以恒定电流场模拟静电场物理实验为对象,综合运用Unity 3D、CATIA、3D Studio Max等技术开发虚拟仿真实验平台,该虚拟仿真实验打破传统实验教学内容的局限,可以实现极间电压范围内任意电压值的测量即测量的电压的连续性,以及在提供实时...以恒定电流场模拟静电场物理实验为对象,综合运用Unity 3D、CATIA、3D Studio Max等技术开发虚拟仿真实验平台,该虚拟仿真实验打破传统实验教学内容的局限,可以实现极间电压范围内任意电压值的测量即测量的电压的连续性,以及在提供实时数据和结果的同时,增加实验数据的处理,帮助学生更好地理解实验原理和效果。利用虚拟技术不仅可有效地缩短学生的学习时间,提高学习效率,也能得到更高的实验成功率与更好的实验效果。展开更多
Many cell-matrix interaction studies have proved that dynamic changes in the extracellular matrix(ECM)are crucial to maintain cellular properties and behaviors.Thus,developing materials that can recapitulate the dynam...Many cell-matrix interaction studies have proved that dynamic changes in the extracellular matrix(ECM)are crucial to maintain cellular properties and behaviors.Thus,developing materials that can recapitulate the dynamic attributes of the ECM is highly desired for threedimensional(3 D)cell culture platforms.To this end,we sought to develop a hydrogel system that would enable dynamic and reversible turning of its mechanical and biochemical properties,thus facilitating the control of cell culture to imitate the natural ECM.Herein,a hydrogel with dynamic mechanics and a biochemistry based on an addition-fragmentation chain transfer(AFCT)reaction was constructed.Thiol-modified hyaluronic acid(HA)and allyl sulfide-modifiedε-poly-L-lysine(EPL)were synthesized to form hydrogels,which were non-swellable and biocompatible.The reversible modulus of the hydrogel was first achieved through the AFCT reaction;the modulus can also be regulated stepwise by changing the dose of UVA irradiation.Dynamic patterning of fluorescent markers in the hydrogel was also realized.Therefore,this dynamically controllable hydrogel has great potential as a 3 D cell culture platform for tissue engineering applications.展开更多
[目的]比较传统手术与应用3D打印技术后手术治疗复杂胫骨平台骨折的临床疗效。[方法]回顾性分析本院2009年3月~2014年7月收治的73例复杂的胫骨平台骨折的患者资料,男51例,女22例,年龄19~57岁,平均38.2岁,骨折按照Schazker分型：Ⅳ型33...[目的]比较传统手术与应用3D打印技术后手术治疗复杂胫骨平台骨折的临床疗效。[方法]回顾性分析本院2009年3月~2014年7月收治的73例复杂的胫骨平台骨折的患者资料,男51例,女22例,年龄19~57岁,平均38.2岁,骨折按照Schazker分型：Ⅳ型33例、Ⅴ型24例、Ⅵ型16例;按照CT分型：双柱骨折51例,三柱骨折22例。其中19例术前采用三维CT重建,3D技术打印实体模型,并在3D模型上模拟手术,术中按术前计划手术（3D技术组）。54例行常规CT扫描、术前准备及手术（常规组）。记录两组患者手术时间、术中出血量、骨折复位质量、手术并发症发生率,术后12个月按照HSS标准进行膝关节功能评分。[结果]所有患者均进行12个月~4年随访（平均24个月）。与常规组相比较,3D技术组手术时间缩短,术中出血量减少,术后12个月膝关节功能HSS评分高,两组间差异有统计学意义（P〈0.05）;术后第2 d拍膝关节X线片,3D组：解剖复位17例,移位〈1 mm 1例,移位〉1 mm 1例,解剖复位率89.4%;常规组：解剖复位35例,移位〈1 mm 15例,移位〉1 mm4例,解剖复位率64.8%,两组间比较差异有统计学意义（P〈0.05）;3D技术组与常规组分别出现创伤性关节炎1例、3例;关节僵直0例、2例;关节不稳1例、3例;下肢深静脉血栓形成0例、1例,两组间比较差异无统计学意义（P〉0.05）。[结论]3D打印技术治疗复杂胫骨平台骨折,可降低手术时间,减少手术出血量,提高骨折复位质量,有效恢复膝关节功能,具有明显的优势。展开更多
文摘以恒定电流场模拟静电场物理实验为对象,综合运用Unity 3D、CATIA、3D Studio Max等技术开发虚拟仿真实验平台,该虚拟仿真实验打破传统实验教学内容的局限,可以实现极间电压范围内任意电压值的测量即测量的电压的连续性,以及在提供实时数据和结果的同时,增加实验数据的处理,帮助学生更好地理解实验原理和效果。利用虚拟技术不仅可有效地缩短学生的学习时间,提高学习效率,也能得到更高的实验成功率与更好的实验效果。
基金financially supported by the National Natural Science Foundation of China(Nos.21803069 and 21975249)。
文摘Many cell-matrix interaction studies have proved that dynamic changes in the extracellular matrix(ECM)are crucial to maintain cellular properties and behaviors.Thus,developing materials that can recapitulate the dynamic attributes of the ECM is highly desired for threedimensional(3 D)cell culture platforms.To this end,we sought to develop a hydrogel system that would enable dynamic and reversible turning of its mechanical and biochemical properties,thus facilitating the control of cell culture to imitate the natural ECM.Herein,a hydrogel with dynamic mechanics and a biochemistry based on an addition-fragmentation chain transfer(AFCT)reaction was constructed.Thiol-modified hyaluronic acid(HA)and allyl sulfide-modifiedε-poly-L-lysine(EPL)were synthesized to form hydrogels,which were non-swellable and biocompatible.The reversible modulus of the hydrogel was first achieved through the AFCT reaction;the modulus can also be regulated stepwise by changing the dose of UVA irradiation.Dynamic patterning of fluorescent markers in the hydrogel was also realized.Therefore,this dynamically controllable hydrogel has great potential as a 3 D cell culture platform for tissue engineering applications.
文摘[目的]比较传统手术与应用3D打印技术后手术治疗复杂胫骨平台骨折的临床疗效。[方法]回顾性分析本院2009年3月~2014年7月收治的73例复杂的胫骨平台骨折的患者资料,男51例,女22例,年龄19~57岁,平均38.2岁,骨折按照Schazker分型：Ⅳ型33例、Ⅴ型24例、Ⅵ型16例;按照CT分型：双柱骨折51例,三柱骨折22例。其中19例术前采用三维CT重建,3D技术打印实体模型,并在3D模型上模拟手术,术中按术前计划手术（3D技术组）。54例行常规CT扫描、术前准备及手术（常规组）。记录两组患者手术时间、术中出血量、骨折复位质量、手术并发症发生率,术后12个月按照HSS标准进行膝关节功能评分。[结果]所有患者均进行12个月~4年随访（平均24个月）。与常规组相比较,3D技术组手术时间缩短,术中出血量减少,术后12个月膝关节功能HSS评分高,两组间差异有统计学意义（P〈0.05）;术后第2 d拍膝关节X线片,3D组：解剖复位17例,移位〈1 mm 1例,移位〉1 mm 1例,解剖复位率89.4%;常规组：解剖复位35例,移位〈1 mm 15例,移位〉1 mm4例,解剖复位率64.8%,两组间比较差异有统计学意义（P〈0.05）;3D技术组与常规组分别出现创伤性关节炎1例、3例;关节僵直0例、2例;关节不稳1例、3例;下肢深静脉血栓形成0例、1例,两组间比较差异无统计学意义（P〉0.05）。[结论]3D打印技术治疗复杂胫骨平台骨折,可降低手术时间,减少手术出血量,提高骨折复位质量,有效恢复膝关节功能,具有明显的优势。
