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Innovative Research on the Interconnection of C-V2X Technology and Hydrogen Refueling Stations
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作者 Wang Gu Yuanyuan Song +1 位作者 Zhihu Zhang Minggang Zheng 《Energy Engineering》 2025年第12期4837-4856,共20页
Driven by the global“dual-carbon”goals,hydrogen fuel cell electric vehicles(FCEVs)are being rapidly promoted as a zero-emission transportation solution.However,their large-scale application is constrained by issues ... Driven by the global“dual-carbon”goals,hydrogen fuel cell electric vehicles(FCEVs)are being rapidly promoted as a zero-emission transportation solution.However,their large-scale application is constrained by issues such as inefficient operation,poor information flow between vehicles and stations,and potential safety hazards,which are caused by insufficient intelligence of hydrogen refueling stations.This study aims to address these problems by deeply integrating Cellular Vehicle-to-Everything(C-V2X)technology with hydrogen refueling stations,thereby building a safe,efficient,and low-carbon hydrogen energy application ecosystem to promote the global transition to zero-carbon transportation.Firstly,through literature review and technical analysis,this study expounds on the core technologies and process flows of current hydrogen refueling stations,aswell as the technical architecture and development evolution of C-V2X technology.Then,based on the analysis of relevant literature,it proposes a“vehicle-road-station-cloud”collaborative architecture that integrates C-V2X with hydrogen refueling stations.Combined with 5G communication and big data technologies,it elaborates on the implementation path for achieving real-time data interaction among hydrogen refueling stations,hydrogen-powered vehicles,and road infrastructure.This interconnection mode enables hydrogen refueling stations to obtain real-time information of surrounding vehicles,which plays an important role in building a safe,efficient,and low-carbon hydrogen energy application ecosystem and promoting the global transition to zero-carbon transportation.Finally,the future development prospects and potential of this scheme are put forward. 展开更多
关键词 Hydrogen fuel cell vehicle hydrogen refueling station C-V2X technology
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CO_(2)-EOR microscopic mechanism under injection-production coupling technology in low-permeability reservoirs
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作者 Zheng Chen Yu-Liang Su +3 位作者 Lei Li Yong-Mao Hao Wen-Dong Wang Chui-Xian Kong 《Petroleum Science》 2025年第2期739-755,共17页
Injection-production coupling(IPC) technology holds substantial potential for boosting oil recovery and enhancing economic efficiency.Despite this potential,discussion on gas injection coupling,especially in relation ... Injection-production coupling(IPC) technology holds substantial potential for boosting oil recovery and enhancing economic efficiency.Despite this potential,discussion on gas injection coupling,especially in relation to microscopic mechanisms,remains relatively sparse.This study utilizes microscopic visualization experiments to investigate the mechanisms of residual oil mobilization under various IPC scenarios,complemented by mechanical analysis at different stages.The research quantitatively assesses the degree of microscopic oil recovery and the distribution of residual oil across different injection-production methods.Findings reveal that during the initial phase of continuous gas injection(CGI),the process closely mimics miscible displacement,gradually transitioning to immiscible displacement as CO_(2)extraction progresses.Compared to CGI,the asynchronous injection-production(AIP) method improved the microscopic oil recovery rate by 6.58%.This enhancement is mainly attributed to significant variations in the pressure field in the AIP method,which facilitate the mobilization of columnar and porous re sidual oil.Furthermo re,the synchronous cycle injection(SCI) method increased microscopic oil recovery by 13.77% and 7.19% compared to CGI and AIP,respectively.In the SCI method,membrane oil displays filame ntary and Karman vo rtex street flow patterns.The dissolved and expanded crude oil te nds to accumulate and grow at the oil-solid interface due to adhesive forces,thereby reducing migration resistance.The study findings provide a theoretical foundation for improving oil recovery in lowpermeability reservoirs. 展开更多
关键词 Low-permeability reservoirs Injection-production coupling Microscopic experimental simulation technology CO_(2)-EOR Mechanical analysis
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Application of Evolutionary Encryption 2D Barcode Generation Technology in Agricultural Product Quality and Safety Traceability System 被引量:4
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作者 Xiaojun ZHONG Zhijie LAI +3 位作者 Yan CHEN Jianxin QIAN Xiaocong HONG Caiyi LI 《Asian Agricultural Research》 2014年第8期76-79,共4页
Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has... Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective. 展开更多
关键词 2D BARCODE technology EVOLUTIONARY ENCRYPTION Trac
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Impact of technology use in type 2 diabetes distress:A systematic review 被引量:2
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作者 Patricia Vieira Renata Kobayasi +2 位作者 Filomena Pereira Isabella Martins Zaia Sandra Umeda Sasaki 《World Journal of Diabetes》 SCIE CAS 2020年第10期459-467,共9页
BACKGROUND Diabetes distress is an important factor in treatment outcomes and results in poor behavioral and biological consequences.Technology has been used in management programs of diabetes to improve communication... BACKGROUND Diabetes distress is an important factor in treatment outcomes and results in poor behavioral and biological consequences.Technology has been used in management programs of diabetes to improve communication between patients and health care providers and to promote education about the disease and its psychological aspects,which can impact the self-efficacy of the programs.However,the true impact of technological approaches on the management of type 2 diabetes distress remains controversial.AIM To investigate the effectiveness of technology interventions on the management of type 2 diabetes distress.METHODS Studies published from 2014 to 2019 were searched in five databases:MEDLINE,PubMed,Library and Information Science Source,Academic Search Ultimate and PsycINFO.The Boolean logic search terms were:(1)T2Diabetes;(2)diabetes distress;and(3)technology OR mobile OR phone OR application OR web.We also systematically searched the reference lists of the included studies and relevant reviews.Randomized controlled trials with technology interventions,type 2 diabetes patients and diabetes distress as the outcome were selected.The Preferred Reporting Items for Systematic Reviews and Meta-Analyses statement was followed.RESULTS Of the 88 studies selected,nine full articles met the inclusion criteria and were subjected to final careful review.On the JADAD scale,one article was classified as having poor quality and eight as having good quality.Six out of nine articles showed that technology interventions had a positive impact on diabetes distress scale scores when compared with the initial data.Among the six articles,five showed a greater reduction in the diabetes distress scores from control interventions.Web-based interventions had good results when users received personalized feedback and routine caregiver support and attention.CONCLUSION Technology interventions can contribute positively to the management of type 2 diabetes distress,especially with a tailored approach in conjunction with caregiver interaction with patients. 展开更多
关键词 technology Type 2 diabetes Diabetes distress Healthcare Systematic review
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The Strategy and Technology Selection for Non-CO_2 Greenhouse Gas Emission Control 被引量:1
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作者 ZHOU Ya-Min FENG Yong-Sheng 《Advances in Climate Change Research》 SCIE 2014年第1期28-33,共6页
The emission control of non-CO2greenhouse gases is conducive to slowing down global warming.It is also helpful in controlling environmental pollution,and beneficial in improving the local health benefits.This paper ai... The emission control of non-CO2greenhouse gases is conducive to slowing down global warming.It is also helpful in controlling environmental pollution,and beneficial in improving the local health benefits.This paper aims at six kinds of non-CO2greenhouse gases under United Nations Framework Convention on Climate Change,namely methane(CH4),nitrous oxide(N2O),hydrofluorocarbons(HFCs),perfluorocarbons(PFCs),sulfur hexafluoride(SF6),and nitrogen trifluoride(NF3).This paper analyzes the emission status and trend of China’s non-CO2greenhouse gases,and provides some technology selections for non-CO2emission reduction.Through strategic policy arrangements and appropriate technology choices,China can gain environmental protection and greenhouse gas control. 展开更多
关键词 non-CO2emission STATUS TREND technology selection
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Technology Improvement for the High Reliability LM-2F Launch Vehicle 被引量:2
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作者 QIN Tong RONG Yi +1 位作者 ZHENG Liwei ZHANG Zhi 《Aerospace China》 2017年第3期38-42,共5页
The Long March 2 F(LM-2F) launch vehicle, the only launch vehicle designed for manned space flight in China, successfully launched the Tiangong 2 space laboratory and the Shenzhou 11 manned spaceship into orbits in 20... The Long March 2 F(LM-2F) launch vehicle, the only launch vehicle designed for manned space flight in China, successfully launched the Tiangong 2 space laboratory and the Shenzhou 11 manned spaceship into orbits in 2016 respectively. In this study, it introduces the technological improvements for enhancing the reliability of the LM-2F launch vehicle in the aspects of general technology, control system, manufacture and ground support system. The LM-2F launch vehicle will continue to provide more contributions to the Chinese Space Station Project with its high reliability and 100% success rate. 展开更多
关键词 LM-2F launch vehicle technology improvement high reliability
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Satellite E2E Network Slicing Based on 5G Technology 被引量:1
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作者 ZHANG Jing WEI Xiao +1 位作者 CHENG Junfeng FENG Xu 《ZTE Communications》 2020年第4期26-33,共8页
We investigate the design of satellite network slicing for the first time to provide customized services for the diversified applications,and propose a novel scheme for satellite end-to-end(E2E) network slicing based ... We investigate the design of satellite network slicing for the first time to provide customized services for the diversified applications,and propose a novel scheme for satellite end-to-end(E2E) network slicing based on 5G technology,which provides a view of common satellite network slicing and supports flexible network deployment between the satellite and the ground.Specifically,considering the limited satellite network resource and the characteristics of the satellite channel,we propose a novel satellite E2E network slicing architecture.Therein,the deployment of the network functions between the satellite and the ground is coordinately considered.Subsequently,the classification and the isolation technologies of satellite network sub-slices are proposed adaptively based on 5G technology to support resource allocation on demand.Then,we develop the management technologies for the satellite E2E network slicing including slicing key performance indicator(KPI) design,slicing deployment,and slicing management.Finally,the analysis of the challenges and future work shows the potential research in the future. 展开更多
关键词 satellite communications E2E network slicing diversified applications 5G technology
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Surveillance of emerging SARS-CoV-2 variants by nanopore technology-based genome sequencing 被引量:1
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作者 J.I.Abeynayake G.P.Chathuranga +1 位作者 M.A.Y.Fernando M.K.Sahoo 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2023年第7期313-320,共8页
Objective:To surveill emerging variants by nanopore technology-based genome sequencing in different COVID-19 waves in Sri Lanka and to examine the association with the sample characteristics,and vaccination status.Met... Objective:To surveill emerging variants by nanopore technology-based genome sequencing in different COVID-19 waves in Sri Lanka and to examine the association with the sample characteristics,and vaccination status.Methods:The study analyzed 207 RNA positive swab samples received to sequence laboratory during different waves.The N gene cut-off threshold of less than 30 was considered as the major inclusion criteria.Viral RNA was extracted,and elutes were subjected to nanopore sequencing.All the sequencing data were uploaded in the publicly accessible database,GISAID.Results:The Omicron,Delta and Alpha variants accounted for 58%,22%and 4%of the variants throughout the period.Less than 1%were Kappa variant and 16%of the study samples remained unassigned.Omicron variant was circulated among all age groups and in all the provinces.Ct value and variants assigned percentage was 100%in Ct values of 10-15 while only 45%assigned Ct value over 25.Conclusions:The present study examined the emergence,prevalence,and distribution of SARS-CoV-2 variants locally and has shown that nanopore technology-based genome sequencing enables whole genome sequencing in a low resource setting country. 展开更多
关键词 Emerging SARS-CoV-2 variants Laboratory surveillance Nanopore technology Genome sequencing Bioinformatics analysis and phylogeny Sociodemographic and sample cutoff(Ct)threshold Global sharing of genomic data/GISAID
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Property Characteristics of a TiB_(2P)/Al Composite Fabricated by Squeeze Casting Technology
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作者 Min ZHAO Gaohui WU Zuoyong DOU Longtao JIANG 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2006年第1期83-86,共4页
TiB2P/Al composite was successfully fabricated by squeeze casting technology.Its mechanical and tribological properties were evaluated .The elimination of Ti-Al intermetallic compound was confirmed by X-ray diffracti... TiB2P/Al composite was successfully fabricated by squeeze casting technology.Its mechanical and tribological properties were evaluated .The elimination of Ti-Al intermetallic compound was confirmed by X-ray diffraction (XRD)studies.At 45% volume fraction ,the bending strength at ambient temperature was 934 MPa.And the fracture modes included ductile failure of Al matrix and brittle fracture of TiB2 pqrticles.In dry sliding wear mode ,severe plastic deformation and adhesive wear were found on te worn surfaces of the SiCP/Al composite .But no obvious characteristics of adhesion or abrasion wear were observed on that of the TiB2P/Al composites .At the stedady stage ,the friction coefficient of the SiCP/Al composite was about 0.6 .While that of TiB2P/Al composite was only only about 0.16-0.17. 展开更多
关键词 TIB2 Aluminum matrix composite Squeeze casting technology PROPERTIES
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EFFECT OF Fe_2O_3 ON WELDING TECHNOLOGY AND MECHANICAL PROPERTIES OF WELD METAL DEPOSITED BY SELF-SHIELDED FLUX CORED WIRE
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作者 YuPing PanChuan +1 位作者 XueJin LiZhengbang 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2005年第1期55-57,共3页
Five experimental self-shielded flux cored wires are fabricated withdifferent amount of Fe_2O_3 in the flux. The effect of Fe_2O_3 on welding technology and mechanicalproperties of weld metals deposited by these wires... Five experimental self-shielded flux cored wires are fabricated withdifferent amount of Fe_2O_3 in the flux. The effect of Fe_2O_3 on welding technology and mechanicalproperties of weld metals deposited by these wires are studied. The results show that with theincrease of Fe_2O_3 in the mix, the melting point of the pretreated mix is increased. LiBaF_3 andBaFe_(12)O_(19), which are very low in inherent moisture, are formed after the pretreatment. Themechanical properties are evaluated to the weld metals. The low temperature notch toughness of theweld metals is increased linearly with the Fe_2O_3 content in the flux due to the balance betweenFe_2O_3 and residual Al in the weld metal. The optimum Fe_2O_3 content in flux is 2.5 percent approx3.5 percent. 展开更多
关键词 Self-shielded flux arc welding Fe_2O_3 Welding technology Mechanicalproperties
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An integrated technology for the absorption and utilization of CO_(2)in alkanolamine solution for the preparation of BaCO_(3)in a high-gravity environment
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作者 Kangrui Nie Ruize Shang +3 位作者 Fuming Miao Liuxiang Wang Youzhi Liu Weizhou Jiao 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第8期117-125,共9页
In this study,an integrated technology is proposed for the absorption and utilization of CO_(2)in alkanolamine solution for the preparation of BaCO_(3)in a high-gravity environment.The effects of absorbent type,high-g... In this study,an integrated technology is proposed for the absorption and utilization of CO_(2)in alkanolamine solution for the preparation of BaCO_(3)in a high-gravity environment.The effects of absorbent type,high-gravity factor,gas/liquid ratio,and initial BaCl2concentration on the absorption rate and amount of CO_(2)and the preparation of BaCO_(3)are investigated.The results reveal that the absorption rate and amount of CO_(2)follow the order of ethyl alkanolamine(MEA)>diethanol amine(DEA)>N-methyldiethanolamine(MDEA),and thus MEA is the most effective absorbent for CO_(2)absorption.The absorption rate and amount of CO_(2)under high gravity are higher than that under normal gravity.Notably,the absorption rate at 75 min under high gravity is approximately 2 times that under normal gravity.This is because the centrifugal force resulting from the high-speed rotation of the packing can greatly increase gas-liquid mass transfer and micromixing.The particle size of BaCO_(3)prepared in the rotating packed bed is in the range of 57.2—89 nm,which is much smaller than that prepared in the bubbling reactor(>100.3 nm),and it also has higher purity(99.6%)and larger specific surface area(14.119 m^(2)·g^(-1)).It is concluded that the high-gravity technology has the potential to increase the absorption and utilization of CO_(2)in alkanolamine solution for the preparation of BaCO_(3).This study provides new insights into carbon emissions reduction and carbon utilization. 展开更多
关键词 High-gravity technology Wet absorption CO_(2)capture Enhanced mass transfer CO_(2)utilization Barium carbonate
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Application and prospects of spatial information technology in CO_(2)sequestration monitoring
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作者 LYU Weifeng LI Yushu +4 位作者 WANG Mingyuan LIN Qianguo JIA Ninghong JI Zemin HE Chang 《Petroleum Exploration and Development》 SCIE 2024年第3期727-740,共14页
This paper systematically reviews the current applications of various spatial information technologies in CO_(2)sequestration monitoring,analyzes the challenges faced by spatial information technologies in CO_(2)seque... This paper systematically reviews the current applications of various spatial information technologies in CO_(2)sequestration monitoring,analyzes the challenges faced by spatial information technologies in CO_(2)sequestration monitoring,and prospects the development of spatial information technologies in CO_(2)sequestration monitoring.Currently,the spatial information technologies applied in CO_(2)sequestration monitoring mainly include five categories:eddy covariance method,remote sensing technology,geographic information system,Internet of Things technology,and global navigation satellite system.These technologies are involved in three aspects:monitoring data acquisition,positioning and data transmission,and data management and decision support.Challenges faced by the spatial information technologies in CO_(2)sequestration monitoring include:selecting spatial information technologies that match different monitoring purposes,different platforms,and different monitoring sites;establishing effective data storage and computing capabilities to cope with the broad sources and large volumes of monitoring data;and promoting collaborative operations by interacting and validating spatial information technologies with mature monitoring technologies.In the future,it is necessary to establish methods and standards for designing spatial information technology monitoring schemes,develop collaborative application methods for cross-scale monitoring technologies,integrate spatial information technologies with artificial intelligence and high-performance computing technologies,and accelerate the application of spatial information technologies in carbon sequestration projects in China. 展开更多
关键词 CO_(2)geological sequestration CO_(2)sequestration monitoring spatial information technology CO_(2)leakage CCUS
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The Route of V-2 Technology Transfer from Germany to the USSR,and on to China
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作者 WANG Fang Yuri M.BATURIN LI Chengzhi 《Chinese Annals of History of Science and Technology》 2021年第2期114-140,共27页
V-2 technology was transferred from Germany to the Union of Soviet Socialist Republics(USSR)and then on to China.The USSR imitated the captured German V-2 rocket,and independently developed the R-2.Later,China imitate... V-2 technology was transferred from Germany to the Union of Soviet Socialist Republics(USSR)and then on to China.The USSR imitated the captured German V-2 rocket,and independently developed the R-2.Later,China imitated the R-2 rocket provided by the USSR,and independently developed the Dong Feng-2(DF-2).The imitation or localization of foreign products is a key stage prior to independent development.Independent development of new models,in turn,is not only indicative of an upgrading of the transferred technology and the mastering of foreign technology,but also an illustration of innovation.Talent is the crucial resource on which technology transfer relies.The administrative system and domestic collaboration network required to coordinate the necessary tasks,including research,experimentation,design,and manufacture were established both in the USSR and in China. 展开更多
关键词 V-2 rocket technology transfer GERMANY the USSR China
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Simulation and optimization for synthetic technology of 2-chloro-4,6-dinitroresorcinol based on back-propagation neural network
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作者 史瑞欣 Huang Yudong 《High Technology Letters》 EI CAS 2007年第3期283-286,共4页
Back-propagation neural network was applied to predict and optimize the synthetic technology of 2-chloro-4,6-dinitroresorcinol. A model was established based on back-propagation neural network using the experimental d... Back-propagation neural network was applied to predict and optimize the synthetic technology of 2-chloro-4,6-dinitroresorcinol. A model was established based on back-propagation neural network using the experimental data of homogeneous design as the training sample set and the technological parameters were optimized by it. The optimal technological parameters are as follows: the reaction time is 4h, the reaction temperature is 80℃, the molar ratio of NaOH to 4,6-dinitro-1,2,3-trichlorobenzene is 5.5:1, the molar ratio of methanol to 4,6-dinitro-1,2,3- trichlorobenzene is 11:1, and the molar ratio of water to 4,6-dinitro-1,2,3-trichlorobenzene is 70:1. Under the optimal conditions, three groups of experiments were performed and the average yield of 2-chloro-4,6-dinitroresorcinol is 96.64%, the absolute error of it with the predicted value is -1.07%. 展开更多
关键词 2-chlom-4 6-dinitroresorcinol synthetic technology OPTIMIZATION back-propagation neural network model constructing
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Signal Processing Circuit Design of Infrared Detection System with SO2 Concentration Based on Correlation Filter Technology
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作者 赵雁雨 姚娜 《Journal of Measurement Science and Instrumentation》 CAS 2011年第4期394-397,共4页
Signals from infrared detector are very weak in SO2 concentration measuring system.In order to improve the sensitivity of detection,combining with filter correlation technology and infrared absorption principle,the we... Signals from infrared detector are very weak in SO2 concentration measuring system.In order to improve the sensitivity of detection,combining with filter correlation technology and infrared absorption principle,the weak signal processing circuit is designed according to correlation detection technology.Under laboratory conditions,system performance of SO2 concentration is tested,and the experimental data are analyzed and processed.Then relationship of SO2 concentration and the measuring voltage is provided to prove that the design improves measuring sensitivity of the system. 展开更多
关键词 SO2 infrared absorption correlation detecting and filtering technology weak signal processing
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Preparation of Al2O3-Supported Nanoscale FeS Based on High-Gravity Technology and Its Application for Removing Chromium (Ⅵ) in Wastewater
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作者 Xiuping Li Wang He +2 位作者 Youzhi Liu Yang Yu Hongyan Shen 《Journal of Environmental Science and Engineering(B)》 2021年第4期129-137,共9页
AbstFeS has an excellent performance in removing heavy metal chromium(Ⅵ)in wastewater due to its good adsorption and reduction.The properties of easy aggregation and oxidization of nano-FeS,however,limit the applicat... AbstFeS has an excellent performance in removing heavy metal chromium(Ⅵ)in wastewater due to its good adsorption and reduction.The properties of easy aggregation and oxidization of nano-FeS,however,limit the applications of FeS in engineering.In this study,one FeS adsorbent supported by Al_(2)O_(3) was prepared using high-gravity technology in IS-RPB(Impinging Stream Rotating Packed Bed)to overcome polymerization and oxidation of nano-FeS.Experimental results showed that FeS was uniformly loaded on the surface and pores of Al_(2)O_(3).The specific surface area of FeS/Al_(2)O_(3) is 125 m2·g^(-1) which is nearly 1.6 times that of pure FeS.The adsorption capacity of FeS/Al_(2)O_(3) for chromium(Ⅵ)is 200 mg·g^(-1),1.4 times that of pure FeS.pH value and ionic strength are strongly correlated with the chromium removal performance of FeS/Al_(2)O_(3).Over 98%of chromium can be removed when pH values of FeS/Al_(2)O_(3) ranged from 4 to 6.Higher adsorption capacity is achieved with higher ionic strength in FeS/Al_(2)O_(3).The FeS/Al_(2)O_(3) maintained more than 95%of the adsorption capacity after being preserved for one month,but only 70%for pure FeS.The removal processes of chromium(Ⅵ)conformes to a pseudo-second-order kinetic model(R2≥0.9986),indicating that the rate-limiting step is a chemical sorption process instead of a mass transfer. 展开更多
关键词 FES Al_(2)O_(3) high-gravity technology chromium(Ⅵ) WASTEWATER
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LM-3B Successfully Launched Communications Technology Test Satellite 2
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作者 GUO Qian 《Aerospace China》 2017年第1期58-58,共1页
At 23:18 Beijing time on January 5,a LM-3B carrier rocket lifted off from the Xichang Satellite Launch Center,sending a communication technology test satellite into its preset orbit.This successful launch was a good s... At 23:18 Beijing time on January 5,a LM-3B carrier rocket lifted off from the Xichang Satellite Launch Center,sending a communication technology test satellite into its preset orbit.This successful launch was a good start for China’s launch missions in 2017.The Communications Technology Test Satellite 2 was developed by the Shanghai Academy of Spaceflight Technology.It is a next-generation telecom- 展开更多
关键词 LM-3B Successfully Launched Communications technology Test Satellite 2 TEST DATA
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利用2b-RAD测序结合HRM分析技术开发与梨矮生性状相关的DNA分子标记 被引量:4
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作者 肖玉雄 王彩虹 +3 位作者 田义轲 杨绍兰 李鼎立 张海月 《中国农业科学》 CAS CSCD 北大核心 2017年第15期3006-3012,共7页
【目的】果树的矮生性状是一种重要的农艺性状,对果树的集约化栽培具有重要意义。来自西洋梨实生变异品种‘Le Nain Vert’的矮生性状受控于一个单显性基因Pc Dw,目前关于该基因的序列信息等还不清楚。本研究的目的是开发与其紧密连锁的... 【目的】果树的矮生性状是一种重要的农艺性状,对果树的集约化栽培具有重要意义。来自西洋梨实生变异品种‘Le Nain Vert’的矮生性状受控于一个单显性基因Pc Dw,目前关于该基因的序列信息等还不清楚。本研究的目的是开发与其紧密连锁的DNA分子标记,为鉴定该基因提供依据。【方法】根据分离群体分组分析的原理,以‘矮生梨’ב茌梨’和‘2-3’ב绿宝石’2个F1杂交分离群体为试材,应用IIB型限制性内切酶的RAD技术(Restriction association site DNA,2b-RAD)对2对矮生型/普通型对比基因池进行基因组测序分析,在对比基因池间筛选出单核苷酸多态性(Single nucleotide polymorphism,SNP)位点,从中筛查出位于Pc Dw定位染色体上的SNPs,用高分辨率熔解曲线分析技术(High-resolution melting analysis,HRM)在群体上进一步检测验证,以确定其与Pc Dw位点的连锁关系。【结果】对4个样本(即4个对比基因池)的2b-RAD标签测序文库的测序结果共产生67 186 260条reads,平均每个样本测序reads数为16 796 565。将原始reads进行质量过滤后的统计结果表明,每个样本获得平均unique标签数目为86 810,平均测序深度为77×,该测序深度能够达到准确分型的标准。SOAP软件定位结果表明,4个测序文库中含有酶切位点的高质量reads占测序原始reads的70%以上,表明测序质量较好。在来自2个不同群体的矮生型基因池与普通型基因池间进行对比分析,初步筛选出SNP位点1 317个,其中有8个位于PcDw的定位染色体scaffold00074上。用HRM技术对这8个SNP标记在群体上的进一步检测结果表明,在‘矮生梨’ב茌梨’群体上有2个SNP标记、在‘2-3’ב绿宝石’群体上有4个SNP标记表现出与Pc Dw位点共分离的特性,根据其扩增子的熔解曲线形状差异,可有效区分矮生型和普通型表型。在来自‘矮生梨’ב茌梨’群体的215个杂种后代和来自‘2-3’ב绿宝石’群体的168个杂种后代中,未发现有标记与性状的重组类型。【结论】2b-RAD测序技术与HRM分析技术相结合,进行果树重要农艺性状分子标记的开发,是一种行之有效的方法。基于这一策略,本研究鉴定获得了4个与西洋梨矮生性状单显性基因Pc Dw连锁的SNP标记。 展开更多
关键词 矮生性状 PcDw SNP标记 2b-rad HRM
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我国不同地区猪源致泻性大肠杆菌的多重PCR鉴定及2b-RAD序列分析 被引量:2
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作者 李月华 赵建梅 +5 位作者 黄秀梅 王娟 曲志娜 赵格 刘俊辉 王君玮 《中国动物检疫》 CAS 2020年第9期46-50,68,共6页
为了解我国不同地区屠宰生猪肉品中致泻性大肠杆菌污染情况及系统进化关系,降低由其带来的公共卫生危害,将近几年分离自华北、华东、华中及西南地区猪屠体表面的283株大肠杆菌进行了致泻性大肠杆菌多重PCR鉴定及2b-RAD测序分型。多重PC... 为了解我国不同地区屠宰生猪肉品中致泻性大肠杆菌污染情况及系统进化关系,降低由其带来的公共卫生危害,将近几年分离自华北、华东、华中及西南地区猪屠体表面的283株大肠杆菌进行了致泻性大肠杆菌多重PCR鉴定及2b-RAD测序分型。多重PCR鉴定结果显示,共检出36株致泻性大肠杆菌,检出率为12.7%,其中西南地区检出率(26.92%)高于华北(13.33%)、华东(10.57%)和华中(10.81%)地区;5种致泻类型中,产肠毒素大肠杆菌(ETEC)、肠致泻性大肠杆菌(EPEC)、肠聚集性大肠杆菌(EAEC)、肠出血性大肠杆菌(EHEC)和肠侵袭性大肠杆菌(EIEC)的占比分别为33.33%、30.56%、25.00%、5.56%和5.56%。将致泻性大肠杆菌进行2b-RAD测序,种群聚类分析显示,这36株致泻菌可分为8个亚群;系统发生树分析显示,亲缘关系较近菌株的致泻类型基本一致,分离时间也比较接近,而且来自同一地区菌株的亲缘关系相对较近。可见,我国屠宰生猪肉品中污染的致泻性大肠杆菌以ETEC、EPEC和EAEC类型为主,其流行分布有一定的地域差异,且其亲缘关系呈一定的时空特异性。本研究为精准防控生猪屠宰环节致泻性大肠杆菌污染,保障肉品卫生安全提供了科学数据和技术支撑。 展开更多
关键词 致泻性大肠杆菌 生猪 多重PCR 2b-rad测序
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基于2b-RAD技术的糯玉米品种‘沪玉糯3号’特异SNP分子标记开发 被引量:1
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作者 卢媛 韩晴 +2 位作者 王义发 施标 沈雪芳 《植物生理学报》 CAS CSCD 北大核心 2020年第3期573-582,共10页
利用2b-RAD (type IIB endonucleases restriction-site associated DNA)技术对糯玉米品种‘沪玉糯3号’及其亲本进行简化基因组测序。通过与玉米‘B73’参考基因组序列进行比对,在3个糯玉米样品的共同测序标签内筛选出20 498个单核苷... 利用2b-RAD (type IIB endonucleases restriction-site associated DNA)技术对糯玉米品种‘沪玉糯3号’及其亲本进行简化基因组测序。通过与玉米‘B73’参考基因组序列进行比对,在3个糯玉米样品的共同测序标签内筛选出20 498个单核苷酸多态性(single nucleotide polymorphism, SNP)位点,并基于RADtyping分型策略对它们进行基因型分型。对其中20 333个位于玉米10条染色体上的SNP位点的基因型进行分析,开发出6个可用于‘沪玉糯3号’品种特异性鉴定的SNP分子标记。 展开更多
关键词 糯玉米‘沪玉糯3号’ 2b-rad SNP 品种特异性
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