1,3-Unsaturated fatty acid-2-palmitic acid triacylglycerol(UPU)is a key component of human milk fat substitutes.In this present study,a novel approach was employed to produce UPU by cell factory of Rhodococcus opacus....1,3-Unsaturated fatty acid-2-palmitic acid triacylglycerol(UPU)is a key component of human milk fat substitutes.In this present study,a novel approach was employed to produce UPU by cell factory of Rhodococcus opacus.The resulting UPU displayed a remarkable fatty acid composition,with 37.6%docosahexaenoic acid(DHA),26.3%palmitic acid(P)and 15.6%docosapentaenoic acid(DPA).Notably,palmitic acid dominated the sn-2 position with a content of 51.1%.Furthermore,^(13)C nuclear magnetic resonance analysis revealed the predominant presence of DHA at the sn-1,3 position and palmitic acid at the sn-2 position.Among the triacylglycerol species,β-DHA P DHA(6.6%)andβ-DPA P DHA(6.6%)exhibited the highest levels.The fermentation process achieved a biomass of 2.1 g L^(-1)and an oil content of 39.9%(DCW).The yield of UPU reached 0.89 g L^(-1).This study presented a novel method for manufacturing 1,3-unsaturated fatty acid-2-palmitic acid triacylglycerol enriched with DHA.展开更多
基金gratefully acknowledged Key Research Projects of Colleges and Universities of Henan Province(23A550002)Zhengzhou R&D Special Fund(22ZZRDZX25)Doctoral Scientific Research Start-up Foundation from Henan University of Technology(2021BS015).
文摘1,3-Unsaturated fatty acid-2-palmitic acid triacylglycerol(UPU)is a key component of human milk fat substitutes.In this present study,a novel approach was employed to produce UPU by cell factory of Rhodococcus opacus.The resulting UPU displayed a remarkable fatty acid composition,with 37.6%docosahexaenoic acid(DHA),26.3%palmitic acid(P)and 15.6%docosapentaenoic acid(DPA).Notably,palmitic acid dominated the sn-2 position with a content of 51.1%.Furthermore,^(13)C nuclear magnetic resonance analysis revealed the predominant presence of DHA at the sn-1,3 position and palmitic acid at the sn-2 position.Among the triacylglycerol species,β-DHA P DHA(6.6%)andβ-DPA P DHA(6.6%)exhibited the highest levels.The fermentation process achieved a biomass of 2.1 g L^(-1)and an oil content of 39.9%(DCW).The yield of UPU reached 0.89 g L^(-1).This study presented a novel method for manufacturing 1,3-unsaturated fatty acid-2-palmitic acid triacylglycerol enriched with DHA.
