AIM： To analyze the DNA copy number of target genes NF2, TIMP3, ST13, TOB2, BIK, and TP and the reference microsatellite markers D22S283, D22S423, and D22S274 mapped on 22q12-qter in liver fluke related cholangiocarc...AIM： To analyze the DNA copy number of target genes NF2, TIMP3, ST13, TOB2, BIK, and TP and the reference microsatellite markers D22S283, D22S423, and D22S274 mapped on 22q12-qter in liver fluke related cholangiocarcinoma （CCA） and define its correlation with clinical parameters. METHODS： Quantitative real time PCR （qPCR） was used for determining allelic imbalances in 65 liver fluke related CCA tissues. Statistical correlations between allelic imbalances and clinicopathological parameters, i.e. age, sex, tumor stage, histological type, blood vessel invasion, nerve invasion and lymphatic invasion were evaluated by means of the X^2 test. Cox regression analysis was used for determining patient＇s survival. RESULTS： Amplifications of the TP （22q13.33）, TOB2 （22q13.2-13.31）, D22S283 （22q12.3）, TIMP3 （22q12.3） and NF2 （22q12.2） were found in 35 （53.8%）, 28 （43.1%）, 27 （41.5%）, 24 （36.9%）, and 24 （36.9%）, respectively. Losses at the D22S423 （22q13.1-13.2）and BIK （22q13.31） were detected in 26 （40%） and 23 （35.4%）, respectively. Significant correlations were observed between lymphatic invasion and allelic losses of BIK （P = 0.025） and D22S283 （P = 0.041）. Univariate and multivariate Cox regression analysis revealed D22S283 amplification as an independent predictor of good prognosis （P = 0.006, death hazard ratio = 0.411, 95% CI = 0.217-0.779） and blood vessel invasion as an independent poor prognostic factor （P = 0.042, death hazard ratio = 1.911, 95% CI = 1.022-3.571） in CCA patients. CONCLUSION： This study provides evidence for the involvement of gene amplification and deletion on chromosome 22q in liver fluke related CCA, This is the first report of D22S283 amplification as an independent indicator of favorable prognosis in liver fluke related CCA.展开更多
Homobrassinolide (2) and (22S, 23S)-22,23-epi-homobrassinolide (4) were synthesized fromstigmasterol in seven steps respectively in 4.7% and 24.1% overall yields. The ratio of 2 to 4is 1:5. The key step is the highly ...Homobrassinolide (2) and (22S, 23S)-22,23-epi-homobrassinolide (4) were synthesized fromstigmasterol in seven steps respectively in 4.7% and 24.1% overall yields. The ratio of 2 to 4is 1:5. The key step is the highly regioselective formation of the B--homo-7-oxa-lactone ringby oxidation of an enol silyl ether with 3--chloro-peroxybenzoic acid.展开更多
In this paper,obtainment of 3 by simultaneous inversion of configurations of C_(22),C_(23) in 2 via its ditosylate or dimesylate was unsuccessful,leading to elimination products instead.However, monotosylate of 2 can ...In this paper,obtainment of 3 by simultaneous inversion of configurations of C_(22),C_(23) in 2 via its ditosylate or dimesylate was unsuccessful,leading to elimination products instead.However, monotosylate of 2 can be readily obtained with exclusive regioselectivity,which in turn gave a single epoxide 7b Hydrolysis of 7b gave 3 and 2 is a ratio of 2:1,with a combined yield of 80%.The recovered 2 can of course be recycled again.展开更多
Three compounds were obtained from the mycelia of an endophytic fungus Gliocladium sp. (designated as strain F) of Taxus chinensis (Pilg.) Rehd. growing in Fujian Province, China. Their structures were determined on t...Three compounds were obtained from the mycelia of an endophytic fungus Gliocladium sp. (designated as strain F) of Taxus chinensis (Pilg.) Rehd. growing in Fujian Province, China. Their structures were determined on the basis of spectral analysis. (20S,22S)-4a-homo-22-hydroxy-4-oxaergosta-7,24(28)-dien-3-one was a novel compound. 4,8,12,16-tetramethyl-1,5,9,13-tetraoxacyclohexadecane-2,6,10,14-tetraone was firstly isolated from the genus ofGliocladium and 6,9-epoxyergosta-7,22-dien-3-ol was firstly obtained from the strain F.展开更多
The hydrothermal signatures of mid-ocean ridge sediments are crucial geochemical data providing insights into investigating hydrothermal anomalies and locating seafloor massive sulfide deposits.This paper outlines the...The hydrothermal signatures of mid-ocean ridge sediments are crucial geochemical data providing insights into investigating hydrothermal anomalies and locating seafloor massive sulfide deposits.This paper outlines the geochemical features of 24 surface sediments and one sediment core(26 V-GC 01,294 cm)along the South Mid-Atlantic Ridge(SMAR)from 18°S to 22°S,an area where hydrothermal active fields have yet to be discovered.The surface sediments mainly consist of biogenic carbonates,aluminosilicates,and hydrothermal Fe-Mn(oxy)oxides.The core sediments primarily comprise organic matter,detrital materials,hydrothermal components,and substances scavenged from seawater.The rare Earth element(REE)patterns suggest the presence of hydrothermal contributions within the surface and core sediments.The enrichment factors for Fe,Mn,Cu,and Zn in surface sediments suggest these metals are concentrated at the 19°S,21°S,and 21.5°S segments,further indicating their potential as hydrothermal active fields.Downcore variations of Fe,Mn,P,Cu,Pb,V,and Co suggest at least six episodes of hydrothermal activity.The impact of hydrothermal processes on the sediments from SMAR 18°S to 22°S indicates that the study area has the potential to host a significant number of hydrothermal active fields.展开更多
基金Supported by The Thailand Research Fund through The Royal Golden Jubilee PhD Program, Grant No. PHD/0037/2544 for Thanasai J and Limpaiboon T
文摘AIM： To analyze the DNA copy number of target genes NF2, TIMP3, ST13, TOB2, BIK, and TP and the reference microsatellite markers D22S283, D22S423, and D22S274 mapped on 22q12-qter in liver fluke related cholangiocarcinoma （CCA） and define its correlation with clinical parameters. METHODS： Quantitative real time PCR （qPCR） was used for determining allelic imbalances in 65 liver fluke related CCA tissues. Statistical correlations between allelic imbalances and clinicopathological parameters, i.e. age, sex, tumor stage, histological type, blood vessel invasion, nerve invasion and lymphatic invasion were evaluated by means of the X^2 test. Cox regression analysis was used for determining patient＇s survival. RESULTS： Amplifications of the TP （22q13.33）, TOB2 （22q13.2-13.31）, D22S283 （22q12.3）, TIMP3 （22q12.3） and NF2 （22q12.2） were found in 35 （53.8%）, 28 （43.1%）, 27 （41.5%）, 24 （36.9%）, and 24 （36.9%）, respectively. Losses at the D22S423 （22q13.1-13.2）and BIK （22q13.31） were detected in 26 （40%） and 23 （35.4%）, respectively. Significant correlations were observed between lymphatic invasion and allelic losses of BIK （P = 0.025） and D22S283 （P = 0.041）. Univariate and multivariate Cox regression analysis revealed D22S283 amplification as an independent predictor of good prognosis （P = 0.006, death hazard ratio = 0.411, 95% CI = 0.217-0.779） and blood vessel invasion as an independent poor prognostic factor （P = 0.042, death hazard ratio = 1.911, 95% CI = 1.022-3.571） in CCA patients. CONCLUSION： This study provides evidence for the involvement of gene amplification and deletion on chromosome 22q in liver fluke related CCA, This is the first report of D22S283 amplification as an independent indicator of favorable prognosis in liver fluke related CCA.
文摘Homobrassinolide (2) and (22S, 23S)-22,23-epi-homobrassinolide (4) were synthesized fromstigmasterol in seven steps respectively in 4.7% and 24.1% overall yields. The ratio of 2 to 4is 1:5. The key step is the highly regioselective formation of the B--homo-7-oxa-lactone ringby oxidation of an enol silyl ether with 3--chloro-peroxybenzoic acid.
文摘In this paper,obtainment of 3 by simultaneous inversion of configurations of C_(22),C_(23) in 2 via its ditosylate or dimesylate was unsuccessful,leading to elimination products instead.However, monotosylate of 2 can be readily obtained with exclusive regioselectivity,which in turn gave a single epoxide 7b Hydrolysis of 7b gave 3 and 2 is a ratio of 2:1,with a combined yield of 80%.The recovered 2 can of course be recycled again.
文摘Three compounds were obtained from the mycelia of an endophytic fungus Gliocladium sp. (designated as strain F) of Taxus chinensis (Pilg.) Rehd. growing in Fujian Province, China. Their structures were determined on the basis of spectral analysis. (20S,22S)-4a-homo-22-hydroxy-4-oxaergosta-7,24(28)-dien-3-one was a novel compound. 4,8,12,16-tetramethyl-1,5,9,13-tetraoxacyclohexadecane-2,6,10,14-tetraone was firstly isolated from the genus ofGliocladium and 6,9-epoxyergosta-7,22-dien-3-ol was firstly obtained from the strain F.
基金Supported by the China Ocean Mineral Resources R&D Association(No.DY135-S2-2)the Basic Scientific Fund for National Public Research Institutes of China(No.2021Q01)the National Natural Science Foundation of China(Nos.42106080,42006180,42276080)。
文摘The hydrothermal signatures of mid-ocean ridge sediments are crucial geochemical data providing insights into investigating hydrothermal anomalies and locating seafloor massive sulfide deposits.This paper outlines the geochemical features of 24 surface sediments and one sediment core(26 V-GC 01,294 cm)along the South Mid-Atlantic Ridge(SMAR)from 18°S to 22°S,an area where hydrothermal active fields have yet to be discovered.The surface sediments mainly consist of biogenic carbonates,aluminosilicates,and hydrothermal Fe-Mn(oxy)oxides.The core sediments primarily comprise organic matter,detrital materials,hydrothermal components,and substances scavenged from seawater.The rare Earth element(REE)patterns suggest the presence of hydrothermal contributions within the surface and core sediments.The enrichment factors for Fe,Mn,Cu,and Zn in surface sediments suggest these metals are concentrated at the 19°S,21°S,and 21.5°S segments,further indicating their potential as hydrothermal active fields.Downcore variations of Fe,Mn,P,Cu,Pb,V,and Co suggest at least six episodes of hydrothermal activity.The impact of hydrothermal processes on the sediments from SMAR 18°S to 22°S indicates that the study area has the potential to host a significant number of hydrothermal active fields.
