目的:检测多聚嘧啶结合蛋白3(polypyrimidine tract binding protein 3,PTBP3)在胃癌组织中的表达及定位,探讨其表达水平与根治术后胃癌患者临床病理特征及生存预后的关系。方法:收集90例根治术后胃癌患者组织及对应的癌旁组织标本,制...目的:检测多聚嘧啶结合蛋白3(polypyrimidine tract binding protein 3,PTBP3)在胃癌组织中的表达及定位,探讨其表达水平与根治术后胃癌患者临床病理特征及生存预后的关系。方法:收集90例根治术后胃癌患者组织及对应的癌旁组织标本,制成组织芯片后,采用免疫组织化学(SP染色系统)法检测PTBP3蛋白表达及定位。收集30例配对胃癌及癌旁组织标本,制成cDNA芯片后,采用实时荧光定量PCR法检测PTBP3 mRNA的表达水平。应用SPSS 19.0统计学软件分析PTBP3表达与88例胃癌根治术后患者临床病理特征及生存预后的关系。结果:PTBP3主要定位在细胞核上,胃癌组织中PTBP3蛋白(细胞质P<0.001,细胞核P=0.011)和mRNA(P=0.036)的表达水平均明显高于癌旁组织。多因素分析显示,TNM分期(P=0.031)和癌旁组织中PTBP3表达(P=0.014)为影响根治术后胃癌患者总生存期(overall survival,OS)的独立预后因素;癌旁组织PTBP3蛋白低表达组的中位OS期较高表达组明显延长(68.58 vs 27.27个月,P=0.016)。结论:PTBP3在胃癌中可能具有癌基因的功能,其可能成为胃癌靶向治疗的潜在候选基因。展开更多
Aim:To identify genes related to the human testis development by substrate hybridization technique.Methods:A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal ...Aim:To identify genes related to the human testis development by substrate hybridization technique.Methods:A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal testes and spermatozoa mRNAs by reverse transcription reactions.The differentially expressed genes were sequenced. And a newly identified cullin-3 (CUL-3) transcript variant (designated cul-3b) was bio-informatically analyzed with an online GenBank database.Multi-tissue reverse transcription polymerase chain reaction (RT-PCR) was used to deter- mine the tissue expression profile of cul-3b.Results:Cul-3b,a novel CUL-3 transcript variant,was identified.The expression level of cul-3b in adult testes was 3.79-fold higher than that in fetal ones.Cul-3b differed from cul-3 (including NM_003590 and AY337761) in the opening reading frame and had three internal ribosomal entry sites (IRESes) in the 5'-UTR.These led to a 24 amino acid (aa) truncation at N-terminus of CUL-3b as compared with CUL-3 and a more motivated expression pattern of cul-3b under some strict circumstances.Additionally,cul-3b expressed ubiquitously in human tissues according to multi-tissue RT-PCR.Conclusion:Cul-3b is a novel transcript variant of CUL-3,which may be important not only for the development of human testis but also for that of other organs.展开更多
The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the under...The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.展开更多
文摘目的:检测多聚嘧啶结合蛋白3(polypyrimidine tract binding protein 3,PTBP3)在胃癌组织中的表达及定位,探讨其表达水平与根治术后胃癌患者临床病理特征及生存预后的关系。方法:收集90例根治术后胃癌患者组织及对应的癌旁组织标本,制成组织芯片后,采用免疫组织化学(SP染色系统)法检测PTBP3蛋白表达及定位。收集30例配对胃癌及癌旁组织标本,制成cDNA芯片后,采用实时荧光定量PCR法检测PTBP3 mRNA的表达水平。应用SPSS 19.0统计学软件分析PTBP3表达与88例胃癌根治术后患者临床病理特征及生存预后的关系。结果:PTBP3主要定位在细胞核上,胃癌组织中PTBP3蛋白(细胞质P<0.001,细胞核P=0.011)和mRNA(P=0.036)的表达水平均明显高于癌旁组织。多因素分析显示,TNM分期(P=0.031)和癌旁组织中PTBP3表达(P=0.014)为影响根治术后胃癌患者总生存期(overall survival,OS)的独立预后因素;癌旁组织PTBP3蛋白低表达组的中位OS期较高表达组明显延长(68.58 vs 27.27个月,P=0.016)。结论:PTBP3在胃癌中可能具有癌基因的功能,其可能成为胃癌靶向治疗的潜在候选基因。
文摘Aim:To identify genes related to the human testis development by substrate hybridization technique.Methods:A human testis cDNA microarray was constructed and hybridized with probes prepared from human adult and fetal testes and spermatozoa mRNAs by reverse transcription reactions.The differentially expressed genes were sequenced. And a newly identified cullin-3 (CUL-3) transcript variant (designated cul-3b) was bio-informatically analyzed with an online GenBank database.Multi-tissue reverse transcription polymerase chain reaction (RT-PCR) was used to deter- mine the tissue expression profile of cul-3b.Results:Cul-3b,a novel CUL-3 transcript variant,was identified.The expression level of cul-3b in adult testes was 3.79-fold higher than that in fetal ones.Cul-3b differed from cul-3 (including NM_003590 and AY337761) in the opening reading frame and had three internal ribosomal entry sites (IRESes) in the 5'-UTR.These led to a 24 amino acid (aa) truncation at N-terminus of CUL-3b as compared with CUL-3 and a more motivated expression pattern of cul-3b under some strict circumstances.Additionally,cul-3b expressed ubiquitously in human tissues according to multi-tissue RT-PCR.Conclusion:Cul-3b is a novel transcript variant of CUL-3,which may be important not only for the development of human testis but also for that of other organs.
基金Supported by the National Natural Science Foundation of China(31470107)the National Key Basic Research Program of China(2014CB138302)the China Agriculture Research System(CARS24)
基金financially supported by the National Natural Science Foundation of China(32202560 and 32302470)the Program for Innovative Research Team(in Science and Technology)in University of Henan Province+6 种基金China(21IRTSTHN021)the Natural Science Foundation of HenanChina(232300421112)the Program for Science&Technology Innovation Talents in Universities of Henan ProvinceChina(21HASTIT035)the PhD Research Startup Foundation of Henan University of Science and TechnologyChina(13480068 and 13480067)。
文摘The firmness of table grape berries is a crucial quality parameter. Despite extensive research on postharvest fruit softening, its precise molecular mechanisms remain elusive. To enhance our comprehension of the underlying molecular factors, we initially identified differentially expressed genes(DEGs) by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK) berries at different time points. We then analyzed the sequences to detect alternatively spliced(AS) genes associated with postharvest softening. A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns, with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group. There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries, respectively. Gene Ontology(GO) annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes. Through a comparison between treatment-associated AS genes and subcluster-4 DEGs, we identified eight genes, including Pectinesterase 2(VvPE2, Vitvi15g00704), which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event. The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.
