This letter presents a method for digital image watermarking for copyright protection. This technique produces a watermarked image that closely retains the quality of the original host image while concurrently survivi...This letter presents a method for digital image watermarking for copyright protection. This technique produces a watermarked image that closely retains the quality of the original host image while concurrently surviving various image processing operations such as lowpass/highpass filtering, lossy JPEG compression, and cropping. This image watermarking algorithm takes full advantage of permutation and 2-D barcode (PDF417). The actual watermark embedding in spatial domain is followed using permutated image for improving the resistance to image cropping. Much higher watermark robustness is obtainable via a simple forward error correction technique, which is the main feature of PDF417 codes. Additional features of this technique include the easy determination of the existence of the watermark and that the watermark verification procedure does not need the original host image. The experimental results demonstrate its effectiveness.展开更多
To ensure the safety of medications,it is vital to accurately authenticate species of the Apocynaceae family,which is rich in poisonous medicinal plants.We identified Apocynaceae species by using nuclear internal tran...To ensure the safety of medications,it is vital to accurately authenticate species of the Apocynaceae family,which is rich in poisonous medicinal plants.We identified Apocynaceae species by using nuclear internal transcribed spacer 2(ITS2)and psb Atrn H based on experimental data.The identification ability of ITS2 and psb A-trn H was assessed using specific genetic divergence,BLAST1,and neighbor-joining trees.For DNA barcoding,ITS2 and psb A-trn H regions of 122 plant samples of 31 species from 19 genera in the Apocynaceae family were amplified.The PCR amplification for ITS2 and psb A-trn H sequences was 100%.The sequencing success rates for ITS2 and psb A-trn H sequences were 81%and 61%,respectively.Additional data involved 53 sequences of the ITS2 region and 38 sequences of the psb A-trn H region were downloaded from Gen Bank.Moreover,the analysis showed that the interspecific divergence of Apocynaceae species was greater than its intra-specific variations.The results indicated that,using the BLAST1 method,ITS2 showed a high identification efficiency of 97%and 100%of the samples at the species and genus levels,respectively,via BLAST1,and psb A-trn H successfully identified 95%and 100%of the samples at the species and genus levels,respectively.The barcode combination of ITS2/psb A-trn H successfully identified 98%and 100%of samples at the species and genus levels,respectively.Subsequently,the neighbor joining tree method also showed that barcode ITS2 and psb A-trn H could distinguish among the species within the Apocynaceae family.ITS2 is a core barcode and psb A-trn H is a supplementary barcode for identifying species in the Apocynaceae family.These results will help to improve DNA barcoding reference databases for herbal drugs and other herbal raw materials.展开更多
Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has...Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective.展开更多
In this paper,we consider the initial boundary value problem for the 2-D hyperbolic viscous Cahn-Hilliard equation.Firstly,we prove the existence and uniqueness of the local solution by the Galerkin method and contrac...In this paper,we consider the initial boundary value problem for the 2-D hyperbolic viscous Cahn-Hilliard equation.Firstly,we prove the existence and uniqueness of the local solution by the Galerkin method and contraction mapping principle.Then,using the potential well theory,we study the global well-posedness of the solution with initial data at different levels of initial energy,i.e.,subcritical initial energy,critical initial energy and arbitrary positive initial energy.For subcritical initial energy,we prove the global existence,asymptotic behavior and finite time blowup of the solution.Moreover,we extend these results to the critical initial energy using the scaling technique.For arbitrary positive initial energy,including the sup-critical initial energy,we obtain the sufficient conditions for finite time blow-up of the solution.As a further study for estimating the blowup time,we give a unified expression of the lower bound of blowup time for all three initial energy levels and estimate the upper bound of blowup time for subcritical and critical initial energy.展开更多
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the pathogen responsible for coronavirus disease 2019(COVID-19),continues to evolve,giving rise to more variants and global reinfections.Previous research ha...Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the pathogen responsible for coronavirus disease 2019(COVID-19),continues to evolve,giving rise to more variants and global reinfections.Previous research has demonstrated that barcode segments can effectively and cost-efficiently identify specific species within closely related populations.In this study,we designed and tested RNA barcode segments based on genetic evolutionary relationships to facilitate the efficient and accurate identification of SARS-CoV-2 from extensive virus samples,including human coronaviruses(HCoVs)and SARSr-CoV-2 lineages.Nucleotide sequences sourced from NCBI and GISAID were meticulously selected and curated to construct training sets,encompassing 1733 complete genome sequences of HCoVs and SARSr-CoV-2 lineages.Through genetic-level species testing,we validated the accuracy and reliability of the barcode segments for identifying SARS-CoV-2.Subsequently,75 main and subordinate species-specific barcode segments for SARS-CoV-2,located in ORF1ab,S,E,ORF7a,and N coding sequences,were intercepted and screened based on single-nucleotide polymorphism sites and weighted scores.Post-testing,these segments exhibited high recall rates(nearly 100%),specificity(almost 30%at the nucleotide level),and precision(100%)performance on identification.They were eventually visualized using one and two-dimensional combined barcodes and deposited in an online database(http://virusbarcodedatabase.top/).The successful integration of barcoding technology in SARS-CoV-2 identification provides valuable insights for future studies involving complete genome sequence polymorphism analysis.Moreover,this cost-effective and efficient identification approach also provides valuable reference for future research endeavors related to virus surveillance.展开更多
文摘This letter presents a method for digital image watermarking for copyright protection. This technique produces a watermarked image that closely retains the quality of the original host image while concurrently surviving various image processing operations such as lowpass/highpass filtering, lossy JPEG compression, and cropping. This image watermarking algorithm takes full advantage of permutation and 2-D barcode (PDF417). The actual watermark embedding in spatial domain is followed using permutated image for improving the resistance to image cropping. Much higher watermark robustness is obtainable via a simple forward error correction technique, which is the main feature of PDF417 codes. Additional features of this technique include the easy determination of the existence of the watermark and that the watermark verification procedure does not need the original host image. The experimental results demonstrate its effectiveness.
基金supported by the CAMS Initiative for Innovative Medicine(No.2016-12M-2-003)the Special Subsidies for Public Health Services of TCM("The National Survey of TCM Resources",DSS,MOF,No.66/2017)。
文摘To ensure the safety of medications,it is vital to accurately authenticate species of the Apocynaceae family,which is rich in poisonous medicinal plants.We identified Apocynaceae species by using nuclear internal transcribed spacer 2(ITS2)and psb Atrn H based on experimental data.The identification ability of ITS2 and psb A-trn H was assessed using specific genetic divergence,BLAST1,and neighbor-joining trees.For DNA barcoding,ITS2 and psb A-trn H regions of 122 plant samples of 31 species from 19 genera in the Apocynaceae family were amplified.The PCR amplification for ITS2 and psb A-trn H sequences was 100%.The sequencing success rates for ITS2 and psb A-trn H sequences were 81%and 61%,respectively.Additional data involved 53 sequences of the ITS2 region and 38 sequences of the psb A-trn H region were downloaded from Gen Bank.Moreover,the analysis showed that the interspecific divergence of Apocynaceae species was greater than its intra-specific variations.The results indicated that,using the BLAST1 method,ITS2 showed a high identification efficiency of 97%and 100%of the samples at the species and genus levels,respectively,via BLAST1,and psb A-trn H successfully identified 95%and 100%of the samples at the species and genus levels,respectively.The barcode combination of ITS2/psb A-trn H successfully identified 98%and 100%of samples at the species and genus levels,respectively.Subsequently,the neighbor joining tree method also showed that barcode ITS2 and psb A-trn H could distinguish among the species within the Apocynaceae family.ITS2 is a core barcode and psb A-trn H is a supplementary barcode for identifying species in the Apocynaceae family.These results will help to improve DNA barcoding reference databases for herbal drugs and other herbal raw materials.
基金Supported by National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2012BAD35B04)
文摘Two-dimensional(2D) barcode technology is an electronic tagging technology based on combination of computer and optical technology. It is an important way of information collection and input. 2D barcode technology has been widely used in various fields of logistics,production automation,and e-commerce,but it also has brought about a series of safety problems. Based on evolutionary encryption technology,this paper improved algorithm of traditional 2D barcode generation,to improve forgery- proof performance of 2D barcode. This algorithm is applied to agricultural products quality and safety traceability system and the results show that it is effective.
基金supported by the NSFC(12271122)the Fundamental Research Funds for the Central Universities.Han’s research was supported by the Fundamental Research Funds for the Central Universities(3072023GIP2401).
文摘In this paper,we consider the initial boundary value problem for the 2-D hyperbolic viscous Cahn-Hilliard equation.Firstly,we prove the existence and uniqueness of the local solution by the Galerkin method and contraction mapping principle.Then,using the potential well theory,we study the global well-posedness of the solution with initial data at different levels of initial energy,i.e.,subcritical initial energy,critical initial energy and arbitrary positive initial energy.For subcritical initial energy,we prove the global existence,asymptotic behavior and finite time blowup of the solution.Moreover,we extend these results to the critical initial energy using the scaling technique.For arbitrary positive initial energy,including the sup-critical initial energy,we obtain the sufficient conditions for finite time blow-up of the solution.As a further study for estimating the blowup time,we give a unified expression of the lower bound of blowup time for all three initial energy levels and estimate the upper bound of blowup time for subcritical and critical initial energy.
基金supported by grants from Key Research&Development Project of Nanhua Biomedical Co.,Ltd.(No.H202191490139)National Natural Science Foundation of China(No.31872866)+1 种基金China Postdoctoral Science Foundation(Nos.2021M701160 and 2022M721101)Funds of Hunan university(521119400156).
文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the pathogen responsible for coronavirus disease 2019(COVID-19),continues to evolve,giving rise to more variants and global reinfections.Previous research has demonstrated that barcode segments can effectively and cost-efficiently identify specific species within closely related populations.In this study,we designed and tested RNA barcode segments based on genetic evolutionary relationships to facilitate the efficient and accurate identification of SARS-CoV-2 from extensive virus samples,including human coronaviruses(HCoVs)and SARSr-CoV-2 lineages.Nucleotide sequences sourced from NCBI and GISAID were meticulously selected and curated to construct training sets,encompassing 1733 complete genome sequences of HCoVs and SARSr-CoV-2 lineages.Through genetic-level species testing,we validated the accuracy and reliability of the barcode segments for identifying SARS-CoV-2.Subsequently,75 main and subordinate species-specific barcode segments for SARS-CoV-2,located in ORF1ab,S,E,ORF7a,and N coding sequences,were intercepted and screened based on single-nucleotide polymorphism sites and weighted scores.Post-testing,these segments exhibited high recall rates(nearly 100%),specificity(almost 30%at the nucleotide level),and precision(100%)performance on identification.They were eventually visualized using one and two-dimensional combined barcodes and deposited in an online database(http://virusbarcodedatabase.top/).The successful integration of barcoding technology in SARS-CoV-2 identification provides valuable insights for future studies involving complete genome sequence polymorphism analysis.Moreover,this cost-effective and efficient identification approach also provides valuable reference for future research endeavors related to virus surveillance.
