目的研究外周血CD4^(+)CD28^(null)T细胞、肿瘤坏死因子受体超家族成员4(OX40)、4-1BB变化与糖尿病患者动脉粥样硬化(AS)进展的关联性。方法选取2型糖尿病(T2DM)患者120例,根据是否合并AS分为DM-AS组和DM组,比较两组外周血CD4^(+)CD28^(...目的研究外周血CD4^(+)CD28^(null)T细胞、肿瘤坏死因子受体超家族成员4(OX40)、4-1BB变化与糖尿病患者动脉粥样硬化(AS)进展的关联性。方法选取2型糖尿病(T2DM)患者120例,根据是否合并AS分为DM-AS组和DM组,比较两组外周血CD4^(+)CD28^(null)T细胞亚群比率及OX40、4-1BB阳性比率变化,并根据颈动脉狭窄程度分为轻度AS组(狭窄≤50%)、中度AS组(狭窄51%~69%)及重度AS组(狭窄≥70%),分析CD4^(+)CD28^(null)T细胞、OX40、4-1BB变化与AS进展的关联性。结果120例患者中,DM-AS组有76例,占63.33%;与DM组相比,DM-AS组的DM病程、收缩压、餐后2 h血糖(2 h PG)、糖化血红蛋白、载脂蛋白B、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平及CD4^(+)CD28^(null)T细胞亚群比率、OX40阳性比率、4-1BB阳性比率较高,载脂蛋白A1水平较低,差异均有统计学意义(t分别=2.57、2.49、2.78、2.19、3.12、2.94、4.31、5.97、16.91、11.47、-2.52,P均<0.05);CD4^(+)CD28^(null)T细胞、OX40、4-1BB联合诊断DM-AS的曲线下面积(AUC)为0.96。DM-AS组76例中,其中轻度AS组37例、中度AS组29例、重度AS组10例。AS程度与CD4^(+)CD28^(null)T细胞亚群比率及OX40、4-1BB阳性比率均呈正相关(r分别=0.24、0.48、0.38,P均<0.05)。结论DM-AS患者的外周血CD4^(+)CD28^(null)T细胞亚群比率及OX40、4-1BB阳性比率均明显升高,对DM-AS具有诊断价值,与AS进展呈正相关。展开更多
Pseudomonas fluorescens is a widespread spoilage bacterium in food,and its spoilage characteristics and biofilm formation are regulated by N-acyl-homoserine lactone(AHLs)quorum sensing(QS)system.Quorum quenching(QQ)is...Pseudomonas fluorescens is a widespread spoilage bacterium in food,and its spoilage characteristics and biofilm formation are regulated by N-acyl-homoserine lactone(AHLs)quorum sensing(QS)system.Quorum quenching(QQ)is considered as an effective strategy to control the spoilage bacteria.Therefore,this study revealed a new QQ bacteria Lactiplantibacillus plantarumYP4-1-2.Notably,the crude cell extract(CCE)from L.plantarum YP4-1-2 showed strongly QQ activity against the AHLs of P.fluorescens.The degradation rate of AHLs(N-octanoyl-L-homoserine lactone)reached 25.90%,78.57%and 100%at theCCE protein concentrations of 57,86 and 114 g/mL,respectively.In addition,the CCE could drastically reduce the formation of biofilm,bacterial motility(swimming and swarming),and the release of extracellular protease and biogenic amines of P.fluorescens.Real-time polymerase chain reaction(PCR)results revealed that the CCE downregulated QS-related genes(rhlI,rhlR,aprX,algA,orm,flgA,and ldcA).Finally,using whole-genome sequencing analysis,the active substance in the CCE was identified to be penicillin V acylase(PVA)and named LpPVA.Meanwhile,homologous modeling and molecular docking analysis showed that the binding effect of LpPVA on long-chain AHLs was better than that on short-chain AHLs.This study demonstrated that L.plantarum YP4-1-2 could be considered as a promising QS inhibitor and antibiofilm agent against foodborne spoilage bacteria.展开更多
目的探讨共刺激分子4-1BB/4-1BBL在过敏性紫癜性肾炎(HSPN)患儿外周血单个核细胞(PBMC)中的表达及其临床意义。方法选取初发过敏性紫癜无肾炎(HSP组)、初发过敏性紫癜性肾炎(HSPN组)患儿各20例(其中15例进行了肾脏病理检查),20例正常儿...目的探讨共刺激分子4-1BB/4-1BBL在过敏性紫癜性肾炎(HSPN)患儿外周血单个核细胞(PBMC)中的表达及其临床意义。方法选取初发过敏性紫癜无肾炎(HSP组)、初发过敏性紫癜性肾炎(HSPN组)患儿各20例(其中15例进行了肾脏病理检查),20例正常儿童为对照组,分离培养PBMC。应用逆转录-聚合酶链式反应(RT-PCR)检测并比较三组PBMC中4-1BB m RNA和4-1BBL m RNA的变化,分析4-1BB/4-1BBL m RNA变化与肾脏病理的关系。结果HSPN、HSP患儿PBMC中4-1 BB m RNA、4-1 BBL m RNA的表达均高于对照组,差异有统计学意义(P均<0.01)。HSPN患儿的病理积分与4-1BB/4-1BBL m RNA表达呈正相关关系(r=0.570、0.515,P均<0.05)。PBMC中,加入植物血凝素后,HSPN组、HSP组和对照组4-1 BB/4-1 BBL m RNA的表达均高于空白对照组,差异有统计学意义(P均<0.01),其中HSP、HSPN组增高幅度较对照组明显,HSPN组增高幅度较HSP组更为显著,差异有统计学意义(P均<0.01);加入地塞米松后,HSPN组、HSP组4-1 BB/4-1 BBL m RNA的表达均较空白对照组减少,差异有统计学意义(P均<0.01)。结论 HSPN患儿PBMC中共刺激分子4-1BB/4-1BBL m RNA的表达显著增高,植物血凝素能诱导其高表达,而地塞米松能抑制其表达,提示4-1BB/4-1BBL可能参与HSPN的发病。展开更多
文摘目的研究外周血CD4^(+)CD28^(null)T细胞、肿瘤坏死因子受体超家族成员4(OX40)、4-1BB变化与糖尿病患者动脉粥样硬化(AS)进展的关联性。方法选取2型糖尿病(T2DM)患者120例,根据是否合并AS分为DM-AS组和DM组,比较两组外周血CD4^(+)CD28^(null)T细胞亚群比率及OX40、4-1BB阳性比率变化,并根据颈动脉狭窄程度分为轻度AS组(狭窄≤50%)、中度AS组(狭窄51%~69%)及重度AS组(狭窄≥70%),分析CD4^(+)CD28^(null)T细胞、OX40、4-1BB变化与AS进展的关联性。结果120例患者中,DM-AS组有76例,占63.33%;与DM组相比,DM-AS组的DM病程、收缩压、餐后2 h血糖(2 h PG)、糖化血红蛋白、载脂蛋白B、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)水平及CD4^(+)CD28^(null)T细胞亚群比率、OX40阳性比率、4-1BB阳性比率较高,载脂蛋白A1水平较低,差异均有统计学意义(t分别=2.57、2.49、2.78、2.19、3.12、2.94、4.31、5.97、16.91、11.47、-2.52,P均<0.05);CD4^(+)CD28^(null)T细胞、OX40、4-1BB联合诊断DM-AS的曲线下面积(AUC)为0.96。DM-AS组76例中,其中轻度AS组37例、中度AS组29例、重度AS组10例。AS程度与CD4^(+)CD28^(null)T细胞亚群比率及OX40、4-1BB阳性比率均呈正相关(r分别=0.24、0.48、0.38,P均<0.05)。结论DM-AS患者的外周血CD4^(+)CD28^(null)T细胞亚群比率及OX40、4-1BB阳性比率均明显升高,对DM-AS具有诊断价值,与AS进展呈正相关。
基金supported by the Open Foundation of Oceanology Research Institute of Bohai University(BDHYYJY2020002).
文摘Pseudomonas fluorescens is a widespread spoilage bacterium in food,and its spoilage characteristics and biofilm formation are regulated by N-acyl-homoserine lactone(AHLs)quorum sensing(QS)system.Quorum quenching(QQ)is considered as an effective strategy to control the spoilage bacteria.Therefore,this study revealed a new QQ bacteria Lactiplantibacillus plantarumYP4-1-2.Notably,the crude cell extract(CCE)from L.plantarum YP4-1-2 showed strongly QQ activity against the AHLs of P.fluorescens.The degradation rate of AHLs(N-octanoyl-L-homoserine lactone)reached 25.90%,78.57%and 100%at theCCE protein concentrations of 57,86 and 114 g/mL,respectively.In addition,the CCE could drastically reduce the formation of biofilm,bacterial motility(swimming and swarming),and the release of extracellular protease and biogenic amines of P.fluorescens.Real-time polymerase chain reaction(PCR)results revealed that the CCE downregulated QS-related genes(rhlI,rhlR,aprX,algA,orm,flgA,and ldcA).Finally,using whole-genome sequencing analysis,the active substance in the CCE was identified to be penicillin V acylase(PVA)and named LpPVA.Meanwhile,homologous modeling and molecular docking analysis showed that the binding effect of LpPVA on long-chain AHLs was better than that on short-chain AHLs.This study demonstrated that L.plantarum YP4-1-2 could be considered as a promising QS inhibitor and antibiofilm agent against foodborne spoilage bacteria.
文摘目的探讨共刺激分子4-1BB/4-1BBL在过敏性紫癜性肾炎(HSPN)患儿外周血单个核细胞(PBMC)中的表达及其临床意义。方法选取初发过敏性紫癜无肾炎(HSP组)、初发过敏性紫癜性肾炎(HSPN组)患儿各20例(其中15例进行了肾脏病理检查),20例正常儿童为对照组,分离培养PBMC。应用逆转录-聚合酶链式反应(RT-PCR)检测并比较三组PBMC中4-1BB m RNA和4-1BBL m RNA的变化,分析4-1BB/4-1BBL m RNA变化与肾脏病理的关系。结果HSPN、HSP患儿PBMC中4-1 BB m RNA、4-1 BBL m RNA的表达均高于对照组,差异有统计学意义(P均<0.01)。HSPN患儿的病理积分与4-1BB/4-1BBL m RNA表达呈正相关关系(r=0.570、0.515,P均<0.05)。PBMC中,加入植物血凝素后,HSPN组、HSP组和对照组4-1 BB/4-1 BBL m RNA的表达均高于空白对照组,差异有统计学意义(P均<0.01),其中HSP、HSPN组增高幅度较对照组明显,HSPN组增高幅度较HSP组更为显著,差异有统计学意义(P均<0.01);加入地塞米松后,HSPN组、HSP组4-1 BB/4-1 BBL m RNA的表达均较空白对照组减少,差异有统计学意义(P均<0.01)。结论 HSPN患儿PBMC中共刺激分子4-1BB/4-1BBL m RNA的表达显著增高,植物血凝素能诱导其高表达,而地塞米松能抑制其表达,提示4-1BB/4-1BBL可能参与HSPN的发病。
