AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma ...AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.展开更多
<div style="text-align:justify;"> Bonding is used widely when the optical glasses are connected with metal structures. It plays a very important role in the design of space telescope. Improper bonding ...<div style="text-align:justify;"> Bonding is used widely when the optical glasses are connected with metal structures. It plays a very important role in the design of space telescope. Improper bonding may induce great surface figure error for optics in telescope. Milbond and EC2216 are the most popular epoxies that are used in optics. Bubbles usually exist in the epoxy when two components are mixed or translated to syringe. In this paper, the methods and experiments of adhesive mixture and injection in vacuum environment were explained to reduce the bonding surface figure of the mirror. The results show that adhesive mixture and injection in vacuum environment can dramatically decrease the bubble in the epoxy and greatly reduce the bonding surface figure error. </div>展开更多
1.Community of purpose makes friendship. 友谊来自共同的目标。2.Without confidence there is no friendship.友谊源于信任。3.Friendship’s the wine of life.友谊乃人生之美酒。4.Friendship multiplies joys,and divides griev...1.Community of purpose makes friendship. 友谊来自共同的目标。2.Without confidence there is no friendship.友谊源于信任。3.Friendship’s the wine of life.友谊乃人生之美酒。4.Friendship multiplies joys,and divides grieves.友谊使快乐倍增,使忧愁减半。5.Friendship is,at any rate,a relation of perfect equality.友谊是一种完全基于平等的关系。6.Fire is the test of gold,adversity of friendship.烈火试真金,患难见真情。7.A friend to everybody is a friend to nobody.滥交者无友。展开更多
基金Supported by The National Natural Science Foundation of China, No. 30972898
文摘AIM:To determine the expression of toll-like receptor 9(TLR9) in pancreatic tumor and the effects of cytosine phosphate-guanosine oligodeoxynucleotides 2216(CPG ODN2216) on biological behavior of pancreatic carcinoma cell line PANC-1 and explore their clinical significance.METHODS:The immunohistochemistry and Western blot were used to determine the expression of TLR9 protein in pancreatic cancer tissues,and immunofluorescence staining was performed to detect the TLR9 protein expression in pancreatic carcinoma cell line PANC-1.To assess the effects of CPG ODN2216 on the invasive property of Panc-1 cells,in vitro cell adhesion,wound-healing scrape,and invasion and cell colony formation were evaluated.RESULTS:TLR9 was highly expressed in pancreaticcancer tissues and PANC-1 cells.The percentage of positive cells expressing TLR9 protein in human pancreatic tissues,paracancerous tissues and normal tissues were 73.3%,33.3% and 20.0%,respectively,and the protein expression level of TLR9 was gradually descending(P < 0.05).In vitro tests in wound-healing scrape,cell adhesion,colony formation and matrigel invasion showed that the adhesion and motility of PANC-1 cells in CPG ODN 2216 treatment group were signif icantly lower than in the control group(P < 0.05).The cell growth assay showed that the proliferative ability of PANC-1 cells in treatment group was significantly decreased and CPG ODN2216 had an inhibitive effect in the growth of Panc-1 cells in a dose and time-dependent manner(P < 0.05).CONCLUSION:The gene of TLR9 is correlated with the invasive and metastatic potential of human pancreatic carcinoma,and CPG ODN2216 induces the inhibition of migration and invasion of Panc-1 cells.
文摘<div style="text-align:justify;"> Bonding is used widely when the optical glasses are connected with metal structures. It plays a very important role in the design of space telescope. Improper bonding may induce great surface figure error for optics in telescope. Milbond and EC2216 are the most popular epoxies that are used in optics. Bubbles usually exist in the epoxy when two components are mixed or translated to syringe. In this paper, the methods and experiments of adhesive mixture and injection in vacuum environment were explained to reduce the bonding surface figure of the mirror. The results show that adhesive mixture and injection in vacuum environment can dramatically decrease the bubble in the epoxy and greatly reduce the bonding surface figure error. </div>
文摘1.Community of purpose makes friendship. 友谊来自共同的目标。2.Without confidence there is no friendship.友谊源于信任。3.Friendship’s the wine of life.友谊乃人生之美酒。4.Friendship multiplies joys,and divides grieves.友谊使快乐倍增,使忧愁减半。5.Friendship is,at any rate,a relation of perfect equality.友谊是一种完全基于平等的关系。6.Fire is the test of gold,adversity of friendship.烈火试真金,患难见真情。7.A friend to everybody is a friend to nobody.滥交者无友。
