AIM:To examine the effects of 2,4-dihydroxybenzophenone(BP-1),a benzophenone derivative used as an ultraviolet light absorbent,on acetaminophen(APAP)induced hepatotoxicity in C57BL/6J mice.METHODS:Mice were administer...AIM:To examine the effects of 2,4-dihydroxybenzophenone(BP-1),a benzophenone derivative used as an ultraviolet light absorbent,on acetaminophen(APAP)induced hepatotoxicity in C57BL/6J mice.METHODS:Mice were administered orally with BP-1 at doses of 200,400 and 800 mg/kg body weight respectively every morning for 4 d before a hepatotoxic dose of APAP(350 mg/kg body weight) was given subcutaneously.Twenty four hours after APAP intoxication,the serum enzyme including serum alaine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH) were measured and liver histopathologic changes were examined.RESULTS:BP-1 administration dramatically reduced serum ALT,AST and LDH levels.Liver histopathological examination showed that BP-1 administration antagonized APAP-induced liver pathological damage in a dose-dependent manner.Further tests showed that APAP-induced hepatic lipid peroxidation was reduced significantly by BP-1 pretreatment,and glutathione depletion was ameliorated obviously.CONCLUSION:BP-1 can effectively protect C57BL/6J mice from APAP-induced hepatotoxicity,and reduction of oxidative stress might be part of the protection mechanism.展开更多
Enhanced inflammatory response and oxidative stress cause acute lung injury(ALI). Controlling inflammation and oxidation can ameliorate ALI. In the present study, we aimed to determine whether 3,4-Dihydroxyacetophenon...Enhanced inflammatory response and oxidative stress cause acute lung injury(ALI). Controlling inflammation and oxidation can ameliorate ALI. In the present study, we aimed to determine whether 3,4-Dihydroxyacetophenone(compound 1)could ameliorate lipopolysaccharide(LPS)-induced ALI by suppressing inflammation and oxidation. In this study, compound 1 reduced LPS-induced inflammatory cytokines and oxidative stress in RAW 264.7 cells. Moreover, compound 1 suppressed the expression of inflammatory protein p65, inhibited IkBα phosphorylation, decreased the nuclear translocation of p65, and increased the expressions of anti-oxidative protein nuclear factor erythroid 2-related factor 2(Nrf-2) and heme oxygenase-1(HO-1), which was reduced by LPS, in leukemia cells in mouse macrophage(RAW 264.7) cells. Furthermore, compound 1 could also ameliorate LPS-induced ALI in vivo, with a reduction of inflammatory cytokines, oxidative stress, and nuclear factor-kappa B(NF-κB)signaling pathway activation. This study emphasized the anti-inflammatory and anti-oxidative activities of compound 1, which could be a valuable therapeutic agent against ALI.展开更多
In the present study,we studied the effects of 3,4-dihydroxybenzaldehyde,derived from Salvia miltiorrhiza Bunge,on mouse sperm survival and sperm membrane integrity in vitro and on mouse reproductive damage induced by...In the present study,we studied the effects of 3,4-dihydroxybenzaldehyde,derived from Salvia miltiorrhiza Bunge,on mouse sperm survival and sperm membrane integrity in vitro and on mouse reproductive damage induced by cyclophosphamide in vivo.3,4-Dihydroxybenzaldehyde(0.01,0.1,and 1 mg/m L)improved sperm survival rate and sperm membrane integrity in separated mouse sperm(all P<0.05).In cyclophosphamide-treated male mice(60 mg/kg/d through intraperitoneal injection for 5 d),3,4-dihydroxybenzaldehyde(40 mg/kg/d through intragastric gavage for 35 d)increased the testis index,epididymis index,and sperm nuclear maturity(all P<0.05).3,4-Dihydroxybenzaldehyde also improved testis morphology characterized by orderly arranged layers of spermatogenic cells,numbers of sperm in the lumen,normal mesenchymal cells,and close and tidy arrangement of the seminiferous tubules.3,4-Dihydroxybenzaldehyde also increased testicular superoxide dismutase activity and elevated DJ-1 expression.In addition,it decreased the expression of ICAM-1 and enhanced the expressions of VCAM-1,PEDF,VEGF,and PPARγ.These findings indicated that 3,4-dihydroxybenzaldehyde increased mouse sperm survival and sperm membrane integrity in vitro and reduced mouse reproductive damage induced by cyclophosphamide via DJ-1 and other targets in vivo.3,4-Dihydroxybenzaldehyde might thus be useful for treating male reproductive damage and antineoplastic cyclophosphamide-induced reproductive toxicity.展开更多
The electrochemical oxidation of 1,4-dihydroxyanthraquinone has been studied in the presence of acetylacetone and benzoylacetone as nucleophiles in a mixture of ethanol/water by means of cyclic voltammetry as a diagno...The electrochemical oxidation of 1,4-dihydroxyanthraquinone has been studied in the presence of acetylacetone and benzoylacetone as nucleophiles in a mixture of ethanol/water by means of cyclic voltammetry as a diagnostic technique.The results indicate the participation of electrochemically produced anthraquinone in the Michael addition reaction with acetylacetone and benzoylacetone to form the corresponding new anthraquinone derivatives.On the basis of the EC mechanism,the observed homogeneous rate constants（kobs）of the reaction of anthraquinone with acetylacetone and benzoylacetone were estimated by comparing the experimental cyclic voltammograms with the digitally simulated results.展开更多
The conditions of color reaction of Aluminium(Ⅲ) with a new organic reagent 2,3,7 trihydroxy 9 [2,4 dihydroxy)phenylazo]phenylfluorone (ARPF) in the presence of cetyltrimethyammonium bromide(CTMAB) were studied....The conditions of color reaction of Aluminium(Ⅲ) with a new organic reagent 2,3,7 trihydroxy 9 [2,4 dihydroxy)phenylazo]phenylfluorone (ARPF) in the presence of cetyltrimethyammonium bromide(CTMAB) were studied. It was found that Aluminium(Ⅲ) reacts with ARPF and CTMAB in pH 6.5 HAc NaAc buffer solution to form a 1∶2 red complex with maximum absorption at 562 nm .The molar absorptivity is 1.10×10 5 L·mol -1 ·cm -1 . Beer's law is obeyed in the range of 0 5.5 μg Al(Ⅲ) per 25 mL. The method has been applied to determination of Aluminium in the limestone with satisfactory results.展开更多
Lipids are the basic components of cells that are involved in physiological processes such as energy transfer,signal transduction,cell growth,and apoptosis,thus playing important biological functions in organisms[1].M...Lipids are the basic components of cells that are involved in physiological processes such as energy transfer,signal transduction,cell growth,and apoptosis,thus playing important biological functions in organisms[1].Moreover,lipids are biomarkers for many diseases,including coronary heart disease,hepatitis C,and diabetes.A substantial number of studies have shown that obtaining accurate and comprehensive data on the spatial distribution of lipids is essential for investigating pathogenesis and identifying diagnostic biomarkers[2].展开更多
基金Supported by Drug Innovation Program of National Science and Technology Project, No. 2009ZX09103-007
文摘AIM:To examine the effects of 2,4-dihydroxybenzophenone(BP-1),a benzophenone derivative used as an ultraviolet light absorbent,on acetaminophen(APAP)induced hepatotoxicity in C57BL/6J mice.METHODS:Mice were administered orally with BP-1 at doses of 200,400 and 800 mg/kg body weight respectively every morning for 4 d before a hepatotoxic dose of APAP(350 mg/kg body weight) was given subcutaneously.Twenty four hours after APAP intoxication,the serum enzyme including serum alaine aminotransferase(ALT),aspartate aminotransferase(AST),lactate dehydrogenase(LDH) were measured and liver histopathologic changes were examined.RESULTS:BP-1 administration dramatically reduced serum ALT,AST and LDH levels.Liver histopathological examination showed that BP-1 administration antagonized APAP-induced liver pathological damage in a dose-dependent manner.Further tests showed that APAP-induced hepatic lipid peroxidation was reduced significantly by BP-1 pretreatment,and glutathione depletion was ameliorated obviously.CONCLUSION:BP-1 can effectively protect C57BL/6J mice from APAP-induced hepatotoxicity,and reduction of oxidative stress might be part of the protection mechanism.
基金National Natural Science Foundation of China (Grant No. 82003755)the Medical Technology Program of Ningbo (Grant No. 2019Y07)。
文摘Enhanced inflammatory response and oxidative stress cause acute lung injury(ALI). Controlling inflammation and oxidation can ameliorate ALI. In the present study, we aimed to determine whether 3,4-Dihydroxyacetophenone(compound 1)could ameliorate lipopolysaccharide(LPS)-induced ALI by suppressing inflammation and oxidation. In this study, compound 1 reduced LPS-induced inflammatory cytokines and oxidative stress in RAW 264.7 cells. Moreover, compound 1 suppressed the expression of inflammatory protein p65, inhibited IkBα phosphorylation, decreased the nuclear translocation of p65, and increased the expressions of anti-oxidative protein nuclear factor erythroid 2-related factor 2(Nrf-2) and heme oxygenase-1(HO-1), which was reduced by LPS, in leukemia cells in mouse macrophage(RAW 264.7) cells. Furthermore, compound 1 could also ameliorate LPS-induced ALI in vivo, with a reduction of inflammatory cytokines, oxidative stress, and nuclear factor-kappa B(NF-κB)signaling pathway activation. This study emphasized the anti-inflammatory and anti-oxidative activities of compound 1, which could be a valuable therapeutic agent against ALI.
基金Science and Technology Program of the Beijing Municipal Education Commission(Grant No.KM201810025002)National Natural Science Foundation of China(Grant No.81801522)Natural Science Foundation of Hebei Province(Grant No.H2018209342)。
文摘In the present study,we studied the effects of 3,4-dihydroxybenzaldehyde,derived from Salvia miltiorrhiza Bunge,on mouse sperm survival and sperm membrane integrity in vitro and on mouse reproductive damage induced by cyclophosphamide in vivo.3,4-Dihydroxybenzaldehyde(0.01,0.1,and 1 mg/m L)improved sperm survival rate and sperm membrane integrity in separated mouse sperm(all P<0.05).In cyclophosphamide-treated male mice(60 mg/kg/d through intraperitoneal injection for 5 d),3,4-dihydroxybenzaldehyde(40 mg/kg/d through intragastric gavage for 35 d)increased the testis index,epididymis index,and sperm nuclear maturity(all P<0.05).3,4-Dihydroxybenzaldehyde also improved testis morphology characterized by orderly arranged layers of spermatogenic cells,numbers of sperm in the lumen,normal mesenchymal cells,and close and tidy arrangement of the seminiferous tubules.3,4-Dihydroxybenzaldehyde also increased testicular superoxide dismutase activity and elevated DJ-1 expression.In addition,it decreased the expression of ICAM-1 and enhanced the expressions of VCAM-1,PEDF,VEGF,and PPARγ.These findings indicated that 3,4-dihydroxybenzaldehyde increased mouse sperm survival and sperm membrane integrity in vitro and reduced mouse reproductive damage induced by cyclophosphamide via DJ-1 and other targets in vivo.3,4-Dihydroxybenzaldehyde might thus be useful for treating male reproductive damage and antineoplastic cyclophosphamide-induced reproductive toxicity.
基金Bu-Ali Sina University Research Council and Center of Excellence in Development of Environmentally Friendly Methods for Chemical Synthesis(CEDEFMCS)for their supportto this work
文摘The electrochemical oxidation of 1,4-dihydroxyanthraquinone has been studied in the presence of acetylacetone and benzoylacetone as nucleophiles in a mixture of ethanol/water by means of cyclic voltammetry as a diagnostic technique.The results indicate the participation of electrochemically produced anthraquinone in the Michael addition reaction with acetylacetone and benzoylacetone to form the corresponding new anthraquinone derivatives.On the basis of the EC mechanism,the observed homogeneous rate constants（kobs）of the reaction of anthraquinone with acetylacetone and benzoylacetone were estimated by comparing the experimental cyclic voltammograms with the digitally simulated results.
文摘The conditions of color reaction of Aluminium(Ⅲ) with a new organic reagent 2,3,7 trihydroxy 9 [2,4 dihydroxy)phenylazo]phenylfluorone (ARPF) in the presence of cetyltrimethyammonium bromide(CTMAB) were studied. It was found that Aluminium(Ⅲ) reacts with ARPF and CTMAB in pH 6.5 HAc NaAc buffer solution to form a 1∶2 red complex with maximum absorption at 562 nm .The molar absorptivity is 1.10×10 5 L·mol -1 ·cm -1 . Beer's law is obeyed in the range of 0 5.5 μg Al(Ⅲ) per 25 mL. The method has been applied to determination of Aluminium in the limestone with satisfactory results.
基金supported by the Key Research and Development Program of Zhejiang Province(2025C01135)the National Natural Science Foundation of China(31770384,21605164)+2 种基金the Youth Academic Team Project of Minzu University of China(10301-02200301)the Huayi Technology Innovation Center for Research Resources(HTIC P01 RR2017001A)the Key Laboratory Construction Funds of State Ethnic Affairs Commission of China(10301-02200303)。
文摘Lipids are the basic components of cells that are involved in physiological processes such as energy transfer,signal transduction,cell growth,and apoptosis,thus playing important biological functions in organisms[1].Moreover,lipids are biomarkers for many diseases,including coronary heart disease,hepatitis C,and diabetes.A substantial number of studies have shown that obtaining accurate and comprehensive data on the spatial distribution of lipids is essential for investigating pathogenesis and identifying diagnostic biomarkers[2].
