目的:探究微小RNA(miR)-574-3p、序列相似家族3成员C(family with sequence similarity 3 member C,FAM3C)在食管癌组织中的表达,并分析二者与患者临床病理特征的关系。方法:选取2018年5月至2020年5月于本院行手术治疗的90例食管癌患者...目的:探究微小RNA(miR)-574-3p、序列相似家族3成员C(family with sequence similarity 3 member C,FAM3C)在食管癌组织中的表达,并分析二者与患者临床病理特征的关系。方法:选取2018年5月至2020年5月于本院行手术治疗的90例食管癌患者,收集术中留取的癌组织和癌旁组织标本。使用qRT-PCR法检测组织中miR-574-3p、FAM3C mRNA相对表达量;使用免疫组织化学法检测组织中FAM3C蛋白表达情况;采用Spearman等级相关分析食管癌组织中miR-574-3p、FAM3C的相关性;Kaplan-Meier法分析miR-574-3p、FAM3C与预后的关系。结果:食管癌患者癌组织中miR-574-3p低表达,FAM3C mRNA、FAM3C蛋白高表达(P<0.05)。食管癌组织中miR-574-3p、FAM3C蛋白表达水平具有负相关性(r=-0.420,P<0.05)。Pearson相关性分析显示,食管癌组织中miR-574-3p、FAM3C mRNA表达具有负相关性(r=-0.731,P<0.05)。miR-574-3p、FAM3C蛋白均与TNM分期、浸润程度、淋巴结转移有关(P<0.05)。miR-574-3p高表达组3年累积生存率为83.72%,显著高于miR-574-3p低表达组(53.19%);FAM3C高表达组3年累积生存率为56.86%,显著低于FAM3C低表达组(82.05%),差异均有统计学意义(log rankχ^(2)=10.175、7.290,P<0.05)。结论:食管癌组织中miR-574-3p表达水平较低,FAM3C表达水平较高,且二者具有负相关性,与临床病理特征中的TNM分期、浸润程度、淋巴结转移以及患者3年累积生存率有关。展开更多
AIM To obtain greater antigenicity of HCV NS3 protein. METHODS The HCV NS3 cDNA fragment was amplified by reverse transcription polymerase chain reaction from the sera of the HCV infected patients. The DNA sequence...AIM To obtain greater antigenicity of HCV NS3 protein. METHODS The HCV NS3 cDNA fragment was amplified by reverse transcription polymerase chain reaction from the sera of the HCV infected patients. The DNA sequence was determined by dideoxy mediated chain termination method using T7 polymerase. HCV NS3 protein was expressed in E. coli . RESULTS Sequence analysis indicated that the HCV isolate of this study belongs to HCV Ⅱ; SDS PAGE demonstrated an M r 23800 and an M r 22000 recombinant protein band which amount to 14% and 11% of the total bacterial proteins separately. Western blotting and ELISA showed NS3 protein possessed greater antigenicity. CONCLUSION Recombinant HCV NS3 protein was expressed successfully, which provided the basis for developing HCV diagnostic reagents.展开更多
In the search for a therapeutic schedule for spinal cord injury,it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process.However,ad hoc selection a...In the search for a therapeutic schedule for spinal cord injury,it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process.However,ad hoc selection and analysis of one or two genes cannot fully reveal the complex molecular biological mechanisms of spinal cord injury.The emergence of second-generation sequencing technology(RNA sequencing)has provided a better method.In this study,RNA sequencing technology was used to analyze differentially expressed genes at different time points after spinal cord injury in rat models established by contusion of the eighth thoracic segment.The numbers of genes that changed significantly were 944,1362 and 1421 at 1,4 and 7 days after spinal cord injury respectively.After gene ontology analysis and temporal expression analysis of the differentially expressed genes,C5ar1,Socs3 and CCL6 genes were then selected and identified by real-time polymerase chain reaction and western blot assay.The mRNA expression trends of C5ar1,Socs3 and CCL6 genes were consistent with the RNA sequencing results.Further verification and analysis of C5ar1 indicate that the level of protein expression of C5ar1 was consistent with its nucleic acid level after spinal cord injury.C5ar1 was mainly expressed in neurons and astrocytes.Finally,the gene Itgb2,which may be related to C5ar1,was found by Chilibot database and literature search.Immunofluorescence histochemical results showed that the expression of Itgb2 was highly consistent with that of C5ar1.Itgb2 was expressed in astrocytes.RNA sequencing technology can screen differentially expressed genes at different time points after spinal cord injury.Through analysis and verification,genes strongly associated with spinal cord injury can be screened.This can provide experimental data for further determining the molecular mechanism of spinal cord injury,and also provide possible targets for the treatment of spinal cord injury.This study was approved ethically by the Laboratory Animal Ethics Committee of Jiangsu Province,China(approval No.2018-0306-001)on March 6,2018.展开更多
Non-alcoholic fatty liver disease(NAFLD)is characterized by pathological lipid accumulation in hepatocytes.Cyanidin-3-rutinoside(C3R),a natural anthocyanin,attenuated lipid accumulation in a free fatty acid(FFA)-induc...Non-alcoholic fatty liver disease(NAFLD)is characterized by pathological lipid accumulation in hepatocytes.Cyanidin-3-rutinoside(C3R),a natural anthocyanin,attenuated lipid accumulation in a free fatty acid(FFA)-induced HepG2 steatosis model.It significantly reduced intracellular triglyceride(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)levels,suppressed lipid droplet formation,and alleviated oxidative stress by enhancing superoxide dismutase(SOD)/catalase(CAT)activities,as well as increasing glutathione(GSH)content,while lowering malondialdehyde(MDA)and reactive oxygen species(ROS).RNA sequencing analysis revealed that C3R reversed FFA-induced transcriptomic alterations.Mechanistically,C3R inhibited sterol regulatory element-binding protein 2(SREBP2)-mediated cholesterol synthesis,activated the proprotein convertase subtilisin/kexin type 9(PCSK9)/low-density lipoprotein receptor(LDLR)pathway to promote cholesterol clearance,and upregulated peroxisome proliferator-activated receptor gamma(PPARγ)-dependent cytochrome p450 family 7 subfamily a member 1(CYP7A1)expression to stimulate bile acid synthesis from cholesterol.C3R also enhanced autophagic flux,thereby facilitating the degradation of lipid droplets.Molecular docking confirmed the high-affinity binding of C3R to insulin-induced gene 1(INSIG1),PCSK9,and PPARγ,with hydroxyl groups forming specific hydrogen bonds at the active sites,supporting direct target engagement.These findings establish a multi-targeted mechanism for C3R in ameliorating hepatic steatosis,providing a molecular basis for anthocyanin-mediated regulation of lipid metabolism.展开更多
The modular multilevel matrix converter(M3C)is a potential frequency converter for low-frequency AC transmission.However,capacitor voltage control of high-voltage and largecapacity M3C is more difficult,especially for...The modular multilevel matrix converter(M3C)is a potential frequency converter for low-frequency AC transmission.However,capacitor voltage control of high-voltage and largecapacity M3C is more difficult,especially for voltage balancing between branches.To solve this problem,this paper defines sequence circulating components and theoretically analyzes the influence mechanism of different sequence circulating components on branch capacitor voltage.A fully decoupled branch energy balancing control method based on four groups of sequence circulating components is proposed.This method can control capacitor voltages of nine branches in horizontal,vertical and diagonal directions.Considering influences of both circulating current and voltage,a cross decoupled control is designed to improve control precision.Simulation results are taken from a low-frequency transmission system based on PSCAD/EMTDC,and effectiveness and precision of the proposed branch energy balancing control method are verified in the case of nonuniform parameters and an unbalanced power system.展开更多
Transcriptional regulatory regions are often located several thousand bases from the gene that they control.To function, the chromatin strand forms loops to juxtapose distal regions with the promoter. These long-range...Transcriptional regulatory regions are often located several thousand bases from the gene that they control.To function, the chromatin strand forms loops to juxtapose distal regions with the promoter. These long-rangechromatin interactions have profound influences on the regulation of gene expression and mapping these interactions iscurrently a subject of intensive investigation. Chromosome conformation capture (3C) technology and its derivativeshave been widely used to detect chromatin interactions and greatly contributed to understanding of the relationshipbetween genome organization and genome function. Here we review these 3C-based methods for the study of long-rangechromatin interactions and recent exciting findings obtained by using these technologies.展开更多
文摘AIM To obtain greater antigenicity of HCV NS3 protein. METHODS The HCV NS3 cDNA fragment was amplified by reverse transcription polymerase chain reaction from the sera of the HCV infected patients. The DNA sequence was determined by dideoxy mediated chain termination method using T7 polymerase. HCV NS3 protein was expressed in E. coli . RESULTS Sequence analysis indicated that the HCV isolate of this study belongs to HCV Ⅱ; SDS PAGE demonstrated an M r 23800 and an M r 22000 recombinant protein band which amount to 14% and 11% of the total bacterial proteins separately. Western blotting and ELISA showed NS3 protein possessed greater antigenicity. CONCLUSION Recombinant HCV NS3 protein was expressed successfully, which provided the basis for developing HCV diagnostic reagents.
基金supported by the National Natural Science Foundation of China,No.31570983(to XDW)the Priority Academic Program Development of Jiangsu Higher Education Institutes of China
文摘In the search for a therapeutic schedule for spinal cord injury,it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process.However,ad hoc selection and analysis of one or two genes cannot fully reveal the complex molecular biological mechanisms of spinal cord injury.The emergence of second-generation sequencing technology(RNA sequencing)has provided a better method.In this study,RNA sequencing technology was used to analyze differentially expressed genes at different time points after spinal cord injury in rat models established by contusion of the eighth thoracic segment.The numbers of genes that changed significantly were 944,1362 and 1421 at 1,4 and 7 days after spinal cord injury respectively.After gene ontology analysis and temporal expression analysis of the differentially expressed genes,C5ar1,Socs3 and CCL6 genes were then selected and identified by real-time polymerase chain reaction and western blot assay.The mRNA expression trends of C5ar1,Socs3 and CCL6 genes were consistent with the RNA sequencing results.Further verification and analysis of C5ar1 indicate that the level of protein expression of C5ar1 was consistent with its nucleic acid level after spinal cord injury.C5ar1 was mainly expressed in neurons and astrocytes.Finally,the gene Itgb2,which may be related to C5ar1,was found by Chilibot database and literature search.Immunofluorescence histochemical results showed that the expression of Itgb2 was highly consistent with that of C5ar1.Itgb2 was expressed in astrocytes.RNA sequencing technology can screen differentially expressed genes at different time points after spinal cord injury.Through analysis and verification,genes strongly associated with spinal cord injury can be screened.This can provide experimental data for further determining the molecular mechanism of spinal cord injury,and also provide possible targets for the treatment of spinal cord injury.This study was approved ethically by the Laboratory Animal Ethics Committee of Jiangsu Province,China(approval No.2018-0306-001)on March 6,2018.
基金supported by National Natural Science Foundation of China(No.31571825)Tianjin University Independent Innovation Foundation(2025XC-0001)2024 Graduate International Teaching System Construction Project(ENT24004).
文摘Non-alcoholic fatty liver disease(NAFLD)is characterized by pathological lipid accumulation in hepatocytes.Cyanidin-3-rutinoside(C3R),a natural anthocyanin,attenuated lipid accumulation in a free fatty acid(FFA)-induced HepG2 steatosis model.It significantly reduced intracellular triglyceride(TG),total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)levels,suppressed lipid droplet formation,and alleviated oxidative stress by enhancing superoxide dismutase(SOD)/catalase(CAT)activities,as well as increasing glutathione(GSH)content,while lowering malondialdehyde(MDA)and reactive oxygen species(ROS).RNA sequencing analysis revealed that C3R reversed FFA-induced transcriptomic alterations.Mechanistically,C3R inhibited sterol regulatory element-binding protein 2(SREBP2)-mediated cholesterol synthesis,activated the proprotein convertase subtilisin/kexin type 9(PCSK9)/low-density lipoprotein receptor(LDLR)pathway to promote cholesterol clearance,and upregulated peroxisome proliferator-activated receptor gamma(PPARγ)-dependent cytochrome p450 family 7 subfamily a member 1(CYP7A1)expression to stimulate bile acid synthesis from cholesterol.C3R also enhanced autophagic flux,thereby facilitating the degradation of lipid droplets.Molecular docking confirmed the high-affinity binding of C3R to insulin-induced gene 1(INSIG1),PCSK9,and PPARγ,with hydroxyl groups forming specific hydrogen bonds at the active sites,supporting direct target engagement.These findings establish a multi-targeted mechanism for C3R in ameliorating hepatic steatosis,providing a molecular basis for anthocyanin-mediated regulation of lipid metabolism.
基金supported by National Key R&D Program of China(No.2021YFB2401100).
文摘The modular multilevel matrix converter(M3C)is a potential frequency converter for low-frequency AC transmission.However,capacitor voltage control of high-voltage and largecapacity M3C is more difficult,especially for voltage balancing between branches.To solve this problem,this paper defines sequence circulating components and theoretically analyzes the influence mechanism of different sequence circulating components on branch capacitor voltage.A fully decoupled branch energy balancing control method based on four groups of sequence circulating components is proposed.This method can control capacitor voltages of nine branches in horizontal,vertical and diagonal directions.Considering influences of both circulating current and voltage,a cross decoupled control is designed to improve control precision.Simulation results are taken from a low-frequency transmission system based on PSCAD/EMTDC,and effectiveness and precision of the proposed branch energy balancing control method are verified in the case of nonuniform parameters and an unbalanced power system.
基金Research in the authors’laboratory is supported by the Intramural Research Program of the National Heart,Lung and Blood Institute,National Institutes of Health,USA.
文摘Transcriptional regulatory regions are often located several thousand bases from the gene that they control.To function, the chromatin strand forms loops to juxtapose distal regions with the promoter. These long-rangechromatin interactions have profound influences on the regulation of gene expression and mapping these interactions iscurrently a subject of intensive investigation. Chromosome conformation capture (3C) technology and its derivativeshave been widely used to detect chromatin interactions and greatly contributed to understanding of the relationshipbetween genome organization and genome function. Here we review these 3C-based methods for the study of long-rangechromatin interactions and recent exciting findings obtained by using these technologies.
