A magnetic-based geophysical study was performed across the southern part of Cameroon to investigate the boundary between the Archean Congo craton and the Pan-African metamorphic belt. Magnetic gradient techniques inc...A magnetic-based geophysical study was performed across the southern part of Cameroon to investigate the boundary between the Archean Congo craton and the Pan-African metamorphic belt. Magnetic gradient techniques including Euler deconvolution and Tilt derivative have been applied to an aeromagnetic data profile to determine the depth of sources and their lateral extension. 2.5D magnetic modeling shows that the prominent magnetic positive anomalies observed on total magnetic map of south Cameroon are produced by deep and strongly magnetic bodies under the Pan-African formations mainly an important dyke formation structure with a high susceptibility of 0.041 (SI units), at an average depth of 4148 m and with a lateral extension of about 10 km. These bodies are interpreted to have emplaced at high crustal levels in a continental collision zone and were subsequently metamorphosed at granulite grade conditions, during the Pan-African orogeny about 620 Ma ago.展开更多
This study is based on the analysis and interpretation of aeromagnetic data using version 8.4 of the Geosoft Oasis Montaj Software, to map the subsurface or deep geological structures that affected the geological form...This study is based on the analysis and interpretation of aeromagnetic data using version 8.4 of the Geosoft Oasis Montaj Software, to map the subsurface or deep geological structures that affected the geological formations of the Ngaoundere area. The use of the standard aeromagnetic methods made it possible to draw up the maps of the residual magnetic field reduced to the equator (RTE), the horizontal gradient (HG), the analytical signal (AS) and that of the Euler solutions (ED) to find the main magnetic facies corresponding to these structures. The geological formations of the studied area thus appear to be intensely fractured by a NE-SW (N45°E) and ENE-WSW (N70°E) main orientation fault system, the depth of which has been estimated by combining the three-analytical methods HG, AS and ED. Advanced magmatic map analysis revealed dikes associated with vertical faults in the studied area. The development of an interpretative geological map taking into account the basic geology, the deep faults, the identified dikes and the mineralization index made it possible to extract a correlation between geological structures and mineralization of the studied area. The 2.5D modelling of two magnetic profiles plotted on the reduced residual map at the equator was performed to approximate the geometry and depth of the dikes sector, which are potential sources of mineralization here.展开更多
Ground gravity survey was recently carried out in the Lolabe-Campo area, which constitutes the southern onshore portion of the Kribi-Campo sub-basin in South Cameroon. The obtained gravity data were processed and inte...Ground gravity survey was recently carried out in the Lolabe-Campo area, which constitutes the southern onshore portion of the Kribi-Campo sub-basin in South Cameroon. The obtained gravity data were processed and interpreted in order to elucidate the subsurface geology of the area in which preliminary studies highlighted the presence of an intrusive igneous body. In order to determine the nature and the characteristics of this body, spectral analysis, ideal body solution and 2.5D modelling methods were applied to the gravity data. The results suggest that the emplacement, breaking up and separation of the intrusive igneous body were the outcome of two tectonic events corresponding to an upward vertical translational tectonic movement followed by an anticlockwise rotational faulting tectonic movement. From a density contrast of 0.13 g·cmDž, the two isolated bodies have an estimated density of 2.80 g·cmDž which is comparatively higher than the average density of 2.67 g·cmDž of the autochthonous rocks. These two blocks are completely surrounded by both sedimentary and metamorphic formations and are confined between the depths of 0.6 km and 3.5 km. The highly dense nature of the rocks and their location around the Atlantic Ocean led to the conclusion that the two intrusive bodies are composed principally of gabbro. The disposition and shape of the open zone resulting from the separation of the blocks are suggestive of a trap for the accumulation of hydrocarbons and mineral resources.展开更多
We present a comprehensive description and benchmark evaluation of the global–regional chemical transport model called the Emission and Atmospheric Processes Integrated and Coupled Community(EPICC)model.The framework...We present a comprehensive description and benchmark evaluation of the global–regional chemical transport model called the Emission and Atmospheric Processes Integrated and Coupled Community(EPICC)model.The framework incorporates(1)grid configuration,(2)transport dynamics,(3)chemical mechanisms,(4)aerosol processes,(5)wet/dry deposition parameterizations,and(6)heterogeneous chemistry treatments associated with sulfate,nitrous acid(HONO)chemistry,and aerosol/cloud–photolysis interactions(APIs/CPIs).Openly shared with the atmospheric research community,the model facilitates integration of advanced physicochemical schemes to enhance simulation accuracy.Globally,the model demonstrates realistic representations of ozone(O_(3))and aerosol optical depth.The EPICC model generally demonstrates robust performance in simulating regional concentrations of O_(3) and PM_(2.5)(and its components)in China.It successfully captures vertical profiles of both global and regional O_(3).Notably,the model mitigates frequently reported sulfate underestimations in highly industrialized regions of China.The model accurately captures two regional severe pollution episodes observed in eastern China(January/June 2021).Sensitivity experiments highlight the critical roles of heterogeneous chemical mechanisms associated with sulfate,HONO chemistry,APIs,and CPIs in capturing PM_(2.5) and O_(3) concentrations in China.Improved sulfate mechanisms result in an increase of approximately 32.4%(2.8μg m^(−3))in simulated winter sulfate concentrations when observations exceed 10μg m^(−3).Enhanced HONO elevates winter O_(3) and PM_(2.5) by≤20 and≤10μg m^(−3),respectively.Overall,CPIs dominate over APIs in improving O_(3) and PM_(2.5) simulations across China.Locally,APIs mitigate PM_(2.5) and O_(3) discrepancies in the Sichuan Basin.Seasonal cloud–chemistry coupling explains the weaker impact of PM_(2.5) in summer.展开更多
With the increasing demand for understanding skin physiology and advancing regenerative medicine,in vitro three-dimensional(3D)functional skin tissue models have become vital tools in dermatological research.These mod...With the increasing demand for understanding skin physiology and advancing regenerative medicine,in vitro three-dimensional(3D)functional skin tissue models have become vital tools in dermatological research.These models effectively mimic the complex structure and functions of human skin.This review comprehensively discusses the latest advancements in construction techniques,material selection,and applications of 3D skin models.It highlights the advantages and challenges associated with cutting-edge technologies such as layer-by-layer cell coating,3D bioprinting,bio-spray technology,and photolithographic microfabrication in creating highly realistic skin models.Moreover,it examines the wide-ranging applications of 3D skin models,includingelucidation of skin disease mechanisms,investigation of skin barrier functions,studies on skin aging and repair,hair regeneration,efficacy screening of therapeutic agents,cosmetic safety assessment,and personalized medicine.Finally,this review anticipates future trends in developing 3D skin models with greater structural and functional complexity,enhanced multifunctionality,and improved clinical translation.展开更多
The intestine is a key component of the barrier,absorption,and immune systems,contributing significantly to maintaining internal homeostasis and influencing disease progression.Its distinctive physiological functions ...The intestine is a key component of the barrier,absorption,and immune systems,contributing significantly to maintaining internal homeostasis and influencing disease progression.Its distinctive physiological functions arise from a complex interplay between its structure and microenvironment.Recent advancements in bioengineering technologies now enable the construction of in vitro intestinal models that faithfully recapitulate the organizational and functional characteristics of native tissue.This review examines the interface between in vitro models and native intestinal biology,offering insights into the replication of organ functions from a manufacturing perspective.We explore bioengineering strategies that enable the mapping of cross-scale structures and the creation of biomimetic environments essential for physiological performance.Furthermore,we discuss pragmatic optimization strategies for applying these models to both physiological and pathological studies,thereby enhancing their translational potential for drug development,disease modeling,and personalized medicine.In contrast to previous reviews,this work proposes an engineering-centered framework for linking structural fabrication strategies to functional performance across intestinal model types.展开更多
Traumatic brain injury causes permanent cell death and can lead to long-term cognitive dysfunction,with no available treatments to repair the damaged brain tissue.Methods to track and understand traumatic brain injury...Traumatic brain injury causes permanent cell death and can lead to long-term cognitive dysfunction,with no available treatments to repair the damaged brain tissue.Methods to track and understand traumatic brain injury in humans are severely limited by the inaccessibility of living brain tissue,creating a need for in vitro model systems to study cellular mechanisms of degeneration and regeneration following injury.Here we describe methods to establish a 3D human brain tissue model,consisting of a silk-collagen composite scaffold seeded with human neurons,astrocytes,and microglia,to study neuro-regeneration after traumatic brain injury.Step-by-step fabrication,injury,and analytical assessments of the 3D“triculture”system are described.Using this tissue model system,we demonstrate that glial cells promote regeneration of neuronal networks within the injury site over several weeks post-injury.Further,we found that regenerating networks in the 3D triculture tissues did not secrete early markers of neurodegenerative disease,but displayed signs of excitatory/inhibitory imbalance,suggesting that pro-regenerative treatments for traumatic brain injury in the future may need to direct cell differentiation to promote proper function.The mechanical stability of this model system enables physiologically relevant impact injury and long-term culture capability,while its modular design enables modification of cell contents,extracellular matrix composition,and scaffold properties.This adaptability could allow the integration of patient-derived cells and genetic modifications to bridge research and clinical applications focused on personalized targeted therapies.This in vitro system provides a valuable platform for accelerating therapeutic advancements in traumatic brain injury and neurodegenerative disorders,ultimately improving patient outcomes.展开更多
The intracontinental subduction of a>200-km-long section of the Tajik-Tarim lithosphere beneath the Pamir Mountains is proposed to explain nearly 30 km of shortening in the Tajik fold-thrust belt and the Pamir upli...The intracontinental subduction of a>200-km-long section of the Tajik-Tarim lithosphere beneath the Pamir Mountains is proposed to explain nearly 30 km of shortening in the Tajik fold-thrust belt and the Pamir uplift.Seismic imaging revealed that the upper slab was scraped and that the lower slab had subducted to a depth of>150 km.These features constitute the tectonic complexity of the Pamirs,as well as the thermal subduction mechanism involved,which remains poorly understood.Hence,in this study,high-resolution three-dimensional(3D)kinematic modeling is applied to investigate the thermal structure and geometry of the subducting slab beneath the Pamirs.The modeled slab configuration reveals distinct along-strike variations,with a steeply dipping slab beneath the southern Pamirs,a more gently inclined slab beneath the northern Pamirs,and apparent upper slab termination at shallow depths beneath the Pamirs.The thermal field reveals a cold slab core after delamination,with temperatures ranging from 400℃to 800℃,enveloped by a hotter mantle reaching~1400℃.The occurrence of intermediate-depth earthquakes aligns primarily with colder slab regions,particularly near the slab tear-off below the southwestern Pamirs,indicating a strong correlation between slab temperature and seismicity.In contrast,the northern Pamirs exhibit reduced seismicity at depth,which is likely associated with thermal weakening and delamination.The central Pamirs show a significant thermal anomaly caused by a concave slab,where the coldest crust does not descend deeply,further suggesting crustal detachment or mechanical failure.The lateral asymmetry in slab temperature possibly explains the mechanism of lateral tearing and differential slab-mantle coupling.展开更多
Automation and intelligence have become the primary trends in the design of investment casting processes.However,the design of gating and riser systems still lacks precise quantitative evaluation criteria.Numerical si...Automation and intelligence have become the primary trends in the design of investment casting processes.However,the design of gating and riser systems still lacks precise quantitative evaluation criteria.Numerical simulation plays a significant role in quantitatively evaluating current processes and making targeted improvements,but its limitations lie in the inability to dynamically reflect the formation outcomes of castings under varying process conditions,making real-time adjustments to gating and riser designs challenging.In this study,an automated design model for gating and riser systems based on integrated parametric 3D modeling-simulation framework is proposed,which enhances the flexibility and usability of evaluating the casting process by simulation.Firstly,geometric feature extraction technology is employed to obtain the geometric information of the target casting.Based on this information,an automated design framework for gating and riser systems is established,incorporating multiple structural parameters for real-time process control.Subsequently,the simulation results for various structural parameters are analyzed,and the influence of these parameters on casting formation is thoroughly investigated.Finally,the optimal design scheme is generated and validated through experimental verification.Simulation analysis and experimental results show that using a larger gate neck(24 mm in side length) and external risers promotes a more uniform temperature distribution and a more stable flow state,effectively eliminating shrinkage cavities and enhancing process yield by 15%.展开更多
Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual pat...Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual patient responses towards different drugs.This study aimed to investigate the patient-derived breast cancer culture models for drug sensitivity evaluations.Methods:Tumor and adjacent tissues from female breast cancer patients were collected during surgery.Patient-derived breast cancer cells were cultured using the conditional reprogramming technique to establish 2D models.The obtained patient-derived conditional reprogramming breast cancer(CRBC)cells were subsequently embedded in alginate-gelatin methacryloyl hydrogel microspheres to form 3D culture models.Comparisons between 2D and 3D models were made using immunohistochemistry(tumor markers),MTS assays(cell viability),flow cytometry(apoptosis),transwell assays(migration),and Western blotting(protein expression).Drug sensitivity tests were conducted to evaluate patient-specific responses to anti-cancer agents.Results:2D and 3D culture models were successfully established using samples from eight patients.The 3D models retained histological and marker characteristics of the original tumors.Compared to 2D cultures,3D models exhibited increased apoptosis,enhanced drug resistance,elevated stem cell marker expression,and greater migration ability—features more reflective of in vivo tumor behavior.Conclusion:Patient-derived 3D CRBC models effectively mimic the in vivo tumor microenvironment and demonstrate stronger resistance to anti-cancer drugs than 2D models.These hydrogel-based models offer a cost-effective and clinically relevant platform for drug screening and personalized breast cancer treatment.展开更多
Human motion modeling is a core technology in computer animation,game development,and humancomputer interaction.In particular,generating natural and coherent in-between motion using only the initial and terminal frame...Human motion modeling is a core technology in computer animation,game development,and humancomputer interaction.In particular,generating natural and coherent in-between motion using only the initial and terminal frames remains a fundamental yet unresolved challenge.Existing methods typically rely on dense keyframe inputs or complex prior structures,making it difficult to balance motion quality and plausibility under conditions such as sparse constraints,long-term dependencies,and diverse motion styles.To address this,we propose a motion generation framework based on a frequency-domain diffusion model,which aims to better model complex motion distributions and enhance generation stability under sparse conditions.Our method maps motion sequences to the frequency domain via the Discrete Cosine Transform(DCT),enabling more effective modeling of low-frequency motion structures while suppressing high-frequency noise.A denoising network based on self-attention is introduced to capture long-range temporal dependencies and improve global structural awareness.Additionally,a multi-objective loss function is employed to jointly optimize motion smoothness,pose diversity,and anatomical consistency,enhancing the realism and physical plausibility of the generated sequences.Comparative experiments on the Human3.6M and LaFAN1 datasets demonstrate that our method outperforms state-of-the-art approaches across multiple performance metrics,showing stronger capabilities in generating intermediate motion frames.This research offers a new perspective and methodology for human motion generation and holds promise for applications in character animation,game development,and virtual interaction.展开更多
Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches ...Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches exhibit high error rates in borderline cases and necessitate expert manual review,which limits scalability and introduces inconsistencies between algorithmic outputs and expert judgment.To address these limitations,we propose a large vision-language model(VLM)designed as an“expert agent”to automatically refine similarity scores,ensuring alignment with expert decisions and overcoming key application bottlenecks.The model consists of two core components:(1)rule-based similarity calculation module generate initial spectral similarity scores,and(2)pre-trained large vision-language model fine-tuned via supervised learning and reinforcement learning with human feedback(RLHF)to emulate expert assessments.To facilitate training and evaluation,we introduce two expert-annotated datasets,Spec1k and SpecReason,which capture both quantitative corrections and qualitative reasoning patterns,allowing the model to emulate expert decision-making processes.Experimental results demonstrate that our method achieves 81.45%source attribution accuracy,38.24%higher than rule-based and machine learning baselines.Real-world deployment further validates its effectiveness.展开更多
Geophysical surveying is crucial in the investigation of mineral resources in poorly exposed areas such as SE-Cameroon, a region known for its gold mineral potential. In this paper, gravity survey is carried out in th...Geophysical surveying is crucial in the investigation of mineral resources in poorly exposed areas such as SE-Cameroon, a region known for its gold mineral potential. In this paper, gravity survey is carried out in the Batouri area, SE-Cameroon based on land gravity data from the Centre-south Cameroon. <span style="font-family:Verdana;">Therefore, an analytical polynomial separation program, based on least-square fi</span><span style="font-family:Verdana;">tting of a third-degree polynomial surface to the Bouguer anomaly map, was used to separate the regional/residual components in gravity data. This technique permitted to better understand the disposition of the deep and near surface structures responsible of the observed anomalies in the Batouri area. Spectral analysis and 2.5D modelling of two profiles P</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;"> (SW-NE) and P</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> (N-S) selected from the residual anomaly map provided depths to basement. These depths constrain the gravity models along the profiles, indicating a variable thickness of the sedimentary infill with an approximate anomaly of -</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">33 mGal. The 2.5D model of the basement shows a gravity body, with a signature suggesting two close and similar masses, which characterize the quartz-bearing formations associated here to granite and gneiss. Our work highlights a main heavy gravity: Gwé-Batouri anomaly, containing the major part of auriferous deposits located along the NE-SW direction. Further, three tectonic sub-basins bounded by normal faults have been highlighted at Guedal, Gwé, and Bélimban, in the south of Guedal-Bélimban depression. They are associated with the extension tectonics, more or less vertical tangential cuts and accidents that have affected the region. A correlation with previous results from tectonic, lithological and gold mineralization activities proves the relevance of the study and the need to intensify geophysical surveying in the area.</span></span></span>展开更多
Frequency-domain airborne electromagnetics is a proven geophysical exploration method.Presently,the interpretation is mainly based on resistivity-depth imaging and onedimensional layered inversion;nevertheless,it is d...Frequency-domain airborne electromagnetics is a proven geophysical exploration method.Presently,the interpretation is mainly based on resistivity-depth imaging and onedimensional layered inversion;nevertheless,it is difficult to obtain satisfactory results for two- or three-dimensional complex earth structures using 1D methods.3D forward modeling and inversion can be used but are hampered by computational limitations because of the large number of data.Thus,we developed a 2.5D frequency-domain airborne electromagnetic forward modeling and inversion algorithm.To eliminate the source singularities in the numerical simulations,we split the fields into primary and secondary fields.The primary fields are calculated using homogeneous or layered models with analytical solutions,and the secondary（scattered） fields are solved by the finite-element method.The linear system of equations is solved by using the large-scale sparse matrix parallel direct solver,which greatly improves the computational efficiency.The inversion algorithm was based on damping leastsquares and singular value decomposition and combined the pseudo forward modeling and reciprocity principle to compute the Jacobian matrix.Synthetic and field data were used to test the effectiveness of the proposed method.展开更多
As one of the new structural layout in the family of woven composites, 2.5D Woven Composites（2.5D-WC） have recently attracted an increasing interest owing to its excellent properties, i.e. high specific strength and...As one of the new structural layout in the family of woven composites, 2.5D Woven Composites（2.5D-WC） have recently attracted an increasing interest owing to its excellent properties, i.e. high specific strength and fatigue resistance, in the aerospace and automobile industry. Indepth understanding of the fatigue behavior of this material at un-ambient temperatures is critical for the engineering applications, especially in aero-engine field. Here, fatigue behavior of 2.5D-WC at different temperatures was numerically investigated based on the unit cell approach. Firstly, the unit cell model of 2.5D-WC was established using ANSYS software. Subsequently, the temperature-dependent fatigue life prediction model was built up. Finally, the fatigue lives alongside the damage evolution processes of 2.5D-WC at ambient temperature（20 ℃） and unambient temperature（180 ℃） were analyzed. The results show that numerical results are in good agreement with the relevant experimental results at 20 and 180 ℃. Fatigue behavior of 2.5D-WC is also sensitive to temperature, which is partially attributed to the mechanical properties of resin and the change of inclination angle of warp yarns. We hope that the proposed fatigue life prediction model and the findings could further promote the engineering application of 2.5D-WC, especially in aero-engine field.展开更多
文摘A magnetic-based geophysical study was performed across the southern part of Cameroon to investigate the boundary between the Archean Congo craton and the Pan-African metamorphic belt. Magnetic gradient techniques including Euler deconvolution and Tilt derivative have been applied to an aeromagnetic data profile to determine the depth of sources and their lateral extension. 2.5D magnetic modeling shows that the prominent magnetic positive anomalies observed on total magnetic map of south Cameroon are produced by deep and strongly magnetic bodies under the Pan-African formations mainly an important dyke formation structure with a high susceptibility of 0.041 (SI units), at an average depth of 4148 m and with a lateral extension of about 10 km. These bodies are interpreted to have emplaced at high crustal levels in a continental collision zone and were subsequently metamorphosed at granulite grade conditions, during the Pan-African orogeny about 620 Ma ago.
文摘This study is based on the analysis and interpretation of aeromagnetic data using version 8.4 of the Geosoft Oasis Montaj Software, to map the subsurface or deep geological structures that affected the geological formations of the Ngaoundere area. The use of the standard aeromagnetic methods made it possible to draw up the maps of the residual magnetic field reduced to the equator (RTE), the horizontal gradient (HG), the analytical signal (AS) and that of the Euler solutions (ED) to find the main magnetic facies corresponding to these structures. The geological formations of the studied area thus appear to be intensely fractured by a NE-SW (N45°E) and ENE-WSW (N70°E) main orientation fault system, the depth of which has been estimated by combining the three-analytical methods HG, AS and ED. Advanced magmatic map analysis revealed dikes associated with vertical faults in the studied area. The development of an interpretative geological map taking into account the basic geology, the deep faults, the identified dikes and the mineralization index made it possible to extract a correlation between geological structures and mineralization of the studied area. The 2.5D modelling of two magnetic profiles plotted on the reduced residual map at the equator was performed to approximate the geometry and depth of the dikes sector, which are potential sources of mineralization here.
文摘Ground gravity survey was recently carried out in the Lolabe-Campo area, which constitutes the southern onshore portion of the Kribi-Campo sub-basin in South Cameroon. The obtained gravity data were processed and interpreted in order to elucidate the subsurface geology of the area in which preliminary studies highlighted the presence of an intrusive igneous body. In order to determine the nature and the characteristics of this body, spectral analysis, ideal body solution and 2.5D modelling methods were applied to the gravity data. The results suggest that the emplacement, breaking up and separation of the intrusive igneous body were the outcome of two tectonic events corresponding to an upward vertical translational tectonic movement followed by an anticlockwise rotational faulting tectonic movement. From a density contrast of 0.13 g·cmDž, the two isolated bodies have an estimated density of 2.80 g·cmDž which is comparatively higher than the average density of 2.67 g·cmDž of the autochthonous rocks. These two blocks are completely surrounded by both sedimentary and metamorphic formations and are confined between the depths of 0.6 km and 3.5 km. The highly dense nature of the rocks and their location around the Atlantic Ocean led to the conclusion that the two intrusive bodies are composed principally of gabbro. The disposition and shape of the open zone resulting from the separation of the blocks are suggestive of a trap for the accumulation of hydrocarbons and mineral resources.
基金National Key Scientific and Technological Infrastructure project “Earth System Science Numerical Simulator Facility” (EarthLab)supported by the National Natural Science Foundation of China (Grant No. 92044302)the National Key Research Development Program of China (Grant No. 2022YFC3700703)
文摘We present a comprehensive description and benchmark evaluation of the global–regional chemical transport model called the Emission and Atmospheric Processes Integrated and Coupled Community(EPICC)model.The framework incorporates(1)grid configuration,(2)transport dynamics,(3)chemical mechanisms,(4)aerosol processes,(5)wet/dry deposition parameterizations,and(6)heterogeneous chemistry treatments associated with sulfate,nitrous acid(HONO)chemistry,and aerosol/cloud–photolysis interactions(APIs/CPIs).Openly shared with the atmospheric research community,the model facilitates integration of advanced physicochemical schemes to enhance simulation accuracy.Globally,the model demonstrates realistic representations of ozone(O_(3))and aerosol optical depth.The EPICC model generally demonstrates robust performance in simulating regional concentrations of O_(3) and PM_(2.5)(and its components)in China.It successfully captures vertical profiles of both global and regional O_(3).Notably,the model mitigates frequently reported sulfate underestimations in highly industrialized regions of China.The model accurately captures two regional severe pollution episodes observed in eastern China(January/June 2021).Sensitivity experiments highlight the critical roles of heterogeneous chemical mechanisms associated with sulfate,HONO chemistry,APIs,and CPIs in capturing PM_(2.5) and O_(3) concentrations in China.Improved sulfate mechanisms result in an increase of approximately 32.4%(2.8μg m^(−3))in simulated winter sulfate concentrations when observations exceed 10μg m^(−3).Enhanced HONO elevates winter O_(3) and PM_(2.5) by≤20 and≤10μg m^(−3),respectively.Overall,CPIs dominate over APIs in improving O_(3) and PM_(2.5) simulations across China.Locally,APIs mitigate PM_(2.5) and O_(3) discrepancies in the Sichuan Basin.Seasonal cloud–chemistry coupling explains the weaker impact of PM_(2.5) in summer.
文摘With the increasing demand for understanding skin physiology and advancing regenerative medicine,in vitro three-dimensional(3D)functional skin tissue models have become vital tools in dermatological research.These models effectively mimic the complex structure and functions of human skin.This review comprehensively discusses the latest advancements in construction techniques,material selection,and applications of 3D skin models.It highlights the advantages and challenges associated with cutting-edge technologies such as layer-by-layer cell coating,3D bioprinting,bio-spray technology,and photolithographic microfabrication in creating highly realistic skin models.Moreover,it examines the wide-ranging applications of 3D skin models,includingelucidation of skin disease mechanisms,investigation of skin barrier functions,studies on skin aging and repair,hair regeneration,efficacy screening of therapeutic agents,cosmetic safety assessment,and personalized medicine.Finally,this review anticipates future trends in developing 3D skin models with greater structural and functional complexity,enhanced multifunctionality,and improved clinical translation.
基金the support from the National Key Research and Development Program of China(Nos.2024YFB4607700 and 2018YFA0703000)the Natural Science Foundation of Zhejiang Province(Nos.LDQ23E050001 and LQ24H260006)+2 种基金the National Natural Science Foundation of China(Nos.62303290,52305325,and 52405305)Shanghai Magnolia Talent Program Pujiang Project(No.23PJD036)The project was also supported by the State Key Laboratory of Materials Processing and Die&Mould Technology,Huazhong University of Science and Technology(No.P2025-002).
文摘The intestine is a key component of the barrier,absorption,and immune systems,contributing significantly to maintaining internal homeostasis and influencing disease progression.Its distinctive physiological functions arise from a complex interplay between its structure and microenvironment.Recent advancements in bioengineering technologies now enable the construction of in vitro intestinal models that faithfully recapitulate the organizational and functional characteristics of native tissue.This review examines the interface between in vitro models and native intestinal biology,offering insights into the replication of organ functions from a manufacturing perspective.We explore bioengineering strategies that enable the mapping of cross-scale structures and the creation of biomimetic environments essential for physiological performance.Furthermore,we discuss pragmatic optimization strategies for applying these models to both physiological and pathological studies,thereby enhancing their translational potential for drug development,disease modeling,and personalized medicine.In contrast to previous reviews,this work proposes an engineering-centered framework for linking structural fabrication strategies to functional performance across intestinal model types.
基金supported by funding from the U.S.Department of Defense,Nos.W911NF-23-1-0276,W81XWH2211065the NIH,No.P41EB027062(all to DLK).
文摘Traumatic brain injury causes permanent cell death and can lead to long-term cognitive dysfunction,with no available treatments to repair the damaged brain tissue.Methods to track and understand traumatic brain injury in humans are severely limited by the inaccessibility of living brain tissue,creating a need for in vitro model systems to study cellular mechanisms of degeneration and regeneration following injury.Here we describe methods to establish a 3D human brain tissue model,consisting of a silk-collagen composite scaffold seeded with human neurons,astrocytes,and microglia,to study neuro-regeneration after traumatic brain injury.Step-by-step fabrication,injury,and analytical assessments of the 3D“triculture”system are described.Using this tissue model system,we demonstrate that glial cells promote regeneration of neuronal networks within the injury site over several weeks post-injury.Further,we found that regenerating networks in the 3D triculture tissues did not secrete early markers of neurodegenerative disease,but displayed signs of excitatory/inhibitory imbalance,suggesting that pro-regenerative treatments for traumatic brain injury in the future may need to direct cell differentiation to promote proper function.The mechanical stability of this model system enables physiologically relevant impact injury and long-term culture capability,while its modular design enables modification of cell contents,extracellular matrix composition,and scaffold properties.This adaptability could allow the integration of patient-derived cells and genetic modifications to bridge research and clinical applications focused on personalized targeted therapies.This in vitro system provides a valuable platform for accelerating therapeutic advancements in traumatic brain injury and neurodegenerative disorders,ultimately improving patient outcomes.
基金the Chinese Academy of Sciences Pioneer Hundred Talents Program and the Second Tibetan Plateau Scientific Expedition and Research Program(Grant No.2019QZKK0708)supported by a MEXT(Ministry of Education,Culture,Sports,Science and Technology)KAKENHI(Grants-in-Aid for Scientific Research)grant(Grant No.21H05203)Kobe University Strategic International Collaborative Research Grant(Type B Fostering Joint Research).
文摘The intracontinental subduction of a>200-km-long section of the Tajik-Tarim lithosphere beneath the Pamir Mountains is proposed to explain nearly 30 km of shortening in the Tajik fold-thrust belt and the Pamir uplift.Seismic imaging revealed that the upper slab was scraped and that the lower slab had subducted to a depth of>150 km.These features constitute the tectonic complexity of the Pamirs,as well as the thermal subduction mechanism involved,which remains poorly understood.Hence,in this study,high-resolution three-dimensional(3D)kinematic modeling is applied to investigate the thermal structure and geometry of the subducting slab beneath the Pamirs.The modeled slab configuration reveals distinct along-strike variations,with a steeply dipping slab beneath the southern Pamirs,a more gently inclined slab beneath the northern Pamirs,and apparent upper slab termination at shallow depths beneath the Pamirs.The thermal field reveals a cold slab core after delamination,with temperatures ranging from 400℃to 800℃,enveloped by a hotter mantle reaching~1400℃.The occurrence of intermediate-depth earthquakes aligns primarily with colder slab regions,particularly near the slab tear-off below the southwestern Pamirs,indicating a strong correlation between slab temperature and seismicity.In contrast,the northern Pamirs exhibit reduced seismicity at depth,which is likely associated with thermal weakening and delamination.The central Pamirs show a significant thermal anomaly caused by a concave slab,where the coldest crust does not descend deeply,further suggesting crustal detachment or mechanical failure.The lateral asymmetry in slab temperature possibly explains the mechanism of lateral tearing and differential slab-mantle coupling.
基金financially supported by the National Key Research and Development Program of China (2022YFB3706802)。
文摘Automation and intelligence have become the primary trends in the design of investment casting processes.However,the design of gating and riser systems still lacks precise quantitative evaluation criteria.Numerical simulation plays a significant role in quantitatively evaluating current processes and making targeted improvements,but its limitations lie in the inability to dynamically reflect the formation outcomes of castings under varying process conditions,making real-time adjustments to gating and riser designs challenging.In this study,an automated design model for gating and riser systems based on integrated parametric 3D modeling-simulation framework is proposed,which enhances the flexibility and usability of evaluating the casting process by simulation.Firstly,geometric feature extraction technology is employed to obtain the geometric information of the target casting.Based on this information,an automated design framework for gating and riser systems is established,incorporating multiple structural parameters for real-time process control.Subsequently,the simulation results for various structural parameters are analyzed,and the influence of these parameters on casting formation is thoroughly investigated.Finally,the optimal design scheme is generated and validated through experimental verification.Simulation analysis and experimental results show that using a larger gate neck(24 mm in side length) and external risers promotes a more uniform temperature distribution and a more stable flow state,effectively eliminating shrinkage cavities and enhancing process yield by 15%.
基金supported by the Natural Science Foundation of Guangdong Province(No.2021B1515120053)Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515140166).
文摘Background:Therapeutic responses of breast cancer vary among patients and lead to drug resistance and recurrence due to the heterogeneity.Current preclinical models,however,are inadequate for predicting individual patient responses towards different drugs.This study aimed to investigate the patient-derived breast cancer culture models for drug sensitivity evaluations.Methods:Tumor and adjacent tissues from female breast cancer patients were collected during surgery.Patient-derived breast cancer cells were cultured using the conditional reprogramming technique to establish 2D models.The obtained patient-derived conditional reprogramming breast cancer(CRBC)cells were subsequently embedded in alginate-gelatin methacryloyl hydrogel microspheres to form 3D culture models.Comparisons between 2D and 3D models were made using immunohistochemistry(tumor markers),MTS assays(cell viability),flow cytometry(apoptosis),transwell assays(migration),and Western blotting(protein expression).Drug sensitivity tests were conducted to evaluate patient-specific responses to anti-cancer agents.Results:2D and 3D culture models were successfully established using samples from eight patients.The 3D models retained histological and marker characteristics of the original tumors.Compared to 2D cultures,3D models exhibited increased apoptosis,enhanced drug resistance,elevated stem cell marker expression,and greater migration ability—features more reflective of in vivo tumor behavior.Conclusion:Patient-derived 3D CRBC models effectively mimic the in vivo tumor microenvironment and demonstrate stronger resistance to anti-cancer drugs than 2D models.These hydrogel-based models offer a cost-effective and clinically relevant platform for drug screening and personalized breast cancer treatment.
基金supported by the National Natural Science Foundation of China(Grant No.72161034).
文摘Human motion modeling is a core technology in computer animation,game development,and humancomputer interaction.In particular,generating natural and coherent in-between motion using only the initial and terminal frames remains a fundamental yet unresolved challenge.Existing methods typically rely on dense keyframe inputs or complex prior structures,making it difficult to balance motion quality and plausibility under conditions such as sparse constraints,long-term dependencies,and diverse motion styles.To address this,we propose a motion generation framework based on a frequency-domain diffusion model,which aims to better model complex motion distributions and enhance generation stability under sparse conditions.Our method maps motion sequences to the frequency domain via the Discrete Cosine Transform(DCT),enabling more effective modeling of low-frequency motion structures while suppressing high-frequency noise.A denoising network based on self-attention is introduced to capture long-range temporal dependencies and improve global structural awareness.Additionally,a multi-objective loss function is employed to jointly optimize motion smoothness,pose diversity,and anatomical consistency,enhancing the realism and physical plausibility of the generated sequences.Comparative experiments on the Human3.6M and LaFAN1 datasets demonstrate that our method outperforms state-of-the-art approaches across multiple performance metrics,showing stronger capabilities in generating intermediate motion frames.This research offers a new perspective and methodology for human motion generation and holds promise for applications in character animation,game development,and virtual interaction.
文摘Existing methods for tracing water pollution sources typically integrate three-dimensional excitationemission matrix(3D-EEM)fluorescence spectroscopy with similarity-based matching algorithms.However,these approaches exhibit high error rates in borderline cases and necessitate expert manual review,which limits scalability and introduces inconsistencies between algorithmic outputs and expert judgment.To address these limitations,we propose a large vision-language model(VLM)designed as an“expert agent”to automatically refine similarity scores,ensuring alignment with expert decisions and overcoming key application bottlenecks.The model consists of two core components:(1)rule-based similarity calculation module generate initial spectral similarity scores,and(2)pre-trained large vision-language model fine-tuned via supervised learning and reinforcement learning with human feedback(RLHF)to emulate expert assessments.To facilitate training and evaluation,we introduce two expert-annotated datasets,Spec1k and SpecReason,which capture both quantitative corrections and qualitative reasoning patterns,allowing the model to emulate expert decision-making processes.Experimental results demonstrate that our method achieves 81.45%source attribution accuracy,38.24%higher than rule-based and machine learning baselines.Real-world deployment further validates its effectiveness.
文摘Geophysical surveying is crucial in the investigation of mineral resources in poorly exposed areas such as SE-Cameroon, a region known for its gold mineral potential. In this paper, gravity survey is carried out in the Batouri area, SE-Cameroon based on land gravity data from the Centre-south Cameroon. <span style="font-family:Verdana;">Therefore, an analytical polynomial separation program, based on least-square fi</span><span style="font-family:Verdana;">tting of a third-degree polynomial surface to the Bouguer anomaly map, was used to separate the regional/residual components in gravity data. This technique permitted to better understand the disposition of the deep and near surface structures responsible of the observed anomalies in the Batouri area. Spectral analysis and 2.5D modelling of two profiles P</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;"> (SW-NE) and P</span><sub><span style="font-family:Verdana;">2</span></sub><span style="font-family:Verdana;"> (N-S) selected from the residual anomaly map provided depths to basement. These depths constrain the gravity models along the profiles, indicating a variable thickness of the sedimentary infill with an approximate anomaly of -</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">33 mGal. The 2.5D model of the basement shows a gravity body, with a signature suggesting two close and similar masses, which characterize the quartz-bearing formations associated here to granite and gneiss. Our work highlights a main heavy gravity: Gwé-Batouri anomaly, containing the major part of auriferous deposits located along the NE-SW direction. Further, three tectonic sub-basins bounded by normal faults have been highlighted at Guedal, Gwé, and Bélimban, in the south of Guedal-Bélimban depression. They are associated with the extension tectonics, more or less vertical tangential cuts and accidents that have affected the region. A correlation with previous results from tectonic, lithological and gold mineralization activities proves the relevance of the study and the need to intensify geophysical surveying in the area.</span></span></span>
基金supported by the Doctoral Fund Project of the Ministry of Education(No.20130061110060 class tutors)the National Natural Science Foundation of China(No.41504083)National Basic Research Program of China(973Program)(No.2013CB429805)
文摘Frequency-domain airborne electromagnetics is a proven geophysical exploration method.Presently,the interpretation is mainly based on resistivity-depth imaging and onedimensional layered inversion;nevertheless,it is difficult to obtain satisfactory results for two- or three-dimensional complex earth structures using 1D methods.3D forward modeling and inversion can be used but are hampered by computational limitations because of the large number of data.Thus,we developed a 2.5D frequency-domain airborne electromagnetic forward modeling and inversion algorithm.To eliminate the source singularities in the numerical simulations,we split the fields into primary and secondary fields.The primary fields are calculated using homogeneous or layered models with analytical solutions,and the secondary（scattered） fields are solved by the finite-element method.The linear system of equations is solved by using the large-scale sparse matrix parallel direct solver,which greatly improves the computational efficiency.The inversion algorithm was based on damping leastsquares and singular value decomposition and combined the pseudo forward modeling and reciprocity principle to compute the Jacobian matrix.Synthetic and field data were used to test the effectiveness of the proposed method.
基金supported by Jiangsu Innovation Program fo Graduate Education (No. KYLX_0237)
文摘As one of the new structural layout in the family of woven composites, 2.5D Woven Composites（2.5D-WC） have recently attracted an increasing interest owing to its excellent properties, i.e. high specific strength and fatigue resistance, in the aerospace and automobile industry. Indepth understanding of the fatigue behavior of this material at un-ambient temperatures is critical for the engineering applications, especially in aero-engine field. Here, fatigue behavior of 2.5D-WC at different temperatures was numerically investigated based on the unit cell approach. Firstly, the unit cell model of 2.5D-WC was established using ANSYS software. Subsequently, the temperature-dependent fatigue life prediction model was built up. Finally, the fatigue lives alongside the damage evolution processes of 2.5D-WC at ambient temperature（20 ℃） and unambient temperature（180 ℃） were analyzed. The results show that numerical results are in good agreement with the relevant experimental results at 20 and 180 ℃. Fatigue behavior of 2.5D-WC is also sensitive to temperature, which is partially attributed to the mechanical properties of resin and the change of inclination angle of warp yarns. We hope that the proposed fatigue life prediction model and the findings could further promote the engineering application of 2.5D-WC, especially in aero-engine field.
