Since the 1990s,continuous technical and scientific advances have defied the diffraction limit in microscopy and enabled three-dimensional(3D)super-resolution imaging.An important milestone in this pursuit is the cohe...Since the 1990s,continuous technical and scientific advances have defied the diffraction limit in microscopy and enabled three-dimensional(3D)super-resolution imaging.An important milestone in this pursuit is the coherent utilization of two opposing objectives(4Pi geometry)and its combination with superresolution microscopy.Herein,we review the recent progress in 4Pi nanoscopy,which provides a 3D,non-invasive,diffraction-unlimited,and isotropic resolution in transparent samples.This review includes both the targeted and stochastic switching modalities of 4Pi nanoscopy.The schematics,principles,applications,and future potential of 4Pi nanoscopy are discussed in detail.展开更多
Recently,we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules asfluorescent labels can enable optical resolutions far beyond the diffrac...Recently,we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules asfluorescent labels can enable optical resolutions far beyond the diffraction-limit.Here,we show that by combining the 4Pi microscopy and the novelfluorescent label,it is possible to completely suppress the sidelobes in 4Pi focal spot and significantly enhance the optical resolution in the axial direction.展开更多
Spatiotemporal optical vortices(STOVs)are distinguished by their intricate phase structures and orbital angular momentum properties.This study demonstrates a coherent 4Pi-focused method generating STOV arrays by tight...Spatiotemporal optical vortices(STOVs)are distinguished by their intricate phase structures and orbital angular momentum properties.This study demonstrates a coherent 4Pi-focused method generating STOV arrays by tightly focusing the wave packets carrying spatiotemporal vortices and an additional spatial vortex.Numerical simulations reveal that the structural characteristics of the STOV array are governed by the interplay between the spatiotemporal and spatial topological charges of the incident wave packet.Notably,the introduction of spin angular momentum into the wave packet induces spin-orbit coupling,resulting in a distinctive tilt of the STOV array.These findings open potential pathways for high-dimensional optical manipulation and advanced optical information multiplexing.展开更多
为了解决森林火灾、盗伐树木和伤害野生动物等林业管理方面的问题,设计了基于ROS的智能林业巡逻机器人。该机器人采用Raspberry Pi 4B作为主控、STM32F103RCT6拓展板作为辅控,主要硬件采用深度相机、激光雷达等,多种传感器协同对各种复...为了解决森林火灾、盗伐树木和伤害野生动物等林业管理方面的问题,设计了基于ROS的智能林业巡逻机器人。该机器人采用Raspberry Pi 4B作为主控、STM32F103RCT6拓展板作为辅控,主要硬件采用深度相机、激光雷达等,多种传感器协同对各种复杂环境进行环境感知。系统使用Ubuntu18.04,在电脑终端利用VNC远程控制平台对机器人进行操控。机器人具备自主巡逻、实时监控和物体识别等功能,并选用Cartographer建图算法进行自主导航,利用深度学习算法YOLOv4-tiny进行物体识别。实验测试结果表明,智能林业巡逻机器人运行稳定,可有效解决林业管理的问题,具有很高的实际应用价值。展开更多
Epidemiological studies have indicated that branched-chain amino acids(BCAAs)increased and gut microbiota disordered in type 2 diabetes mellitus(T2DM).This study aimed to investigate the mechanism of Lactiplantibacill...Epidemiological studies have indicated that branched-chain amino acids(BCAAs)increased and gut microbiota disordered in type 2 diabetes mellitus(T2DM).This study aimed to investigate the mechanism of Lactiplantibacillus plantarum strain 84-3(Lp84-3)combined with Staphylococcus aureus bacteriophage on ameliorating T2DM.Here we perform a case-control study and identify that Staphylococcus_phage was inversely correlated with fasting blood glucose(FBG).It revealed that Lp84-3 could inhibit the growth of S.aureus,and Lp84-3 contains BCAAs degradation enzymes in its genome.Furthermore,Lp84-3 alone or combined with S.aureus bacteriophage interventions can improve blood glucose,insulin resistance,triglycerides,interleukin-1β,tumor necrosis factor-α(TNF-α),BCAAs,and acetyllactate synthase(ALS)in db/db mice.Lp84-3 and S.aureus bacteriophage decreased S.aureus,Malacoplasma iowae,and Oscillibacter sp.,and increased some beneficial such as L.plantarum and Muribaculaceae bacterium.Transcriptomic analyses revealed that Lp84-3 and S.aureus bacteriophage activated the PI3K/AKT/GLUT4 signaling pathway and upregulated key genes of Il22,Hgf,Col6a1,Gh,Itga10,Fgf23,and Prl involved in glucose metabolism in hypothalamus.Collectively,Lp84-3 and S.aureus bacteriophage alleviate T2DM by modulating gut microbiota and enhancing glucose metabolism in hypothalamus,supporting its potential use as a promising functional compound microecological agent for alleviating T2DM.展开更多
针对大型气象监测站成本高、覆盖范围有限、灵活性差的问题,设计了一套面向局部定点区域的智能化微型气象监测系统。首先,基于树莓派Raspberry Pi 4B平台开发了一套微型气象数据采集装置,可实现多种气象参数的实时采集与传输。然后,基...针对大型气象监测站成本高、覆盖范围有限、灵活性差的问题,设计了一套面向局部定点区域的智能化微型气象监测系统。首先,基于树莓派Raspberry Pi 4B平台开发了一套微型气象数据采集装置,可实现多种气象参数的实时采集与传输。然后,基于Qt开发了一款服务端软件,实现了针对前端采集气象数据的接收、存储、管理和可视化显示。前端采集系统实现了多种气象信息的综合检测,且系统功能可靠稳定;服务端系统实现的大数据管理和图形化显示,增强了系统可读性和实用性。该系统不仅能为气象数据的联合分析提供可靠数据源,也为业界开发相关商业产品提供了借鉴思路。展开更多
基金financially supported by the grants from National Key Research and Development Program of China (2018YFA0701400 and 2018YFE0119000)the Fundamental Research Funds for the Central Universities (2019QNA5006)+2 种基金ZJU-Sunny Photonics Innovation Center (2019-01)Zhejiang Provincial Natural Science Foundation of China (LR18H180001)startup grant from Southern University of Science and Technology
文摘Since the 1990s,continuous technical and scientific advances have defied the diffraction limit in microscopy and enabled three-dimensional(3D)super-resolution imaging.An important milestone in this pursuit is the coherent utilization of two opposing objectives(4Pi geometry)and its combination with superresolution microscopy.Herein,we review the recent progress in 4Pi nanoscopy,which provides a 3D,non-invasive,diffraction-unlimited,and isotropic resolution in transparent samples.This review includes both the targeted and stochastic switching modalities of 4Pi nanoscopy.The schematics,principles,applications,and future potential of 4Pi nanoscopy are discussed in detail.
基金J.Chen acknowledges thefinancial support from NSFC(Grant No.61078016).
文摘Recently,we theoretically demonstrate that utilization of silica nanobeads co-doped with Cy3 and Cy5 molecules instead of single dye molecules asfluorescent labels can enable optical resolutions far beyond the diffraction-limit.Here,we show that by combining the 4Pi microscopy and the novelfluorescent label,it is possible to completely suppress the sidelobes in 4Pi focal spot and significantly enhance the optical resolution in the axial direction.
基金supported by the National Natural Science Foundation of China(Nos.12274074 and 12134013)the Natural Science Foundation of Jiangsu Province(No.BK20242024)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX25_0437)。
文摘Spatiotemporal optical vortices(STOVs)are distinguished by their intricate phase structures and orbital angular momentum properties.This study demonstrates a coherent 4Pi-focused method generating STOV arrays by tightly focusing the wave packets carrying spatiotemporal vortices and an additional spatial vortex.Numerical simulations reveal that the structural characteristics of the STOV array are governed by the interplay between the spatiotemporal and spatial topological charges of the incident wave packet.Notably,the introduction of spin angular momentum into the wave packet induces spin-orbit coupling,resulting in a distinctive tilt of the STOV array.These findings open potential pathways for high-dimensional optical manipulation and advanced optical information multiplexing.
文摘为了解决森林火灾、盗伐树木和伤害野生动物等林业管理方面的问题,设计了基于ROS的智能林业巡逻机器人。该机器人采用Raspberry Pi 4B作为主控、STM32F103RCT6拓展板作为辅控,主要硬件采用深度相机、激光雷达等,多种传感器协同对各种复杂环境进行环境感知。系统使用Ubuntu18.04,在电脑终端利用VNC远程控制平台对机器人进行操控。机器人具备自主巡逻、实时监控和物体识别等功能,并选用Cartographer建图算法进行自主导航,利用深度学习算法YOLOv4-tiny进行物体识别。实验测试结果表明,智能林业巡逻机器人运行稳定,可有效解决林业管理的问题,具有很高的实际应用价值。
基金supported by research grants from the Guangdong Province Basic and Applied Basic Research Fund Project(2022A1515110447)Open Fund Project of the State Key Laboratory of Applied Microbiology in South China(SKLAM006-2022)+1 种基金74th batch of general funding from the China Postdoctoral Science Foundation(2023M740774)Guangdong Provincial People’s Hospital,Postdoctoral Research Launch Fund(BY012022017)。
文摘Epidemiological studies have indicated that branched-chain amino acids(BCAAs)increased and gut microbiota disordered in type 2 diabetes mellitus(T2DM).This study aimed to investigate the mechanism of Lactiplantibacillus plantarum strain 84-3(Lp84-3)combined with Staphylococcus aureus bacteriophage on ameliorating T2DM.Here we perform a case-control study and identify that Staphylococcus_phage was inversely correlated with fasting blood glucose(FBG).It revealed that Lp84-3 could inhibit the growth of S.aureus,and Lp84-3 contains BCAAs degradation enzymes in its genome.Furthermore,Lp84-3 alone or combined with S.aureus bacteriophage interventions can improve blood glucose,insulin resistance,triglycerides,interleukin-1β,tumor necrosis factor-α(TNF-α),BCAAs,and acetyllactate synthase(ALS)in db/db mice.Lp84-3 and S.aureus bacteriophage decreased S.aureus,Malacoplasma iowae,and Oscillibacter sp.,and increased some beneficial such as L.plantarum and Muribaculaceae bacterium.Transcriptomic analyses revealed that Lp84-3 and S.aureus bacteriophage activated the PI3K/AKT/GLUT4 signaling pathway and upregulated key genes of Il22,Hgf,Col6a1,Gh,Itga10,Fgf23,and Prl involved in glucose metabolism in hypothalamus.Collectively,Lp84-3 and S.aureus bacteriophage alleviate T2DM by modulating gut microbiota and enhancing glucose metabolism in hypothalamus,supporting its potential use as a promising functional compound microecological agent for alleviating T2DM.
文摘针对大型气象监测站成本高、覆盖范围有限、灵活性差的问题,设计了一套面向局部定点区域的智能化微型气象监测系统。首先,基于树莓派Raspberry Pi 4B平台开发了一套微型气象数据采集装置,可实现多种气象参数的实时采集与传输。然后,基于Qt开发了一款服务端软件,实现了针对前端采集气象数据的接收、存储、管理和可视化显示。前端采集系统实现了多种气象信息的综合检测,且系统功能可靠稳定;服务端系统实现的大数据管理和图形化显示,增强了系统可读性和实用性。该系统不仅能为气象数据的联合分析提供可靠数据源,也为业界开发相关商业产品提供了借鉴思路。
