A型流感病毒(influenza A virus,IAV)完全依赖于宿主蛋白完成自身的生命周期。然而,调控其膜蛋白转运的分子机制尚不清楚。2025年7月8日,华中农业大学周红波教授带领的研究团队在Nature Communications上发表了题为“Rab27a regulates t...A型流感病毒(influenza A virus,IAV)完全依赖于宿主蛋白完成自身的生命周期。然而,调控其膜蛋白转运的分子机制尚不清楚。2025年7月8日,华中农业大学周红波教授带领的研究团队在Nature Communications上发表了题为“Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane”的研究论文,揭示了Rab27a及其效应分子SYTL1和SYTL4通过促进流感病毒膜蛋白转运从而有利于病毒粒子出芽的分子机制。展开更多
Tiller number represents a critical agronomic trait determining sugarcane yield.Strigolactones(SLs) are plant hormones regulating plant architecture.D27,an essential enzyme in the SL biosynthetic pathway,catalyzes a r...Tiller number represents a critical agronomic trait determining sugarcane yield.Strigolactones(SLs) are plant hormones regulating plant architecture.D27,an essential enzyme in the SL biosynthetic pathway,catalyzes a reversible isomerization reaction.ScD27.2,the D27 homolog in sugarcane,contains abiotic stress-responsive elements in its promoter,suggesting its potential importance in SL biosynthesis and stress tolerance.ScD27.2 potentially optimizes sugarcane agronomic traits,particularly tiller number and yield.Understanding its mechanisms will advance the development of high-yielding,stresstolerant sugarcane varieties.To investigate the role of D27 in sugarcane tillering,the key carotene isomerase gene ScD 27.2 was silenced(via RNAi) and overexpressed(OE) in sugarcane cultivar ‘XTT22' plantlets.ScD27-RNAi-2 sugarcane exhibited decreased ScD27.2 expression and increased tiller numbers compared to the wild type ‘XTT22'.Conversely,overexpression lines(ScD27-OE-1,ScD27-OE-5,and ScD27-OE-9) showed increased ScD27.2 expression and decreased tiller numbers.ScD27-OE-9 demonstrated notable lateral bud germination,while ScD27-RNAi-2 exhibited reduced drought tolerance.Under normal light and water management conditions,transgenic sugarcane plants showed significant differences in tiller number and plant height.During extended drought conditions,ScD27-RNAi-2 height was significantly reduced compared to wild type ‘XTT22' and ScD27-OE-9,manifesting a dwarf,multi-tiller phenotype.Additionally,ScD27-RNAi-2 showed significantly reduced SLs content.These findings demonstrate that ScD27.2 regulates tillering under drought stress,likely through SL biosynthesis,and that its drought response may be mediated by the transcription factor ScMYB44.展开更多
文摘A型流感病毒(influenza A virus,IAV)完全依赖于宿主蛋白完成自身的生命周期。然而,调控其膜蛋白转运的分子机制尚不清楚。2025年7月8日,华中农业大学周红波教授带领的研究团队在Nature Communications上发表了题为“Rab27a regulates the transport of influenza virus membrane proteins to the plasma membrane”的研究论文,揭示了Rab27a及其效应分子SYTL1和SYTL4通过促进流感病毒膜蛋白转运从而有利于病毒粒子出芽的分子机制。
基金funded by the National Natural Science Foundation of China (31860405)the National Key Laboratory for Tropical Crop Breeding,China (NKLTCB-YAAS-2024-S05)+4 种基金the Yunnan Provincial Modern Agricultural Technology System,Chinathe Yunnan Provincial Science and Technology Innovation TalentProgram,China (202305AD160041)the Yunnan Provincial “Xingdian Talent Support Program”,Chinathe Yunnan Haizhi Station for Sugarcane Research Institute of Yunnan Academy of Agricultural Sciences,China (HHZ202201)the Yunnan Provincial Key Research and Development Program,China (202403AM140025)。
文摘Tiller number represents a critical agronomic trait determining sugarcane yield.Strigolactones(SLs) are plant hormones regulating plant architecture.D27,an essential enzyme in the SL biosynthetic pathway,catalyzes a reversible isomerization reaction.ScD27.2,the D27 homolog in sugarcane,contains abiotic stress-responsive elements in its promoter,suggesting its potential importance in SL biosynthesis and stress tolerance.ScD27.2 potentially optimizes sugarcane agronomic traits,particularly tiller number and yield.Understanding its mechanisms will advance the development of high-yielding,stresstolerant sugarcane varieties.To investigate the role of D27 in sugarcane tillering,the key carotene isomerase gene ScD 27.2 was silenced(via RNAi) and overexpressed(OE) in sugarcane cultivar ‘XTT22' plantlets.ScD27-RNAi-2 sugarcane exhibited decreased ScD27.2 expression and increased tiller numbers compared to the wild type ‘XTT22'.Conversely,overexpression lines(ScD27-OE-1,ScD27-OE-5,and ScD27-OE-9) showed increased ScD27.2 expression and decreased tiller numbers.ScD27-OE-9 demonstrated notable lateral bud germination,while ScD27-RNAi-2 exhibited reduced drought tolerance.Under normal light and water management conditions,transgenic sugarcane plants showed significant differences in tiller number and plant height.During extended drought conditions,ScD27-RNAi-2 height was significantly reduced compared to wild type ‘XTT22' and ScD27-OE-9,manifesting a dwarf,multi-tiller phenotype.Additionally,ScD27-RNAi-2 showed significantly reduced SLs content.These findings demonstrate that ScD27.2 regulates tillering under drought stress,likely through SL biosynthesis,and that its drought response may be mediated by the transcription factor ScMYB44.
