The genome tagging project(GTP)plays a pivotal role in addressing a critical gap in the understanding of protein functions.Within this framework,we successfully generated a human influenza hemagglutinin-tagged sperm-s...The genome tagging project(GTP)plays a pivotal role in addressing a critical gap in the understanding of protein functions.Within this framework,we successfully generated a human influenza hemagglutinin-tagged sperm-specific protein 411(HA-tagged Ssp411)mouse model.This model is instrumental in probing the expression and function of Ssp411.Our research revealed that Ssp411 is expressed in the round spermatids,elongating spermatids,elongated spermatids,and epididymal spermatozoa.The comprehensive examination of the distribution of Ssp411 in these germ cells offers new perspectives on its involvement in spermiogenesis.Nevertheless,rigorous further inquiry is imperative to elucidate the precise mechanistic underpinnings of these functions.Ssp411 is not detectable in metaphase Ⅱ(MⅡ)oocytes,zygotes,or 2-cell stage embryos,highlighting its intricate role in early embryonic development.These findings not only advance our understanding of the role of Ssp411 in reproductive physiology but also significantly contribute to the overarching goals of the GTP,fostering groundbreaking advancements in the f ields of spermiogenesis and reproductive biology.展开更多
The melting and solidification process of DZ411 superalloy at different cooling rates(50,200,500°C/min)was observed in situ by high-temperature confocal laser scanning microscopy.The solidification behaviour of t...The melting and solidification process of DZ411 superalloy at different cooling rates(50,200,500°C/min)was observed in situ by high-temperature confocal laser scanning microscopy.The solidification behaviour of this alloy was also studied through other methods such as differential scanning calorimetry and scanning electron microscopy.The results show that the precipitation sequence of the main phases during the solidification of DZ411 alloy isγmatrix phase,carbide phase and Laves phase.Besides,during the solidification process of DZ411 alloy,both the dendrite thickness and dendrite spacing decreased with the increasing of cooling rate.In addition,a large amount of Ta is enriched in the dendrite stems at the end of solidification,which is the main reason for the formation of Laves phase.As the cooling rate increases,the size of the Laves phase becomes smaller and the distribution becomes more dispersed,which effectively inhibits the segregation of the alloy.展开更多
目的对Roche cobas e 411全自动化学发光分析仪进行性能验证。方法对分析系统进行精密度、准确度、线性试验及携带污染率等指标测试。结果批内精密度CV<4%,批间精密度CV<5%,携带污染率0.13%,线性回归系数0.99。结论 Roche cobas e...目的对Roche cobas e 411全自动化学发光分析仪进行性能验证。方法对分析系统进行精密度、准确度、线性试验及携带污染率等指标测试。结果批内精密度CV<4%,批间精密度CV<5%,携带污染率0.13%,线性回归系数0.99。结论 Roche cobas e 411全自动化学发光分析仪具有良好的线性、准确性和重复性,是临床实验室较理想的免疫分析仪器。展开更多
基金the support from the National Natural Science Foundation of China(No.32070849)The Foundation of Science and Technology Commission of Shanghai Municipality(No.22DX1900400)+1 种基金Science and Technology Commission of Shanghai Municipality(No.23JC1403803)Shanghai Municipal Science and Technology Commission Targeted Funding Project(No.22DX1900400).
文摘The genome tagging project(GTP)plays a pivotal role in addressing a critical gap in the understanding of protein functions.Within this framework,we successfully generated a human influenza hemagglutinin-tagged sperm-specific protein 411(HA-tagged Ssp411)mouse model.This model is instrumental in probing the expression and function of Ssp411.Our research revealed that Ssp411 is expressed in the round spermatids,elongating spermatids,elongated spermatids,and epididymal spermatozoa.The comprehensive examination of the distribution of Ssp411 in these germ cells offers new perspectives on its involvement in spermiogenesis.Nevertheless,rigorous further inquiry is imperative to elucidate the precise mechanistic underpinnings of these functions.Ssp411 is not detectable in metaphase Ⅱ(MⅡ)oocytes,zygotes,or 2-cell stage embryos,highlighting its intricate role in early embryonic development.These findings not only advance our understanding of the role of Ssp411 in reproductive physiology but also significantly contribute to the overarching goals of the GTP,fostering groundbreaking advancements in the f ields of spermiogenesis and reproductive biology.
基金funded by the Key Science and Technology Projects of Gansu Province(Grant No.252DGA006)Central Fund for Guiding Local Science and Technology Development Projects(Grant No.25ZYJB001)+3 种基金Gansu Key Research and Development Project(Grant No.23YFGA0003)Gansu Provincial Joint Research Fund(Grant No.24JRRB004)the Industry support plan of Gansu Universities(Grant No.2024CYZC-01)the Science and Technology Projects of Jiayuguan City(No.24-10,QKJ24-01,QKJ24-10).
文摘The melting and solidification process of DZ411 superalloy at different cooling rates(50,200,500°C/min)was observed in situ by high-temperature confocal laser scanning microscopy.The solidification behaviour of this alloy was also studied through other methods such as differential scanning calorimetry and scanning electron microscopy.The results show that the precipitation sequence of the main phases during the solidification of DZ411 alloy isγmatrix phase,carbide phase and Laves phase.Besides,during the solidification process of DZ411 alloy,both the dendrite thickness and dendrite spacing decreased with the increasing of cooling rate.In addition,a large amount of Ta is enriched in the dendrite stems at the end of solidification,which is the main reason for the formation of Laves phase.As the cooling rate increases,the size of the Laves phase becomes smaller and the distribution becomes more dispersed,which effectively inhibits the segregation of the alloy.
文摘目的对Roche cobas e 411全自动化学发光分析仪进行性能验证。方法对分析系统进行精密度、准确度、线性试验及携带污染率等指标测试。结果批内精密度CV<4%,批间精密度CV<5%,携带污染率0.13%,线性回归系数0.99。结论 Roche cobas e 411全自动化学发光分析仪具有良好的线性、准确性和重复性,是临床实验室较理想的免疫分析仪器。
