目的检测并分析母系突变的亲子鉴定案件,研究基因突变的意义和影响。方法采用Microreader^(TM)21D ID System试剂盒、Microreader^(TM)23SP ID System试剂盒和Huaxia^(TM)Platinum扩增试剂盒对1例疑似存在遗传异常的家系进行常染色体ST...目的检测并分析母系突变的亲子鉴定案件,研究基因突变的意义和影响。方法采用Microreader^(TM)21D ID System试剂盒、Microreader^(TM)23SP ID System试剂盒和Huaxia^(TM)Platinum扩增试剂盒对1例疑似存在遗传异常的家系进行常染色体STR检测分析。结果D2S441基因座发生了母系突变,通过增加位点辅助检测,分别用二联体、三联体公式计算达到认定标准。结论母系突变的发生概率较低,D2S441基因座发生母系突变的检测研究提供数据材料。展开更多
Seed maturation is a critical development transition and it largely affects the final yield and quality of crops.Abscisic acid(ABA)-activated sucrose-non-fermentation kinase subfamily 2(SnRK2s)constitute a well-known ...Seed maturation is a critical development transition and it largely affects the final yield and quality of crops.Abscisic acid(ABA)-activated sucrose-non-fermentation kinase subfamily 2(SnRK2s)constitute a well-known regulatory network that modulate seed maturation in Arabidopsis;however,the underlying genetic and regulatory mechanisms in cereal crops remain largely unknown.Here,we found that ABA levels exhibited two distinct peaks during kernel development in maize,corresponding to the lag and maturation phase,respectively.Integrated transcriptome and proteome profiling of kernels treated with exogenous ABA at the pre-maturation stage suggested that the second peak of ABA acts as a trigger for kernel maturation program.Knockout of ZmSnRK2s demonstrated that subclassⅢZmSnRK2s are required for kernel maturation in maize,and the loss-of-function of subclassⅢZmSnRK2s showed a disruption in kernel dehydration and dormancy.We identified a conserved ABA–SnRK2–b ZIP signaling pathway mediating this process in maize.Additionally,ZmSnRK2.10 overexpression accelerates kernel dehydration during maturity,achieving reduced kernel moisture content(KMC)at physiological maturity(PM).Overall,our findings establish ABA-activated SnRK2s as central regulators of kernel maturation in maize and provide valuable genetic resources for breeding maize varieties with low moisture content at harvest.展开更多
文摘目的检测并分析母系突变的亲子鉴定案件,研究基因突变的意义和影响。方法采用Microreader^(TM)21D ID System试剂盒、Microreader^(TM)23SP ID System试剂盒和Huaxia^(TM)Platinum扩增试剂盒对1例疑似存在遗传异常的家系进行常染色体STR检测分析。结果D2S441基因座发生了母系突变,通过增加位点辅助检测,分别用二联体、三联体公式计算达到认定标准。结论母系突变的发生概率较低,D2S441基因座发生母系突变的检测研究提供数据材料。
基金supported by the National Natural Science Foundation of China(32201696)the Natural Science Foundation of Sichuan Province(23NSFSC4071)。
文摘Seed maturation is a critical development transition and it largely affects the final yield and quality of crops.Abscisic acid(ABA)-activated sucrose-non-fermentation kinase subfamily 2(SnRK2s)constitute a well-known regulatory network that modulate seed maturation in Arabidopsis;however,the underlying genetic and regulatory mechanisms in cereal crops remain largely unknown.Here,we found that ABA levels exhibited two distinct peaks during kernel development in maize,corresponding to the lag and maturation phase,respectively.Integrated transcriptome and proteome profiling of kernels treated with exogenous ABA at the pre-maturation stage suggested that the second peak of ABA acts as a trigger for kernel maturation program.Knockout of ZmSnRK2s demonstrated that subclassⅢZmSnRK2s are required for kernel maturation in maize,and the loss-of-function of subclassⅢZmSnRK2s showed a disruption in kernel dehydration and dormancy.We identified a conserved ABA–SnRK2–b ZIP signaling pathway mediating this process in maize.Additionally,ZmSnRK2.10 overexpression accelerates kernel dehydration during maturity,achieving reduced kernel moisture content(KMC)at physiological maturity(PM).Overall,our findings establish ABA-activated SnRK2s as central regulators of kernel maturation in maize and provide valuable genetic resources for breeding maize varieties with low moisture content at harvest.
