[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading b...[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading bacterium were isolated from the sugar cane bagasse form different stages of natural fermentation,then,primary screening and secondary screening were performed.[Result] Starch-degrading strains s2g5-1 and s3g4-8 were screened,and they were identified as Bacillus amyloliquefaciens according to their morphological,physiological,biochemical and molecular characteristics.[Conclusion]The research provided theoretical basis for factory application of bagasse.展开更多
Memory deficits with aging are related to the neurodegeneration in the brain, including a reduction in arginine vasopressin(AVP) in the brain of patients with Alzheimer’s disease(AD). AVP(4-8), different from its pre...Memory deficits with aging are related to the neurodegeneration in the brain, including a reduction in arginine vasopressin(AVP) in the brain of patients with Alzheimer’s disease(AD). AVP(4-8), different from its precursor AVP, plays memory enhancement roles in the CNS without peripheral side-effects. However, it is not clear whether AVP(4-8) can improve cognitive behaviors and synaptic plasticity in the APP/PS1 mouse model of AD. Here, we investigated for the first time the neuroprotective effects of AVP(4-8) on memory behaviors and in vivo long-term potentiation(LTP) in APP/PS1-AD mice.The results showed that:(1) APP/PS1-AD mice had lower spontaneous alternation in the Y-maze than wild-type(WT) mice, and this was significantly reversed by AVP(4-8);(2) the prolonged escape latency of APP/PS1-AD mice in the Morris water maze was significantly decreased by AVP(4-8), and the decreased swimming time in target quadrant recovered significantly after AVP(4-8) treatment;(3) in vivo hippocampal LTP induced by high-frequency stimulation had a significant deficit in the AD mice, and this was partly rescued by AVP(4-8);(4) AVP(4-8)significantly up-regulated the expression levels of postsynaptic density 95(PSD95) and nerve growth factor(NGF)in the hippocampus of AD mice. These results reveal the beneficial effects of AVP(4-8) in APP/PS1-AD mice,showing that the intranasal administration of AVP(4-8)effectively improved the working memory and long-term spatial memory of APP/PS1-AD mice, which may be associated with the elevation of PSD95 and NGF levels in the brain and the maintenance of hippocampal synaptic plasticity.展开更多
The aluminophosphate molecular sieve VPI-5 has received much attention as a new structure containing one-dimensional circular pores circumscribed by 18 tetrahedral atoms. The AlPO;-8 is another new type of structure w...The aluminophosphate molecular sieve VPI-5 has received much attention as a new structure containing one-dimensional circular pores circumscribed by 18 tetrahedral atoms. The AlPO;-8 is another new type of structure which was found to contain 1 4-ring open pores. Recently, we have successfully synthesized the molecular sieves of VPI-5 and AlPO;-8, and found that the mixture of VPI-5 and AlPO;-8 was easily formed under similar conditions, and VPI-5 can be converted to AlPO;-8 by dehydration via heating. It is suggested that there exists relationship between the structures of VPI-5 and A1PO;-8. Therefore, the study on the transition and relationship between VPI-5 and A1PO;-8 is very important for designing and preparing new molecular sieves with new structures.展开更多
目的:慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)是老年人群常见且预后较差的慢性炎症性疾病,其进展常伴随免疫功能紊乱及气道重塑。本研究探讨血清肺泡表面活性蛋白-D(surfactant protein D,SP-D)、单核细胞趋化蛋...目的:慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)是老年人群常见且预后较差的慢性炎症性疾病,其进展常伴随免疫功能紊乱及气道重塑。本研究探讨血清肺泡表面活性蛋白-D(surfactant protein D,SP-D)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)及抗原分化簇(cluster of differentiation,CD)4受体(CD4^(+))/CD8^(+)水平预测老年COPD患者预后不良的价值,以期为早期干预和个体化管理提供参考。方法:回顾性分析2021年1月至2024年10月长沙市第三医院150例老年COPD患者的临床资料,参考《老年慢性阻塞性肺疾病管理指南》,采用COPD评估测试(COPD assessment test,CAT)联合临床终点事件作为预后分层标准,将患者分为预后良好组(36例)与预后不良组(114例)。比较2组一般资料,包括性别、年龄、体重指数(body mass index,BMI)、吸烟史、饮酒史、合并症。使用肺功能检测仪检测并计算患者第1秒用力呼气容积占预计值百分比(forced expiratory volume in 1 second percent predicted,FEV1%pred)、第1秒用力呼气容积与用力肺活量的比值(forced expiratory volume in 1 second/forced vital capacity,FEV1/FVC);以流式细胞仪检测CD4^(+)、CD8^(+)水平,并计算CD4^(+)/CD8^(+)比值;以酶联免疫吸附法测定血清MCP-1、SP-D水平;采用多因素Logistic回归分析COPD患者预后不良的危险因素。结果:2组性别、吸烟史、BMI、饮酒史、年龄、合并症比较,差异均无统计学意义(均P>0.05);预后不良组SP-D、CD8^(+)、MCP-1水平均高于预后良好组(均P<0.05);血清CD4^(+)、CD4^(+)/CD8^(+)和FEV1、FEV1/FVC水平均低于预后良好组(均P<0.05)。多因素Logistic回归分析结果显示FEV1%pred降低、FEV1/FVC降低、SP-D增高、CD4^(+)降低、CD8^(+)增高是老年COPD患者预后不良的危险因素(均P<0.05),CD4^(+)/CD8^(+)增高、MCP-1降低是老年COPD患者预后不良的保护因素(均P<0.05)。结论:FEV1%pred降低、FEV1/FVC降低、SP-D增高、CD4^(+)降低、CD8^(+)增高是老年COPD患者预后不良的危险因素,CD4^(+)/CD8^(+)增高、MCP-1降低是老年COPD患者预后不良的保护因素,需引起临床重视。展开更多
基金Supported by the Central Public-interest Scientific Institution Basal Research Fund(2008hzs1J021,2009hzs1J033)~~
文摘[Objective]The study aimed to screen the starch-degrading bacterium in bagasse and carry on the identification of strains s2g5-1 and s3g4-8.[Method]By using a variety of selective media,varieties of starch degrading bacterium were isolated from the sugar cane bagasse form different stages of natural fermentation,then,primary screening and secondary screening were performed.[Result] Starch-degrading strains s2g5-1 and s3g4-8 were screened,and they were identified as Bacillus amyloliquefaciens according to their morphological,physiological,biochemical and molecular characteristics.[Conclusion]The research provided theoretical basis for factory application of bagasse.
基金the National Natural Science Foundation of China(31471080)the Scientific Program for“Sanjin Scholars”of Shanxi Province,Shanxi“1331 Project”Key Subjects Construction(1331KSC)Science Foundation for Excellent Young Scholars of Shanxi Province,China(201801D211005)。
文摘Memory deficits with aging are related to the neurodegeneration in the brain, including a reduction in arginine vasopressin(AVP) in the brain of patients with Alzheimer’s disease(AD). AVP(4-8), different from its precursor AVP, plays memory enhancement roles in the CNS without peripheral side-effects. However, it is not clear whether AVP(4-8) can improve cognitive behaviors and synaptic plasticity in the APP/PS1 mouse model of AD. Here, we investigated for the first time the neuroprotective effects of AVP(4-8) on memory behaviors and in vivo long-term potentiation(LTP) in APP/PS1-AD mice.The results showed that:(1) APP/PS1-AD mice had lower spontaneous alternation in the Y-maze than wild-type(WT) mice, and this was significantly reversed by AVP(4-8);(2) the prolonged escape latency of APP/PS1-AD mice in the Morris water maze was significantly decreased by AVP(4-8), and the decreased swimming time in target quadrant recovered significantly after AVP(4-8) treatment;(3) in vivo hippocampal LTP induced by high-frequency stimulation had a significant deficit in the AD mice, and this was partly rescued by AVP(4-8);(4) AVP(4-8)significantly up-regulated the expression levels of postsynaptic density 95(PSD95) and nerve growth factor(NGF)in the hippocampus of AD mice. These results reveal the beneficial effects of AVP(4-8) in APP/PS1-AD mice,showing that the intranasal administration of AVP(4-8)effectively improved the working memory and long-term spatial memory of APP/PS1-AD mice, which may be associated with the elevation of PSD95 and NGF levels in the brain and the maintenance of hippocampal synaptic plasticity.
基金Supported by the National Natural Science Foundation of China
文摘The aluminophosphate molecular sieve VPI-5 has received much attention as a new structure containing one-dimensional circular pores circumscribed by 18 tetrahedral atoms. The AlPO;-8 is another new type of structure which was found to contain 1 4-ring open pores. Recently, we have successfully synthesized the molecular sieves of VPI-5 and AlPO;-8, and found that the mixture of VPI-5 and AlPO;-8 was easily formed under similar conditions, and VPI-5 can be converted to AlPO;-8 by dehydration via heating. It is suggested that there exists relationship between the structures of VPI-5 and A1PO;-8. Therefore, the study on the transition and relationship between VPI-5 and A1PO;-8 is very important for designing and preparing new molecular sieves with new structures.
文摘目的:慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)是老年人群常见且预后较差的慢性炎症性疾病,其进展常伴随免疫功能紊乱及气道重塑。本研究探讨血清肺泡表面活性蛋白-D(surfactant protein D,SP-D)、单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)及抗原分化簇(cluster of differentiation,CD)4受体(CD4^(+))/CD8^(+)水平预测老年COPD患者预后不良的价值,以期为早期干预和个体化管理提供参考。方法:回顾性分析2021年1月至2024年10月长沙市第三医院150例老年COPD患者的临床资料,参考《老年慢性阻塞性肺疾病管理指南》,采用COPD评估测试(COPD assessment test,CAT)联合临床终点事件作为预后分层标准,将患者分为预后良好组(36例)与预后不良组(114例)。比较2组一般资料,包括性别、年龄、体重指数(body mass index,BMI)、吸烟史、饮酒史、合并症。使用肺功能检测仪检测并计算患者第1秒用力呼气容积占预计值百分比(forced expiratory volume in 1 second percent predicted,FEV1%pred)、第1秒用力呼气容积与用力肺活量的比值(forced expiratory volume in 1 second/forced vital capacity,FEV1/FVC);以流式细胞仪检测CD4^(+)、CD8^(+)水平,并计算CD4^(+)/CD8^(+)比值;以酶联免疫吸附法测定血清MCP-1、SP-D水平;采用多因素Logistic回归分析COPD患者预后不良的危险因素。结果:2组性别、吸烟史、BMI、饮酒史、年龄、合并症比较,差异均无统计学意义(均P>0.05);预后不良组SP-D、CD8^(+)、MCP-1水平均高于预后良好组(均P<0.05);血清CD4^(+)、CD4^(+)/CD8^(+)和FEV1、FEV1/FVC水平均低于预后良好组(均P<0.05)。多因素Logistic回归分析结果显示FEV1%pred降低、FEV1/FVC降低、SP-D增高、CD4^(+)降低、CD8^(+)增高是老年COPD患者预后不良的危险因素(均P<0.05),CD4^(+)/CD8^(+)增高、MCP-1降低是老年COPD患者预后不良的保护因素(均P<0.05)。结论:FEV1%pred降低、FEV1/FVC降低、SP-D增高、CD4^(+)降低、CD8^(+)增高是老年COPD患者预后不良的危险因素,CD4^(+)/CD8^(+)增高、MCP-1降低是老年COPD患者预后不良的保护因素,需引起临床重视。
