This study presents a visualized approach for tracking joint surface morphology.Three-dimensional laser scanning(3DLS)and 3D printing(3DP)techniques are adopted to record progressive failure during rock joint shearing...This study presents a visualized approach for tracking joint surface morphology.Three-dimensional laser scanning(3DLS)and 3D printing(3DP)techniques are adopted to record progressive failure during rock joint shearing.The 3DP resin is used to create transparent specimens to reproduce the surface morphology of a natural joint precisely.The freezing method is employed to enhance the mechanical properties of the 3DP specimens to reproduce the properties of hard rock more accurately.A video camera containing a charge-coupled device(CCD)camera is utilized to record the evolution of damaged area of joint surface during the direct shear test.The optimal shooting distance and shooting angle are recommended to be 800 mm and 40?,respectively.The images captured by the CCD camera are corrected to quantitatively describe the damaged area on the joint surface.Verification indicates that this method can accurately describe the total sheared areas at different shear stages.These findings may contribute to elucidating the shear behavior of rock joints.展开更多
Volume parameter is the basic content of a spatial body object morphology analysis.However,the challenge lies in the volume calculation of irregular objects.The point cloud slicing method proposed in this study effect...Volume parameter is the basic content of a spatial body object morphology analysis.However,the challenge lies in the volume calculation of irregular objects.The point cloud slicing method proposed in this study effectively works in calculating the volume of the point cloud of the spatial object obtained through three-dimensional laser scanning(3DLS).In this method,a uniformly spaced sequent slicing process is first conducted in a specific direction on the point cloud of the spatial object obtained through 3DLS.A series of discrete point cloud slices corresponding to the point cloud bodies are then obtained.Subsequently,the outline boundary polygon of the point cloud slicing is searched one by one in accordance with the slicing sequence and areas of the polygon.The point cloud slice is also calculated.Finally,the individual point cloud section volume is calculated through the slicing areas and the adjacent slicing gap.Thus,the total volume of the scanned spatial object can be calculated by summing up the individual volumes.According to the results and analysis of the calculated examples,the slice-based volume-calculating method for the point cloud of irregular objects obtained through 3DLS is correct,concise in process,reliable in results,efficient in calculation methods,and controllable on accuracy.This method comes as a good solution to the volume calculation of irregular objects.展开更多
目的构建可定量检测杀伤细胞免疫球蛋白样受体(KIR)转录水平的方法。方法筛选KIR3DS1和KIR3DL1保守区特异性引物,利用RT-PCR和熔解曲线法对引物特异性进行检验,建立可定量的标准内参,应用SYBR Green RT-PCR方法检测标本中KIR3DS1和KIR3...目的构建可定量检测杀伤细胞免疫球蛋白样受体(KIR)转录水平的方法。方法筛选KIR3DS1和KIR3DL1保守区特异性引物,利用RT-PCR和熔解曲线法对引物特异性进行检验,建立可定量的标准内参,应用SYBR Green RT-PCR方法检测标本中KIR3DS1和KIR3DL1的mRNA含量。结果通过电泳和实时定量PCR的熔解曲线明确了KIR3DS1和KIR3DL1引物,其特异性好,无杂带。应用TA克隆建立的KIR定量标准品线性关系好,可检测待测物的范围大。应用临床标本可检测出两组标本有显著差异,符合临床预期。结论本文建立的KIR mRNA定量检测方法,可以定量检测活化和抑制性KIR,可以进一步研究不同疾病进展的机制以及预测疾病的临床转归。展开更多
基金This experimental study was partially funded by the National Natural Science Foundation of China(Grant Nos.41572299and 41831290)the 3D-printed modeling work was supported by the Zhejiang Provincial Natural Science Foundation of China(Grant No.LY18D020003),which is gratefully acknowledged.
文摘This study presents a visualized approach for tracking joint surface morphology.Three-dimensional laser scanning(3DLS)and 3D printing(3DP)techniques are adopted to record progressive failure during rock joint shearing.The 3DP resin is used to create transparent specimens to reproduce the surface morphology of a natural joint precisely.The freezing method is employed to enhance the mechanical properties of the 3DP specimens to reproduce the properties of hard rock more accurately.A video camera containing a charge-coupled device(CCD)camera is utilized to record the evolution of damaged area of joint surface during the direct shear test.The optimal shooting distance and shooting angle are recommended to be 800 mm and 40?,respectively.The images captured by the CCD camera are corrected to quantitatively describe the damaged area on the joint surface.Verification indicates that this method can accurately describe the total sheared areas at different shear stages.These findings may contribute to elucidating the shear behavior of rock joints.
文摘Volume parameter is the basic content of a spatial body object morphology analysis.However,the challenge lies in the volume calculation of irregular objects.The point cloud slicing method proposed in this study effectively works in calculating the volume of the point cloud of the spatial object obtained through three-dimensional laser scanning(3DLS).In this method,a uniformly spaced sequent slicing process is first conducted in a specific direction on the point cloud of the spatial object obtained through 3DLS.A series of discrete point cloud slices corresponding to the point cloud bodies are then obtained.Subsequently,the outline boundary polygon of the point cloud slicing is searched one by one in accordance with the slicing sequence and areas of the polygon.The point cloud slice is also calculated.Finally,the individual point cloud section volume is calculated through the slicing areas and the adjacent slicing gap.Thus,the total volume of the scanned spatial object can be calculated by summing up the individual volumes.According to the results and analysis of the calculated examples,the slice-based volume-calculating method for the point cloud of irregular objects obtained through 3DLS is correct,concise in process,reliable in results,efficient in calculation methods,and controllable on accuracy.This method comes as a good solution to the volume calculation of irregular objects.
文摘目的构建可定量检测杀伤细胞免疫球蛋白样受体(KIR)转录水平的方法。方法筛选KIR3DS1和KIR3DL1保守区特异性引物,利用RT-PCR和熔解曲线法对引物特异性进行检验,建立可定量的标准内参,应用SYBR Green RT-PCR方法检测标本中KIR3DS1和KIR3DL1的mRNA含量。结果通过电泳和实时定量PCR的熔解曲线明确了KIR3DS1和KIR3DL1引物,其特异性好,无杂带。应用TA克隆建立的KIR定量标准品线性关系好,可检测待测物的范围大。应用临床标本可检测出两组标本有显著差异,符合临床预期。结论本文建立的KIR mRNA定量检测方法,可以定量检测活化和抑制性KIR,可以进一步研究不同疾病进展的机制以及预测疾病的临床转归。
