This paper presents a simple technique to fabricate new electrofluidic devices for the three-dimensional(3D)manipulation of microorganisms by hybrid subtractive and additive femtosecond(fs)laser microfabrication(fs la...This paper presents a simple technique to fabricate new electrofluidic devices for the three-dimensional(3D)manipulation of microorganisms by hybrid subtractive and additive femtosecond(fs)laser microfabrication(fs laser-assisted wet etching of glass followed by water-assisted fs laser modification combined with electroless metal plating).The technique enables the formation of patterned metal electrodes in arbitrary regions in closed glass microfluidic channels,which can spatially and temporally control the direction of electric fields in 3D microfluidic environments.The fabricated electrofluidic devices were applied to nanoaquariums to demonstrate the 3D electro-orientation of Euglena gracilis(an elongated unicellular microorganism)in microfluidics with high controllability and reliability.In particular,swimming Euglena cells can be oriented along the z-direction(perpendicular to the device surface)using electrodes with square outlines formed at the top and bottom of the channel,which is quite useful for observing the motions of cells parallel to their swimming directions.Specifically,z-directional electric field control ensured efficient observation of manipulated cells on the front side(45 cells were captured in a minute in an imaging area of~160×120μm),resulting in a reduction of the average time required to capture the images of five Euglena cells swimming continuously along the z-direction by a factor of~43 compared with the case of no electric field.In addition,the combination of the electrofluidic devices and dynamic imaging enabled observation of the flagella of Euglena cells,revealing that the swimming direction of each Euglena cell under the electric field application was determined by the initial body angle.展开更多
We have proposed the novel design of 3D imaging pixel on-chip using a micro-conjugate mirror system,where the nonlinear microring resonator known as a Panda ring resonator is designed to form the micro-conjugate mirro...We have proposed the novel design of 3D imaging pixel on-chip using a micro-conjugate mirror system,where the nonlinear microring resonator known as a Panda ring resonator is designed to form the micro-conjugate mirror,which can be used to construct a three dimensional(3D)imaging system.Two micro-conjugate mirrors are vertically formed by stacking the two nonlinear Panda rings,which can be used to manipulate the 3D imaging perception,from which the human 3D vision can be replaced by a large area single eye device.In application,a thin film device of a single eye on-chip can be modeled and the artificial eye constructed.Simulation results obtained have shown that the single point(pixel)of an object can be interpreted to form the 3D image by the device whispering gallery modes(WGMs),from which the reference and the object beams are interfered and the 3D pixel formed.Finally,the holographic display information can be interpreted by the optic nerves and brain processing cells.The 3D image output signals can be confirmed theoretically by the symmetrical imaging signals.展开更多
提出了一种激光三维成像技术,该方法以推扫方式工作,采用数字微镜器件(Digital Mirror Device,DMD)来进行激光回波脉冲飞行时间(Time of Flight,TOF)的空间转换。由于目标上不同距离点回波脉冲的飞行时间不同,当脉冲到达时微透镜阵列将...提出了一种激光三维成像技术,该方法以推扫方式工作,采用数字微镜器件(Digital Mirror Device,DMD)来进行激光回波脉冲飞行时间(Time of Flight,TOF)的空间转换。由于目标上不同距离点回波脉冲的飞行时间不同,当脉冲到达时微透镜阵列将从一个状态转换到另一个状态,在接收端传感器焦平面上显示不同相对位置的条纹,利用条纹相对距离可以重建目标的剖面轮廓距离像。相比于其他三维成像技术,该技术具有成像速率高、探测视场角大、结构简单、体积小易于集成化等优点。展开更多
文摘This paper presents a simple technique to fabricate new electrofluidic devices for the three-dimensional(3D)manipulation of microorganisms by hybrid subtractive and additive femtosecond(fs)laser microfabrication(fs laser-assisted wet etching of glass followed by water-assisted fs laser modification combined with electroless metal plating).The technique enables the formation of patterned metal electrodes in arbitrary regions in closed glass microfluidic channels,which can spatially and temporally control the direction of electric fields in 3D microfluidic environments.The fabricated electrofluidic devices were applied to nanoaquariums to demonstrate the 3D electro-orientation of Euglena gracilis(an elongated unicellular microorganism)in microfluidics with high controllability and reliability.In particular,swimming Euglena cells can be oriented along the z-direction(perpendicular to the device surface)using electrodes with square outlines formed at the top and bottom of the channel,which is quite useful for observing the motions of cells parallel to their swimming directions.Specifically,z-directional electric field control ensured efficient observation of manipulated cells on the front side(45 cells were captured in a minute in an imaging area of~160×120μm),resulting in a reduction of the average time required to capture the images of five Euglena cells swimming continuously along the z-direction by a factor of~43 compared with the case of no electric field.In addition,the combination of the electrofluidic devices and dynamic imaging enabled observation of the flagella of Euglena cells,revealing that the swimming direction of each Euglena cell under the electric field application was determined by the initial body angle.
文摘We have proposed the novel design of 3D imaging pixel on-chip using a micro-conjugate mirror system,where the nonlinear microring resonator known as a Panda ring resonator is designed to form the micro-conjugate mirror,which can be used to construct a three dimensional(3D)imaging system.Two micro-conjugate mirrors are vertically formed by stacking the two nonlinear Panda rings,which can be used to manipulate the 3D imaging perception,from which the human 3D vision can be replaced by a large area single eye device.In application,a thin film device of a single eye on-chip can be modeled and the artificial eye constructed.Simulation results obtained have shown that the single point(pixel)of an object can be interpreted to form the 3D image by the device whispering gallery modes(WGMs),from which the reference and the object beams are interfered and the 3D pixel formed.Finally,the holographic display information can be interpreted by the optic nerves and brain processing cells.The 3D image output signals can be confirmed theoretically by the symmetrical imaging signals.
文摘提出了一种激光三维成像技术,该方法以推扫方式工作,采用数字微镜器件(Digital Mirror Device,DMD)来进行激光回波脉冲飞行时间(Time of Flight,TOF)的空间转换。由于目标上不同距离点回波脉冲的飞行时间不同,当脉冲到达时微透镜阵列将从一个状态转换到另一个状态,在接收端传感器焦平面上显示不同相对位置的条纹,利用条纹相对距离可以重建目标的剖面轮廓距离像。相比于其他三维成像技术,该技术具有成像速率高、探测视场角大、结构简单、体积小易于集成化等优点。
