Metal (Me=Fe(III), Mo(VI), Mn(II), Co(II), Ni(II), Zn(II) and Cu(II)) 2-hydroxy-1-naphthaldehyde thiosemicarbazone complexes (MeHNT) were synthesized and used as mimic-enzyme catalysts to mimic the active group of hor...Metal (Me=Fe(III), Mo(VI), Mn(II), Co(II), Ni(II), Zn(II) and Cu(II)) 2-hydroxy-1-naphthaldehyde thiosemicarbazone complexes (MeHNT) were synthesized and used as mimic-enzyme catalysts to mimic the active group of horseradish peroxidase (HRP). The results showed that Fe-HNT, Mo-HNT are effective catalysts, which have similar catalytic activity as HRP. The sequence of catalytic activities of tested biomimic peroxidas is Mo-HNT > Fe-HNT > Zn-HNT > Ni-HNT > Mn-HNT. Among them, Fe-HNT is used as a mimic-enzyme catalyst in determination of ascorbic acid and glucose by coupling the catalytic reaction of glucose oxidase.展开更多
In an alkaline 2-propanol solution with 5,10,15,20-tetra(4-methoxyl phenyl) porphyrin iron chloride(TOMPPFeCl) as a catalyst and oxygen as a cheap green oxidant, 2-naphthol was conversed to 2-hydroxy-\{1,4-naphthoquin...In an alkaline 2-propanol solution with 5,10,15,20-tetra(4-methoxyl phenyl) porphyrin iron chloride(TOMPPFeCl) as a catalyst and oxygen as a cheap green oxidant, 2-naphthol was conversed to 2-hydroxy-\{1,4-naphthoquinone(HNQ)\} with a yield of 62.17% and a selectivity of 100%, and the conversion number of TMOPPFeCl catalyst was 8.32/min. The catalytic oxidation products were characterized by means of UV-Vis, IR, GC-MS, ~ 1H NMR and melting point determination. In this catalytic oxidation, the catalytic activity of TMOPPFeCl was researched in detail and the reacting conditions were optimized. A possible reaction mechanism is summarized based on in situ EPR determination.展开更多
The interaction of N-(2-methyl thiophenyl)-2-hydroxy-1-naphthaldimine (NMTHN) with tin dioxide nanoparticles (SnO2 NPs) has been investigated by spectroscopic tools such as absorption and fluorescence spectroscopy. Ab...The interaction of N-(2-methyl thiophenyl)-2-hydroxy-1-naphthaldimine (NMTHN) with tin dioxide nanoparticles (SnO2 NPs) has been investigated by spectroscopic tools such as absorption and fluorescence spectroscopy. Absorption spectroscopy reveals the formation of ground state complex. Fluorescence spectroscopy has been used to study the signatures of fluorescence quenching. SnO2 NPs are found to quench the intrinsic fluorescence of NMTHN via static and dynamic quenching. The deviation from linearity in the Stern-Volmer plot has been observed.展开更多
A mononuclear copper(II) complex, [Cu(bipy)(naph)(ClO4)] (where bipy is bipyridine and naph is 2-hydroxy-1-naphthaldehyde), was synthesized and characterized by X-ray single-crystal structure analysis. The crystal is ...A mononuclear copper(II) complex, [Cu(bipy)(naph)(ClO4)] (where bipy is bipyridine and naph is 2-hydroxy-1-naphthaldehyde), was synthesized and characterized by X-ray single-crystal structure analysis. The crystal is triclinic, space group P ?with a = 9.245(4), b = 9.962(4), c = 10.809(7) ? a = 84.83(5), b =82.35(4), g = 81.02(4), V = 972.1 ?, C21H15ClCuN2O6 Mr = 490.36, Z = 2, F(000) = 498, Dx = 1.68 g/cm3, m = 13.05 cm-1, R = 0.078, Rw = 0.081 for 2295 observed reflections with I > 3s(I). The copper(II) ion is coordinated by two nitrogen atoms of bipy and two oxygen atoms of naph in the equatorial plane, with an axial perchlorate oxygen-copper(II) bond to copper(II) ion to form square-pyramidal coordination geometry. The coordination environment of copper(II) is similar to the active site of galactose oxidase and this compound may also be considered as the structural model of galactose oxidase.展开更多
目的探究阿尔茨海默病患者血清视锥蛋白样蛋白1(visinin-like protein,VILIP-1)、帕金森病蛋白2(Parkinson disease protein 2,PARK2)的表达及临床意义。方法选取2021年5月至2024年5月收治的96例阿尔兹海默病患者作为病例组,根据病情严...目的探究阿尔茨海默病患者血清视锥蛋白样蛋白1(visinin-like protein,VILIP-1)、帕金森病蛋白2(Parkinson disease protein 2,PARK2)的表达及临床意义。方法选取2021年5月至2024年5月收治的96例阿尔兹海默病患者作为病例组,根据病情严重程度进一步分为轻度组43例,中度组31例,重度组22例;另选择96例体检健康志愿者作为对照组。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)对所有血清样本VILIP-1、PARK2表达水平进行检测;用Pearson相关分析阿尔茨海默病患者血清VILIP-1、PARK2表达水平与简易智力状态评分(Mini Mental State Examination,MMSE)评分及炎性因子的相关性;受试者工作特征(receiver operating characteristic,ROC)曲线,分析血清VILIP-1、PARK2水平对阿尔兹海默病的诊断价值。结果与对照组比较,病例组患者的血清VILIP-1及炎性因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-17(interleukin-17,IL-17)表达水平均升高,PARK2水平及MMSE评分均降低(P<0.05);随着阿尔茨海默病病情进展,血清VILIP-1表达水平逐渐升高,PARK2表达水平逐渐降低(P<0.05);Pearson相关分析结果显示阿尔茨海默病患者血清VILIP-1与MMSE评分呈负相关,与炎性因子TNF-α、IL-1β、IL-6、IL-17呈正相关(P<0.05);血清PARK2表达水平与MMSE评分呈正相关,与TNF-α、IL-1β、IL-6、IL-17呈负相关(P<0.05);ROC曲线结果表明,血清VILIP-1、PARK2联合诊断阿尔兹海默病患病的曲线下面积(area under the curve,AUC)值为0.883,显著大于VILIP-1(Z=2.531,P=0.011)和PARK2(Z=2.332,P=0.020)单独诊断。结论阿尔茨海默病患者血清VILIP-1表达水平升高,PARK2表达水平降低,二者与病情严重程度有关,同时检测两者的水平对阿尔茨海默病的诊断具有较高价值。展开更多
目的:探索通用转录因子ⅡE肽1(GTF2E1)在结直肠癌(CRC)中的表达水平及其对肿瘤细胞增殖的影响,以评估其作为潜在生物标志物和治疗靶点的价值。方法:通过收集并分析来自公共数据库的CRC样本数据,结合临床样本的免疫组化结果,利用单细胞...目的:探索通用转录因子ⅡE肽1(GTF2E1)在结直肠癌(CRC)中的表达水平及其对肿瘤细胞增殖的影响,以评估其作为潜在生物标志物和治疗靶点的价值。方法:通过收集并分析来自公共数据库的CRC样本数据,结合临床样本的免疫组化结果,利用单细胞测序技术和成簇规律间隔短回文重复序列(CRISPR)敲除实验,全面评价GTF2E1在CRC中的表达模式和作用。结果:GTF2E1在CRC组织中呈显著高表达,公共数据集、单细胞分析及内部临床样本检测结果一致。其诊断CRC的综合受试者工作特征曲线下面积为0.87,具有良好的诊断效能;CRISPR敲除实验显示,GTF 2 E 1敲除可显著抑制61种CRC细胞系的增殖。结论:GTF2E1在CRC的发生与增殖中起重要作用,有潜力成为CRC的生物标志物和治疗靶点。展开更多
文摘Metal (Me=Fe(III), Mo(VI), Mn(II), Co(II), Ni(II), Zn(II) and Cu(II)) 2-hydroxy-1-naphthaldehyde thiosemicarbazone complexes (MeHNT) were synthesized and used as mimic-enzyme catalysts to mimic the active group of horseradish peroxidase (HRP). The results showed that Fe-HNT, Mo-HNT are effective catalysts, which have similar catalytic activity as HRP. The sequence of catalytic activities of tested biomimic peroxidas is Mo-HNT > Fe-HNT > Zn-HNT > Ni-HNT > Mn-HNT. Among them, Fe-HNT is used as a mimic-enzyme catalyst in determination of ascorbic acid and glucose by coupling the catalytic reaction of glucose oxidase.
文摘In an alkaline 2-propanol solution with 5,10,15,20-tetra(4-methoxyl phenyl) porphyrin iron chloride(TOMPPFeCl) as a catalyst and oxygen as a cheap green oxidant, 2-naphthol was conversed to 2-hydroxy-\{1,4-naphthoquinone(HNQ)\} with a yield of 62.17% and a selectivity of 100%, and the conversion number of TMOPPFeCl catalyst was 8.32/min. The catalytic oxidation products were characterized by means of UV-Vis, IR, GC-MS, ~ 1H NMR and melting point determination. In this catalytic oxidation, the catalytic activity of TMOPPFeCl was researched in detail and the reacting conditions were optimized. A possible reaction mechanism is summarized based on in situ EPR determination.
文摘The interaction of N-(2-methyl thiophenyl)-2-hydroxy-1-naphthaldimine (NMTHN) with tin dioxide nanoparticles (SnO2 NPs) has been investigated by spectroscopic tools such as absorption and fluorescence spectroscopy. Absorption spectroscopy reveals the formation of ground state complex. Fluorescence spectroscopy has been used to study the signatures of fluorescence quenching. SnO2 NPs are found to quench the intrinsic fluorescence of NMTHN via static and dynamic quenching. The deviation from linearity in the Stern-Volmer plot has been observed.
基金Supported by the National Natural Science Foundation of China (No. 29971017) the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE China.
文摘A mononuclear copper(II) complex, [Cu(bipy)(naph)(ClO4)] (where bipy is bipyridine and naph is 2-hydroxy-1-naphthaldehyde), was synthesized and characterized by X-ray single-crystal structure analysis. The crystal is triclinic, space group P ?with a = 9.245(4), b = 9.962(4), c = 10.809(7) ? a = 84.83(5), b =82.35(4), g = 81.02(4), V = 972.1 ?, C21H15ClCuN2O6 Mr = 490.36, Z = 2, F(000) = 498, Dx = 1.68 g/cm3, m = 13.05 cm-1, R = 0.078, Rw = 0.081 for 2295 observed reflections with I > 3s(I). The copper(II) ion is coordinated by two nitrogen atoms of bipy and two oxygen atoms of naph in the equatorial plane, with an axial perchlorate oxygen-copper(II) bond to copper(II) ion to form square-pyramidal coordination geometry. The coordination environment of copper(II) is similar to the active site of galactose oxidase and this compound may also be considered as the structural model of galactose oxidase.
文摘目的探究阿尔茨海默病患者血清视锥蛋白样蛋白1(visinin-like protein,VILIP-1)、帕金森病蛋白2(Parkinson disease protein 2,PARK2)的表达及临床意义。方法选取2021年5月至2024年5月收治的96例阿尔兹海默病患者作为病例组,根据病情严重程度进一步分为轻度组43例,中度组31例,重度组22例;另选择96例体检健康志愿者作为对照组。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)对所有血清样本VILIP-1、PARK2表达水平进行检测;用Pearson相关分析阿尔茨海默病患者血清VILIP-1、PARK2表达水平与简易智力状态评分(Mini Mental State Examination,MMSE)评分及炎性因子的相关性;受试者工作特征(receiver operating characteristic,ROC)曲线,分析血清VILIP-1、PARK2水平对阿尔兹海默病的诊断价值。结果与对照组比较,病例组患者的血清VILIP-1及炎性因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-6(interleukin-6,IL-6)、白细胞介素-17(interleukin-17,IL-17)表达水平均升高,PARK2水平及MMSE评分均降低(P<0.05);随着阿尔茨海默病病情进展,血清VILIP-1表达水平逐渐升高,PARK2表达水平逐渐降低(P<0.05);Pearson相关分析结果显示阿尔茨海默病患者血清VILIP-1与MMSE评分呈负相关,与炎性因子TNF-α、IL-1β、IL-6、IL-17呈正相关(P<0.05);血清PARK2表达水平与MMSE评分呈正相关,与TNF-α、IL-1β、IL-6、IL-17呈负相关(P<0.05);ROC曲线结果表明,血清VILIP-1、PARK2联合诊断阿尔兹海默病患病的曲线下面积(area under the curve,AUC)值为0.883,显著大于VILIP-1(Z=2.531,P=0.011)和PARK2(Z=2.332,P=0.020)单独诊断。结论阿尔茨海默病患者血清VILIP-1表达水平升高,PARK2表达水平降低,二者与病情严重程度有关,同时检测两者的水平对阿尔茨海默病的诊断具有较高价值。
基金江西省自然科学基金项目(编号:20202BAB206075)江西省教育厅科技项目(编号:GJJ201202)江西中医药大学中西医结合一级学科平台(Discipline of Chinese and Western Integrative Medicine,Jiangxi University of Chinese Medicine)。
文摘目的:探索通用转录因子ⅡE肽1(GTF2E1)在结直肠癌(CRC)中的表达水平及其对肿瘤细胞增殖的影响,以评估其作为潜在生物标志物和治疗靶点的价值。方法:通过收集并分析来自公共数据库的CRC样本数据,结合临床样本的免疫组化结果,利用单细胞测序技术和成簇规律间隔短回文重复序列(CRISPR)敲除实验,全面评价GTF2E1在CRC中的表达模式和作用。结果:GTF2E1在CRC组织中呈显著高表达,公共数据集、单细胞分析及内部临床样本检测结果一致。其诊断CRC的综合受试者工作特征曲线下面积为0.87,具有良好的诊断效能;CRISPR敲除实验显示,GTF 2 E 1敲除可显著抑制61种CRC细胞系的增殖。结论:GTF2E1在CRC的发生与增殖中起重要作用,有潜力成为CRC的生物标志物和治疗靶点。
