Background: The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor;plasma PAI-1 level is high in type 2 diabetes. 4G/5G polymorphism of PAI-1 gene is a major genetic determinant of plasma ...Background: The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor;plasma PAI-1 level is high in type 2 diabetes. 4G/5G polymorphism of PAI-1 gene is a major genetic determinant of plasma PAI-1 levels, with 4G carriers having high PAI-1 level than 5G, theses pose the question about relation T2 patients and those polymorphisms. The aim of this study was to determine the relationship between the polymorphisms −675 4G/5G and −844 G/A of PAI-1 gene and type 2 diabetes mellitus. Methods: A case control study of 491 diabetic and 400 healthy controls. Genotyping of the polymorphism −675 4G/5G was done by PCR-ASA (polymerase chain reaction, allele specific amplification), and the polymorphism −844 G/A was done with PCR-RFLP (restriction fragment length polymorphism), the allelic frequency is calculated with hardy-Weinberg law, the statistic analysis was done by SPSS version 10. Results: Higher frequencies of The genotypes 4G/4G (p = 0.01) and 4G/5G of polymorphism −675 4G/5G were seen in diabetic (p = 0.05) and higher frequencies of 5G/5G was seen in controls (p −844 G/A was seen in diabetics and G/G was seen in controls (p = 0.01). Conclusion: Our study found association between 4G allele of −675 4G/5G and A allele of −844 G/A of PAI-1 gene and having type 2 diabetes mellitus in Tunisian population.展开更多
目的探讨脑小血管病(CSVD)患者外周血G蛋白耦联雌激素受体30(GPER30)、神经元PAS结构域蛋白4(NPAS4)、FK506结合蛋白5(FKBP5)表达与认知功能障碍(CD)的相关性。方法前瞻性选取2022年1月至2023年12月郑州大学第二附属医院收治的227例CSV...目的探讨脑小血管病(CSVD)患者外周血G蛋白耦联雌激素受体30(GPER30)、神经元PAS结构域蛋白4(NPAS4)、FK506结合蛋白5(FKBP5)表达与认知功能障碍(CD)的相关性。方法前瞻性选取2022年1月至2023年12月郑州大学第二附属医院收治的227例CSVD患者,根据有无CD分为障碍组(n=66)与无障碍组(n=161)。比较两组患者的一般资料及外周血GPER30、NPAS4、FKBP5 m RNA表达水平,Logistic回归分析CSVD患者CD的影响因素,比较不同程度CD患者外周血GPER30、NPAS4、FKBP5 m RNA表达水平,采用Pearson法分析外周血GPER30、NPAS4、FKBP5 m RNA表达与蒙特利尔认知评估量表(Mo CA)评分的相关性。结果障碍组患者的年龄、病程分别为(72.49±5.68)岁、(2.69±0.78)年,明显高(长)于无障碍组的(67.51±7.04)岁、(2.31±0.62)年,差异均有统计学意义(P<0.05);障碍组患者的外周血GPER30 m RNA表达水平为1.02±0.17,明显低于无障碍组的1.66±0.31,NPAS4m RNA、FKBP5 m RNA表达水平分别为2.79±0.60、3.88±1.12,明显高于无障碍组的1.55±0.51、2.10±0.59,差异均有统计学意义(P<0.05);Logistic回归分析结果显示,年龄、病程、GPER30 m RNA、NPAS4 m RNA及FKBP5 m RNA均为CSVD患者CD的独立影响因素(P<0.05)。轻度组患者的外周血GPER30 m RNA表达水平为1.27±0.25,明显高于中重度组的0.70±0.12,NPAS4 m RNA、FKBP5 m RNA表达水平分别为2.31±0.58、3.19±1.07,明显低于中重度组的3.40±0.72、4.76±1.39,差异均有统计学意义(P<0.05);Pearson法分析结果显示,外周血GPER30 m RNA表达与CSVD患者Mo CA评分呈正相关(r=0.704,P<0.05),NPAS4 m RNA、FKBP5 m RNA与Mo CA评分呈负相关(r=-0.572、-0.542,P<0.05)。结论外周血GPER30、NPAS4、FKBP5是CSVD患者CD的独立相关因素,各指标表达水平与CD病情严重程度均具有一定相关性,可为临床判断CD、评估CD病情严重程度提供参考,以指导后续临床工作。展开更多
随着4G/5G网络的快速发展,4G/5G网络覆盖的深度和广度有待完善,部分区域存在弱覆盖或盲区,针对网络覆盖建设周期长、成本高的问题。本文首先分析快速解决4G/5G网络弱覆盖存在的问题,其次围绕怎样实现弱覆盖或盲点区域快速覆盖4G/5G网络...随着4G/5G网络的快速发展,4G/5G网络覆盖的深度和广度有待完善,部分区域存在弱覆盖或盲区,针对网络覆盖建设周期长、成本高的问题。本文首先分析快速解决4G/5G网络弱覆盖存在的问题,其次围绕怎样实现弱覆盖或盲点区域快速覆盖4G/5G网络开展研究,提出利旧小区宽带空闲的传输网络资源连接微功率射频拉远单元(pico remote radio unit,PRRU)的方案,该方案可实现4G/5G网络弱覆盖快速处理。最后对方案的实用性及有效性进行了阐述。本研究为快速解决4G/5G网络弱覆盖问题提供了新思路和新方法。展开更多
文摘Background: The plasminogen activator inhibitor-1 (PAI-1) is a puissant antifibrinolytic factor;plasma PAI-1 level is high in type 2 diabetes. 4G/5G polymorphism of PAI-1 gene is a major genetic determinant of plasma PAI-1 levels, with 4G carriers having high PAI-1 level than 5G, theses pose the question about relation T2 patients and those polymorphisms. The aim of this study was to determine the relationship between the polymorphisms −675 4G/5G and −844 G/A of PAI-1 gene and type 2 diabetes mellitus. Methods: A case control study of 491 diabetic and 400 healthy controls. Genotyping of the polymorphism −675 4G/5G was done by PCR-ASA (polymerase chain reaction, allele specific amplification), and the polymorphism −844 G/A was done with PCR-RFLP (restriction fragment length polymorphism), the allelic frequency is calculated with hardy-Weinberg law, the statistic analysis was done by SPSS version 10. Results: Higher frequencies of The genotypes 4G/4G (p = 0.01) and 4G/5G of polymorphism −675 4G/5G were seen in diabetic (p = 0.05) and higher frequencies of 5G/5G was seen in controls (p −844 G/A was seen in diabetics and G/G was seen in controls (p = 0.01). Conclusion: Our study found association between 4G allele of −675 4G/5G and A allele of −844 G/A of PAI-1 gene and having type 2 diabetes mellitus in Tunisian population.
文摘目的探讨脑小血管病(CSVD)患者外周血G蛋白耦联雌激素受体30(GPER30)、神经元PAS结构域蛋白4(NPAS4)、FK506结合蛋白5(FKBP5)表达与认知功能障碍(CD)的相关性。方法前瞻性选取2022年1月至2023年12月郑州大学第二附属医院收治的227例CSVD患者,根据有无CD分为障碍组(n=66)与无障碍组(n=161)。比较两组患者的一般资料及外周血GPER30、NPAS4、FKBP5 m RNA表达水平,Logistic回归分析CSVD患者CD的影响因素,比较不同程度CD患者外周血GPER30、NPAS4、FKBP5 m RNA表达水平,采用Pearson法分析外周血GPER30、NPAS4、FKBP5 m RNA表达与蒙特利尔认知评估量表(Mo CA)评分的相关性。结果障碍组患者的年龄、病程分别为(72.49±5.68)岁、(2.69±0.78)年,明显高(长)于无障碍组的(67.51±7.04)岁、(2.31±0.62)年,差异均有统计学意义(P<0.05);障碍组患者的外周血GPER30 m RNA表达水平为1.02±0.17,明显低于无障碍组的1.66±0.31,NPAS4m RNA、FKBP5 m RNA表达水平分别为2.79±0.60、3.88±1.12,明显高于无障碍组的1.55±0.51、2.10±0.59,差异均有统计学意义(P<0.05);Logistic回归分析结果显示,年龄、病程、GPER30 m RNA、NPAS4 m RNA及FKBP5 m RNA均为CSVD患者CD的独立影响因素(P<0.05)。轻度组患者的外周血GPER30 m RNA表达水平为1.27±0.25,明显高于中重度组的0.70±0.12,NPAS4 m RNA、FKBP5 m RNA表达水平分别为2.31±0.58、3.19±1.07,明显低于中重度组的3.40±0.72、4.76±1.39,差异均有统计学意义(P<0.05);Pearson法分析结果显示,外周血GPER30 m RNA表达与CSVD患者Mo CA评分呈正相关(r=0.704,P<0.05),NPAS4 m RNA、FKBP5 m RNA与Mo CA评分呈负相关(r=-0.572、-0.542,P<0.05)。结论外周血GPER30、NPAS4、FKBP5是CSVD患者CD的独立相关因素,各指标表达水平与CD病情严重程度均具有一定相关性,可为临床判断CD、评估CD病情严重程度提供参考,以指导后续临床工作。
文摘随着4G/5G网络的快速发展,4G/5G网络覆盖的深度和广度有待完善,部分区域存在弱覆盖或盲区,针对网络覆盖建设周期长、成本高的问题。本文首先分析快速解决4G/5G网络弱覆盖存在的问题,其次围绕怎样实现弱覆盖或盲点区域快速覆盖4G/5G网络开展研究,提出利旧小区宽带空闲的传输网络资源连接微功率射频拉远单元(pico remote radio unit,PRRU)的方案,该方案可实现4G/5G网络弱覆盖快速处理。最后对方案的实用性及有效性进行了阐述。本研究为快速解决4G/5G网络弱覆盖问题提供了新思路和新方法。
