Bruchid is a serious insect pest of mungbean [Vigna radiata （L.） Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglti 1, from the World Veget...Bruchid is a serious insect pest of mungbean [Vigna radiata （L.） Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglti 1, from the World Vegetable Center, Asian Vegetable Research and Development Center （AVRDC）. Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. To find molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR/STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus （named as Br2）. Further analysis suggested that the genetic distances between these two markers and Br2 were 11.0 and 5.8 cM, respectively.展开更多
基金supported by the National Natural Science Foundation of China(30170635)Crop Germplasm Protection,China(NB04-22-12)National Facilities and Information Infrastructure for Scinece and Technology,China(2004DKA30380-08,National Key Technology Support Plan,China(2006BAD02B08),Science and Technology of Agricultural Vocation,China(nyhyzx07-017).
文摘Bruchid is a serious insect pest of mungbean [Vigna radiata （L.） Wilczek] and can inflict serious loss. A resistant variety from India, V2709, was crossed with a susceptible variety, Zhonglti 1, from the World Vegetable Center, Asian Vegetable Research and Development Center （AVRDC）. Segregation of the F2, BC1F1, and F3 populations showed that bruchid resistance of V2709 is controlled by a single dominant locus. To find molecular marker linked with the resistant locus, 63 randomly amplified polymorphic DNA markers and 113 sets of SSR/STS primers were used in a bulked segregant analysis. Two of the markers, OPC-06 and STSbr2, were found to be linked with the locus （named as Br2）. Further analysis suggested that the genetic distances between these two markers and Br2 were 11.0 and 5.8 cM, respectively.
