The lipases from different sources were screened for their ability to catalyze the resolution of 2-octanol in organic solvents with vinyl acetate as the acylating reagent. The medium effect has been studied on the irr...The lipases from different sources were screened for their ability to catalyze the resolution of 2-octanol in organic solvents with vinyl acetate as the acylating reagent. The medium effect has been studied on the irreversible transesterification with varying water activity(a_w). The influence of vinyl acetate concentration on it has also been investigated. Under the optimal conditions, the enantiomeric ratio(E value) of pseudomonas fluorescence lipase(PFL) exceeded 200 with an enantiomeric excess(e.e.) of S-2-octanol above 99% at a 51% degree of conversion.展开更多
A new method is proposed to absorb the NOx into an organic compound,2-octanol. The absorption efficiency reaches up to 96-99%,and the NOx emission level is decreased from 1.0×10~4-3.0×10~5 to 60-680Ppm.In th...A new method is proposed to absorb the NOx into an organic compound,2-octanol. The absorption efficiency reaches up to 96-99%,and the NOx emission level is decreased from 1.0×10~4-3.0×10~5 to 60-680Ppm.In the absorption process,2-octanol is preoxidized.And then it is further oxidized into hexanoic acid with nitric acid.In this way,20% of nitric acid is saved for the same yield of hexanoic acid.展开更多
Chiral 2-octanol is one of the key intermediates for preparation of liquid crystal materials, as well as many optically active pharmaceuticals. Lipase catalyzed kinetic resolution has proved to be an efficient techniq...Chiral 2-octanol is one of the key intermediates for preparation of liquid crystal materials, as well as many optically active pharmaceuticals. Lipase catalyzed kinetic resolution has proved to be an efficient technique for synthesis of enantiomerically enriched compounds. In the present study, optimization and kinetic modeling of kinetic resolution of (±)-2-octanol was done by using vinyl acetate as an acyl donor in n-heptane as a solvent. Response surface methodology (RSM) and four-factor-five-level Centre Composite Rotatable Design (CCRD) were employed to evaluate the effect of various parameters such as speed of agitation, enzyme loading, temperature and acyl donor/alcohol molar ratio on conversion, enantiomeric excess (ee), enantioselectivity and initial rate of reaction. Acylation of 2-octanol with vinyl acetate catalyzed by Novozyme 435 follows the ternary complex mechanism (ordered bi-bi mechanism) with inhibition by 2-octanol.展开更多
基金Supported by the National Natural Science Foundation of China(No.2 0 0 72 0 11and2 0 2 72 0 17)
文摘The lipases from different sources were screened for their ability to catalyze the resolution of 2-octanol in organic solvents with vinyl acetate as the acylating reagent. The medium effect has been studied on the irreversible transesterification with varying water activity(a_w). The influence of vinyl acetate concentration on it has also been investigated. Under the optimal conditions, the enantiomeric ratio(E value) of pseudomonas fluorescence lipase(PFL) exceeded 200 with an enantiomeric excess(e.e.) of S-2-octanol above 99% at a 51% degree of conversion.
文摘A new method is proposed to absorb the NOx into an organic compound,2-octanol. The absorption efficiency reaches up to 96-99%,and the NOx emission level is decreased from 1.0×10~4-3.0×10~5 to 60-680Ppm.In the absorption process,2-octanol is preoxidized.And then it is further oxidized into hexanoic acid with nitric acid.In this way,20% of nitric acid is saved for the same yield of hexanoic acid.
文摘Chiral 2-octanol is one of the key intermediates for preparation of liquid crystal materials, as well as many optically active pharmaceuticals. Lipase catalyzed kinetic resolution has proved to be an efficient technique for synthesis of enantiomerically enriched compounds. In the present study, optimization and kinetic modeling of kinetic resolution of (±)-2-octanol was done by using vinyl acetate as an acyl donor in n-heptane as a solvent. Response surface methodology (RSM) and four-factor-five-level Centre Composite Rotatable Design (CCRD) were employed to evaluate the effect of various parameters such as speed of agitation, enzyme loading, temperature and acyl donor/alcohol molar ratio on conversion, enantiomeric excess (ee), enantioselectivity and initial rate of reaction. Acylation of 2-octanol with vinyl acetate catalyzed by Novozyme 435 follows the ternary complex mechanism (ordered bi-bi mechanism) with inhibition by 2-octanol.
