Enhanced inflammatory response and oxidative stress cause acute lung injury(ALI). Controlling inflammation and oxidation can ameliorate ALI. In the present study, we aimed to determine whether 3,4-Dihydroxyacetophenon...Enhanced inflammatory response and oxidative stress cause acute lung injury(ALI). Controlling inflammation and oxidation can ameliorate ALI. In the present study, we aimed to determine whether 3,4-Dihydroxyacetophenone(compound 1)could ameliorate lipopolysaccharide(LPS)-induced ALI by suppressing inflammation and oxidation. In this study, compound 1 reduced LPS-induced inflammatory cytokines and oxidative stress in RAW 264.7 cells. Moreover, compound 1 suppressed the expression of inflammatory protein p65, inhibited IkBα phosphorylation, decreased the nuclear translocation of p65, and increased the expressions of anti-oxidative protein nuclear factor erythroid 2-related factor 2(Nrf-2) and heme oxygenase-1(HO-1), which was reduced by LPS, in leukemia cells in mouse macrophage(RAW 264.7) cells. Furthermore, compound 1 could also ameliorate LPS-induced ALI in vivo, with a reduction of inflammatory cytokines, oxidative stress, and nuclear factor-kappa B(NF-κB)signaling pathway activation. This study emphasized the anti-inflammatory and anti-oxidative activities of compound 1, which could be a valuable therapeutic agent against ALI.展开更多
A new fluorescent reagent 2,4-dihydroxyacetophenone benzoylhydrazone （DHAPBH） was synthesized and its ionization constants were established spectrophotometrically. The fluorescent reaction of this reagent with cadmi...A new fluorescent reagent 2,4-dihydroxyacetophenone benzoylhydrazone （DHAPBH） was synthesized and its ionization constants were established spectrophotometrically. The fluorescent reaction of this reagent with cadmium was studied. The cadmium ion can form a stable binary chelate with DHAPBH, having a mole ratio of 1 ：2 in the pH range of 3.6-4.4. Based on this chelation reaction, a highly sensitive spectrofiuorimetric method was developed for determination of trace amounts of cadmium in a water-ethanol （7：3 in volume ratio） medium at pH = 4.0. Under these conditions, the Cd-DHAPBH complex has excitation and emission maxima at 306 and 437 nm, respectively. The linear range of the method is from 0.36 to 140μtg·L^-1 and the detection limit is 0.27 μtg·L^-1 of cadmium. Interferences of other ions were studied. The method was successfully applied to the determination of cadmium in mineral water, tap water, fiver water, and wastewater.展开更多
基金National Natural Science Foundation of China (Grant No. 82003755)the Medical Technology Program of Ningbo (Grant No. 2019Y07)。
文摘Enhanced inflammatory response and oxidative stress cause acute lung injury(ALI). Controlling inflammation and oxidation can ameliorate ALI. In the present study, we aimed to determine whether 3,4-Dihydroxyacetophenone(compound 1)could ameliorate lipopolysaccharide(LPS)-induced ALI by suppressing inflammation and oxidation. In this study, compound 1 reduced LPS-induced inflammatory cytokines and oxidative stress in RAW 264.7 cells. Moreover, compound 1 suppressed the expression of inflammatory protein p65, inhibited IkBα phosphorylation, decreased the nuclear translocation of p65, and increased the expressions of anti-oxidative protein nuclear factor erythroid 2-related factor 2(Nrf-2) and heme oxygenase-1(HO-1), which was reduced by LPS, in leukemia cells in mouse macrophage(RAW 264.7) cells. Furthermore, compound 1 could also ameliorate LPS-induced ALI in vivo, with a reduction of inflammatory cytokines, oxidative stress, and nuclear factor-kappa B(NF-κB)signaling pathway activation. This study emphasized the anti-inflammatory and anti-oxidative activities of compound 1, which could be a valuable therapeutic agent against ALI.
基金This work was financially supported by the Educational Commission of Gansu Province, China (No. 029-04)
文摘A new fluorescent reagent 2,4-dihydroxyacetophenone benzoylhydrazone （DHAPBH） was synthesized and its ionization constants were established spectrophotometrically. The fluorescent reaction of this reagent with cadmium was studied. The cadmium ion can form a stable binary chelate with DHAPBH, having a mole ratio of 1 ：2 in the pH range of 3.6-4.4. Based on this chelation reaction, a highly sensitive spectrofiuorimetric method was developed for determination of trace amounts of cadmium in a water-ethanol （7：3 in volume ratio） medium at pH = 4.0. Under these conditions, the Cd-DHAPBH complex has excitation and emission maxima at 306 and 437 nm, respectively. The linear range of the method is from 0.36 to 140μtg·L^-1 and the detection limit is 0.27 μtg·L^-1 of cadmium. Interferences of other ions were studied. The method was successfully applied to the determination of cadmium in mineral water, tap water, fiver water, and wastewater.
