonal transporter of cargoes such asβ1-integrin and L1CAM.Our study further demonstrates that KIF4A levels are greatly increased in Schwann cells from injured distal nerve stumps,particularly at a time when they are reprogrammed into an essential proliferative repair phenotype.Moreover,Kif4a m RNA levels were approximately~6-fold higher in proliferative cultured Schwann cells compared with non-proliferative ones.A hypothesized function for Kif4a in Schwann cell proliferation was further confirmed by Kif4a knockdown,as this significantly reduced Schwann cell proliferation in vitro.Our findings show that KIF4A is expressed in adult vertebrate nervous systems and is up-regulated following peripheral injury.The timing of KIF4A up-regulation,its location during regeneration,and its proliferative role,all suggest a dual role for this protein in neuroregeneration that is worth exploring in the future.
文摘目的:分析子宫内膜癌组织中驱动蛋白家族4A(kinesin family 4A,KIF4A)、三结构域蛋白44(tripartite motif-containing protein 44,TRIM44)表达水平及其与临床病理特征的关系。方法:选择2022年3月—2025年2月徐州市妇幼保健院收治的79例子宫内膜癌患者。本研究对癌组织与癌旁组织中KIF4A、TRIM44的表达差异进行了比较分析,并进一步探讨其表达水平与患者临床病理特征之间的关系。结果:癌组织中KIF4A、TRIM44阳性表达率均高于癌旁组织(P<0.05);在国际妇产科联盟(FIGO)分期为Ⅲ期、存在淋巴结转移及肌层浸润深度≥1/2的患者中,其癌组织中KIF4A与TRIM44的阳性表达比例明显高于FIGOⅠ~Ⅱ期、无淋巴结转移且肌层浸润深度<1/2的患者(P<0.05)。结论:在子宫内膜癌组织中,KIF4A与TRIM44水平均呈高表达,其表达情况与淋巴结转移、肌层浸润程度及FIGO分期紧密相关。
