We analyze fluorescence due to oxidizing activity of DNA in neutrophils of peripheral blood in the large populations ~104 - 105 of cells. Fluorescence is registered by flow cytometry method. Spatial resolution is abou...We analyze fluorescence due to oxidizing activity of DNA in neutrophils of peripheral blood in the large populations ~104 - 105 of cells. Fluorescence is registered by flow cytometry method. Spatial resolution is about a few nanometers for varied complex three-dimensional (3D) DNA nanostructures of all non-coding and coding parts of DNA. It’s shown that oxidative activity of all 3D DNA in the full set of chromosomes inside cells is defined by new standards for complex networks of “exponentially small worlds”, with more dense packing than in the well known networks of “small worlds”. Analysis of various blood samples in vivo and during medical treatment shown that only two classes of Good and Bad Networks of DNA for a good and a bad health existed. This division is defined by any network to one from two classes of “n” or “s” shaped curves for typical deviations and from straight line in perfect networks of “exponentially small worlds”, as for two types of hysteresis curves at phase transitions or at switching of bistability. These deviations coincide with two types of positive and negative trends of changing fractal dimension by changing the scales of multi-scale networks of fluorescing DNA. These trends give the overall assessments of human immunity, including hidden and unidentified diseases, and as a sum of all kinds of health and illness of given person, from the point of view the inner life of neutrophils, living in different parts of human body in given time. Characteristics of deviations associated with type, level and complexity of illness in the dependence on展开更多
文摘We analyze fluorescence due to oxidizing activity of DNA in neutrophils of peripheral blood in the large populations ~104 - 105 of cells. Fluorescence is registered by flow cytometry method. Spatial resolution is about a few nanometers for varied complex three-dimensional (3D) DNA nanostructures of all non-coding and coding parts of DNA. It’s shown that oxidative activity of all 3D DNA in the full set of chromosomes inside cells is defined by new standards for complex networks of “exponentially small worlds”, with more dense packing than in the well known networks of “small worlds”. Analysis of various blood samples in vivo and during medical treatment shown that only two classes of Good and Bad Networks of DNA for a good and a bad health existed. This division is defined by any network to one from two classes of “n” or “s” shaped curves for typical deviations and from straight line in perfect networks of “exponentially small worlds”, as for two types of hysteresis curves at phase transitions or at switching of bistability. These deviations coincide with two types of positive and negative trends of changing fractal dimension by changing the scales of multi-scale networks of fluorescing DNA. These trends give the overall assessments of human immunity, including hidden and unidentified diseases, and as a sum of all kinds of health and illness of given person, from the point of view the inner life of neutrophils, living in different parts of human body in given time. Characteristics of deviations associated with type, level and complexity of illness in the dependence on
