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Effects of impregnation sequence on the NH_(3)-SCR activity and hydrothermal stability of a Ce-Nb/SnO_(2) catalyst 被引量:1
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作者 Ying Zhu Wenpo Shan +3 位作者 Zhihua Lian Jingjing Liu Yan Zhang Hong He 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2024年第4期450-457,共8页
Hydrothermal stability is crucial for the practical application of deNO_(x)catalyst on diesel vehicles,for the selective catalytic reduction of NO_(x)with NH_(3)(NH_(3)-SCR).SnO_(2)-based materials possess superior hy... Hydrothermal stability is crucial for the practical application of deNO_(x)catalyst on diesel vehicles,for the selective catalytic reduction of NO_(x)with NH_(3)(NH_(3)-SCR).SnO_(2)-based materials possess superior hydrothermal stability,which is attractive for the development of NH_(3)-SCR catalyst.In this work,a series of Ce-Nb/SnO_(2)catalysts,with Ce and Nb loading on SnO_(2)support,were prepared by impregnation method.It was found that,the NH_(3)-SCR activities and hydrothermal stabilities of the Ce-Nb/SnO_(2)catalysts significantly varied with the impregnation sequences,and the Ce-Nb(f)/SnO_(2) catalyst that firstly impregnated Nb and then impregnated Ce exhibited the best performance.The characterization results revealed that CeNb(f)/SnO_(2)possessed appropriate acidity and redox capability.Furthermore,the strong synergistic effect between Nb and Sn species stabilized the structure and maintained the dispersion of acid sites.This study may provide a new understanding for the effect of impregnation sequence on activity and hydrothermal stability and a new environmental-friendly NH_(3)-SCR catalyst with potential applications for NO_(x)removal from diesel and hydrogenfueled engines. 展开更多
关键词 NO_(x)emission control NH_(3)-SCR Hydrothermal stability Ce-Nb/SnO_(2)catalyst Impregnation sequence
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Landscape of Sequence Variations in Homologous Copies of FAD2 and FAD3 in Rapeseed(Brassica napus L.)Germplasm with High/Low Linolenic Acid Trait
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作者 Haoxue Wu Xiaohan Zhang +5 位作者 Xiaoyu Chen Kang Li Aixia Xu Zhen Huang Jungang Dong Chengyu Yu 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第3期627-640,共14页
Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus breeding.B.napus is a tetraploid plant whose genome often har... Genetic manipulation(either restraint or enhancement)of the biosynthesis pathway ofα-linolenic acid(ALA)in seed oil is an important goal in Brassica napus breeding.B.napus is a tetraploid plant whose genome often har-bors four and six homologous copies,respectively,of the two fatty acid desaturases FAD2 and FAD3,which con-trol the last two steps of ALA biosynthesis during seed oil accumulation.In this study,we compared their promoters,coding sequences,and expression levels in three high-ALA inbred lines 2006L,R8Q10,and YH25005,a low-ALA line A28,a low-ALA/high-oleic-acid accession SW,and the wildtype ZS11.The expression levels of most FAD2 and FAD3 homologs in the three high-ALA accessions were higher than those in ZS11 and much higher than those in A28 and SW.The three high-ALA accessions shared similar sequences with the pro-moters and CDSs of BnFAD3.C4 and BnFAD3.A3.In A28 and SW,substitution of three amino acid residues in BnFAD2.A5 and BnFAD2.C5,an absence of BnFAD2.C1 locus,and a 549 bp long deletion on the BnFAD3.A3 promoter were detected.The profile of BnFAD2 mutation in the two low-ALA accessions A28 and SW is different from that reported in previous studies.The mutations in BnFAD3 in the high-ALA accessions are reported for thefirst time.In identifying the sites of these mutations,we provide detailed information to aid the design of mole-cular markers for accelerated breeding schemes. 展开更多
关键词 Brassica napus linolenic acid FAD2 FAD3 promoter coding sequences mutation
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3D-WATS序列与T_2 mapping测量正常成年男性膝关节软骨厚度的研究 被引量:8
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作者 孔延亮 强永乾 +1 位作者 董宝明 张蕾 《临床放射学杂志》 CSCD 北大核心 2013年第11期1626-1629,共4页
目的探讨1.5TMRI三维扰相脂肪抑制梯度回波(3D—WATS)序列与T2弛豫时间图(T2mapping)原始图像序列测量正常成年人膝关节关节软骨厚度差异性,评价T2mapping原始图像的临床应用价值。方法选取95名正常20~35岁成年男性膝关节,行矢... 目的探讨1.5TMRI三维扰相脂肪抑制梯度回波(3D—WATS)序列与T2弛豫时间图(T2mapping)原始图像序列测量正常成年人膝关节关节软骨厚度差异性,评价T2mapping原始图像的临床应用价值。方法选取95名正常20~35岁成年男性膝关节,行矢状位扫描,用3D—WATS和T2mapping原始图像,分别选取胫骨内外侧髁连线外1/4图像作为测量层面,每个膝关节测量平面选取3个测量点,测量结果用同源配对样本均数t检验比较测量值。结果正常成年男性膝关节软骨胫骨内外侧髁连线外侧1/4前后相同层面,相同测量点上3DWATS与T:mapping原始序列测量结果之间差异无统计学意义(t1=0.025,t2=1.868,P值均〉0.05)。在同层面胫骨软骨中点相同的测量点之间差异具有统计学意义(t=9.20,P〈0.05),3D—WATS较T2mapping序列测量软骨厚度大。结论正常成年人膝关节软骨厚度测量T2mapping原始图像可代替3D—WATS序列,且T2mapping序列原始灰阶图还可观察关节积液及半月板形态,T2mapping虽然测量胫骨软骨厚度不如3D—WATS序列,但仍可以观察软骨的连续是否完整。 展开更多
关键词 膝关节关节软骨3d-wats T2mapping测量方法
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二例SLC12A3基因复合杂合变异所致Gitelman综合征的遗传学分析
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作者 陈雪 田芯瑗 +4 位作者 郑雷 马盼盼 张钏 惠玲 周秉博 《国际生殖健康/计划生育杂志》 2025年第5期366-370,387,共6页
目的:总结2例Gitelman综合征患者的分子遗传学分析经验。方法:2例患者因反复发作的低血钾症就诊,临床高度怀疑Gitelman综合征。收集患者及其父母的外周静脉血样,提取基因组DNA,进行全外显子组测序(whole exome sequencing,WES)并分析结... 目的:总结2例Gitelman综合征患者的分子遗传学分析经验。方法:2例患者因反复发作的低血钾症就诊,临床高度怀疑Gitelman综合征。收集患者及其父母的外周静脉血样,提取基因组DNA,进行全外显子组测序(whole exome sequencing,WES)并分析结果。结果:经家系WES(trio-WES)数据分析,检测到2例患者的SLC12A3基因存在复合杂合变异,变异位点分别为c.965-1_976delinsACCGAAAATTTT和c.1964G>A、c.486_491delinsAC和c.2178+1G>T,父母均为表型正常的突变位点携带者,Sanger测序结果与trio-WES结果一致。结论:SLC12A3基因复合杂合致病性变异可能是这2例患者的遗传学病因,扩展了该基因的致病变异谱。对于可疑Gitelman综合征患者,应完善患者及其家系的基因检测以明确诊断,提供更全面的遗传咨询和更精确的再生育产前诊断。 展开更多
关键词 GITELMAN综合征 溶质载体家族12 成员3 全外显子组测序 突变 低钾血症
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MRI常规序列与3D-WATS序列对早期类风湿性关节炎手关节病变的诊断价值 被引量:6
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作者 张贵江 冯宪光 张莉 《临床和实验医学杂志》 2022年第1期94-96,共3页
目的探究磁共振成像(MRI)常规序列与三维扰相脂肪抑制梯度回波(3D-WATS)序列对早期类风湿性关节炎手关节病变的诊断价值。方法前瞻性选取2019年1月至2020年1月丹东市第一医院收治的80例疑似早期类风湿关节炎(RA)手关节病变患者作为研究... 目的探究磁共振成像(MRI)常规序列与三维扰相脂肪抑制梯度回波(3D-WATS)序列对早期类风湿性关节炎手关节病变的诊断价值。方法前瞻性选取2019年1月至2020年1月丹东市第一医院收治的80例疑似早期类风湿关节炎(RA)手关节病变患者作为研究对象。所有患者入院后分别行MRI常规序列及3D-WATS序列诊断,将诊断结果与病理结果对照,分析不同序列对早期RA手关节病变检出情况以及诊断的敏感性、准确性与特异性。结果病理诊断结果显示,80例患者中52例确诊为RA手关节病变阳性,28例为阴性;经3D-WATS序列诊断显示RA手关节病变阳性49例,阴性26例;MRI常规序列检出RA手关节病变阳性40例、阴性20例。3D-WATS序列诊断RA手关节病变的敏感性(94.23%)、特异性(92.86%)及准确率(93.75%)均显著高于MRI常规序列诊断(76.92%,71.43%,75.00%),差异均有统计学意义(P <0.05);3D-WATS序列对关节滑膜炎、骨侵蚀检出率(87.50%,66.25%)明显高于MRI常规序列(68.75%,50.00%),差异均有统计学意义(P <0.05)。结论 MRI 3D-WATS序列诊断早期类风湿性关节炎手关节病变敏感性、特异性及准确率高,能够有效检出关节滑膜炎、骨侵蚀等情况,优于MRI常规序列诊断,能够为临床治疗提供可靠依据,值得临床推广应用。 展开更多
关键词 类风湿性关节炎 手关节病变 MRI常规序列 3d-wats序列
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Matrix Representation of Recursive Sequences of Order 3 and Its Applications 被引量:2
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作者 Tianxiao HE Jeff H.-C.LIAO Peter J.-S.SHIUE 《Journal of Mathematical Research with Applications》 CSCD 2018年第3期221-235,共15页
Here presented is a matrix representation of recursive number sequences of order 3 defined by an = pa(n-1) + qa(n-2) + ra(n-3) with arbitrary initial conditions a0, a1 = 0, and a2 and their special cases of Pa... Here presented is a matrix representation of recursive number sequences of order 3 defined by an = pa(n-1) + qa(n-2) + ra(n-3) with arbitrary initial conditions a0, a1 = 0, and a2 and their special cases of Padovan number sequence and Perrin number sequence with initial conditions a0 = a1 = 0 and a2 = 1 and a0 = 3, a1 = 0, and a2 = 2, respectively. The matrix representation is used to construct many well known and new identities of recursive number sequences as well as Pavodan and Perrin sequences. 展开更多
关键词 recursive number sequence of order 3 matrix representation of recursive number sequences Padovan number sequence Perrin number sequence Tribonace polynomial sequence
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15株四川地区猪圆环病毒3型全基因序列的克隆与分析
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作者 肖璐 周泷 +11 位作者 吴学婧 于吉锋 谢晶 李兴玉 叶勇刚 魏勇 林毅 曹冶 潘梦 叶健强 毛从剑 康润敏 《中国动物传染病学报》 北大核心 2025年第1期171-180,共10页
为了解四川省地区猪圆环病毒3型(PCV3)流行毒株的分子及遗传变异规律,试验采用生物学软件,设计3对特异性引物对从四川地区部分养殖场猪群中收集的15份PCV3阳性样品进行分段扩增、全基因序列遗传变异分析。结果表明:PCR分段扩增分别得到... 为了解四川省地区猪圆环病毒3型(PCV3)流行毒株的分子及遗传变异规律,试验采用生物学软件,设计3对特异性引物对从四川地区部分养殖场猪群中收集的15份PCV3阳性样品进行分段扩增、全基因序列遗传变异分析。结果表明:PCR分段扩增分别得到大小为623、803和753 bp的条带,阳性克隆菌测序结果与GenBank中PCV3的同源性均大于98.6%;15株样品毒株的全基因核苷酸序列的同源性为98.1%~99.8%,推导氨基酸序列的同源性为95.8%~99.2%,由Cap基因的遗传进化分析发现从四川地区获取的15株PCV3毒株属于3a和3b两个亚群。说明四川地区流行的PCV3毒株至少存在3a和3b 2个亚群,该亚群的变异程度低、较为保守。 展开更多
关键词 猪圆环病毒3型(PCV3) 序列分析 遗传变异 流行病学
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A reduced computational load protein coding predictor using equivalent amino acid sequence of DNA string with period-3 based time and frequency domain analysis 被引量:1
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作者 Jayakishan K. Meher Gananath N. Dash +1 位作者 Pramod Kumar Meher Mukesh Kumar Raval 《American Journal of Molecular Biology》 2011年第2期79-86,共8页
Development of efficient gene prediction algorithms is one of the fundamental efforts in gene prediction study in the area of genomics. In genomic signal processing the basic step of the identification of protein codi... Development of efficient gene prediction algorithms is one of the fundamental efforts in gene prediction study in the area of genomics. In genomic signal processing the basic step of the identification of protein coding regions in DNA sequences is based on the period-3 property exhibited by nucleotides in exons. Several approaches based on signal processing tools and numerical representations have been applied to solve this problem, trying to achieve more accurate predictions. This paper presents a new indicator sequence based on amino acid sequence, called as aminoacid indicator sequence, derived from DNA string that uses the existing signal processing based time-domain and frequency domain methods to predict these regions within the billions long DNA sequence of eukaryotic cells which reduces the computational load by one-third. It is known that each triplet of bases, called as codon, instructs the cell machinery to synthesize an amino acid. The codon sequence therefore uniquely identifies an amino acid sequence which defines a protein. Thus the protein coding region is attributed by the codons in amino acid sequence. This property is used for detection of period-3 regions using amino acid sequence. Physico-chemical properties of amino acids are used for numerical representation. Various accuracy measures such as exonic peaks, discriminating factor, sensitivity, specificity, miss rate, wrong rate and approximate correlation are used to demonstrate the efficacy of the proposed predictor. The proposed method is validated on various organisms using the standard data-set HMR195, Burset and Guigo and KEGG. The simulation result shows that the proposed method is an effective approach for protein coding prediction. 展开更多
关键词 GENOMICS Bioinformatics CODON Coding region Amino Acid sequence Fourier Transform Antinotch Filter Periodicity-3 Indicator sequence
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磁共振3D-WATS在早期强直性脊柱炎骶髂关节软骨诊断中的应用价值 被引量:2
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作者 孟建超 《中国医药指南》 2020年第2期148-148,共1页
目的探讨在进行早期强直性脊柱炎骶髂关节软骨诊断期间磁共振3D-WATS的应用价值。方法选择我院2015年12月至2017年08月收治的84例早期强直性脊柱炎(AS)患者作为实验对象;于临床选择3.0T MRI展开骶髂关节平扫操作,最终对检查结果进行分... 目的探讨在进行早期强直性脊柱炎骶髂关节软骨诊断期间磁共振3D-WATS的应用价值。方法选择我院2015年12月至2017年08月收治的84例早期强直性脊柱炎(AS)患者作为实验对象;于临床选择3.0T MRI展开骶髂关节平扫操作,最终对检查结果进行分析。结果对于本次研究的84例患者,76例患者呈现出程度有所不同的骶髂关节软骨异常的情况。其中属于Ⅰ级患者32例,通过3D-WATS检查序列发现,其髂侧以及骶侧呈现出软骨厚薄不均的情况,呈现出形态略扭曲以及边缘欠光滑的情况,可疑点状软骨受到破坏;属于Ⅱ级患者28例,软骨斑点状呈现出不连续以及破坏现象,表现出局部缺损情况;属于Ⅲ级患者16例,能够发现软骨连续性破坏的情况,于斑点状呈现出软骨残存的情况。结论临床应用3D-WATS进行诊断,针对早期AS骶髂关节软骨病变可以清晰显示,针对其优势序列可以有效观察,从而证明3D-WATS应用可行性。 展开更多
关键词 磁共振3d-wats 早期强直性脊柱炎 骶髂关节软骨 应用价值
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Integration of Sequence Stratigraphic Analysis and 3D Geostatistical Modeling of Pliocene–Pleistocene Delta,F3 Block,Netherlands 被引量:1
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作者 Haris Ahmed KHAN Ali Asghar SHAHID +3 位作者 Muhammad Jahangir KHAN Taher ZOUAGHI Maria Dolores ALVAREZ Syed Danial Mehdi NAQVI 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2023年第1期256-268,共13页
This research is focused on the analysis of the sequence stratigraphic units of F3 Block,within a wave-dominated delta of Plio–Pleistocene age.Three wells of F3 block and a 3D seismic data,are utilized in this resear... This research is focused on the analysis of the sequence stratigraphic units of F3 Block,within a wave-dominated delta of Plio–Pleistocene age.Three wells of F3 block and a 3D seismic data,are utilized in this research.The conventional techniques of 3D seismic interpretation were utilized to mark the 11 surfaces on the seismic section.Integration of seismic sequence stratigraphic interpretation,using well logs,and subsequent 3D geostatistical modeling,using seismic data,aided to evaluate the shallow hydrocarbon traps.The resulting models were obtained using System Tract and Facies models,which were generated by using sequential stimulation method and their variograms made by spherical method,moreover,these models are validated via histograms.The CDF curve generated from upscaling of well logs using geometric method,shows a good relation with less percentage of errors(1 to 2 for Facies and 3 to 4 for System Tract models)between upscaled and raw data that complements the resulted models.These approaches help us to delineate the best possible reservoir,lateral extent of system tracts(LST and/or HST)in the respective surface,and distribution of sand and shale in the delta.The clinoform break points alteration observed on seismic sections,also validates the sequence stratigraphic interpretation.The GR log-based Facies model and sequence stratigraphy-based System Tract model of SU-04-2 showed the reservoir characteristics,presence of sand bodies and majorly LST,respectively,mainly adjacent to the main fault of the studied area.Moreover,on the seismic section,SU-04-2 exhibits the presence of gas pockets at the same location that also complements the generated Facies and System Tract models.The generated models can be utilized for any similar kind of study and for the further research in the F3 block reservoir characterization. 展开更多
关键词 sequence stratigraphy facies modeling system tract modeling F3 block North Sea
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Genetic Diversity of Chinese Soybean mosaic virus Strains and Their Relationships with Other Plant Potyviruses Based on P3 Gene Sequences 被引量:1
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作者 YANG Qing-hua LI Kai +1 位作者 ZHI Hai-jian GAI Jun-yi 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第10期2184-2195,共12页
Soybean mosaic virus (SMV), a member of the genus Potyvirus, is a major pathogen of soybean plants in China, and 16 SMV strains have been identified nationwide based on a former detailed SMV classification system. A... Soybean mosaic virus (SMV), a member of the genus Potyvirus, is a major pathogen of soybean plants in China, and 16 SMV strains have been identified nationwide based on a former detailed SMV classification system. As the P3 gene is thought to be involved in viral replication, systemic infection, pathogenicity, and overcoming resistance, knowledge of the P3 gene sequences of SMV and other potyviruses would be useful in efforts to know the genetic relationships among them and control the disease. P3 gene sequences were obtained from representative isolates of the above-mentioned 16 SMV strains and were compared with other SMV strains and 16 Potyvirus species from the National Center for Biotechnology GenBank database. The P3 genes from the 16 SMV isolates are composed of 1041 nucleotides, encoding 347 amino acids, and share 90.7-100% nucleotide (NT) sequence identities and 95.1-100% amino acid (AA) sequence identities. The P3 coding regions of the 16 SMV isolates share high identities (92.4-98.9% NT and 96.0-100% AA) with the reported Korean isolates, followed by the USA isolates (88.5-97.9% NT and 91.4-98.6% AA), and share low identities (80.5-85.2% NT and 82.1-84.7% AA) with the reported HZ 1 and P isolates from Pinellia ternata. The sequence identities of the P3 genes between SMV and the 16 potyviruses varied from 44.4 to 81.9% in the NT sequences and from 21.4 to 85.3% in the AA sequences, respectively. Among them, SMV was closely related to Watermelon mosaic virus (WMV), with 76.0-81.9% NT and 77.5-85.3% AA identities. In addition, the SMV isolates and potyvirus species were clustered into six distinct groups. All the SMV strains isolated from soybean were clustered in Group I, and the remaining species were clustered in other groups. A multiple sequence alignment analysis of the C-terminal regions indicated that the P3 genes within a species were highly conserved, whereas those among species were relatively variable. 展开更多
关键词 Soybean mosaic virus POTYVIRUS P3 gene homology analysis phylogenetic tree multiple sequence alignment
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Subtle traps prediction using sequence stratigraphy and 3D seismic technology: A case study from Qikou depression in Huanghua basin 被引量:1
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作者 MAO Ning-bo DAI Ta-gen PENG Sheng-lin 《Journal of Central South University of Technology》 2005年第z1期141-145,共5页
Forecasting subtle traps by sequence stratigraphy and 3D seismic data is a sensitive topic in hydrocarbon exploration. Research on subtle traps by geophysical data is the most popular and difficult. Based on the suffi... Forecasting subtle traps by sequence stratigraphy and 3D seismic data is a sensitive topic in hydrocarbon exploration. Research on subtle traps by geophysical data is the most popular and difficult. Based on the sufficiently drilling data, log data, core data and 3D seismic data, sediment sequence of Qikou depression, Huanghua basin was partitioned by using sequence stratigraphy theory. Each sediment sequence system mode was built. Sediment faces of subtle traps were pointed out. Dominating factors forming subtle traps were analyzed. Sandstone seismic rock physics and its response were studied in Tertiary System. Sandstone geophysical response and elastic modulus vary laws with pressure, temperature, porosity, depth were built. Experimental result and practice shows that it is possible using seismic information forecasting subtle traps. Integrated using geology, log, drilling data, special seismic processing technique, interpretation technique, high precision horizon calibration technique, 3D seismic visualizing interpretation, seismic coherence analysis, attribute analysis, logging-constrained inversion, time frequency analysis, subtle trapsobject is identified and interpreted. Finally, advantage object of subtle trap in this area was determined. Bottomland sand stratigraphic and lithologic reservoirs in Qinan slope zone have been founded by means of high resolution 3D seismic data field technique, high resolution 3D seismic data processing technique and seismic wave impendence inversion technique. 展开更多
关键词 Huanghua BASIN sequence STRATIGRAPHY subtle TRAP SEISMIC exploration 3D SEISMIC ultrasonic wave
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3-MCPD对雌、雄大鼠肾毒性影响的比较
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作者 黄江利 黄静怡 +8 位作者 苏伟谦 刘香梅 李玙萌 李培宁 何婷 刘思颖 全锦雯 黄宇锋 刘忠华 《中国兽医学报》 北大核心 2025年第2期322-329,340,共9页
为研究3-氯-1,2-丙二醇(3-monochloropropanel-1,2-diol, 3-MCPD)对雌、雄SD大鼠的肾毒性影响,试验通过灌胃不同剂量的3-MCPD建立肾毒性模型,将80只SD大鼠随机分为4组:对照组(0 mg/kg 3-MCPD)、低剂量组(15 mg/kg 3-MCPD)、中剂量组(30 ... 为研究3-氯-1,2-丙二醇(3-monochloropropanel-1,2-diol, 3-MCPD)对雌、雄SD大鼠的肾毒性影响,试验通过灌胃不同剂量的3-MCPD建立肾毒性模型,将80只SD大鼠随机分为4组:对照组(0 mg/kg 3-MCPD)、低剂量组(15 mg/kg 3-MCPD)、中剂量组(30 mg/kg 3-MCPD)、高剂量组(60 mg/kg 3-MCPD),雌、雄各1/2。每周记录大鼠体质量和摄食量,连续灌胃28 d后收集尿液、血液并采集肾脏组织,检测血液红细胞数(WBC)、白细胞数(RBC)、血红蛋白(HGB)、红细胞压积值(HCT)、肌酐(CREA)、血清尿素氮(BUN)及血磷(P)、血钙(Ca)指标;ELISA试剂盒测定尿液肾损伤分子(KIM-1)水平;组织病理学观察肾脏病理变化;转录组测序分析雌、雄大鼠差异基因。结果表明,3-MCPD不会显著影响雄性大鼠的生长,但高剂量会显著降低雌性大鼠的体质量及摄食量;高剂量组3-MCPD会造成雌、雄大鼠RBC、HGB、HCT水平显著下降,造成雌、雄大鼠KIM-1、P显著上升,Ca显著下降,但仅造成雌性大鼠CREA、BUN显著上升;组织病理学显示高剂量组的雄性大鼠肾脏仅出现轻度肾小管扩张、上皮细胞水肿、透明管型,而雌性大鼠出现大量肾小囊、透明管型、肾间质炎性细胞伴纤维化等严重病变;转录组测序显示高剂量组雌性大鼠差异基因有1 712个,高剂量组雄性大鼠差异基因有1 153个。KEGG富集分析表示高剂量组下雌、雄大鼠都会产生氧化应激和细胞凋亡,但3-MCPD可能还会通过抑制自噬、诱导铁死亡途径参与对雌性产生肾脏损伤的过程。以上结果表明高剂量3-MCPD会对雌性大鼠产生更显著的肾毒性作用。 展开更多
关键词 3-MCPD 大鼠 性别差异 肾毒性 转录组测序
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基质金属蛋白酶3参与小鼠矽肺纤维化的机制研究
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作者 王宇梦 罗薇 巢杰 《解放军医学杂志》 北大核心 2025年第4期458-466,共9页
目的探究基质金属蛋白酶3(MMP3)在二氧化硅(SiO_(2))诱导的小鼠矽肺模型中的表达情况及其参与矽肺纤维化的机制。方法6只C57B/6雄性小鼠随机分为对照组与矽肺组(n=3)。矽肺组小鼠经气管滴注SiO_(2)悬液0.2 g/kg构建矽肺模型,对照组经气... 目的探究基质金属蛋白酶3(MMP3)在二氧化硅(SiO_(2))诱导的小鼠矽肺模型中的表达情况及其参与矽肺纤维化的机制。方法6只C57B/6雄性小鼠随机分为对照组与矽肺组(n=3)。矽肺组小鼠经气管滴注SiO_(2)悬液0.2 g/kg构建矽肺模型,对照组经气管滴注等量生理盐水;采用5 ng/ml转化生长因子-β_(1)(TGF-β_(1))处理人成纤维细胞(HPF-a)和小鼠成纤维细胞(MLg)构建矽肺离体细胞模型。Masson染色和天狼腥红染色观察SiO_(2)对小鼠肺组织及其细胞外基质(ECM)的影响。对小鼠肺组织进行单细胞转录组测序,采用生物信息学方法分析矽肺组小鼠ECM相关细胞成分的变化及关键基因;空间转录组测序分析关键基因的表达与分布情况。Western blotting检测小鼠肺组织和成纤维细胞中波形蛋白和MMP3蛋白表达水平的改变;免疫荧光染色检测MMP3蛋白在小鼠肺组织ECM和TGF-β_(1)处理的HPF-a、MLg中的表达,以及在小鼠肺组织ECM中的蓄积情况。结果Masson染色和天狼腥红染色结果显示,矽肺组小鼠肺组织出现纤维化样改变且ECM胶原沉积明显。单细胞和空间转录组测序及相关生物信息学分析显示,矽肺组小鼠ECM成分改变与成纤维细胞相关,MMP3是其关键基因;矽肺组小鼠肺组织MMP3 mRNA表达水平升高,且主要分布于纤维化病灶部位。Western blotting检测结果显示,与对照组比较,矽肺组小鼠肺组织MMP3蛋白表达水平升高(P<0.05);TGF-β_(1)处理后,HPF-a中MMP3蛋白表达水平呈时间依赖性升高(P<0.05)。免疫荧光染色结果显示,与对照组比较,矽肺组小鼠肺组织ECM中MMP3蛋白表达水平升高(P<0.05);将TGF-β_(1)处理后的MLg移植到正常小鼠肺组织ECM后,其MMP3表达水平升高(P<0.05),MLg移植的小鼠肺组织ECM经脱细胞处理后,其MMP3表达水平同样升高(P<0.01);矽肺组小鼠肺组织中波形蛋白和MMP3在矽结节及其周围的共定位信号明显增强(P<0.01)。结论在小鼠矽肺模型中,TGF-β_(1)可导致成纤维细胞分泌的MMP3增多并潴留在肺ECM中,从而加重胶原沉积,促进肺纤维化。MMP3可能成为矽肺纤维化的潜在治疗靶点。 展开更多
关键词 基质金属蛋白酶3 细胞外基质 矽肺 单细胞转录组测序 空间转录组测序
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Type division and controlling factor analysis of 3rd-order sequences in marine carbonate rocks 被引量:1
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作者 Yunbo Zhang Zongju Zhao +4 位作者 Genhou Wang Zaixing Jiang Mingjian Wang Min Zheng Shiben Zhang 《Geoscience Frontiers》 SCIE CAS CSCD 2014年第2期289-298,共10页
Type division and controlling factor analysis of 3rd-order sequence are of practical significance to tec-tonic analysis, sedimentary environment identification, and other geological researches. Based on the comprehens... Type division and controlling factor analysis of 3rd-order sequence are of practical significance to tec-tonic analysis, sedimentary environment identification, and other geological researches. Based on the comprehensive analysis of carbon and oxygen isotope trends, paleobathymetry and spectral-frequency of representative well logs, 3rd-order sequences can be divided into 3 types: (a) global sea level (GSL) sequence mainly controlled by GSL change;(b) tectonic sequence mainly controlled by regional tectonic activity;and (c) composite sequence jointly controlled by GSL change and regional tectonic activity. This study aims to identify the controlling factors of 3rd-order sequences and to illustrate a new method for classification of 3rd-order sequences of the middle Permian strata in the Sichuan Basin, China. The middle Permian strata in the Sichuan Basin consist of 3 basin-contrastive 3rd-order sequences, i.e., PSQ1, PSQ2 and PSQ3. Of these, PSQ1 is a GSL sequence while PSQ2 and PSQ3 are composite sequences. The results suggest that the depositional environment was stable during the deposition of PSQ1, but was activated by tectonic activity during the deposition of the middle Permian Maokou Formation. 展开更多
关键词 Carbon isotope 3rd-Order sequence Milankovitch cycles Middle Permian strata Sichuan Basin
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2-酮丁酸通过成纤维细胞生长因子受体3调节心肌细胞活力
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作者 方克宝 付美娇 +4 位作者 徐方晶 张瑜 孙美琪 马占兵 何军 《宁夏医科大学学报》 2025年第3期223-232,共10页
目的观察2-酮丁酸(2-KB)对心肌细胞生长活性的影响,并探讨其分子机制。方法分别将大鼠心肌细胞H9C2及人心肌细胞AC16各自设对照组和实验组,以2-KB与实验组细胞共孵育16 h。通过CCK-8实验、结晶紫染色法和细胞划痕实验检测心肌细胞活力... 目的观察2-酮丁酸(2-KB)对心肌细胞生长活性的影响,并探讨其分子机制。方法分别将大鼠心肌细胞H9C2及人心肌细胞AC16各自设对照组和实验组,以2-KB与实验组细胞共孵育16 h。通过CCK-8实验、结晶紫染色法和细胞划痕实验检测心肌细胞活力。利用RNA测序筛选两种细胞中实验组与对照组共同的差异表达基因(differentially expressed genes,DEGs),通过CytoHubba筛选枢纽基因并经RT-qPCR验证,将验证后CytoHubba评分最高的基因进行基因干扰并检测干扰后心肌细胞活力的变化。结果CCK-8、结晶紫染色检测和划痕实验结果显示,实验组H9C2和AC16细胞活力均下降(P均<0.05)。RNA测序获得228个两种心肌细胞共有的DEGs,经CytoHubba筛选和RT-qPCR验证,获得4个关键枢纽基因,其中成纤维细胞生长因子受体3(fibroblast growth factor receptor 3,FGFR3)评分最高。FGFR3基因干扰实验结果显示,RNAi-FGFR3组心肌细胞活力较对照组和RNAi-vector组均降低(P均<0.05)。结论2-KB诱导心肌细胞活力降低、FGFR3表达上调,FGFR3表达上调可能是一种保护性机制。 展开更多
关键词 2-酮丁酸 心肌细胞活力 RNA测序 成纤维细胞生长因子受体3
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DAP-seq和RNA-seq揭示转录因子APETALA3-3调控铁皮石斛花型变化机理
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作者 宋雅蓉 朱洪凤 +3 位作者 敖叠 刘安祺 陆德银 和凤美 《热带作物学报》 北大核心 2025年第5期1052-1063,共12页
兰花唇瓣是特化的花瓣,因其多样性具有极大观赏价值和生物学价值,但其形成机理迄今仍未解决。APETALA3-3(AP3-3)是花器官身份基因,与兰花唇瓣形成有关,但其如何调控兰花唇瓣多样性形成机理目前未见报道。本研究以铁皮石斛野生型植株和... 兰花唇瓣是特化的花瓣,因其多样性具有极大观赏价值和生物学价值,但其形成机理迄今仍未解决。APETALA3-3(AP3-3)是花器官身份基因,与兰花唇瓣形成有关,但其如何调控兰花唇瓣多样性形成机理目前未见报道。本研究以铁皮石斛野生型植株和铁皮石斛DoAP3-3过表达植株的花器官为研究对象,通过DAP-seq和RNA-seq联合分析,从铁皮石斛转录因子DoAP3-3调控下游靶基因的角度探讨唇瓣多样性形成机制。结果表明:共鉴定出MADS6、NAC029、NAC054、WOX3、AS2、ERECTA、AG等7个关键候选靶基因,这些靶基因可能通过特异性调控或在DoAP3-3的作用下与其他基因互作调控花器官发育和花型发育。酵母单杂交实验验证表明转录因子DoAP3-3与MADS6之间存在相互作用,影响花型发育。该研究为初步解析花器官特征基因DoAP3-3与其靶基因之间的调控机制,探究兰科植物中高度特化和多样化的花形态形成机理奠定基础。 展开更多
关键词 APETALA3-3 铁皮石斛 DNA亲和纯化测序 DAP-seq 转录组测序 花型发育
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Cloning and Sequence Analysis of cDNA Encoding MRJP3 of Apis cerana cerana
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作者 SU Song-kun ZHNEG Huo-qing +2 位作者 CHEN Sheng-lu ZHONG Bo-xiong Stefan Albert 《Agricultural Sciences in China》 CAS CSCD 2005年第9期707-713,共7页
By screening the worker (Apis cerana cerana) heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 (AccMRJP3)... By screening the worker (Apis cerana cerana) heads cDNA library using a fragment of the mrjp3 gene of Apis cerana as probe, 120 positive clones were obtained. The clone containing A. cerana cerana MRJP3 (AccMRJP3) cDNA was selected. Based on the sequencing of the inserts of the positive clone, a sequence of AccMRJP3 cDNA which is 1 887 bp long including a poly (A) tail was obtained. The AccMRJP3 cDNA encompassed an open-reading frame (ORF) with 1 779 bp encoding 593 amino acids. The un-translated regions (UTR) of the 5′ end and 3′end are 46 bp and 160 bp in length, respectively. Similar to AmMRJP3 and AdMRJP3, the putative AccMRJP3 also has a repetitive region. The comparison of the repetitive region of AccMRJP3, AmMRJP3 and AdMRJP3 shows some differences between them. 展开更多
关键词 Apis cerana cerana MRJP3 Gene cloning sequence analysis
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星形胶质细胞通过Cx47调控外泌体介导的CHI3L1分泌并促进少突胶质前体细胞增殖
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作者 张笑妍 程楠楠 彭彦 《中南大学学报(医学版)》 北大核心 2025年第4期573-585,共13页
目的:神经退行性变性疾病与髓鞘缺失紧密相关,而少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)的增殖和分化是髓鞘修复的关键,但其调控机制尚未完全明确。本研究旨在探讨星形胶质细胞(astrocytes,ASTs)通过间隙连接蛋白47(co... 目的:神经退行性变性疾病与髓鞘缺失紧密相关,而少突胶质前体细胞(oligodendrocyte precursor cells,OPCs)的增殖和分化是髓鞘修复的关键,但其调控机制尚未完全明确。本研究旨在探讨星形胶质细胞(astrocytes,ASTs)通过间隙连接蛋白47(connexin 47,Cx47)调控外泌体介导的几丁质酶3样蛋白1(chitinase-3-like protein 1,CHI3L1)分泌及其对OPCs增殖的影响机制。方法:采用清洁的出生后第1天斯普拉格-道利(postnatal day 1 Sprague-Dawley,P1SD)乳鼠原代细胞,建立OPCs与ASTs上清液共培养组(A组)、ASTs与OPCs直接接触培养组(C组)及OPCs单独培养组(O组)。采用光学显微镜、5-乙炔基-2'-脱氧尿苷(5-ethynyl-2'-deoxyuridine,EdU)掺入及流式细胞术评估细胞状态和增殖能力。采用转录组测序(RNA sequencing,RNA-Seq)和生物信息学分析(bioinformatics analysis,BA)筛选3组中OPCs的差异表达基因(differentially expressed genes,DEGs)。采用蛋白质印迹(Western blotting,WB)和流式细胞术分析各培养组蛋白质表达和细胞周期。采用差速离心法分离、提纯外泌体,通过纳米颗粒追踪分析(nanoparticle tracking analysis,NTA)、透射电子显微镜分析观察OPCs分泌的外泌体数量和质量,采用WB验证外泌体中CHI3L1的表达水平。采用小干扰RNA(small interfering RNA,siRNA)技术敲除Cx47,评估敲除后OPCs增殖能力和外泌体分泌情况。构建包裹CHI3L1的小单层脂质体(single unilamellar vesicles,SUVs)作为人工外泌体模型,采用NTA和电子显微镜鉴定其结构和尺寸。在敲除Cx47后,补充人工外泌体并采用流式细胞术和EdU试验检测OPCs增殖情况。结果:在直接接触共培养条件下,相较于O组和A组,C组OPCs增殖最为显著(P<0.05)。RNA-Seq和WB分析结果显示,ASTs通过Cx47显著促进OPCs的增殖,并增强富含CHI3L1的外泌体的分泌。在采用siRNA技术抑制Cx47表达后,OPCs增殖活性以及外泌体释放量均显著降低(P<0.05);再次补充分离提纯的外泌体和外源性CHI3L1后,采用siRNA技术敲除Cx47对OPCs细胞增殖的抑制作用被削弱。结论:本研究揭示了ASTs通过Cx47调控OPCs增殖的新途径,ASTs在直接接触OPCs时通过Cx47增强富含CHI3L1的外泌体分泌,将细胞间接触信号转化为分泌信号,从而促进OPCs增殖。CHI3L1作为外泌体中的关键分子,在神经系统中的功能和髓鞘修复中的作用机制值得深入研究。 展开更多
关键词 少突胶质前体细胞 星形胶质细胞 间隙连接蛋白47 外泌体 几丁质酶3样蛋白1 5-乙炔基-2'-脱氧尿苷 转录组测序 差异表达基因 细胞增殖
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Content, Density, Illuviation Mode and Depth of CaCO<sub>3</sub>in Soils of Semiarid-Arid Qilian Mountains—An Altitude Sequence Study of the Hulugou Watershed
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作者 Ka Lin Decheng Li +4 位作者 Ganlin Zhang Yuguo Zhao Jinling Yang Feng Liu Xiaodong Song 《Agricultural Sciences》 2017年第6期479-491,共13页
The parental material of soils in the Qilian Mountains of northwest China is mainly aeolian loess containing CaCO3 which may remain in soils under the semiarid-arid climate. To disclose the CaCO3 characteristics chang... The parental material of soils in the Qilian Mountains of northwest China is mainly aeolian loess containing CaCO3 which may remain in soils under the semiarid-arid climate. To disclose the CaCO3 characteristics change with the altitude and the terrain attributes, we surveyed 18 soil profiles in an altitude sequence from 3076 m to 4510 m in the Hulugou Watershed in the Qilian Mountains, measured CaCO3 contents of all genetic horizon samples, analyzed the densities, illuviation modes and depths of CaCO3 in the profiles, extracted values of the terrain attributes of the profiles including altitude slope, aspect, plane curvature, profile curvature and terrain wetness index (TWI) from the 90 m resolution SRTM3 DEM data on ArcGIS 9.3 platform. We found that CaCO3 weighted content of the profiles ranged from 1.30 g·kg-1 to 93.09 g·kg-1, CaCO3 density from 0.05 kg/m2 to 75.69 kg/m2, CaCO3 illuviation depth from 12 cm to 54 cm. CaCO3 illuviation modes could be divided into three types, i.e., no illuviation mode in which the profile has only A horizon or CaCO3 content -1, middle illuviation mode in which CaCO3 accumulated in a middle horizon, and down illuviation mode in which CaCO3 content increases with the depth. CaCO3 weighted content, density and illuviation depth had significant correlation with certain terrain attributes. In general, the altitude sequence is an effective way to study CaCO3 characteristics in the alpine region, and the data of terrain attributes which can influence the precipitation and its redistribution in soil are potential in predicting soil CaCO3 characteristics in the alpine region. 展开更多
关键词 CaCO3 ALTITUDE sequence Terrain Attributes The Hulugou WATERSHED The QILIAN Mountains
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