The objective of this study was to determine the genotype effects of the bovine insulin-like growth factorⅠ(IGF-Ⅰ)and its binding protein 3(IGFBP3)genes on growth and development traits in beef cows,including 130 Ch...The objective of this study was to determine the genotype effects of the bovine insulin-like growth factorⅠ(IGF-Ⅰ)and its binding protein 3(IGFBP3)genes on growth and development traits in beef cows,including 130 Chinese Simmental,42 Nanyang,and 47 Luxi Yellow cattle.Sequence variations in the bovine IGF-Ⅰand IGFBP3 genes were investigated by single strand conformation polymorphism(SSCP).SSCPs were detected in 6 fragments,which is the 5'-flanking region,the 2nd exon,the 5th exon,and the 5th intron of the IGF-1 gene,and the 2nd exon,the 3rd exon of the 1GFBP3 gene.Two polymorphisms,an A-to-G transition in the 2rid exon of the IGF-Ⅰgene and a T-to-C transition in the 2rid exon of IGFBP3 gene were detected in 3 breeds.The allele frequencies of 2 polymorphisms were 0.0411(A),0.9589(B),and 0.7237(A),0.2763(B),respectively.These 2 loci were analyzed to associate with body weight,height at withers,body length,heart girth,rump width,and beef production index(BPI)at 0,6,12,24,and 36-month old.The IGFBP3 locus was shown to be associated with rump width,heart girth at 24-month and 36-month.Animals with BB genotype had higher rump width(24.86±0.47)cm at 24-month and(27.50±0.63)em at 36-month.The heart girth was highest for the individuals with BB genotype(171.33±1.84)cm and higher than those with AB genotype(166.68±1.13)cm(P〈0.05)at 36-month.展开更多
基金funded by the National Key Technology R&D Program of the China(2006BAD01A10,2006BAD14B07,2006BAD04A16)the National High Technology Research and Development Program of China(2006AA10Z197)
文摘The objective of this study was to determine the genotype effects of the bovine insulin-like growth factorⅠ(IGF-Ⅰ)and its binding protein 3(IGFBP3)genes on growth and development traits in beef cows,including 130 Chinese Simmental,42 Nanyang,and 47 Luxi Yellow cattle.Sequence variations in the bovine IGF-Ⅰand IGFBP3 genes were investigated by single strand conformation polymorphism(SSCP).SSCPs were detected in 6 fragments,which is the 5'-flanking region,the 2nd exon,the 5th exon,and the 5th intron of the IGF-1 gene,and the 2nd exon,the 3rd exon of the 1GFBP3 gene.Two polymorphisms,an A-to-G transition in the 2rid exon of the IGF-Ⅰgene and a T-to-C transition in the 2rid exon of IGFBP3 gene were detected in 3 breeds.The allele frequencies of 2 polymorphisms were 0.0411(A),0.9589(B),and 0.7237(A),0.2763(B),respectively.These 2 loci were analyzed to associate with body weight,height at withers,body length,heart girth,rump width,and beef production index(BPI)at 0,6,12,24,and 36-month old.The IGFBP3 locus was shown to be associated with rump width,heart girth at 24-month and 36-month.Animals with BB genotype had higher rump width(24.86±0.47)cm at 24-month and(27.50±0.63)em at 36-month.The heart girth was highest for the individuals with BB genotype(171.33±1.84)cm and higher than those with AB genotype(166.68±1.13)cm(P〈0.05)at 36-month.
