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3D Spectrum Mapping and Reconstruction Under Multi-Radiation Source Scenarios
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作者 Wang Jie Lin Zhipeng +5 位作者 Zhu Qiuming Wu Qihui Lan Tianxu Zhao Yi Bai Yunpeng Zhong Weizhi 《China Communications》 2026年第2期20-34,共15页
Spectrum map construction,which is crucial in cognitive radio(CR)system,visualizes the invisible space of the electromagnetic spectrum for spectrum-resource management and allocation.Traditional reconstruction methods... Spectrum map construction,which is crucial in cognitive radio(CR)system,visualizes the invisible space of the electromagnetic spectrum for spectrum-resource management and allocation.Traditional reconstruction methods are generally for twodimensional(2D)spectrum map and driven by abundant sampling data.In this paper,we propose a data-model-knowledge-driven reconstruction scheme to construct the three-dimensional(3D)spectrum map under multi-radiation source scenarios.We firstly design a maximum and minimum path loss difference(MMPLD)clustering algorithm to detect the number of radiation sources in a 3D space.Then,we develop a joint location-power estimation method based on the heuristic population evolutionary optimization algorithm.Considering the variation of electromagnetic environment,we self-learn the path loss(PL)model based on the sampling data.Finally,the 3D spectrum is reconstructed according to the self-learned PL model and the extracted knowledge of radiation sources.Simulations show that the proposed 3D spectrum map reconstruction scheme not only has splendid adaptability to the environment,but also achieves high spectrum construction accuracy even when the sampling rate is very low. 展开更多
关键词 cognitive radio map reconstruction path loss model radiation source 3D spectrum map
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Path Planning for Substation UAV Inspection Based on 3D Point Cloud Mapping
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作者 Yanping Chen Zhengxin Zhan +3 位作者 Xiaohui Yan Le Zou Yucheng Zhong Hailei Wang 《Computers, Materials & Continua》 2026年第5期2138-2159,共22页
With the increasing complexity of substation inspection tasks,achieving efficient and safe path planning for Unmanned Aerial Vehicles in densely populated and structurally complex three-dimensional(3D)environments rem... With the increasing complexity of substation inspection tasks,achieving efficient and safe path planning for Unmanned Aerial Vehicles in densely populated and structurally complex three-dimensional(3D)environments remains a critical challenge.To address this problem,this paper proposes an improved path planning algorithm—Random Geometric Graph(RGG)-guided Rapidly-exploring Random Tree(R-RRT)—based on the classical Rapidly-exploring Random Tree(RRT)framework.First,a refined 3D occupancy grid map is constructed from Light Detection and Ranging point cloud data through ground filtering,noise removal,coordinate transformation,and obstacle inflation using spherical structuring elements.During the planning stage,a dynamic goal-biasing strategy is introduced to adaptively adjust the sampling direction,the sampling distribution is optimized using a pre-generated RGG,and collision detection is accelerated via a K-Dimensional Tree structure.After initial trajectory generation,redundant nodes are eliminated via greedy pruning,and a curvature-minimizing gradient-based optimizationmethod is applied to smooth the trajectory.Experimental results conducted in a simulated substation environment demonstrate that,compared with mainstream path planning algorithms,the proposed R-RRT achieves superior performance in terms of path length,planning time,and trajectory smoothness.Comprehensive analysis shows that the proposed method significantly enhances trajectory quality,planning efficiency,and operational safety,validating its applicability and advantages for high-precision 3D path planning in complex substation inspection scenarios. 展开更多
关键词 R-RRT algorithm unmanned aerial vehicles path planning random geometric graph 3D occupancy grid map substation inspection
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基于网络药理学及动物实验探讨补阳还五汤通过miR-30e-5p调控MAP3K5治疗低氧性肺动脉高压作用机制
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作者 王凯心 秦一冰 曲妮妮 《辽宁中医药大学学报》 2026年第3期15-24,共10页
目的基于网络药理学与动物实验探讨补阳还五汤对低氧性肺动脉高压(hypoxic pulmonary hypertension,HPH)大鼠血管重构的影响及其作用机制。方法(1)网络药理学实验。使用TCMSP、HERB、GeneCards、OMIM以及UniProt等公共数据库,筛选补阳... 目的基于网络药理学与动物实验探讨补阳还五汤对低氧性肺动脉高压(hypoxic pulmonary hypertension,HPH)大鼠血管重构的影响及其作用机制。方法(1)网络药理学实验。使用TCMSP、HERB、GeneCards、OMIM以及UniProt等公共数据库,筛选补阳还五汤和肺动脉高压(pulmonary arterial hypertension,PAH)的靶点基因并标准化;进行蛋白互作(protein-protein interaction,PPI)网络以及基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路富集分析;制作靶点交集韦恩图和“药物-成分-靶点-疾病”网络图;建立miRNAs-mRNAs网络互作图。(2)动物实验。将SD雄性大鼠50只随机分成5组,分别为空白组、模型组、中药低剂量组、中药中剂量组及中药高剂量组,每组10只,使用低氧暴露法建立HPH大鼠模型。4周后检测各组大鼠右心室压力;免疫组化测定肺小动脉细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达;ELISA法检测血清中P27^(kip1)的表达;Western blot法检测肺组织增殖、凋亡及通路相关蛋白的表达水平;实时定量逆转录聚合酶链反应(reverse transcription quantitative PCR,RT-qPCR)法检测各组大鼠肺组织中miR-30e-5p和丝裂原活化蛋白激酶激酶激酶5信号通路(mitogen-activated protein kinase kinase kinase 5 signaling pathway,MAP3K5)mRNA的表达。结果(1)网络药理学结果显示,补阳还五汤活性成分109个,相关靶点495个,疾病相关靶点7789个,交集靶点235个;GO功能富集分析共获得1038个条目,分别为生物过程(biological process,BP)774条、细胞组分(cellular component,CC)91条及分子功能(molecular function,MF)173条,KEGG富集结果175条;网络图分析出前5个主要疾病通路为肿瘤坏死因子(tumor necrosis factor,TNF)信号通路、白细胞介素-17(interleukin-17,IL-17)信号通路、晚期糖基化终末产物-其受体(advanced glycation end productsreceptor for AGE,AGE-RAGE)信号通路、MAP3K5和lipid and atherosclerosis信号通路;miRNAs-mRNAs网络互作图得出miR-30e-5p和MAP3K5关系密切。(2)与空白组相比,模型组右心室压力显著上升,肺小动脉结构改变;MAP3K5 mRNA及MAP3K5、B-细胞淋巴瘤2(B-cell lymphoma 2,Bcl-2)蛋白表达增加(P<0.01);miR-30e-5p mRNA及P27^(kip1)、细胞色素C(cytochrome C,Cyt C)、Bcl-2关联X蛋白(Bcl-2-associated X protein,Bax)、半胱天冬酶-3(Caspase-3)及半胱天冬酶-9(Caspase-9)蛋白表达降低(P<0.01);与模型组相比,中药各剂量组则显著降低了右心室压力,改善了肺小动脉结构,MAP3K5 mRNA及MAP3K5、Bcl-2蛋白表达减低(P<0.01),miR-30e-5p mRNA、P27^(kip1)、Cyt C、Bax、Caspase-3及Caspase-9蛋白表达升高(P<0.01或P<0.05)。结论该网络药理学和实验基本阐明,补阳还五汤可以通过miR-30e-5p调控MAP3K5,从而抑制增殖和促进凋亡,改善HPH大鼠肺血管重构,降低肺动脉压力。 展开更多
关键词 低氧性肺动脉高压 补阳还五汤 miR-30e-5p map3K5
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Zero-Shot Vision-Based Robust 3D Map Reconstruction and Obstacle Detection in Geometry-Deficient Room-Scale Environments
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作者 Taehoon Kim Sehun Lee Junho Ahn 《Computers, Materials & Continua》 2026年第2期602-631,共30页
As large,room-scale environments become increasingly common,their spatial complexity increases due to variable,unstructured elements.Consequently,demand for room-scale service robots is surging,yet most technologies r... As large,room-scale environments become increasingly common,their spatial complexity increases due to variable,unstructured elements.Consequently,demand for room-scale service robots is surging,yet most technologies remain corridor-centric,and autonomous navigation in expansive rooms becomes unstable even around static obstacles.Existing approaches face several structural limitations.These include the labor-intensive requirement for large-scale object annotation and continual retraining,as well as the vulnerability of vanishing point or linebased methods when geometric cues are insufficient.In addition,the high cost of LiDAR and 3D perception errors caused by limited wall cues and dense interior clutter further limit their effectiveness.To address these challenges,we propose a zero-shot vision-based algorithm for robust 3D map reconstruction in geometry-deficient room-scale environments.The algorithm operates in three layers:Layer 1 performs dimension-wise boundary detection;Layer 2 estimates vanishing points,refines the precise perspective space,and extracts a floor mask;and Layer 3 conducts 3D spatial mapping and obstacle recognition.The proposed method was experimentally validated across various geometric-deficient room-scale environments,including lobbies,seminar rooms,conference rooms,cafeterias,and museums—demonstrating its ability to reliably reconstruct 3D maps and accurately recognize obstacles.Experimental results show that the proposed algorithm achieved an F1 score of 0.959 in precision perspective space detection and 0.965 in floor mask extraction.For obstacle recognition and classification,it obtained F1 scores of 0.980 in obstacle absent areas,0.913 in solid obstacle environments,and 0.939 in skeleton-type sparse obstacle environments,confirming its high precision and reliability in geometric-deficient room-scale environments. 展开更多
关键词 Spatial AI zero-shot learning geometric deficiency 3D map reconstruction room-scale environment sparse obstacle precise classification
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3D Mapping技术驱动下展览空间设计创新路径研究
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作者 徐浩文 朱炜 《美与时代(创意)(上)》 2025年第7期132-135,共4页
数字媒体技术的迭代发展推动着文化传播范式的转型。当前展览领域普遍存在的静态展示模式面临双重挑战:物理空间的固定性与观众期待的动态叙事需求存在矛盾,单一维度的信息呈现方式难以满足公众对深度体验的追求。本研究聚焦3D Mapping... 数字媒体技术的迭代发展推动着文化传播范式的转型。当前展览领域普遍存在的静态展示模式面临双重挑战:物理空间的固定性与观众期待的动态叙事需求存在矛盾,单一维度的信息呈现方式难以满足公众对深度体验的追求。本研究聚焦3D Mapping技术在展览空间中的应用,通过具体案例分析其技术实现路径与艺术表达特征。3D Mapping技术有效解决了传统展览中物语分离、信息传递低效等问题,并显著提升观众的沉浸感与参与度。这也为数字时代展览空间的叙事重构提供创新路径。 展开更多
关键词 数字媒体 3D mapping 动态叙事 沉浸感
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MRI 3D-Vibe、T2 Mapping成像在腕关节TFCC损伤中的应用及软骨定量研究
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作者 杨泉 董胜超 +1 位作者 杨圣辉 王祺 《中国CT和MRI杂志》 2025年第11期187-189,195,共4页
目的探讨MRI三维容积插入式屏气检查及T2 mapping成像在腕关节三角纤维软骨复合体(TFCC)损伤应用及软骨定量分析中的应用价值。方法选取2023年7月至2024年6月于本院诊治的120例疑似腕关节TFCC损伤,以腕关节镜为金标准,经金标准确诊为腕... 目的探讨MRI三维容积插入式屏气检查及T2 mapping成像在腕关节三角纤维软骨复合体(TFCC)损伤应用及软骨定量分析中的应用价值。方法选取2023年7月至2024年6月于本院诊治的120例疑似腕关节TFCC损伤,以腕关节镜为金标准,经金标准确诊为腕关节TFCC损伤共78例,均在关节镜确诊前做有MRI 3D-Vibe、T2 mapping成像检查,观察并判断其TFCC损伤分型及T2值。采用四格表法计算MRI 3D-Vibe、T2 mapping成像诊断腕关节TFCC损伤的阳性、阴性预测值;绘制受试者工作特征(ROC)曲线分析MRI 3D-Vibe、T2 mapping成像诊断腕关节TFCC损伤的AUC、敏感度及特异度。结果120例疑似腕关节TFCC损伤行MRI 3D-Vibe检查,阳性和阴性预测值分别为81.58%、63.64%;行T2 mapping成像检查,阳性和阴性预测值分别为77.27%、50.00%;行两项联合检查,阳性和阴性预测值分别为82.95%、84.38%。两项联合诊断确诊率高于MRI 3D-Vibe、T2 mapping成像,但与MRI 3D-Vibe比较,差异无统计学意义(χ^(2)=2.526,P=0.112),与T2 mapping成像比较,差异具有统计学意义(χ^(2)=10.526,P<0.05)。阳性组的T2值(40.00±7.25)ms高于阴性组(χ^(2)=2.388,P<0.05);且ⅠB、C、D型的T2值高于ⅠA型(P<0.05)。ROC曲线分析显示,MRI 3D-Vibe、T2 mapping成像及两项联合诊断腕关节TFCC损伤的AUC值分别为(0.719、0.648、0.789,P<0.05);敏感度分别为79.49%、65.34%、93.59%;特异度均为66.67%、64.29%、64.29%。结论MRI 3D-Vibe联合T2 mapping成像在腕关节TFCC损伤诊断中具有较高效能,有利于降低误诊率。 展开更多
关键词 腕关节 MRI 3D-Vibe T2 mapping成像 TFCC
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Genetic Mapping of Grain Length-and Width-Related Genes in the Local Wheat Variety Guizi 1×Zhongyan 96-3 Hybrid Population Using Genome Sequencing
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作者 ShaoyanWu Jie Tian +6 位作者 YiyanWang Muhammad Arif ShuyaoWang JingWang Zhuoyao Yang Ruhong Xu Luhua Li 《Phyton-International Journal of Experimental Botany》 2025年第12期3913-3924,共12页
Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan... Wheat grain morphology,particularly grain length(GL)and width(GW),is a key determinant of yield.To improve the suboptimal grain dimensions of the local anthocyanin-rich variety Guizi 1(GZ1),we crossed it with Zhongyan 96-3(ZY96-3),an elite germplasm known for faster grain filling and superior grain size.A genotyping-by-sequencing(GBS)approach was applied to an F_(2)population of 110 individuals derived from GZ1×ZY96-3,resulting in the identification of 23,134 high-quality SNPs.Most of the SNPs associated with GL and GW were clustered on chromosomes 2B,3A,and 3B.QTL mapping for GL revealed two major loci,GL1 on chromosome 2B and GL2 on chromosome 3B,and eight candidate genes were identified within their corresponding intervals(2B:63.6–70.4 Mb;3B:631.5–633.3 Mb).These genes encode proteins potentially involved in grain size regulation,including a TOR2 regulation-associated protein,erect spike 2(EP2),fibroblast growth factor 6(FGF6),cellulose synthase-like(CSLD),RelA/pot homologue three family protein,and three GDSL esterase/lipase(GLIP)proteins.Additionally,we detected a QTL associated with GW on chromosome 3A and identified two candidate genes,TOR2 regulation and starch synthase within the 61.4–68.5 Mb interval.Overall,this study provides a strong theoretical and technical basis for wheat genetic improvement and offers valuable resources for precise QTL mapping and candidate gene discovery. 展开更多
关键词 WHEAT grain length grain width genotyping-by-sequencing(GBS) QTL mapping SNP markers candidate genes Guizi 1 Zhongyan 96-3
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MRI T_2 star mapping、T_1 images与3D DESS融合图在隐匿性膝关节软骨损伤中的应用 被引量:4
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作者 范伟雄 杨志企 +3 位作者 程凤燕 黄健 于昭 侯文忠 《临床医学工程》 2017年第4期437-439,共3页
目的探讨T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨损伤中的诊断价值。方法对26例关节软骨损伤患者行T_2 star mapping、T_1 images和3D DESS扫描,并将T_1 images、T_2 star mapping与3D DESS图像融合,评价患者股骨... 目的探讨T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨损伤中的诊断价值。方法对26例关节软骨损伤患者行T_2 star mapping、T_1 images和3D DESS扫描,并将T_1 images、T_2 star mapping与3D DESS图像融合,评价患者股骨、胫骨、髌骨关节软骨损伤程度并与关节镜结果对比,计算融合伪彩图诊断软骨损伤的特异性、敏感性及与关节镜诊断结果一致性。结果 T_1 images-3D DESS融合伪彩图诊断关节软骨损伤的敏感度、特异度及Kappa值分别为92.8%、93.0%、0.769,T_2 star mapping-3D DESS融合伪彩图诊断关节软骨损伤的敏感度、特异度及Kappa值分别为91.4%、94.2%、0.787。结论 T_2 star mapping、T_1 images与3D DESS融合伪彩图在关节软骨早期损伤评价上优于关节镜。 展开更多
关键词 膝关节 关节软骨 磁共振成像 T2 star mapping T1 IMAGES 3D DESS
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Comparative Physical Mapping of Bph3 with BAC-FISH in Oryza officinalis and O. sativa 被引量:3
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作者 鄢慧民 覃瑞 +2 位作者 金危危 何光存 宋运淳 《Acta Botanica Sinica》 CSCD 2002年第5期583-587,共5页
A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed ... A FISH procedure was adopted to physical mapping rice RFLP marker RZ69 and the BAC clone 38J9 screened by RZ69 which is linked to gene Bph3 in Oryza saliva L. and O. officinalis Well ex: Watt. The FISH results showed that both 38J9 and RZ69 were located in the middle of 4S in O. officinalis and the centromere area of 4S in O. sativa. In O. officinalis the percentage distances from the centromere to the hybridization sites were 20.00 +/- 5.40 and 22.12 +/- 3.44, the detection rates were 50.00% and 6.14%, but in O. sativa they were 0 and 0, 56.10% and 6.29% correspondingly. The results obtained from the BAC and RFLP clone were almost the same in the cultivated rice and O. officinalis. It was suggested that die marker RZ69 of the cultivated rice and its homologous sequence in O. officinalis were in the same BAC clone, the homologous sequence of Bph3 in O. officinalis should be located at the sites hybridized by both RZ69 and 38J9. Many signals on different chromosomes of O. officinalis were observed under no blocking with Cot-1 DNA, showing that the repetitive sequences were also homologous between O. sativa and O. officinalis. The identification of chromosome 4 of O. officinalis is based on comparative map with RG214 and BAC clone screened by RG214. The feasibility of comparative physical mapping performed between O. sativa and O. officinalis with rice BAC clones was discussed. 展开更多
关键词 Oryza officinalis BAC-FISH comparative physical mapping Bph3
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3D-WATS序列与T_2 mapping测量正常成年男性膝关节软骨厚度的研究 被引量:8
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作者 孔延亮 强永乾 +1 位作者 董宝明 张蕾 《临床放射学杂志》 CSCD 北大核心 2013年第11期1626-1629,共4页
目的探讨1.5TMRI三维扰相脂肪抑制梯度回波(3D—WATS)序列与T2弛豫时间图(T2mapping)原始图像序列测量正常成年人膝关节关节软骨厚度差异性,评价T2mapping原始图像的临床应用价值。方法选取95名正常20~35岁成年男性膝关节,行矢... 目的探讨1.5TMRI三维扰相脂肪抑制梯度回波(3D—WATS)序列与T2弛豫时间图(T2mapping)原始图像序列测量正常成年人膝关节关节软骨厚度差异性,评价T2mapping原始图像的临床应用价值。方法选取95名正常20~35岁成年男性膝关节,行矢状位扫描,用3D—WATS和T2mapping原始图像,分别选取胫骨内外侧髁连线外1/4图像作为测量层面,每个膝关节测量平面选取3个测量点,测量结果用同源配对样本均数t检验比较测量值。结果正常成年男性膝关节软骨胫骨内外侧髁连线外侧1/4前后相同层面,相同测量点上3DWATS与T:mapping原始序列测量结果之间差异无统计学意义(t1=0.025,t2=1.868,P值均〉0.05)。在同层面胫骨软骨中点相同的测量点之间差异具有统计学意义(t=9.20,P〈0.05),3D—WATS较T2mapping序列测量软骨厚度大。结论正常成年人膝关节软骨厚度测量T2mapping原始图像可代替3D—WATS序列,且T2mapping序列原始灰阶图还可观察关节积液及半月板形态,T2mapping虽然测量胫骨软骨厚度不如3D—WATS序列,但仍可以观察软骨的连续是否完整。 展开更多
关键词 膝关节关节软骨3D-WATS T2mapping测量方法
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牦牛MAP3K7基因克隆及其与皮下前体脂肪细胞分化关系分析
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作者 蓝湘一 何洋洋 +5 位作者 张明 黄慈 王海波 王会 柴志欣 钟金城 《华北农学报》 北大核心 2025年第3期225-233,共9页
MAP3K7是MAP3K家族中的一员,在脂肪分化、炎症和肿瘤发生过程中发挥着重要作用,但其与牦牛脂肪分化的关系未见报道,并且其基因序列未知,制约了相关功能研究。为了获得牦牛MAP3K7基因编码区(CDS)保守区域序列,明确其表达特征,了解其在牦... MAP3K7是MAP3K家族中的一员,在脂肪分化、炎症和肿瘤发生过程中发挥着重要作用,但其与牦牛脂肪分化的关系未见报道,并且其基因序列未知,制约了相关功能研究。为了获得牦牛MAP3K7基因编码区(CDS)保守区域序列,明确其表达特征,了解其在牦牛不同组织中的表达水平及其与牦牛皮下前体脂肪细胞分化的相关性,利用RT-PCR技术,克隆牦牛MAP3K7基因并利用生物信息学方法分析生物学特性;利用实时荧光定量PCR(qPCR)技术检测MAP3K7在牦牛心、肝、脾等10个组织中的表达情况,与不同分化阶段牦牛皮下脂肪细胞中MAP3K7基因和脂分化相关基因的表达情况,同时结合油红O染色,明确MAP3K7基因对牦牛皮下前体脂肪细胞分化的影响。结果表明:克隆所得牦牛MAP3K7基因CDS区长度为1740 bp,编码579个氨基酸,与野牦牛MAP3K7预测序列CDS区比对发现第967位A>G,导致第323位氨基酸Thr>Ala的突变。理化性质分析发现,该蛋白为整体带负电的亲水酸性蛋白,具有112个氨基酸磷酸化位点,其氨基酸组成中,占比最高的是丝氨酸;二级结构预测显示,其无规卷曲含量最高,可能与TAB3等蛋白质间存在相互作用关系。核苷酸同源性比对与进化树结果表明,牦牛MAP3K7在反刍动物间具有较高的保守性,与野牦牛同源性最高且进化关系最近。组织定量检测发现,MAP3K7在牦牛皮下脂肪和内脏脂肪中的表达量相对较低,在牦牛皮下前体脂肪细胞诱导分化过程中,MAP3K7表达量随着成熟脂肪细胞的增多先上调,至分化到一定程度后下调。综上,MAP3K7基因在牦牛皮下前体脂肪细胞分化前期高表达以促进分化,但当分化到一定程度时,表达下调抑制进一步分化。 展开更多
关键词 牦牛 map3K7 基因表达 皮下脂肪 脂肪分化
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视错觉在3D Mapping投影中的应用研究 被引量:5
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作者 赵昱东 席涛 《包装工程》 CAS 北大核心 2018年第14期65-69,共5页
目的旨在研究提升3D Mapping制作质量的方法。通过借鉴视错觉对于艺术、建筑、环境、服饰等领域的研究,分析3D Mapping的特点以及视错觉的影响,找出对于3D Mapping具有指导性理论及应用。方法在解析3D Mapping制作方法的同时,将3D Mapp... 目的旨在研究提升3D Mapping制作质量的方法。通过借鉴视错觉对于艺术、建筑、环境、服饰等领域的研究,分析3D Mapping的特点以及视错觉的影响,找出对于3D Mapping具有指导性理论及应用。方法在解析3D Mapping制作方法的同时,将3D Mapping中常见的视错觉进行分析,着重研究深度错觉、反透视原理以及边框错觉的视错觉原理进行深度分析。分析这3种视错觉在实际案例之中是如何具体应用,研究视错觉如何对3D Mapping产生影响。结论正确的利用深度错觉、反透视原理、边框错觉等视错觉可以对3D Mapping进行视觉深度的改变、良好的定位校正以及震撼的视觉效果。 展开更多
关键词 新媒体艺术 装置艺术 视错觉 3D mapping
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枸杞多糖通过MAP3K3通路降低PD-L1表达抗肺腺癌作用及机制
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作者 陈思柔 陈文雅 +4 位作者 陈静芹 黄秋茵 李龙娟 张韧 何彦丽 《中药新药与临床药理》 北大核心 2025年第9期1458-1470,共13页
目的探究枸杞多糖通过MAP3K3通路降低程序性死亡配体1(PD-L1)表达从而抗肺腺癌的作用及其机制。方法(1)体内实验:构建小鼠皮下肺腺癌移植瘤模型,观察枸杞多糖低、中、高(25、50、100 mg·kg^(-1))剂量用药对小鼠肿瘤生长及免疫器官... 目的探究枸杞多糖通过MAP3K3通路降低程序性死亡配体1(PD-L1)表达从而抗肺腺癌的作用及其机制。方法(1)体内实验:构建小鼠皮下肺腺癌移植瘤模型,观察枸杞多糖低、中、高(25、50、100 mg·kg^(-1))剂量用药对小鼠肿瘤生长及免疫器官的影响。(2)体外实验:枸杞多糖0.25、0.5、1 mg·mL^(-1)处理H1299、A549细胞48 h,CCK-8法及平板克隆形成法检测各组细胞增殖情况,Western Blot及RT-qPCR法检测各组PD-L1蛋白和mRNA表达水平;建立来自健康人外周血激活的T淋巴细胞(PBMC)与肺腺癌细胞共培养体系,观察枸杞多糖用药前后对PBMC免疫杀伤作用的影响;1 mg·mL^(-1)枸杞多糖干预H1299细胞后进行转录组学测序,筛选差异表达基因,并进行KEGG富集分析。以Western Blot和RT-qPCR法检测枸杞多糖用药后肺腺癌细胞MAP3K3蛋白和mRNA表达水平。(3)回复实验:构建MAP3K3过表达A549人肺腺癌细胞株(A549 MAP3K3OE),Western Blot法检测枸杞多糖用药后其PD-L1蛋白表达情况,并观察MAP3K3基因过表达前后以及枸杞多糖干预前后,PBMC对肺腺癌细胞免疫杀伤功能的影响。结果(1)与对照组比较,枸杞多糖用药组瘤质量明显下降(P<0.05,P<0.01,P<0.001);肿瘤组织病理学观察发现细胞密度降低,间质淋巴细胞浸润增多;枸杞多糖用药组免疫器官脾脏系数上升(P<0.05)。(2)枸杞多糖处理人肺腺癌细胞48 h后细胞增殖活力无明显变化(P>0.05);枸杞多糖用药后细胞PD-L1蛋白和mRNA表达水平均下降(P<0.05,P<0.01,P<0.001);枸杞多糖可增强激活的PBMC对肺腺癌细胞的免疫杀伤作用(P<0.05,P<0.01,P<0.001);转录组学测序差异基因主要富集在MAPK通路;枸杞多糖用药组细胞MAP3K3蛋白和mRNA表达水平下调(P<0.05,P<0.01,P<0.001)。(3)A549 MAP3K3 OE肺腺癌细胞中PD-L1高表达(P<0.01);共培养实验发现A549MAP3K3 OE细胞对激活的PBMC免疫杀伤作用具有一定的抵抗性(P<0.001),而枸杞多糖干预后,可一定程度恢复PBMC对A549 MAP3K3 OE细胞的杀伤敏感性(P<0.01,P<0.001)。结论枸杞多糖通过下调肺腺癌细胞MAP3K3基因及PD-L1表达,改善体外培养体系中激活PBMC的免疫杀伤功能,这可能是枸杞多糖抗肺腺癌的作用机制之一。 展开更多
关键词 枸杞多糖 map3K3通路 PD-L1 肺腺癌 免疫杀伤 小鼠 H1299细胞 A549细胞 HBE细胞 Lewis细胞
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利用CRISPR/Cas9基因编辑技术构建斑马鱼map3k15敲除品系
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作者 贾淳钰 沈延 +3 位作者 陈罡 郑可 秦岩 李雪梅 《基础医学与临床》 2025年第3期290-297,共8页
目的利用CRISPR/Cas9基因编辑技术构建斑马鱼map3k15敲除纯合品系,为进一步研究map3k15在肾脏疾病方面的作用提供动物模型。方法1)分析map3k15在斑马鱼中的表达模式;2)利用CRISPR/Cas9基因编辑技术构建斑马鱼map3k15敲除纯合品系;3)观察... 目的利用CRISPR/Cas9基因编辑技术构建斑马鱼map3k15敲除纯合品系,为进一步研究map3k15在肾脏疾病方面的作用提供动物模型。方法1)分析map3k15在斑马鱼中的表达模式;2)利用CRISPR/Cas9基因编辑技术构建斑马鱼map3k15敲除纯合品系;3)观察map3k15缺失对斑马鱼表型的影响。结果1)map3k15在斑马鱼前肾中表达,且MAP3K15/Map3k15蛋白在多物种间具有高度保守性;2)成功构建了斑马鱼map3k15-/-突变体,并保留+2 bp及+1 bp 2种突变品系;3)map3k15突变斑马鱼在胚胎发育过程中出现卵黄囊、心包及头部的水肿,且随发育时间延长,症状逐渐加重。结论成功构建了map3k15敲除的纯合斑马鱼品系,为未来研究map3k15在肾脏发育及疾病中的作用提供了重要的模型。 展开更多
关键词 map3k15 CRISPR/Cas9基因编辑技术 斑马鱼 肾脏发育
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3D Mineral Prospectivity Mapping Based on Deep Metallogenic Prediction Theory:A Case Study of the Lala Copper Mine,Sichuan,China 被引量:9
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作者 Keyan Xiao Jie Xiang +1 位作者 Mingjing Fan Yang Xu 《Journal of Earth Science》 SCIE CAS CSCD 2021年第2期348-357,共10页
With the decrease in surface and shallow ore deposits,mineral exploration has focused on deeply buried ore bodies,and large-scale metallogenic prediction presents new opportunities and challenges.This paper adopts the... With the decrease in surface and shallow ore deposits,mineral exploration has focused on deeply buried ore bodies,and large-scale metallogenic prediction presents new opportunities and challenges.This paper adopts the predictive thinking method in this era of big data combined with specific research on the special exploration and exploitation of deep-earth resources.Four basic theoretical models of large-scale deep mineralization prediction and evaluation are explored:mineral prediction geological model theory,multidisciplinary information correlation theory,mineral regional trend analysis theory,and mineral prediction geological differentiation theory.The main workflow of large-scale deep resource prediction in the digital and information age is summarized,including construction of ore prospecting models of metallogenic systems,multiscale 3 D geological modeling,and 3 D quantitative prediction of deep resources.Taking the Lala copper mine in Sichuan Province as an example,this paper carries out deep 3 D quantitative prediction of mineral resources and makes a positive contribution to the future prediction and evaluation of mineral resources. 展开更多
关键词 deep mine resources prospectivity mapping 3D modeling quantitative evaluation Lala copper mine
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Location estimation of autonomous driving robot and 3D tunnel mapping in underground mines using pattern matched LiDAR sequential images 被引量:7
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作者 Heonmoo Kim Yosoon Choi 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2021年第5期779-788,共10页
In this study,a machine vision-based pattern matching technique was applied to estimate the location of an autonomous driving robot and perform 3D tunnel mapping in an underground mine environment.The autonomous drivi... In this study,a machine vision-based pattern matching technique was applied to estimate the location of an autonomous driving robot and perform 3D tunnel mapping in an underground mine environment.The autonomous driving robot continuously detects the wall of the tunnel in the horizontal direction using the light detection and ranging(Li DAR)sensor and performs pattern matching by recognizing the shape of the tunnel wall.The proposed method was designed to measure the heading of the robot by fusion with the inertial measurement units sensor according to the pattern matching accuracy;it is combined with the encoder sensor to estimate the location of the robot.In addition,when the robot is driving,the vertical direction of the underground mine is scanned through the vertical Li DAR sensor and stacked to create a 3D map of the underground mine.The performance of the proposed method was superior to that of previous studies;the mean absolute error achieved was 0.08 m for the X-Y axes.A root mean square error of 0.05 m^(2)was achieved by comparing the tunnel section maps that were created by the autonomous driving robot to those of manual surveying. 展开更多
关键词 Pattern matching Location estimation Autonomous driving robot 3D tunnel mapping Underground mine
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面向冶金质检的2D/3D视觉协同检测系统关键算法 被引量:1
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作者 华晋军 王思贤 +1 位作者 唐文明 李树丰 《河北冶金》 2026年第2期65-69,84,共6页
针对热态钢轨质检中高温干扰、缺陷复杂及结构与纹理信息割裂等问题,本文提出并验证了一套面向冶金质检的2D/3D视觉系统检测系统的关键算法。在2D检测方面,提出轻量化DFAM-YOLO-Met(Defect-Focused Attention Module YOLO for Metallur... 针对热态钢轨质检中高温干扰、缺陷复杂及结构与纹理信息割裂等问题,本文提出并验证了一套面向冶金质检的2D/3D视觉系统检测系统的关键算法。在2D检测方面,提出轻量化DFAM-YOLO-Met(Defect-Focused Attention Module YOLO for Metallurgy)模型,通过将特征增强技术与注意力模块与YOLO模型进行耦合,实现对微小缺陷的高效识别。在自建图像集上取得91.6%的mAP和0.89的F1-score,在3D建模方面,设计三阶段点云优化与分段曲率拟合策略,引入热响应补偿机制,实现高温下的在线尺寸精测,误差由±0.28 mm降至±0.12 mm,满足国标≤0.15 mm的要求;在多模态融合方面,构建图-点映射与模块互引机制,实现2D与3D检测结果的联动复核,系统误报率由2.4%降至1.5%,复合缺陷Recall提升11.7%。实测表明,系统可在5 m/s钢轨产线上稳定运行72 h,单帧推理时延11.4 ms,GPU功耗低于15 W,具备良好鲁棒性与部署价值,为冶金行业在高温高速工况下实现智能质检提供了技术路径与实践参考。 展开更多
关键词 热态钢轨 表面检测 map 多模态融合 数据增强 2D/3D视觉协同
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miR-199a-3p对牙髓干细胞炎症因子分泌和细胞凋亡的影响
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作者 李周应 李嘉春 +2 位作者 康京艺 李泉洁 吴煜 《实用口腔医学杂志》 北大核心 2026年第1期31-37,共7页
目的:探究miR-199a-3p对牙髓干细胞(DPSCs)炎症因子分泌和细胞凋亡的影响。方法:利用脂多糖(LPS)刺激DPSCs建立体外牙髓炎细胞模型,lipofectamine2000试剂转染miR-199a-3p mimic/inhibitor及对应阴性对照序列至DPSCs,荧光显微镜下观察... 目的:探究miR-199a-3p对牙髓干细胞(DPSCs)炎症因子分泌和细胞凋亡的影响。方法:利用脂多糖(LPS)刺激DPSCs建立体外牙髓炎细胞模型,lipofectamine2000试剂转染miR-199a-3p mimic/inhibitor及对应阴性对照序列至DPSCs,荧光显微镜下观察转染效率,qRT-PCR技术检测miR-199a-3p的表达量以及炎症相关因子(IL-1α、IL-6、IL-8)和预测靶基因MAP3K4的mRNA表达、ELISA技术检测炎症因子蛋白表达水平、Western blot实验检测细胞凋亡相关因子(BAX、CASPASS-3、BCL-2)和MAP3K4的蛋白表达、流式细胞术检测细胞凋亡数、生信分析方法确定miR-199a-3p潜在靶基因。结果:随LPS刺激的时间延长,DPSCs表达miR-199a-3p逐渐下调而MAP3K4表达上调(P<0.05)。过表达miR-199a-3p下调DPSCs炎症因子的表达和抑制细胞凋亡(P<0.05),敲低miR-199a-3p则反之(P<0.05)。MAP3K4是miR-199a-3p的靶基因之一,过表达miR-199a-3p抑制MAP3K4表达(P<0.05),敲低miR-199a-3p则反之(P<0.05)。论:miR-199a-3p可通过靶向MAP3K4,调控牙髓干细胞的炎症因子分泌和细胞凋亡。 展开更多
关键词 miR-199a-3p 牙髓干细胞 炎症因子 细胞凋亡 map3K4
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Mapping QTLs Affecting Economic Traits on BTA3 in Chinese Holstein with Microsatellite Markers 被引量:1
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作者 QIN Chun-hua CHU Qin +4 位作者 CHU Gui-yan ZHANG Yi ZHANG Qin ZHANG Sheng-li SUN Dong-xiao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第9期1999-2004,共6页
It had been demonstrated that the strong and highly significant quantitative trait locus(QTL) can affect protein percentage on Bos Taurus Autosome 3(BTA3) at the position 52 cM, near the microsatellite DIK4353, wi... It had been demonstrated that the strong and highly significant quantitative trait locus(QTL) can affect protein percentage on Bos Taurus Autosome 3(BTA3) at the position 52 cM, near the microsatellite DIK4353, with the 95% confidence interval spanning from 25 to 57 cM in Chinese Holstein population using QTL-express, MQREML, and GRIDQTL softwares. This study herein focused on such region of fine mapping QTLs for milk production and functional traits with 16 microsatellite markers with coverage of 33 cM between the markers BMS2904 and MB099 on BTA3 in a daughter-designed Chinese Holstein population. A total of 1 298 Holstein cows and 7 sires were genotyped for 16 microsatellites with ABI 3700 DNA sequencer. The variance components QTL linkage analysis(LA) and linkage-disequilibrium(LD) analysis(LA/LD) was performed to map QTLs for 7 traits, i.e., 305-d milk yield, fat yield, protein yield, fat percentage, protein percentage, somatic cell score and persistency of milk yield. Four strong and highly significant QTLs were detected for fat yield, fat percentage, protein percentage and somatic cell score at the position 40, 30, 27 and 26 cM, respectively. Two minor QTLs for milk yield and persistency of milk yield were identified at 42 and 46 cM, respectively. These findings provided a general idea for the fine mapping of the causal mutation for milk production and functional traits on BTA3 in the future. 展开更多
关键词 QTL mapping economic traits microsatellite markers BTA3 Chinese Holstein
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Pedestrian Height Estimation and 3D Reconstruction Using Pixel-resolution Mapping Method Without Special Patterns 被引量:2
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作者 Bing-Xing Wu Suat Utku Ay Ahmed Abdel-Rahim 《International Journal of Automation and computing》 EI CSCD 2019年第4期449-461,共13页
Extracting the three-dimensional(3D)information including location and height of a pedestrian is important for vision-based intelligent traffic monitoring systems.This paper tackles the relationship between pixels′ac... Extracting the three-dimensional(3D)information including location and height of a pedestrian is important for vision-based intelligent traffic monitoring systems.This paper tackles the relationship between pixels′actual size and pixels′spatial resolution through a new method named pixel-resolution mapping(P-RM).The proposed P-RM method derives the equations for pixels′spatial resolutions(XY-direction)and object′s height(Z-direction)in the real world,while introducing new tilt angle and mounting height calibration methods that do not require special calibration patterns placed in the real world.Both controlled laboratory and actual world experiments were performed and reported.The tests on 3D mensuration using proposed P-RM method showed overall better than 98.7%accuracy in laboratory environments and better than 96%accuracy in real world pedestrian height estimations.The 3D reconstructed images for measured points were also determined with the proposed P-RM method which shows that the proposed method provides a general algorithm for 3D information extraction. 展开更多
关键词 Traffic monitoring application spatial resolution pixel-resolution mapping(P-RM)method 3D information pedestrian height estimation
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