Although mechanism of symbiosis between arbuscular mycorrhizal fungi (AMF) and host plants has been investigated by genetic analysis, very little knowledge has been obtained because genome analysis of AMF is not perfe...Although mechanism of symbiosis between arbuscular mycorrhizal fungi (AMF) and host plants has been investigated by genetic analysis, very little knowledge has been obtained because genome analysis of AMF is not perfect yet. Thus, we tried to develop mass purification of proteins using preparative chromatography in order to accelerate roteomic analysis of proteins related to mycorrhizal symbiosis, such as 24 and 53 kDa proteins. In particular, our data showed that 53 kDa proteins would be restrictively expressed when mycorrhizal fungi and host plants were stressed. However, 24 kDa proteins, which appear to be a usable indicator for the existence of various my-corrhizal fungi, were habitually detected in not only AMF but also other mycorrhizal fungi such as ectomycorrhizal fungi (EF). Moreover, we discovered new preparative chromatographical techniques for isolation and mass purification of those proteins. We are convinced that this chromato-graphical technique will greatly contribute to proteomic approach of mycorrhizal symbiosis.展开更多
The photosynthetic apparatus of plant is localised in chloroplasts. During the photosynthesis, the absorption, transfer and conversion, of light energy, water-splitting reaction and photophosphorylation take place in ...The photosynthetic apparatus of plant is localised in chloroplasts. During the photosynthesis, the absorption, transfer and conversion, of light energy, water-splitting reaction and photophosphorylation take place in the chlorophyll-protein complexes of geometrically arranged pigments and proteins embedded in thylakoid membranes. The turnover of thylakoid membrane proteins requires various proteolytic activities. The Dl reaction center protein (Q_B-binding protein) has been demonstrated to require展开更多
文摘Although mechanism of symbiosis between arbuscular mycorrhizal fungi (AMF) and host plants has been investigated by genetic analysis, very little knowledge has been obtained because genome analysis of AMF is not perfect yet. Thus, we tried to develop mass purification of proteins using preparative chromatography in order to accelerate roteomic analysis of proteins related to mycorrhizal symbiosis, such as 24 and 53 kDa proteins. In particular, our data showed that 53 kDa proteins would be restrictively expressed when mycorrhizal fungi and host plants were stressed. However, 24 kDa proteins, which appear to be a usable indicator for the existence of various my-corrhizal fungi, were habitually detected in not only AMF but also other mycorrhizal fungi such as ectomycorrhizal fungi (EF). Moreover, we discovered new preparative chromatographical techniques for isolation and mass purification of those proteins. We are convinced that this chromato-graphical technique will greatly contribute to proteomic approach of mycorrhizal symbiosis.
基金Project supported by the National Natural Science Foundation of China and Grants-in-Aid of Sichuan University.
文摘The photosynthetic apparatus of plant is localised in chloroplasts. During the photosynthesis, the absorption, transfer and conversion, of light energy, water-splitting reaction and photophosphorylation take place in the chlorophyll-protein complexes of geometrically arranged pigments and proteins embedded in thylakoid membranes. The turnover of thylakoid membrane proteins requires various proteolytic activities. The Dl reaction center protein (Q_B-binding protein) has been demonstrated to require
