Objective Y-Chromosomal short tandem repeat polymorphism(Y-STR)analysis plays an indispensable role in the identification of male individuals,population genetics,and biogeographic research.While profiles of many popul...Objective Y-Chromosomal short tandem repeat polymorphism(Y-STR)analysis plays an indispensable role in the identification of male individuals,population genetics,and biogeographic research.While profiles of many populations based on Y-STR markers in human genomes are ample,haplotype data for the Wuwei Han are still scarce.Methods In this study,2180 unrelated Wuwei Han male individuals residing in Gansu Province,China were collected and genotyped using the novel Microreader™40Y Plus ID system.Phylogenetic relationship reconstructions,multidimensional scaling(MDS),and heatmap analysis were performed based on the genetic distance(Rst)values between our studied population and other populations of the Ymax module in the Y-STR Haplotype Reference Database(YHRD).Results A total of 2129 unique haplotypes were obtained,and the haplotype diversity(HD)and discrimination capacity(DC)for the Wuwei Han were 0.9999 and 0.9931,respectively.Conclusion Our results demonstrate that the Wuwei Han population had intimate genetic relationships with East Asians,especially the geographically close Han populations.Overall,this Y-Chromosomal assay gives valuable information about paternal lineages in male individual tracking and genealogical database construction.展开更多
The purpose of this paper is double： first to measure the natural radioactivity in any food by detecting the omnipresent radiation of ^40K, and then to use this date to obtain the concentration of the important miner...The purpose of this paper is double： first to measure the natural radioactivity in any food by detecting the omnipresent radiation of ^40K, and then to use this date to obtain the concentration of the important mineral nutrient K, by dividing the specific activity of the sample （Bq/g） by the specific activity of elementary K （31.19 Bq/g）. The method implies the γ rays detection emitted by natural ^40K-^40Ar present in foodstuff, by using a 3 × 3＂ low background NaI （TI） scintillation detector coupled to a PC charged with the Maestro Program. When this detection system is calibrated in order to determine its efficiency for 1461 KeV γrays emitted during ^40K-^40Ar decaying, it is possible to measure easily and with enough precision the specific activity due to natural ^40K present in any foodstuff. Once this figure is divided by the constant value of ^40K specific activity of elementary K, the concentration of this element in the foodstuff sample is established. This paper describes the procedure and shows the results obtained with four basic diet products： milk （powdered, light and cream）, eggs （yellow and white）, wheat （whole and refined flour） and corn （whole and cornstarch flour）. The possible application of this study seems to be to establish the necessary threshold to evaluate the importance of any possible radioactive contamination, as well as to measure the concentration of the mineral nutrient K in foodstuff, by means of an easy, practicable method. Also, it might be useful as an experiment for teaching purposes.展开更多
基金supported by grants from the Shaanxi Basic Research Program of Natural Science(No.2021JQ-392)the International Cooperation Project of Shaanxi Province(No.2021KW-47).
文摘Objective Y-Chromosomal short tandem repeat polymorphism(Y-STR)analysis plays an indispensable role in the identification of male individuals,population genetics,and biogeographic research.While profiles of many populations based on Y-STR markers in human genomes are ample,haplotype data for the Wuwei Han are still scarce.Methods In this study,2180 unrelated Wuwei Han male individuals residing in Gansu Province,China were collected and genotyped using the novel Microreader™40Y Plus ID system.Phylogenetic relationship reconstructions,multidimensional scaling(MDS),and heatmap analysis were performed based on the genetic distance(Rst)values between our studied population and other populations of the Ymax module in the Y-STR Haplotype Reference Database(YHRD).Results A total of 2129 unique haplotypes were obtained,and the haplotype diversity(HD)and discrimination capacity(DC)for the Wuwei Han were 0.9999 and 0.9931,respectively.Conclusion Our results demonstrate that the Wuwei Han population had intimate genetic relationships with East Asians,especially the geographically close Han populations.Overall,this Y-Chromosomal assay gives valuable information about paternal lineages in male individual tracking and genealogical database construction.
文摘The purpose of this paper is double： first to measure the natural radioactivity in any food by detecting the omnipresent radiation of ^40K, and then to use this date to obtain the concentration of the important mineral nutrient K, by dividing the specific activity of the sample （Bq/g） by the specific activity of elementary K （31.19 Bq/g）. The method implies the γ rays detection emitted by natural ^40K-^40Ar present in foodstuff, by using a 3 × 3＂ low background NaI （TI） scintillation detector coupled to a PC charged with the Maestro Program. When this detection system is calibrated in order to determine its efficiency for 1461 KeV γrays emitted during ^40K-^40Ar decaying, it is possible to measure easily and with enough precision the specific activity due to natural ^40K present in any foodstuff. Once this figure is divided by the constant value of ^40K specific activity of elementary K, the concentration of this element in the foodstuff sample is established. This paper describes the procedure and shows the results obtained with four basic diet products： milk （powdered, light and cream）, eggs （yellow and white）, wheat （whole and refined flour） and corn （whole and cornstarch flour）. The possible application of this study seems to be to establish the necessary threshold to evaluate the importance of any possible radioactive contamination, as well as to measure the concentration of the mineral nutrient K in foodstuff, by means of an easy, practicable method. Also, it might be useful as an experiment for teaching purposes.
