To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied Methods RNA was e...To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied Methods RNA was extracted from the selected tumor areas of formalin fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT PCR) and Southern blotting Results Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma A high positive rate was found in Chinese patients (83%) as compared to US patients (33%) Conclusion Our finding suggests HCV may play a role in the malignant transformation of bile duct cells展开更多
文摘To investigate the role of hepatitis C virus (HCV) in the malignant transformation of bile duct cells Tissues from 6 Chinese patients and 6 American patients with cholangiocarcinoma were studied Methods RNA was extracted from the selected tumor areas of formalin fixed, paraffin embedded sections, followed by reverse transcription double polymerase chain reaction (RT PCR) and Southern blotting Results Positive and negative strand HCV RNA sequences were detected in seven out of twelve patients with cholangiocarcinoma A high positive rate was found in Chinese patients (83%) as compared to US patients (33%) Conclusion Our finding suggests HCV may play a role in the malignant transformation of bile duct cells
