(E)-4-Hydroxyhexenal(HHE)is receiving widespread attention as a representative reactive carbonyl species(RCS)derived from n-3 polyunsaturated fatty acids peroxidation due to its health hazard.However,few studies have ...(E)-4-Hydroxyhexenal(HHE)is receiving widespread attention as a representative reactive carbonyl species(RCS)derived from n-3 polyunsaturated fatty acids peroxidation due to its health hazard.However,few studies have addressed the potential effects of lipid oxidation products on microbial growth in food products.Therefore,this study attempted to elucidate the action of HHE on Lactiplantibacillus plantarum(L.plantarum),the dominant bacteria in dry-cured fish.The results showed that the minimum inhibitory concentration(MIC)of HHE was 250μg/mL.Time-kill curves confirmed the strong inhibitory activity of HHE.Lactate dehydrogenase(LDH)assay indicated that HHE disrupted the integrity of cell wall.Scanning electron microscopy(SEM)and flow cytometry analysis revealed that HHE induced changes in cell morphology,compromised cell membrane permeability,and thus disrupted intracellular homeostasis.Based on untargeted metabolomic analysis,HHE disrupted multiple metabolic pathways in L.plantarum involving amino acid metabolism,lipid metabolism,protein biosynthesis,nucleic acid metabolism and energy metabolism.This research provide new insights into the HHE inhibition mechanism and theoretical support for studying the effects of lipid oxidation products on microorganisms in food matrix.展开更多
采用Co_(3)O_(4)吸附脱除模拟柴油中的喹啉、吡啶或苯胺,考察了最佳吸附温度、吸附时间等条件,同时进行了吸附热力学和动力学研究;基于第一性原理对Co_(3)O_(4)晶胞进行相分析,对3种氮化物进行最高占据分子轨道(HOMO)-最低未占据分子轨...采用Co_(3)O_(4)吸附脱除模拟柴油中的喹啉、吡啶或苯胺,考察了最佳吸附温度、吸附时间等条件,同时进行了吸附热力学和动力学研究;基于第一性原理对Co_(3)O_(4)晶胞进行相分析,对3种氮化物进行最高占据分子轨道(HOMO)-最低未占据分子轨道(LUMO)分析,计算了吸附构型的吸附能和最稳定吸附构型的Mulliken电荷转移与电子密度。结果表明:在15 mL模拟柴油中加入0.6 g Co_(3)O_(4),苯胺、吡啶、喹啉的最佳吸附温度分别为20、20和30℃,最佳吸附时间分别为30、30、40 min,吸附容量由大到小顺序均为苯胺>吡啶>喹啉。热力学与动力学分析表明,喹啉、吡啶、苯胺的吸附均更符合多分子层吸附的Freundlich模型和准二级动力学方程。HOMO-LUMO分析结果表明,Co_(3)O_(4)为电子接受体,3种氮化物为电子给予体,Co_(3)O_(4)对喹啉、吡啶的配位吸附结构最稳定,对苯胺的π络合吸附最稳定。电荷转移计算表明,苯胺、吡啶、喹啉向Co_(3)O_(4)团簇转移的电荷数分别为0.423、0.394、0.368,说明Co_(3)O_(4)吸附3种氮化物的吸附能力大小为苯胺>吡啶>喹啉;电子密度图结果表明,最稳定吸附结构中Co_(3)O_(4)与3种氮化物均形成了化学键。展开更多
基金supported financially by the National Natural Science Foundation of China(No.31871869)the National Key R&D Pro-gram of China(No.2018YFD0901006).
文摘(E)-4-Hydroxyhexenal(HHE)is receiving widespread attention as a representative reactive carbonyl species(RCS)derived from n-3 polyunsaturated fatty acids peroxidation due to its health hazard.However,few studies have addressed the potential effects of lipid oxidation products on microbial growth in food products.Therefore,this study attempted to elucidate the action of HHE on Lactiplantibacillus plantarum(L.plantarum),the dominant bacteria in dry-cured fish.The results showed that the minimum inhibitory concentration(MIC)of HHE was 250μg/mL.Time-kill curves confirmed the strong inhibitory activity of HHE.Lactate dehydrogenase(LDH)assay indicated that HHE disrupted the integrity of cell wall.Scanning electron microscopy(SEM)and flow cytometry analysis revealed that HHE induced changes in cell morphology,compromised cell membrane permeability,and thus disrupted intracellular homeostasis.Based on untargeted metabolomic analysis,HHE disrupted multiple metabolic pathways in L.plantarum involving amino acid metabolism,lipid metabolism,protein biosynthesis,nucleic acid metabolism and energy metabolism.This research provide new insights into the HHE inhibition mechanism and theoretical support for studying the effects of lipid oxidation products on microorganisms in food matrix.
文摘采用Co_(3)O_(4)吸附脱除模拟柴油中的喹啉、吡啶或苯胺,考察了最佳吸附温度、吸附时间等条件,同时进行了吸附热力学和动力学研究;基于第一性原理对Co_(3)O_(4)晶胞进行相分析,对3种氮化物进行最高占据分子轨道(HOMO)-最低未占据分子轨道(LUMO)分析,计算了吸附构型的吸附能和最稳定吸附构型的Mulliken电荷转移与电子密度。结果表明:在15 mL模拟柴油中加入0.6 g Co_(3)O_(4),苯胺、吡啶、喹啉的最佳吸附温度分别为20、20和30℃,最佳吸附时间分别为30、30、40 min,吸附容量由大到小顺序均为苯胺>吡啶>喹啉。热力学与动力学分析表明,喹啉、吡啶、苯胺的吸附均更符合多分子层吸附的Freundlich模型和准二级动力学方程。HOMO-LUMO分析结果表明,Co_(3)O_(4)为电子接受体,3种氮化物为电子给予体,Co_(3)O_(4)对喹啉、吡啶的配位吸附结构最稳定,对苯胺的π络合吸附最稳定。电荷转移计算表明,苯胺、吡啶、喹啉向Co_(3)O_(4)团簇转移的电荷数分别为0.423、0.394、0.368,说明Co_(3)O_(4)吸附3种氮化物的吸附能力大小为苯胺>吡啶>喹啉;电子密度图结果表明,最稳定吸附结构中Co_(3)O_(4)与3种氮化物均形成了化学键。
